ID   TRN2_DATST              Reviewed;         260 AA.
AC   P50163;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=Tropinone reductase 2;
DE            EC=1.1.1.236;
DE   AltName: Full=Tropinone reductase II;
DE            Short=TR-II;
GN   Name=TR2;
OS   Datura stramonium (Jimsonweed) (Common thornapple).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Datureae;
OC   Datura.
OX   NCBI_TaxID=4076;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Root;
RX   MEDLINE=94022421; PubMed=8415746; DOI=10.1073/pnas.90.20.9591;
RA   Nakajima K., Hashimoto T., Yamada Y.;
RT   "Two tropinone reductases with different stereospecificities are
RT   short-chain dehydrogenases evolved from a common ancestor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:9591-9595(1993).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX   MEDLINE=98226735; PubMed=9560196; DOI=10.1073/pnas.95.9.4876;
RA   Nakajima K., Yamashita A., Akama H., Nakatsu T., Kato H.,
RA   Hashimoto T., Oda J., Yamada Y.;
RT   "Crystal structures of two tropinone reductases: different reaction
RT   stereospecificities in the same protein fold.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:4876-4881(1998).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH NADP AND
RP   SUBSTRATE.
RX   MEDLINE=99316165; PubMed=10387002; DOI=10.1021/bi9825044;
RA   Yamashita A., Kato H., Wakatsuki S., Tomizaki T., Nakatsu T.,
RA   Nakajima K., Hashimoto T., Yamada Y., Oda J.;
RT   "Structure of tropinone reductase-II complexed with NADP+ and
RT   pseudotropine at 1.9 A resolution: implication for stereospecific
RT   substrate binding and catalysis.";
RL   Biochemistry 38:7630-7637(1999).
CC   -!- FUNCTION: Catalyzes the stereospecific reduction of tropinone to
CC       pseudotropine.
CC   -!- CATALYTIC ACTIVITY: Pseudotropine + NADP(+) = tropinone + NADPH.
CC   -!- PATHWAY: Alkaloid biosynthesis; tropane alkaloid biosynthesis.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; L20474; AAA33282.1; -; mRNA.
DR   PIR; B48674; B48674.
DR   PDB; 1IPE; X-ray; 2.50 A; A/B=2-260.
DR   PDB; 1IPF; X-ray; 2.50 A; A/B=2-260.
DR   PDB; 2AE1; X-ray; 2.30 A; A=1-260.
DR   PDB; 2AE2; X-ray; 1.90 A; A/B=1-260.
DR   PDBsum; 1IPE; -.
DR   PDBsum; 1IPF; -.
DR   PDBsum; 2AE1; -.
DR   PDBsum; 2AE2; -.
DR   BioCyc; MetaCyc:MON-13851; -.
DR   BRENDA; 1.1.1.236; 20628.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0050358; F:tropinone reductase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; NADP; Oxidoreductase.
FT   CHAIN         1    260       Tropinone reductase 2.
FT                                /FTId=PRO_0000054786.
FT   NP_BIND      18     41       NADP.
FT   NP_BIND     192    196       NADP.
FT   ACT_SITE    159    159       Proton acceptor.
FT   BINDING     146    146       Substrate.
FT   STRAND       11     15
FT   HELIX        20     30
FT   TURN         31     33
FT   STRAND       35     41
FT   HELIX        43     55
FT   STRAND       60     64
FT   HELIX        70     83
FT   TURN         84     86
FT   STRAND       90     93
FT   HELIX       103    105
FT   HELIX       108    118
FT   HELIX       120    134
FT   STRAND      137    144
FT   HELIX       147    149
FT   HELIX       157    176
FT   HELIX       178    180
FT   STRAND      182    189
FT   HELIX       204    215
FT   STRAND      217    219
FT   HELIX       225    236
FT   HELIX       238    240
FT   STRAND      247    251
FT   HELIX       254    256
SQ   SEQUENCE   260 AA;  28311 MW;  2DBF4963B2CCA303 CRC64;
     MAGRWNLEGC TALVTGGSRG IGYGIVEELA SLGASVYTCS RNQKELNDCL TQWRSKGFKV
     EASVCDLSSR SERQELMNTV ANHFHGKLNI LVNNAGIVIY KEAKDYTVED YSLIMSINFE
     AAYHLSVLAH PFLKASERGN VVFISSVSGA LAVPYEAVYG ATKGAMDQLT RCLAFEWAKD
     NIRVNGVGPG VIATSLVEMT IQDPEQKENL NKLIDRCALR RMGEPKELAA MVAFLCFPAA
     SYVTGQIIYV DGGLMANCGF
//