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Protein

Tropinone reductase 2

Gene

TR2

Organism
Datura stramonium (Jimsonweed) (Common thornapple)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific reduction of tropinone to pseudotropine.

Catalytic activityi

Pseudotropine + NADP+ = tropinone + NADPH.

Pathwayi: tropane alkaloid biosynthesis

This protein is involved in the pathway tropane alkaloid biosynthesis, which is part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tropane alkaloid biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146Substrate1 Publication1
Active sitei159Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 41NADP1 PublicationAdd BLAST24
Nucleotide bindingi192 – 196NADP1 Publication5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13851.
BRENDAi1.1.1.236. 1839.
SABIO-RKP50163.
UniPathwayiUPA00330.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropinone reductase 2 (EC:1.1.1.236)
Alternative name(s):
Tropinone reductase II
Short name:
TR-II
Gene namesi
Name:TR2
OrganismiDatura stramonium (Jimsonweed) (Common thornapple)
Taxonomic identifieri4076 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeDatureaeDatura

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547861 – 260Tropinone reductase 2Add BLAST260

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 16Combined sources6
Helixi20 – 31Combined sources12
Beta strandi35 – 41Combined sources7
Helixi43 – 55Combined sources13
Beta strandi59 – 64Combined sources6
Helixi70 – 83Combined sources14
Turni84 – 86Combined sources3
Beta strandi90 – 93Combined sources4
Helixi103 – 105Combined sources3
Helixi108 – 118Combined sources11
Helixi120 – 135Combined sources16
Beta strandi137 – 144Combined sources8
Helixi147 – 149Combined sources3
Helixi157 – 176Combined sources20
Helixi178 – 180Combined sources3
Beta strandi182 – 189Combined sources8
Helixi195 – 200Combined sources6
Helixi204 – 215Combined sources12
Beta strandi217 – 219Combined sources3
Helixi225 – 236Combined sources12
Helixi238 – 240Combined sources3
Beta strandi247 – 251Combined sources5
Helixi254 – 256Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IPEX-ray2.50A/B2-260[»]
1IPFX-ray2.50A/B2-260[»]
2AE1X-ray2.30A1-260[»]
2AE2X-ray1.90A/B1-260[»]
ProteinModelPortaliP50163.
SMRiP50163.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50163.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK05354.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRWNLEGC TALVTGGSRG IGYGIVEELA SLGASVYTCS RNQKELNDCL
60 70 80 90 100
TQWRSKGFKV EASVCDLSSR SERQELMNTV ANHFHGKLNI LVNNAGIVIY
110 120 130 140 150
KEAKDYTVED YSLIMSINFE AAYHLSVLAH PFLKASERGN VVFISSVSGA
160 170 180 190 200
LAVPYEAVYG ATKGAMDQLT RCLAFEWAKD NIRVNGVGPG VIATSLVEMT
210 220 230 240 250
IQDPEQKENL NKLIDRCALR RMGEPKELAA MVAFLCFPAA SYVTGQIIYV
260
DGGLMANCGF
Length:260
Mass (Da):28,311
Last modified:October 1, 1996 - v1
Checksum:i2DBF4963B2CCA303
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20474 mRNA. Translation: AAA33282.1.
PIRiB48674.

Genome annotation databases

KEGGiag:AAA33282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20474 mRNA. Translation: AAA33282.1.
PIRiB48674.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IPEX-ray2.50A/B2-260[»]
1IPFX-ray2.50A/B2-260[»]
2AE1X-ray2.30A1-260[»]
2AE2X-ray1.90A/B1-260[»]
ProteinModelPortaliP50163.
SMRiP50163.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA33282.

Phylogenomic databases

KOiK05354.

Enzyme and pathway databases

UniPathwayiUPA00330.
BioCyciMetaCyc:MONOMER-13851.
BRENDAi1.1.1.236. 1839.
SABIO-RKP50163.

Miscellaneous databases

EvolutionaryTraceiP50163.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRN2_DATST
AccessioniPrimary (citable) accession number: P50163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.