ID GBG4_MOUSE Reviewed; 75 AA. AC P50153; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 27-MAR-2024, entry version 175. DE RecName: Full=Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4; DE Flags: Precursor; GN Name=Gng4; Synonyms=Gngt4; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. RC TISSUE=Brain; RX PubMed=7488078; DOI=10.1006/bbrc.1995.2600; RA Kalyanaraman S., Kalyanaraman V., Gautam N.; RT "A brain-specific G protein gamma subunit."; RL Biochem. Biophys. Res. Commun. 216:126-132(1995). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=9570961; DOI=10.1006/geno.1998.5223; RA Kalyanaraman S., Copeland N.G., Gilbert D.G., Jenkins N.A., Gautam N.; RT "Structure and chromosomal localization of mouse G protein subunit gamma4 RT gene."; RL Genomics 49:147-151(1998). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Eye; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brain; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). RN [5] RP INTERACTION WITH KCNK1. RX PubMed=24496152; DOI=10.1038/ncomms4227; RA Hwang E.M., Kim E., Yarishkin O., Woo D.H., Han K.S., Park N., Bae Y., RA Woo J., Kim D., Park M., Lee C.J., Park J.Y.; RT "A disulphide-linked heterodimer of TWIK-1 and TREK-1 mediates passive RT conductance in astrocytes."; RL Nat. Commun. 5:3227-3227(2014). CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved CC as a modulator or transducer in various transmembrane signaling CC systems. The beta and gamma chains are required for the GTPase CC activity, for replacement of GDP by GTP, and for G protein-effector CC interaction. {ECO:0000305}. CC -!- SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. CC Interacts with beta-1 and beta-2, but not with beta-3 (By similarity). CC Interacts with KCNK1. {ECO:0000250|UniProtKB:P50150, CC ECO:0000269|PubMed:24496152}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}. CC -!- TISSUE SPECIFICITY: Brain. {ECO:0000269|PubMed:7488078}. CC -!- SIMILARITY: Belongs to the G protein gamma family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U37527; AAB93460.1; -; mRNA. DR EMBL; AF038594; AAC40090.1; -; Genomic_DNA. DR EMBL; AF038593; AAC40090.1; JOINED; Genomic_DNA. DR EMBL; BC016506; AAH16506.1; -; mRNA. DR CCDS; CCDS36598.1; -. DR PIR; JC4339; JC4339. DR RefSeq; NP_001289926.1; NM_001302997.1. DR RefSeq; NP_034447.1; NM_010317.3. DR AlphaFoldDB; P50153; -. DR SMR; P50153; -. DR BioGRID; 199990; 1. DR DIP; DIP-494N; -. DR IntAct; P50153; 1. DR STRING; 10090.ENSMUSP00000021734; -. DR iPTMnet; P50153; -. DR PhosphoSitePlus; P50153; -. DR MaxQB; P50153; -. DR PaxDb; 10090-ENSMUSP00000021734; -. DR PeptideAtlas; P50153; -. DR ProteomicsDB; 272936; -. DR Antibodypedia; 34696; 105 antibodies from 24 providers. DR DNASU; 14706; -. DR Ensembl; ENSMUST00000021734.9; ENSMUSP00000021734.8; ENSMUSG00000021303.15. DR GeneID; 14706; -. DR KEGG; mmu:14706; -. DR UCSC; uc007pml.2; mouse. DR AGR; MGI:102703; -. DR CTD; 2786; -. DR MGI; MGI:102703; Gng4. DR VEuPathDB; HostDB:ENSMUSG00000021303; -. DR eggNOG; KOG4119; Eukaryota. DR GeneTree; ENSGT01100000263497; -. DR HOGENOM; CLU_168377_0_1_1; -. DR InParanoid; P50153; -. DR OMA; KESMSNN; -. DR OrthoDB; 2872810at2759; -. DR PhylomeDB; P50153; -. DR TreeFam; TF319909; -. DR Reactome; R-MMU-1296041; Activation of G protein gated Potassium channels. DR Reactome; R-MMU-202040; G-protein activation. DR Reactome; R-MMU-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion. DR Reactome; R-MMU-392170; ADP signalling through P2Y purinoceptor 12. DR Reactome; R-MMU-392451; G beta:gamma signalling through PI3Kgamma. DR Reactome; R-MMU-392851; Prostacyclin signalling through prostacyclin receptor. DR Reactome; R-MMU-400042; Adrenaline,noradrenaline inhibits insulin secretion. DR Reactome; R-MMU-4086398; Ca2+ pathway. DR Reactome; R-MMU-416476; G alpha (q) signalling events. DR Reactome; R-MMU-416482; G alpha (12/13) signalling events. DR Reactome; R-MMU-418217; G beta:gamma signalling through PLC beta. DR Reactome; R-MMU-418555; G alpha (s) signalling events. DR Reactome; R-MMU-418592; ADP signalling through P2Y purinoceptor 1. DR Reactome; R-MMU-418594; G alpha (i) signalling events. DR Reactome; R-MMU-418597; G alpha (z) signalling events. DR Reactome; R-MMU-420092; Glucagon-type ligand receptors. DR Reactome; R-MMU-428930; Thromboxane signalling through TP receptor. DR Reactome; R-MMU-432040; Vasopressin regulates renal water homeostasis via Aquaporins. DR Reactome; R-MMU-456926; Thrombin signalling through proteinase activated receptors (PARs). DR Reactome; R-MMU-500657; Presynaptic function of Kainate receptors. DR Reactome; R-MMU-6814122; Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding. DR Reactome; R-MMU-8964315; G beta:gamma signalling through BTK. DR Reactome; R-MMU-8964616; G beta:gamma signalling through CDC42. DR Reactome; R-MMU-9009391; Extra-nuclear estrogen signaling. DR Reactome; R-MMU-9634597; GPER1 signaling. DR Reactome; R-MMU-997272; Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits. DR BioGRID-ORCS; 14706; 1 hit in 78 CRISPR screens. DR PRO; PR:P50153; -. DR Proteomes; UP000000589; Chromosome 13. DR RNAct; P50153; Protein. DR Bgee; ENSMUSG00000021303; Expressed in facial nucleus and 158 other cell types or tissues. DR GO; GO:0005834; C:heterotrimeric G-protein complex; ISA:MGI. DR GO; GO:0031681; F:G-protein beta-subunit binding; IBA:GO_Central. DR GO; GO:0003924; F:GTPase activity; ISA:MGI. DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISA:MGI. DR GO; GO:0030308; P:negative regulation of cell growth; ISO:MGI. DR CDD; cd00068; GGL; 1. DR Gene3D; 4.10.260.10; Transducin (heterotrimeric G protein), gamma chain; 1. DR InterPro; IPR015898; G-protein_gamma-like_dom. DR InterPro; IPR036284; GGL_sf. DR InterPro; IPR001770; Gprotein-gamma. DR PANTHER; PTHR13809:SF21; GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)_G(S)_G(O) SUBUNIT GAMMA-4; 1. DR PANTHER; PTHR13809; GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT; 1. DR Pfam; PF00631; G-gamma; 1. DR PRINTS; PR00321; GPROTEING. DR SMART; SM01224; G_gamma; 1. DR SMART; SM00224; GGL; 1. DR SUPFAM; SSF48670; Transducin (heterotrimeric G protein), gamma chain; 1. DR PROSITE; PS50058; G_PROTEIN_GAMMA; 1. DR Genevisible; P50153; MM. PE 1: Evidence at protein level; KW Cell membrane; Lipoprotein; Membrane; Methylation; Prenylation; KW Reference proteome; Transducer. FT CHAIN 1..72 FT /note="Guanine nucleotide-binding protein G(I)/G(S)/G(O) FT subunit gamma-4" FT /id="PRO_0000012623" FT PROPEP 73..75 FT /note="Removed in mature form" FT /evidence="ECO:0000250" FT /id="PRO_0000012624" FT MOD_RES 72 FT /note="Cysteine methyl ester" FT /evidence="ECO:0000250" FT LIPID 72 FT /note="S-geranylgeranyl cysteine" FT /evidence="ECO:0000250" SQ SEQUENCE 75 AA; 8405 MW; 42FAEF47311FA195 CRC64; MKEGMSNNST TSISQARKAV EQLKMEACMD RVKVSQAASD LLAYCEAHVR EDPLIIPVPA SENPFREKKF FCTIL //