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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4

Gene

GNG4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.Curated

GO - Molecular functioni

GO - Biological processi

  • cellular response to glucagon stimulus Source: Reactome
  • G-protein coupled receptor signaling pathway Source: InterPro
  • negative regulation of cell growth Source: BHF-UCL
  • regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168243-MONOMER.
ReactomeiR-HSA-1296041. Activation of G protein gated Potassium channels.
R-HSA-163359. Glucagon signaling in metabolic regulation.
R-HSA-202040. G-protein activation.
R-HSA-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-HSA-392170. ADP signalling through P2Y purinoceptor 12.
R-HSA-392451. G beta:gamma signalling through PI3Kgamma.
R-HSA-392851. Prostacyclin signalling through prostacyclin receptor.
R-HSA-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-HSA-4086398. Ca2+ pathway.
R-HSA-416476. G alpha (q) signalling events.
R-HSA-416482. G alpha (12/13) signalling events.
R-HSA-418217. G beta:gamma signalling through PLC beta.
R-HSA-418555. G alpha (s) signalling events.
R-HSA-418592. ADP signalling through P2Y purinoceptor 1.
R-HSA-418594. G alpha (i) signalling events.
R-HSA-418597. G alpha (z) signalling events.
R-HSA-420092. Glucagon-type ligand receptors.
R-HSA-428930. Thromboxane signalling through TP receptor.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-HSA-500657. Presynaptic function of Kainate receptors.
R-HSA-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-HSA-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
SignaLinkiP50150.
SIGNORiP50150.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
Gene namesi
Name:GNG4
Synonyms:GNGT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:4407. GNG4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • heterotrimeric G-protein complex Source: InterPro
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi2786.
OpenTargetsiENSG00000168243.
ENSG00000282972.
PharmGKBiPA28786.

Polymorphism and mutation databases

BioMutaiGNG4.
DMDMi1730219.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000126211 – 72Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4Add BLAST72
PropeptideiPRO_000001262273 – 75Removed in mature form3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72Cysteine methyl ester1 Publication1
Lipidationi72S-geranylgeranyl cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP50150.
MaxQBiP50150.
PaxDbiP50150.
PeptideAtlasiP50150.
PRIDEiP50150.

PTM databases

PhosphoSitePlusiP50150.

Expressioni

Tissue specificityi

Brain, kidney, pancreas, skeletal muscle and faintly in cardiac muscle.

Gene expression databases

BgeeiENSG00000168243.
CleanExiHS_GNG4.
ExpressionAtlasiP50150. baseline and differential.
GenevisibleiP50150. HS.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with beta-1 and beta-2, but not with beta-3 (PubMed:7665596). Interacts with KCNK1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnk2Q920B63EBI-6395970,EBI-6530063From a different organism.

Protein-protein interaction databases

BioGridi109048. 18 interactors.
IntActiP50150. 1 interactor.
MINTiMINT-5004317.
STRINGi9606.ENSP00000355556.

Structurei

3D structure databases

ProteinModelPortaliP50150.
SMRiP50150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G protein gamma family.Curated

Phylogenomic databases

eggNOGiKOG4119. Eukaryota.
ENOG41122JF. LUCA.
GeneTreeiENSGT00760000119218.
HOVERGENiHBG014983.
InParanoidiP50150.
KOiK04541.
OMAiKEGMSSN.
OrthoDBiEOG091G17J0.
PhylomeDBiP50150.
TreeFamiTF319909.

Family and domain databases

CDDicd00068. GGL. 1 hit.
Gene3Di4.10.260.10. 1 hit.
InterProiIPR015898. G-protein_gamma-like_dom.
IPR001770. Gprotein-gamma.
[Graphical view]
PANTHERiPTHR13809. PTHR13809. 1 hit.
PfamiPF00631. G-gamma. 1 hit.
[Graphical view]
PRINTSiPR00321. GPROTEING.
SMARTiSM00224. GGL. 1 hit.
[Graphical view]
SUPFAMiSSF48670. SSF48670. 1 hit.
PROSITEiPS50058. G_PROTEIN_GAMMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEGMSNNST TSISQARKAV EQLKMEACMD RVKVSQAAAD LLAYCEAHVR
60 70
EDPLIIPVPA SENPFREKKF FCTIL
Length:75
Mass (Da):8,389
Last modified:October 1, 1996 - v1
Checksum:i42FAEF4728F83FF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31382 mRNA. Translation: AAC50204.1.
AF493872 mRNA. Translation: AAM12586.1.
BC022485 mRNA. Translation: AAH22485.1.
CCDSiCCDS1607.1.
PIRiI39157.
RefSeqiNP_001092191.1. NM_001098721.1.
NP_001092192.1. NM_001098722.1.
NP_004476.1. NM_004485.3.
XP_006711824.1. XM_006711761.2.
XP_011542469.1. XM_011544167.2.
UniGeneiHs.159711.

Genome annotation databases

EnsembliENST00000366597; ENSP00000355556; ENSG00000168243.
ENST00000366598; ENSP00000355557; ENSG00000168243.
ENST00000391854; ENSP00000375727; ENSG00000168243.
ENST00000450593; ENSP00000398629; ENSG00000168243.
ENST00000484517; ENSP00000482192; ENSG00000168243.
ENST00000634574; ENSP00000489027; ENSG00000282972.
ENST00000635006; ENSP00000489228; ENSG00000282972.
ENST00000635235; ENSP00000489454; ENSG00000282972.
ENST00000635387; ENSP00000489307; ENSG00000282972.
ENST00000635595; ENSP00000489194; ENSG00000282972.
GeneIDi2786.
KEGGihsa:2786.
UCSCiuc001hxe.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31382 mRNA. Translation: AAC50204.1.
AF493872 mRNA. Translation: AAM12586.1.
BC022485 mRNA. Translation: AAH22485.1.
CCDSiCCDS1607.1.
PIRiI39157.
RefSeqiNP_001092191.1. NM_001098721.1.
NP_001092192.1. NM_001098722.1.
NP_004476.1. NM_004485.3.
XP_006711824.1. XM_006711761.2.
XP_011542469.1. XM_011544167.2.
UniGeneiHs.159711.

3D structure databases

ProteinModelPortaliP50150.
SMRiP50150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109048. 18 interactors.
IntActiP50150. 1 interactor.
MINTiMINT-5004317.
STRINGi9606.ENSP00000355556.

PTM databases

PhosphoSitePlusiP50150.

Polymorphism and mutation databases

BioMutaiGNG4.
DMDMi1730219.

Proteomic databases

EPDiP50150.
MaxQBiP50150.
PaxDbiP50150.
PeptideAtlasiP50150.
PRIDEiP50150.

Protocols and materials databases

DNASUi2786.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000366597; ENSP00000355556; ENSG00000168243.
ENST00000366598; ENSP00000355557; ENSG00000168243.
ENST00000391854; ENSP00000375727; ENSG00000168243.
ENST00000450593; ENSP00000398629; ENSG00000168243.
ENST00000484517; ENSP00000482192; ENSG00000168243.
ENST00000634574; ENSP00000489027; ENSG00000282972.
ENST00000635006; ENSP00000489228; ENSG00000282972.
ENST00000635235; ENSP00000489454; ENSG00000282972.
ENST00000635387; ENSP00000489307; ENSG00000282972.
ENST00000635595; ENSP00000489194; ENSG00000282972.
GeneIDi2786.
KEGGihsa:2786.
UCSCiuc001hxe.5. human.

Organism-specific databases

CTDi2786.
DisGeNETi2786.
GeneCardsiGNG4.
HGNCiHGNC:4407. GNG4.
MIMi604388. gene.
neXtProtiNX_P50150.
OpenTargetsiENSG00000168243.
ENSG00000282972.
PharmGKBiPA28786.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4119. Eukaryota.
ENOG41122JF. LUCA.
GeneTreeiENSGT00760000119218.
HOVERGENiHBG014983.
InParanoidiP50150.
KOiK04541.
OMAiKEGMSSN.
OrthoDBiEOG091G17J0.
PhylomeDBiP50150.
TreeFamiTF319909.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000168243-MONOMER.
ReactomeiR-HSA-1296041. Activation of G protein gated Potassium channels.
R-HSA-163359. Glucagon signaling in metabolic regulation.
R-HSA-202040. G-protein activation.
R-HSA-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-HSA-392170. ADP signalling through P2Y purinoceptor 12.
R-HSA-392451. G beta:gamma signalling through PI3Kgamma.
R-HSA-392851. Prostacyclin signalling through prostacyclin receptor.
R-HSA-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-HSA-4086398. Ca2+ pathway.
R-HSA-416476. G alpha (q) signalling events.
R-HSA-416482. G alpha (12/13) signalling events.
R-HSA-418217. G beta:gamma signalling through PLC beta.
R-HSA-418555. G alpha (s) signalling events.
R-HSA-418592. ADP signalling through P2Y purinoceptor 1.
R-HSA-418594. G alpha (i) signalling events.
R-HSA-418597. G alpha (z) signalling events.
R-HSA-420092. Glucagon-type ligand receptors.
R-HSA-428930. Thromboxane signalling through TP receptor.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-HSA-500657. Presynaptic function of Kainate receptors.
R-HSA-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-HSA-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
SignaLinkiP50150.
SIGNORiP50150.

Miscellaneous databases

ChiTaRSiGNG4. human.
GenomeRNAii2786.
PROiP50150.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168243.
CleanExiHS_GNG4.
ExpressionAtlasiP50150. baseline and differential.
GenevisibleiP50150. HS.

Family and domain databases

CDDicd00068. GGL. 1 hit.
Gene3Di4.10.260.10. 1 hit.
InterProiIPR015898. G-protein_gamma-like_dom.
IPR001770. Gprotein-gamma.
[Graphical view]
PANTHERiPTHR13809. PTHR13809. 1 hit.
PfamiPF00631. G-gamma. 1 hit.
[Graphical view]
PRINTSiPR00321. GPROTEING.
SMARTiSM00224. GGL. 1 hit.
[Graphical view]
SUPFAMiSSF48670. SSF48670. 1 hit.
PROSITEiPS50058. G_PROTEIN_GAMMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBG4_HUMAN
AccessioniPrimary (citable) accession number: P50150
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.