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Protein

Guanine nucleotide-binding protein G(t) subunit alpha-2

Gene

Gnat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi181 – 1811MagnesiumBy similarity
Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi175 – 1817GTPBy similarity
Nucleotide bindingi200 – 2045GTPBy similarity
Nucleotide bindingi269 – 2724GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • detection of chemical stimulus involved in sensory perception of bitter taste Source: GO_Central
  • detection of light stimulus involved in visual perception Source: MGI
  • G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
  • phototransduction Source: MGI
  • positive regulation of cytosolic calcium ion concentration Source: Ensembl
  • response to light intensity Source: Ensembl
  • retinal cone cell development Source: MGI
  • visual perception Source: MGI
  • Wnt signaling pathway, calcium modulating pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-202040. G-protein activation.
R-MMU-4086398. Ca2+ pathway.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-2
Alternative name(s):
Transducin alpha-2 chain
Gene namesi
Name:Gnat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95779. Gnat2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • heterotrimeric G-protein complex Source: MGI
  • membrane Source: MGI
  • photoreceptor inner segment Source: MGI
  • photoreceptor outer segment Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 354353Guanine nucleotide-binding protein G(t) subunit alpha-2PRO_0000203741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP50149.
MaxQBiP50149.
PaxDbiP50149.
PRIDEiP50149.

PTM databases

iPTMnetiP50149.
PhosphoSiteiP50149.

Expressioni

Tissue specificityi

Retinal rod outer segment.

Gene expression databases

BgeeiP50149.
ExpressionAtlasiP50149. baseline and differential.
GenevisibleiP50149. MM.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP50149. 2 interactions.
STRINGi10090.ENSMUSP00000053818.

Structurei

3D structure databases

ProteinModelPortaliP50149.
SMRiP50149. Positions 14-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP50149.
KOiK04631.
OMAiECLEYKA.
OrthoDBiEOG72C50B.
PhylomeDBiP50149.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGISAEDK ELARRSKELE KKLQEDADKE AKTVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHQDG YSPEECLEFK SVIYGNVLQS ILAIIRAMST LGIDYAEPSC
110 120 130 140 150
ADAGRQLNNL ADSTEEGTMP PELVDVIRKL WKDGGVQACF DRAAEFQLND
160 170 180 190 200
SASYYLNQLD RITDPNYLPN EQDVLRSRVK TTGIIETKFS VKDLNFRMFD
210 220 230 240 250
VGGQRSERKK WIHCFEGVTC IIFCAALSAY DMVLVEDDEV NRMHESLHLF
260 270 280 290 300
NSICNHKFFA ATSIVLFLNK KDLFEEKIKK VHLSICFPEY DGNNSYEDAG
310 320 330 340 350
NYIKSQFLDL NMRKDVKEIY SHMTCATDTQ NVKFVFDAVT DIIIKENLKD

CGLF
Length:354
Mass (Da):40,118
Last modified:January 23, 2007 - v2
Checksum:i6A14B9B152F14F4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10666 mRNA. Translation: AAC37650.1.
BC016272 mRNA. Translation: AAH16272.1.
CCDSiCCDS17750.1.
RefSeqiNP_032167.1. NM_008141.3.
XP_006501071.1. XM_006501008.2.
UniGeneiMm.439652.

Genome annotation databases

EnsembliENSMUST00000058669; ENSMUSP00000053818; ENSMUSG00000009108.
GeneIDi14686.
KEGGimmu:14686.
UCSCiuc008qyc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10666 mRNA. Translation: AAC37650.1.
BC016272 mRNA. Translation: AAH16272.1.
CCDSiCCDS17750.1.
RefSeqiNP_032167.1. NM_008141.3.
XP_006501071.1. XM_006501008.2.
UniGeneiMm.439652.

3D structure databases

ProteinModelPortaliP50149.
SMRiP50149. Positions 14-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50149. 2 interactions.
STRINGi10090.ENSMUSP00000053818.

PTM databases

iPTMnetiP50149.
PhosphoSiteiP50149.

Proteomic databases

EPDiP50149.
MaxQBiP50149.
PaxDbiP50149.
PRIDEiP50149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058669; ENSMUSP00000053818; ENSMUSG00000009108.
GeneIDi14686.
KEGGimmu:14686.
UCSCiuc008qyc.2. mouse.

Organism-specific databases

CTDi2780.
MGIiMGI:95779. Gnat2.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP50149.
KOiK04631.
OMAiECLEYKA.
OrthoDBiEOG72C50B.
PhylomeDBiP50149.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-202040. G-protein activation.
R-MMU-4086398. Ca2+ pathway.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

PROiP50149.
SOURCEiSearch...

Gene expression databases

BgeeiP50149.
ExpressionAtlasiP50149. baseline and differential.
GenevisibleiP50149. MM.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of cone transducin, Gz alpha, and other G-protein alpha-subunit messenger ribonucleic acids in pancreatic islets."
    Zigman J.M., Westermark G.T., LaMendola J., Steiner D.F.
    Endocrinology 135:31-37(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Retina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiGNAT2_MOUSE
AccessioniPrimary (citable) accession number: P50149
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.