P50141 (GCH1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP cyclohydrolase 1
EC=3.5.4.16

Alternative name(s):
GTP cyclohydrolase I
Short name=GTP-CH-I

Gene names

Name:

GCH1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence By similarity.
Catalytic activity	GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.
Enzyme regulation	GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity. Zinc is required for catalytic activity. Inhibited by Mg2+ By similarity.
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.
Subunit structure	Toroid-shaped homodecamer, composed of two pentamers of five dimers By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	GTP cyclohydrolase 1		PRO_0000119477
Sites
	Metal binding	127	1	Zinc By similarity
	Metal binding	130	1	Zinc By similarity
	Metal binding	198	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50141 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F235D56B991D8C24	FASTA	236	26,115
	10 20 30 40 50 60 MAAARSCNGY ARREGPPSPK LGTEKPRVSA GSGGSGDGWR GERPRSEEDN ELSLPSLAAA 70 80 90 100 110 120 YTTILRALGE DPERQGLLKT PWRAATAMQF FTKGYQETIA DVLNDAIFDE DHDEMVIVKN 130 140 150 160 170 180 IDMFSLCEHH LVPFVGKVHI GYLPNKQVLG LSKLARIVEI YSRRLQVQER LTKQIAIAIT 190 200 210 220 230 EALQPAGVGV VIEATHMCMV MRGVQKMNSK TATSTMLGVF REDPKTREEF LTLIRS

References

[1]	"Homology cloning of GTP-cyclohydrolase I from various unrelated eukaryotes by reverse-transcription polymerase chain reaction using a general set of degenerate primers." Maier J., Witter K., Guetlich M., Ziegler I., Werner T., Ninnemann H. Biochem. Biophys. Res. Commun. 212:705-711(1995) [PubMed: 7542887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49267 mRNA. Translation: CAA89261.1.
IPI	IPI00582569.
PIR	I50646.
RefSeq	NP_990554.1. NM_205223.1.
UniGene	Gga.674.
3D structure databases
ProteinModelPortal	P50141.
SMR	P50141. Positions 43-236.
ModBase	Search...
Protein-protein interaction databases
STRING	P50141.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396146.
KEGG	gga:396146.
Organism-specific databases
CTD	2643.
Phylogenomic databases
eggNOG	veNOG11752.
GeneTree	ENSGT00390000013481.
HOGENOM	HBG370191.
HOVERGEN	HBG003136.
InParanoid	P50141.
OrthoDB	EOG4FR0SK.
PhylomeDB	P50141.
Family and domain databases
InterPro	IPR001474. GTP_CycHdrlase_I. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. IPR018234. GTP_CycHdrlase_I_CS. [Graphical view]
KO	K01495.
PANTHER	PTHR11109. GTP_cyclohydro_I. 1 hit.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
TIGRFAMs	TIGR00063. FolE. 1 hit.
PROSITE	PS00859. GTP_CYCLOHYDROL_1_1. 1 hit. PS00860. GTP_CYCLOHYDROL_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCH1_CHICK

Accession

Primary (citable) accession number: P50141

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program