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Protein

5-hydroxytryptamine receptor 2A

Gene

HTR2A

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction (By similarity).By similarity

GO - Molecular functioni

  1. serotonin receptor activity Source: InterPro

GO - Biological processi

  1. behavior Source: UniProtKB-KW
  2. regulation of behavior Source: InterPro
  3. regulation of hormone secretion Source: InterPro
  4. smooth muscle contraction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2A
Short name:
5-HT-2
Short name:
5-HT-2A
Alternative name(s):
Serotonin receptor 2A
Gene namesi
Name:HTR2A
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
ProteomesiUP000006718: Unplaced

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Cell projectiondendrite By similarity. Cell projectionaxon By similarity. Cytoplasmic vesicle By similarity. Membranecaveola By similarity
Note: Localizes to the postsynaptic thickening of axo-dendritic synapses.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575ExtracellularBy similarityAdd
BLAST
Transmembranei76 – 9924Helical; Name=1By similarityAdd
BLAST
Topological domaini100 – 11011CytoplasmicBy similarityAdd
BLAST
Transmembranei111 – 13222Helical; Name=2By similarityAdd
BLAST
Topological domaini133 – 14816ExtracellularBy similarityAdd
BLAST
Transmembranei149 – 17123Helical; Name=3By similarityAdd
BLAST
Topological domaini172 – 19120CytoplasmicBy similarityAdd
BLAST
Transmembranei192 – 21524Helical; Name=4By similarityAdd
BLAST
Topological domaini216 – 23318ExtracellularBy similarityAdd
BLAST
Transmembranei234 – 25421Helical; Name=5By similarityAdd
BLAST
Topological domaini255 – 32470CytoplasmicBy similarityAdd
BLAST
Transmembranei325 – 34622Helical; Name=6By similarityAdd
BLAST
Topological domaini347 – 36216ExtracellularBy similarityAdd
BLAST
Transmembranei363 – 38422Helical; Name=7By similarityAdd
BLAST
Topological domaini385 – 47187CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. axon Source: UniProtKB-SubCell
  2. caveola Source: UniProtKB-SubCell
  3. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  4. dendritic shaft Source: InterPro
  5. integral component of plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4714715-hydroxytryptamine receptor 2APRO_0000068947Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi148 ↔ 227PROSITE-ProRule annotation
Modified residuei280 – 2801PhosphoserineBy similarity
Disulfide bondi349 ↔ 353PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling (By similarity).By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000036319.

Structurei

3D structure databases

ProteinModelPortaliP50128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni155 – 1606Agonist bindingBy similarity
Regioni336 – 3405Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi172 – 1743DRY motif; important for ligand-induced conformation changesBy similarity
Motifi376 – 3805NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity
Motifi469 – 4713PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG247243.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP50128.
KOiK04157.

Family and domain databases

InterProiIPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF30. PTHR24247:SF30. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50128-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDILCEENTS LSSTTNSLMQ LNEDTRLYSN DFNSGEANTS DAFNWTVESE
60 70 80 90 100
NRTNLSCEGC LSPSCLSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS
110 120 130 140 150
LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV
160 170 180 190 200
WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHSRFNSRT KAFLKIIAVW
210 220 230 240 250
TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM
260 270 280 290 300
VITYFLTIKS LQKEATLCVS DLGTRAKLAS FSFLPQSSLS SEKLFQRSIH
310 320 330 340 350
RDPGSYTGRR TMQSISNEQK ACKVLGIVFF LFVVMWCPFF ITNIMAVICK
360 370 380 390 400
ESCNEDVIGA LLNVFVWIGY LSSAVNPLVY TLFNKTYRSA FSRYIQCQYK
410 420 430 440 450
ENKKPLQLIL VNTIPALAYK SSQLQMGQKK NSKQDAKTTD NDCSMVALGK
460 470
QHSEDASKDN SDGVNEKVSC V
Length:471
Mass (Da):52,603
Last modified:October 1, 1996 - v1
Checksum:i300FFC84DE03D462
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78209 mRNA. Translation: AAB34691.1.
RefSeqiNP_001028138.1. NM_001032966.1.
UniGeneiMmu.3400.

Genome annotation databases

GeneIDi613032.
KEGGimcc:613032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78209 mRNA. Translation: AAB34691.1.
RefSeqiNP_001028138.1. NM_001032966.1.
UniGeneiMmu.3400.

3D structure databases

ProteinModelPortaliP50128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000036319.

Chemistry

BindingDBiP50128.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi613032.
KEGGimcc:613032.

Organism-specific databases

CTDi3356.

Phylogenomic databases

eggNOGiNOG247243.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP50128.
KOiK04157.

Miscellaneous databases

NextBioi19979833.

Family and domain databases

InterProiIPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF30. PTHR24247:SF30. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Species differences in 5-HT2A receptors: cloned pig and rhesus monkey 5-HT2A receptors reveal conserved transmembrane homology to the human rather than rat sequence."
    Johnson M.P., Baez M., Kursar J.D., Nelson D.L.
    Biochim. Biophys. Acta 1236:201-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry namei5HT2A_MACMU
AccessioniPrimary (citable) accession number: P50128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.