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Protein

5-hydroxytryptamine receptor 2A

Gene

HTR2A

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei229Hydrophobic barrier that decreases the speed of ligand binding and dissociationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2A
Short name:
5-HT-2
Short name:
5-HT-2A
Alternative name(s):
Serotonin receptor 2A
Gene namesi
Name:HTR2A
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75ExtracellularBy similarityAdd BLAST75
Transmembranei76 – 99Helical; Name=1By similarityAdd BLAST24
Topological domaini100 – 110CytoplasmicBy similarityAdd BLAST11
Transmembranei111 – 132Helical; Name=2By similarityAdd BLAST22
Topological domaini133 – 148ExtracellularBy similarityAdd BLAST16
Transmembranei149 – 171Helical; Name=3By similarityAdd BLAST23
Topological domaini172 – 191CytoplasmicBy similarityAdd BLAST20
Transmembranei192 – 215Helical; Name=4By similarityAdd BLAST24
Topological domaini216 – 233ExtracellularBy similarityAdd BLAST18
Transmembranei234 – 254Helical; Name=5By similarityAdd BLAST21
Topological domaini255 – 324CytoplasmicBy similarityAdd BLAST70
Transmembranei325 – 346Helical; Name=6By similarityAdd BLAST22
Topological domaini347 – 362ExtracellularBy similarityAdd BLAST16
Transmembranei363 – 384Helical; Name=7By similarityAdd BLAST22
Topological domaini385 – 471CytoplasmicBy similarityAdd BLAST87

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689471 – 4715-hydroxytryptamine receptor 2AAdd BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi51N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi148 ↔ 227PROSITE-ProRule annotation
Modified residuei280PhosphoserineBy similarity1
Disulfide bondi349 ↔ 353PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Interacts (via C-terminus) with MPDZ and PATJ. May interact (via C-terminus) with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling.By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000036319.

Chemistry databases

BindingDBiP50128.

Structurei

3D structure databases

ProteinModelPortaliP50128.
SMRiP50128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 160Agonist bindingBy similarity6
Regioni336 – 340Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi172 – 174DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi376 – 380NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5
Motifi469 – 471PDZ-bindingBy similarity3

Domaini

The PDZ domain-binding motif is involved in the interaction with PATJ, CASK, APBA1, DLG1 and DLG4.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP50128.
KOiK04157.

Family and domain databases

InterProiView protein in InterPro
IPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PANTHERiPTHR24247:SF154. PTHR24247:SF154. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P50128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDILCEENTS LSSTTNSLMQ LNEDTRLYSN DFNSGEANTS DAFNWTVESE
60 70 80 90 100
NRTNLSCEGC LSPSCLSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS
110 120 130 140 150
LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV
160 170 180 190 200
WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHSRFNSRT KAFLKIIAVW
210 220 230 240 250
TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM
260 270 280 290 300
VITYFLTIKS LQKEATLCVS DLGTRAKLAS FSFLPQSSLS SEKLFQRSIH
310 320 330 340 350
RDPGSYTGRR TMQSISNEQK ACKVLGIVFF LFVVMWCPFF ITNIMAVICK
360 370 380 390 400
ESCNEDVIGA LLNVFVWIGY LSSAVNPLVY TLFNKTYRSA FSRYIQCQYK
410 420 430 440 450
ENKKPLQLIL VNTIPALAYK SSQLQMGQKK NSKQDAKTTD NDCSMVALGK
460 470
QHSEDASKDN SDGVNEKVSC V
Length:471
Mass (Da):52,603
Last modified:October 1, 1996 - v1
Checksum:i300FFC84DE03D462
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78209 mRNA. Translation: AAB34691.1.
RefSeqiNP_001028138.1. NM_001032966.1.
UniGeneiMmu.3400.

Genome annotation databases

GeneIDi613032.
KEGGimcc:613032.

Similar proteinsi

Entry informationi

Entry namei5HT2A_MACMU
AccessioniPrimary (citable) accession number: P50128
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: August 30, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families