Reviewed, UniProtKB/Swiss-Prot P50127 (GGTA1_PIG)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide alpha-1,3-galactosyltransferase
    EC=2.4.1.87
Alternative name(s):
    UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
      Short name=Galactosyltransferase

Gene names

Name:

GGTA1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P50127-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P50127-2)

The sequence of this isoform differs from the canonical sequence as follows:
     27-39: SPEGSLFWIYQSK → R

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	N-acetyllactosaminide alpha-1,3-galactosyltransferase		PRO_0000157300
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 22	16	Signal-anchor for type II membrane protein Potential
	Topological domain	23 – 371	349	Lumenal Potential
Sites
	Metal binding	228	1	Manganese By similarity
	Metal binding	230	1	Manganese By similarity
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	296	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	27 – 39	13	SPEGS…IYQSK → R in isoform Short.		VSP_001806
Experimental info
	Sequence conflict	227	1	M → I in AAA58775. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CFC715E8D89993D4	FASTA	371	43,764
	10 20 30 40 50 60 MNVKGRVVLS MLLVSTVMVV FWEYINSPEG SLFWIYQSKN PEVGSSAQRG WWFPSWFNNG 70 80 90 100 110 120 THSYHEEEDA IGNEKEQRKE DNRGELPLVD WFNPEKRPEV VTITRWKAPV VWEGTYNRAV 130 140 150 160 170 180 LDNYYAKQKI TVGLTVFAVG RYIEHYLEEF LISANTYFMV GHKVIFYIMV DDISRMPLIE 190 200 210 220 230 240 LGPLRSFKVF EIKSEKRWQD ISMMRMKTIG EHILAHIQHE VDFLFCMDVD QVFQNNFGVE 250 260 270 280 290 300 TLGQSVAQLQ AWWYKAHPDE FTYERRKESA AYIPFGQGDF YYHAAIFGGT PTQVLNITQE 310 320 330 340 350 360 CFKGILQDKE NDIEAEWHDE SHLNKYFLLN KPTKILSPEY CWDYHIGMSV DIRIVKIAWQ 370 KKEYNLVRNN I
	Isoform Short. Checksum: EA032F93ECD24CB6	FASTA	359	42,396
	10 20 30 40 50 60 MNVKGRVVLS MLLVSTVMVV FWEYINRNPE VGSSAQRGWW FPSWFNNGTH SYHEEEDAIG 70 80 90 100 110 120 NEKEQRKEDN RGELPLVDWF NPEKRPEVVT ITRWKAPVVW EGTYNRAVLD NYYAKQKITV 130 140 150 160 170 180 GLTVFAVGRY IEHYLEEFLI SANTYFMVGH KVIFYIMVDD ISRMPLIELG PLRSFKVFEI 190 200 210 220 230 240 KSEKRWQDIS MMRMKTIGEH ILAHIQHEVD FLFCMDVDQV FQNNFGVETL GQSVAQLQAW 250 260 270 280 290 300 WYKAHPDEFT YERRKESAAY IPFGQGDFYY HAAIFGGTPT QVLNITQECF KGILQDKEND 310 320 330 340 350 IEAEWHDESH LNKYFLLNKP TKILSPEYCW DYHIGMSVDI RIVKIAWQKK EYNLVRNNI

References

[1]	"cDNA sequence and chromosome localization of pig alpha 1,3 galactosyltransferase." Strahan K.M., Gu F., Preece A.F., Gustavsson I., Andersson L., Gustafsson K. Immunogenetics 41:101-105(1995) [PubMed: 7528726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Yorkshire. Tissue: Spleen.
[2]	Sandrin M.S., Dabkowski P.L., Henning M.M., Mouhtouris E., McKenzie I.F.C. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver and Spleen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L36152 mRNA. Translation: AAA73558.1. L36535 mRNA. Translation: AAA58775.1.
PIR	I46583.
RefSeq	NP_998975.1.
UniGene	Ssc.8552
3D structure databases
HSSP	HSSP built from PDB template 1G93 based on UniProtKB P14769.
SMR	P50127. Positions 85-371.
ModBase	Search...
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Genome annotation databases
GeneID	396733.
KEGG	ssc:396733.
Organism-specific databases
CTD	396733.
Phylogenomic databases
HOVERGEN	P50127.
Enzyme and pathway databases
BRENDA	2.4.1.87. 249.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGTA1_PIG

Accession

Primary (citable) accession number: P50127

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)