Skip Header

Contribute Send feedback
Read comments (?) or add your own

P50127 (GGTA1_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetyllactosaminide alpha-1,3-galactosyltransferase
EC=2.4.1.87
Alternative name(s):
UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
Short name=Galactosyltransferase
Gene names
Name:GGTA1
OrganismSus scrofa (Pig) [Complete proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transfer of galactose from UDP-galactose to an acceptor molecule (R).

Catalytic activity

UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.

Cofactor

Manganese.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.

Domain

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similarities

Belongs to the glycosyltransferase 6 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P50127-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P50127-2)

The sequence of this isoform differs from the canonical sequence as follows:
     27-39: SPEGSLFWIYQSK → R

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371N-acetyllactosaminide alpha-1,3-galactosyltransferase
PRO_0000157300

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2216Helical; Signal-anchor for type II membrane protein; Potential
Topological domain23 – 371349Lumenal Potential

Sites

Metal binding2281Manganese By similarity
Metal binding2301Manganese By similarity

Amino acid modifications

Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation2961N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence27 – 3913SPEGS…IYQSK → R in isoform Short.
VSP_001806

Experimental info

Sequence conflict2271M → I in AAA58775. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: CFC715E8D89993D4

FASTA37143,764
        10         20         30         40         50         60 
MNVKGRVVLS MLLVSTVMVV FWEYINSPEG SLFWIYQSKN PEVGSSAQRG WWFPSWFNNG 

        70         80         90        100        110        120 
THSYHEEEDA IGNEKEQRKE DNRGELPLVD WFNPEKRPEV VTITRWKAPV VWEGTYNRAV 

       130        140        150        160        170        180 
LDNYYAKQKI TVGLTVFAVG RYIEHYLEEF LISANTYFMV GHKVIFYIMV DDISRMPLIE 

       190        200        210        220        230        240 
LGPLRSFKVF EIKSEKRWQD ISMMRMKTIG EHILAHIQHE VDFLFCMDVD QVFQNNFGVE 

       250        260        270        280        290        300 
TLGQSVAQLQ AWWYKAHPDE FTYERRKESA AYIPFGQGDF YYHAAIFGGT PTQVLNITQE 

       310        320        330        340        350        360 
CFKGILQDKE NDIEAEWHDE SHLNKYFLLN KPTKILSPEY CWDYHIGMSV DIRIVKIAWQ 

       370 
KKEYNLVRNN I

« Hide

Isoform Short [UniParc].

Checksum: EA032F93ECD24CB6
Show »

FASTA35942,396

References

[1]"cDNA sequence and chromosome localization of pig alpha 1,3 galactosyltransferase."
Strahan K.M., Gu F., Preece A.F., Gustavsson I., Andersson L., Gustafsson K.
Immunogenetics 41:101-105(1995) [PubMed: 7528726] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Yorkshire.
Tissue: Spleen.
[2]Sandrin M.S., Dabkowski P.L., Henning M.M., Mouhtouris E., McKenzie I.F.C.
Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver and Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L36152 mRNA. Translation: AAA73558.1.
L36535 mRNA. Translation: AAA58775.1.
PIRI46583.
RefSeqNP_998975.1. NM_213810.2.
UniGeneSsc.8552.

3D structure databases

ProteinModelPortalP50127.
SMRP50127. Positions 85-371.
ModBaseSearch...

Protein-protein interaction databases

STRINGP50127.

Protein family/group databases

CAZyGT6. Glycosyltransferase Family 6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000006069; ENSSSCP00000005915; ENSSSCG00000005518.
GeneID396733.
KEGGssc:396733.

Organism-specific databases

CTD14594.

Phylogenomic databases

HOVERGENHBG003563.
OMACMDVDQV.
OrthoDBEOG4C5CK3.

Enzyme and pathway databases

BRENDA2.4.1.87. 6170.

Family and domain databases

InterProIPR005076. Glyco_trans_6.
[Graphical view]
KOK00743.
PANTHERPTHR10462. Glyco_trans_6. 1 hit.
PfamPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGGTA1_PIG
AccessionPrimary (citable) accession number: P50127
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents