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Protein

Glutamyl aminopeptidase

Gene

Enpep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to have a role in the catabolic pathway of the renin-angiotensin system. Isoform 1 has aminopeptidase activity while isoform 2 does not.

Catalytic activityi

Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215SubstrateBy similarity1
Metal bindingi385Zinc; catalyticPROSITE-ProRule annotation1
Active sitei386Proton acceptorPROSITE-ProRule annotation1
Metal bindingi389Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi408Zinc; catalyticPROSITE-ProRule annotation1
Sitei471Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: RGD
  • metalloaminopeptidase activity Source: UniProtKB
  • peptide binding Source: RGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.003

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl aminopeptidase (EC:3.4.11.7)
Short name:
EAP
Alternative name(s):
Aminopeptidase A
Short name:
AP-A
CD_antigen: CD249
Gene namesi
Name:Enpep
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi621228 Enpep

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini40 – 945ExtracellularSequence analysisAdd BLAST906

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950981 – 945Glutamyl aminopeptidaseAdd BLAST945

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi236N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi546N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi584N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi601N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi640N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi669N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi754N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi766N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi792N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP50123
PRIDEiP50123

PTM databases

iPTMnetiP50123
PhosphoSitePlusiP50123

Expressioni

Tissue specificityi

Highest expression in kidney proximal tubules and ileum enterocytes. High expression also detected in liver and pituitary. Lower levels in heart, adrenal gland and brain. Not detected in aorta, lung or spleen. In heart, higher levels in ventricle than in atrium. Also expressed in glomerular mesangial cells.4 Publications

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP50123, 1 interactor
STRINGi10116.ENSRNOP00000064188

Structurei

3D structure databases

ProteinModelPortaliP50123
SMRiP50123
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 353Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046 Eukaryota
COG0308 LUCA
HOVERGENiHBG006616
InParanoidiP50123
KOiK11141
PhylomeDBiP50123

Family and domain databases

CDDicd09601 M1_APN_2, 1 hit
InterProiView protein in InterPro
IPR033508 Aminopeptidase_A
IPR024571 ERAP1-like_C_dom
IPR034016 M1_APN-typ
IPR001930 Peptidase_M1
IPR014782 Peptidase_M1_N
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF242 PTHR11533:SF242, 1 hit
PfamiView protein in Pfam
PF11838 ERAP1_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P50123-1) [UniParc]FASTAAdd to basket
Also known as: APAL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFAEEEPSK KYCIKGKHVA IICATVVAVG LIVGLSVGLT RSCEPGTTPA
60 70 80 90 100
PSNPPPHTST ALPPQDQNVC PDSDDESGEW KNFRLPDFIQ PVHYDLEVKV
110 120 130 140 150
LMEEDRYTGI VSISVNLSKD TRDLWLHIRE TRITKLPELR RPSGEQVPIR
160 170 180 190 200
RCFEYKKQEY VVIQAEEDLA ATSGDSVYRL TIEFEGWLNG SLVGFYRTTY
210 220 230 240 250
TEDGQTKSIA ATDHEPTDAR KSFPCFDEPN KKATYNISLI HPKEYSALSN
260 270 280 290 300
MPVEKKETLD NDWKKTTFMK SVPMSTYLVC FAVHQFTSIQ RTSRSGKPLT
310 320 330 340 350
VYVQPNQKQT AEYAANITKA VFDFFEDYFA MEYSLPKLDK IAIPDFGTGA
360 370 380 390 400
MENWGLVTYR ETNLLYDPLL SASSNQQRVA SVVAHELVHQ WFGNIVTMDW
410 420 430 440 450
WDDLWLNEGF ASFFEFLGVN HAEADWQMLS QVLLEDVLPV QEDDSLMSSH
460 470 480 490 500
PVVVTVSTPA EITSVFDGIS YSKGASILRM LQDWITPEKF QKGCQIYLEN
510 520 530 540 550
FKFKNAKTSD FWDSLEKASN QPVKEVMDTW TSQMGYPVVT VSGKQNVTQK
560 570 580 590 600
RFLLDYKADP SQPPSALGYT WNIPIKWTEN GNSNITVYYR SNREGITLNA
610 620 630 640 650
NLSGDGFLKI NPDHIGFYRV NYEAETWDWI AETLSSNHMN FSSADRSSFI
660 670 680 690 700
DDAFALARAQ LLDYEKALNL TRYLTSEKDF LPWERVISAV SYIISMFEDD
710 720 730 740 750
RELYPLIETY FRSQVKPIAD SLGWQDTGSH ITKLLRASVL GFACKMGAGE
760 770 780 790 800
ALGNASQLFE AWLKGNESIP VNLRLLVYRY GMQNSGNEAA WNYTLEQYQK
810 820 830 840 850
TSLAQEKEKL LYGLASVKDV TLLARYLEML KDPNIIKTQD VFTVIRYISY
860 870 880 890 900
NSYGKSMAWN WIQLNWDYLV NRFTINDRYL GRIVTIAEPF NTELQLWQMQ
910 920 930 940
SFFAKYPNAG AGAKPREQVL ETVKNNIEWL KLNRKSISEW FTSMP
Length:945
Mass (Da):107,995
Last modified:July 25, 2003 - v2
Checksum:i5A74F1A537DC5937
GO
Isoform 2 (identifier: P50123-2) [UniParc]FASTAAdd to basket
Also known as: APAS

The sequence of this isoform differs from the canonical sequence as follows:
     569-573: YTWNI → NHFEC
     574-945: Missing.

Show »
Length:573
Mass (Da):64,959
Checksum:i74E326B115475340
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256K → E in AAF66704 (PubMed:10978538).Curated1
Sequence conflicti256K → E in AAF66710 (PubMed:10978538).Curated1
Sequence conflicti306N → K in AAF66704 (PubMed:10978538).Curated1
Sequence conflicti306N → K in AAF66710 (PubMed:10978538).Curated1
Sequence conflicti748A → D in AAF66704 (PubMed:10978538).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007844569 – 573YTWNI → NHFEC in isoform 2. 1 Publication5
Alternative sequenceiVSP_007845574 – 945Missing in isoform 2. 1 PublicationAdd BLAST372

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146044 mRNA Translation: AAF66704.1
AF146518 mRNA Translation: AAF66710.1
BC066663 mRNA Translation: AAH66663.1
AF214568 mRNA Translation: AAF37622.1
RefSeqiNP_071587.2, NM_022251.2
UniGeneiRn.162610

Genome annotation databases

GeneIDi64017
KEGGirno:64017
UCSCiRGD:621228 rat [P50123-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAMPE_RAT
AccessioniPrimary (citable) accession number: P50123
Secondary accession number(s): Q64200
, Q9JLQ7, Q9JLQ9, Q9QV24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 25, 2003
Last modified: May 23, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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