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Protein

Glutamyl aminopeptidase

Gene

Enpep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to have a role in the catabolic pathway of the renin-angiotensin system. Isoform 1 has aminopeptidase activity while isoform 2 does not.

Catalytic activityi

Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215SubstrateBy similarity1
Metal bindingi385Zinc; catalyticPROSITE-ProRule annotation1
Active sitei386Proton acceptorPROSITE-ProRule annotation1
Metal bindingi389Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi408Zinc; catalyticPROSITE-ProRule annotation1
Sitei471Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: RGD
  • metalloaminopeptidase activity Source: UniProtKB
  • peptide binding Source: RGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl aminopeptidase (EC:3.4.11.7)
Short name:
EAP
Alternative name(s):
Aminopeptidase A
Short name:
AP-A
CD_antigen: CD249
Gene namesi
Name:Enpep
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621228. Enpep.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini40 – 945ExtracellularSequence analysisAdd BLAST906

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: GO_Central
  • vesicle lumen Source: RGD
  • vesicle membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950981 – 945Glutamyl aminopeptidaseAdd BLAST945

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi116N-linked (GlcNAc...)Sequence analysis1
Glycosylationi189N-linked (GlcNAc...)Sequence analysis1
Glycosylationi236N-linked (GlcNAc...)Sequence analysis1
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1
Glycosylationi546N-linked (GlcNAc...)Sequence analysis1
Glycosylationi584N-linked (GlcNAc...)Sequence analysis1
Glycosylationi601N-linked (GlcNAc...)Sequence analysis1
Glycosylationi640N-linked (GlcNAc...)Sequence analysis1
Glycosylationi669N-linked (GlcNAc...)Sequence analysis1
Glycosylationi754N-linked (GlcNAc...)Sequence analysis1
Glycosylationi766N-linked (GlcNAc...)Sequence analysis1
Glycosylationi792N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP50123.
PRIDEiP50123.

PTM databases

iPTMnetiP50123.
PhosphoSitePlusiP50123.

Expressioni

Tissue specificityi

Highest expression in kidney proximal tubules and ileum enterocytes. High expression also detected in liver and pituitary. Lower levels in heart, adrenal gland and brain. Not detected in aorta, lung or spleen. In heart, higher levels in ventricle than in atrium. Also expressed in glomerular mesangial cells.4 Publications

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP50123. 1 interactor.
STRINGi10116.ENSRNOP00000064188.

Structurei

3D structure databases

ProteinModelPortaliP50123.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 353Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOVERGENiHBG006616.
InParanoidiP50123.
KOiK11141.
PhylomeDBiP50123.

Family and domain databases

InterProiIPR033508. Aminopeptidase_A.
IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF188. PTHR11533:SF188. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P50123-1) [UniParc]FASTAAdd to basket
Also known as: APAL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFAEEEPSK KYCIKGKHVA IICATVVAVG LIVGLSVGLT RSCEPGTTPA
60 70 80 90 100
PSNPPPHTST ALPPQDQNVC PDSDDESGEW KNFRLPDFIQ PVHYDLEVKV
110 120 130 140 150
LMEEDRYTGI VSISVNLSKD TRDLWLHIRE TRITKLPELR RPSGEQVPIR
160 170 180 190 200
RCFEYKKQEY VVIQAEEDLA ATSGDSVYRL TIEFEGWLNG SLVGFYRTTY
210 220 230 240 250
TEDGQTKSIA ATDHEPTDAR KSFPCFDEPN KKATYNISLI HPKEYSALSN
260 270 280 290 300
MPVEKKETLD NDWKKTTFMK SVPMSTYLVC FAVHQFTSIQ RTSRSGKPLT
310 320 330 340 350
VYVQPNQKQT AEYAANITKA VFDFFEDYFA MEYSLPKLDK IAIPDFGTGA
360 370 380 390 400
MENWGLVTYR ETNLLYDPLL SASSNQQRVA SVVAHELVHQ WFGNIVTMDW
410 420 430 440 450
WDDLWLNEGF ASFFEFLGVN HAEADWQMLS QVLLEDVLPV QEDDSLMSSH
460 470 480 490 500
PVVVTVSTPA EITSVFDGIS YSKGASILRM LQDWITPEKF QKGCQIYLEN
510 520 530 540 550
FKFKNAKTSD FWDSLEKASN QPVKEVMDTW TSQMGYPVVT VSGKQNVTQK
560 570 580 590 600
RFLLDYKADP SQPPSALGYT WNIPIKWTEN GNSNITVYYR SNREGITLNA
610 620 630 640 650
NLSGDGFLKI NPDHIGFYRV NYEAETWDWI AETLSSNHMN FSSADRSSFI
660 670 680 690 700
DDAFALARAQ LLDYEKALNL TRYLTSEKDF LPWERVISAV SYIISMFEDD
710 720 730 740 750
RELYPLIETY FRSQVKPIAD SLGWQDTGSH ITKLLRASVL GFACKMGAGE
760 770 780 790 800
ALGNASQLFE AWLKGNESIP VNLRLLVYRY GMQNSGNEAA WNYTLEQYQK
810 820 830 840 850
TSLAQEKEKL LYGLASVKDV TLLARYLEML KDPNIIKTQD VFTVIRYISY
860 870 880 890 900
NSYGKSMAWN WIQLNWDYLV NRFTINDRYL GRIVTIAEPF NTELQLWQMQ
910 920 930 940
SFFAKYPNAG AGAKPREQVL ETVKNNIEWL KLNRKSISEW FTSMP
Length:945
Mass (Da):107,995
Last modified:July 25, 2003 - v2
Checksum:i5A74F1A537DC5937
GO
Isoform 2 (identifier: P50123-2) [UniParc]FASTAAdd to basket
Also known as: APAS

The sequence of this isoform differs from the canonical sequence as follows:
     569-573: YTWNI → NHFEC
     574-945: Missing.

Show »
Length:573
Mass (Da):64,959
Checksum:i74E326B115475340
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256K → E in AAF66704 (PubMed:10978538).Curated1
Sequence conflicti256K → E in AAF66710 (PubMed:10978538).Curated1
Sequence conflicti306N → K in AAF66704 (PubMed:10978538).Curated1
Sequence conflicti306N → K in AAF66710 (PubMed:10978538).Curated1
Sequence conflicti748A → D in AAF66704 (PubMed:10978538).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007844569 – 573YTWNI → NHFEC in isoform 2. 1 Publication5
Alternative sequenceiVSP_007845574 – 945Missing in isoform 2. 1 PublicationAdd BLAST372

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146044 mRNA. Translation: AAF66704.1.
AF146518 mRNA. Translation: AAF66710.1.
BC066663 mRNA. Translation: AAH66663.1.
AF214568 mRNA. Translation: AAF37622.1.
RefSeqiNP_071587.2. NM_022251.2.
UniGeneiRn.162610.

Genome annotation databases

GeneIDi64017.
KEGGirno:64017.
UCSCiRGD:621228. rat. [P50123-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146044 mRNA. Translation: AAF66704.1.
AF146518 mRNA. Translation: AAF66710.1.
BC066663 mRNA. Translation: AAH66663.1.
AF214568 mRNA. Translation: AAF37622.1.
RefSeqiNP_071587.2. NM_022251.2.
UniGeneiRn.162610.

3D structure databases

ProteinModelPortaliP50123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50123. 1 interactor.
STRINGi10116.ENSRNOP00000064188.

Protein family/group databases

MEROPSiM01.003.

PTM databases

iPTMnetiP50123.
PhosphoSitePlusiP50123.

Proteomic databases

PaxDbiP50123.
PRIDEiP50123.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64017.
KEGGirno:64017.
UCSCiRGD:621228. rat. [P50123-1]

Organism-specific databases

CTDi2028.
RGDi621228. Enpep.

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOVERGENiHBG006616.
InParanoidiP50123.
KOiK11141.
PhylomeDBiP50123.

Miscellaneous databases

PROiP50123.

Family and domain databases

InterProiIPR033508. Aminopeptidase_A.
IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF188. PTHR11533:SF188. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPE_RAT
AccessioniPrimary (citable) accession number: P50123
Secondary accession number(s): Q64200
, Q9JLQ7, Q9JLQ9, Q9QV24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 25, 2003
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.