P50120 (RET2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinol-binding protein 2

Alternative name(s):
Cellular retinol-binding protein II
Short name=CRBP-II

Gene names

Name:	RBP2
Synonyms:	CRBP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Intracellular transport of retinol.
Subcellular location	Cytoplasm.
Tissue specificity	Higher expression in adult small intestine and to a much lesser extent in fetal kidney. Ref.5
Domain	Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Sequence similarities	Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Ligand	Retinol-binding Vitamin A
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	epidermis development Traceable author statement PubMed 1654334. Source: ProtInc phototransduction, visible light Traceable author statement. Source: Reactome vitamin A metabolic process Traceable author statement PubMed 2992469. Source: ProtInc
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA cytosol Traceable author statement. Source: Reactome
Molecular_function	retinal binding Inferred from electronic annotation. Source: UniProtKB-KW retinoid binding Traceable author statement Ref.1. Source: ProtInc retinol binding Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 134

133

Retinol-binding protein 2

PRO_0000067395

Sites

Binding site

41

1

Retinoic acid

Binding site

109

1

Retinoic acid By similarity

Experimental info

Sequence conflict

31

1

R → P in AAC50162. Ref.1

Sequence conflict

114

1

D → G in AAH69396. Ref.4

Secondary structure

1

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134

Helix Strand Turn

Details...

Beta strand

7 – 16

10

Helix

17 – 23

7

Helix

28 – 34

7

Beta strand

40 – 46

7

Beta strand

49 – 55

7

Beta strand

60 – 66

7

Beta strand

71 – 74

4

Turn

76 – 79

4

Beta strand

82 – 90

9

Beta strand

93 – 102

10

Beta strand

106 – 112

7

Beta strand

115 – 122

8

Beta strand

125 – 134

10

Sequences

	Sequence		Length	Mass (Da)
	P50120 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A4A7FB157B099A7D	FASTA	134	15,707
	10 20 30 40 50 60 MTRDQNGTWE MESNENFEGY MKALDIDFAT RKIAVRLTQT KVIDQDGDNF KTKTTSTFRN 70 80 90 100 110 120 YDVDFTVGVE FDEYTKSLDN RHVKALVTWE GDVLVCVQKG EKENRGWKQW IEGDKLYLEL 130 TCGDQVCRQV FKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Variation in the expression of cellular retinoid binding proteins in human endometrium throughout the menstrual cycle." Loughney A.D., Kumarendran M.K., Thomas E.J., Redfern C.P.F. Hum. Reprod. 10:1297-1304(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Small intestine.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Identification, retinoid binding and X-ray analysis of a human retinol-binding protein." Folli C., Calderone V., Ottonello S., Bolchi A., Zanotti G., Stoppini M., Berni R. Proc. Natl. Acad. Sci. U.S.A. 98:3710-3715(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Crystal structure of human cellular retinol-binding protein II to 1.2 A resolution." Tarter M., Capaldi S., Carrizo M.E., Ambrosi E., Perduca M., Monaco H.L. Proteins 70:1626-1630(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) IN COMPLEX WITH RETINOL.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U13831 mRNA. Translation: AAC50162.1.
AK291978 mRNA. Translation: BAF84667.1.
CH471052 Genomic DNA. Translation: EAW79036.1.
BC069296 mRNA. Translation: AAH69296.1.
BC069361 mRNA. Translation: AAH69361.1.
BC069396 mRNA. Translation: AAH69396.1.
BC069424 mRNA. Translation: AAH69424.1.
BC069447 mRNA. Translation: AAH69447.1.
BC069513 mRNA. Translation: AAH69513.1.
BC069522 mRNA. Translation: AAH69522.1.

IPI

IPI00419986.

RefSeq

NP_004155.2. NM_004164.2.

UniGene

Hs.655516.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RCQ	X-ray	1.20	A	2-134	[»]
2RCT	X-ray	1.20	A	2-134	[»]
4EDE	X-ray	1.40	A/B	2-134	[»]
4EEJ	X-ray	1.50	A/B	2-134	[»]
4EFG	X-ray	1.58	A/B	2-134	[»]
4EXZ	X-ray	1.61	A/B	2-134	[»]
4GKC	X-ray	1.30	A/B	2-134	[»]

ProteinModelPortal

P50120.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000232217.

PTM databases

PhosphoSite

P50120.

Polymorphism databases

DMDM

62297500.

Proteomic databases

PaxDb

P50120.

PRIDE

P50120.

Protocols and materials databases

DNASU

5948.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000232217; ENSP00000232217; ENSG00000114113.

GeneID

5948.

KEGG

hsa:5948.

UCSC

uc003eth.3. human.

Organism-specific databases

CTD

5948.

GeneCards

GC03M139171.

HGNC

HGNC:9920. RBP2.

HPA

HPA035866.

MIM

180280. gene.

neXtProt

NX_P50120.

PharmGKB

PA34287.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG284832.

HOGENOM

HOG000004831.

HOVERGEN

HBG005633.

InParanoid

P50120.

KO

K14622.

OMA

QNGTWEM.

OrthoDB

EOG4WSWC0.

PhylomeDB

P50120.

Enzyme and pathway databases

BioCyc

MetaCyc:ENSG00000114113-MONOMER.

Reactome

REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

P50120.

Bgee

P50120.

CleanEx

HS_RBP2.

Genevestigator

P50120.

GermOnline

ENSG00000114113. Homo sapiens.

Family and domain databases

Gene3D

2.40.128.20. 1 hit.

InterPro

IPR012674. Calycin.
IPR011038. Calycin-like.
IPR000463. Fatty_acid-bd.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]

Pfam

PF00061. Lipocalin. 1 hit.
[Graphical view]

PRINTS

PR00178. FATTYACIDBP.

SUPFAM

SSF50814. Calycin. 1 hit.

PROSITE

PS00214. FABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P50120.

ChEMBL

CHEMBL2078.

DrugBank

DB00162. Vitamin A.

EvolutionaryTrace

P50120.

GenomeRNAi

5948.

NextBio

23174.

SOURCE

Search...

Entry information

Entry name

RET2_HUMAN

Accession

Primary (citable) accession number: P50120
Secondary accession number(s): A8K7G3, Q6ISQ9, Q6ISS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.