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Protein

Protein S100-B

Gene

S100b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi19 – 321; low affinityAdd BLAST14
Calcium bindingi62 – 732; high affinityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • astrocyte differentiation Source: Ensembl
  • cellular calcium ion homeostasis Source: UniProtKB
  • cellular response to hypoxia Source: Ensembl
  • energy reserve metabolic process Source: UniProtKB
  • learning or memory Source: UniProtKB
  • long-term synaptic potentiation Source: Ensembl
  • memory Source: MGI
  • negative regulation of skeletal muscle cell differentiation Source: Ensembl
  • positive regulation of apoptotic process Source: Ensembl
  • positive regulation of cell proliferation Source: Ensembl
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • regulation of cell shape Source: Ensembl
  • regulation of cytokine biosynthetic process Source: UniProtKB
  • regulation of long-term neuronal synaptic plasticity Source: UniProtKB
  • regulation of neuronal synaptic plasticity Source: MGI
  • response to glucocorticoid Source: Ensembl
  • response to methylmercury Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1251985. Nuclear signaling by ERBB4.
R-MMU-1810476. RIP-mediated NFkB activation via ZBP1.
R-MMU-3134963. DEx/H-box helicases activate type I IFN and inflammatory cytokines production.
R-MMU-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-MMU-879415. Advanced glycosylation endproduct receptor signaling.
R-MMU-933542. TRAF6 mediated NF-kB activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-B
Alternative name(s):
S-100 protein beta chain
S-100 protein subunit beta
S100 calcium-binding protein B
Gene namesi
Name:S100b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:98217. S100b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular region Source: UniProtKB
  • extracellular space Source: Ensembl
  • intracellular membrane-bounded organelle Source: MGI
  • neuronal cell body Source: MGI
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001439672 – 92Protein S100-BAdd BLAST91

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP50114.
PeptideAtlasiP50114.
PRIDEiP50114.

PTM databases

PhosphoSitePlusiP50114.

Expressioni

Tissue specificityi

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Gene expression databases

BgeeiENSMUSG00000033208.
CleanExiMM_S100B.
ExpressionAtlasiP50114. baseline and differential.
GenevisibleiP50114. MM.

Interactioni

Subunit structurei

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with CACYBP in a calcium-dependent manner. Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting. Interacts with S100A6. The S100B dimer interacts with two molecules of CAPZA1. Interacts with AGER. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203058. 1 interactor.
IntActiP50114. 2 interactors.
MINTiMINT-239068.
STRINGi10090.ENSMUSP00000047968.

Structurei

3D structure databases

ProteinModelPortaliP50114.
SMRiP50114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 48EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini49 – 84EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the S-100 family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IYFX. Eukaryota.
ENOG41127J0. LUCA.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP50114.
OMAiCCHEFFE.
OrthoDBiEOG091G13Z2.
PhylomeDBiP50114.
TreeFamiTF332727.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028481. S100-B.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF17. PTHR11639:SF17. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
SM01394. S_100. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE
60 70 80 90
QEVVDKVMET LDEDGDGECD FQEFMAFVAM VTTACHEFFE HE
Length:92
Mass (Da):10,728
Last modified:January 23, 2007 - v2
Checksum:i43815AC212A81DD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22144 Unassigned DNA. Translation: AAA03075.1.
BC061178 mRNA. Translation: AAH61178.1.
CCDSiCCDS35943.1.
PIRiA48015.
RefSeqiNP_033141.1. NM_009115.3.
UniGeneiMm.235998.

Genome annotation databases

EnsembliENSMUST00000036387; ENSMUSP00000047968; ENSMUSG00000033208.
GeneIDi20203.
KEGGimmu:20203.
UCSCiuc007fuc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22144 Unassigned DNA. Translation: AAA03075.1.
BC061178 mRNA. Translation: AAH61178.1.
CCDSiCCDS35943.1.
PIRiA48015.
RefSeqiNP_033141.1. NM_009115.3.
UniGeneiMm.235998.

3D structure databases

ProteinModelPortaliP50114.
SMRiP50114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203058. 1 interactor.
IntActiP50114. 2 interactors.
MINTiMINT-239068.
STRINGi10090.ENSMUSP00000047968.

PTM databases

PhosphoSitePlusiP50114.

Proteomic databases

PaxDbiP50114.
PeptideAtlasiP50114.
PRIDEiP50114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036387; ENSMUSP00000047968; ENSMUSG00000033208.
GeneIDi20203.
KEGGimmu:20203.
UCSCiuc007fuc.2. mouse.

Organism-specific databases

CTDi6285.
MGIiMGI:98217. S100b.

Phylogenomic databases

eggNOGiENOG410IYFX. Eukaryota.
ENOG41127J0. LUCA.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP50114.
OMAiCCHEFFE.
OrthoDBiEOG091G13Z2.
PhylomeDBiP50114.
TreeFamiTF332727.

Enzyme and pathway databases

ReactomeiR-MMU-1251985. Nuclear signaling by ERBB4.
R-MMU-1810476. RIP-mediated NFkB activation via ZBP1.
R-MMU-3134963. DEx/H-box helicases activate type I IFN and inflammatory cytokines production.
R-MMU-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-MMU-879415. Advanced glycosylation endproduct receptor signaling.
R-MMU-933542. TRAF6 mediated NF-kB activation.

Miscellaneous databases

PROiP50114.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033208.
CleanExiMM_S100B.
ExpressionAtlasiP50114. baseline and differential.
GenevisibleiP50114. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028481. S100-B.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF17. PTHR11639:SF17. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 1 hit.
SM01394. S_100. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS100B_MOUSE
AccessioniPrimary (citable) accession number: P50114
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.