Reviewed, UniProtKB/Swiss-Prot P50113 (LYS5_YEAST)

Names and origin

Protein names

Recommended name:
    L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
      Short name=AASD-PPT
    EC=2.7.8.-

Gene names

Name:	LYS5
Ordered Locus Names:	YGL154C
ORF Names:	G1867

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a 4'-phosphopantetheine moiety from coenzyme A to a serine residue of acceptor proteins, such as alpha-aminoadipate reductase. Necessary for alpha-aminoadipate reductase activity. Ref.5
Catalytic activity	CoA-[4'-phosphopantetheine] + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].
Miscellaneous	Present with 623 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.
Biophysicochemical properties	Kinetic parameters: KM=1 µM for CoA KM=1 µM for alpha-aminoadipate reductase PCP fragment

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via aminoadipic acid Traceable author statement. Source: SGD macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro protein-cofactor linkage Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Traceable author statement. Source: SGD
Molecular function	holo-[acyl-carrier-protein] synthase activity Ref.5 Inferred from direct assay. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADE2	P21264	1	EBI-10282,EBI-14252
FZF1	P32805	1	EBI-10282,EBI-7201

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase		PRO_0000175742

Sequences

	Sequence		Length	Mass (Da)
	P50113-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3722252E7EC97787	FASTA	272	30,683
	10 20 30 40 50 60 MVKTTEVVSE VSKVAGVRPW AGIFVVEIQE DILADEFTFE ALMRTLPLAS QARILNKKSF 70 80 90 100 110 120 HDRCSNLCSQ LLQLFGCSIV TGLNFQELKF DKGSFGKPFL DNNRFLPFSM TIGEQYVAMF 130 140 150 160 170 180 LVKCVSTDEY QDVGIDIASP CNYGGREELE LFKEVFSERE FNGLLKASDP CTIFTYLWSL 190 200 210 220 230 240 KESYTKFTGT GLNTDLSLID FGAISFFPAE GASMCITLDE VPLIFHSQWF NNEIVTICMP 250 260 270 KSISDKINTN RPKLYNISLS TLIDYFIEND GL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The LYS5 gene of Saccharomyces cerevisiae." Miller K.G., Bhattacharjee J.K. Gene 172:167-168(1996) [PubMed: 8654981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43." James C.M., Indge K.J., Oliver S.G. Yeast 11:1413-1419(1995) [PubMed: 8585324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5." Ehmann D.E., Gehring A.M., Walsh C.T. Biochemistry 38:6171-6177(1999) [PubMed: 10320345] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U32586 Genomic DNA. Translation: AAC49449.1. Z48618 Genomic DNA. No translation available. Z72676 Genomic DNA. Translation: CAA96866.1. AY692861 Genomic DNA. Translation: AAT92880.1.
PIR	S59762.
RefSeq	NP_011361.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1426N.
IntAct	P50113. 5 interactions.
STRING	P50113.
Genome annotation databases
Ensembl	YGL154C; YGL154C; YGL154C; Saccharomyces cerevisiae. [Genome view]
GeneID	852723.
KEGG	sce:YGL154C.
NMPDR	fig|4932.3.peg.2465.
Organism-specific databases
CYGD	YGL154c.
SGD	S000003122. LYS5.
Phylogenomic databases
HOGENOM	P50113.
OMA	GCSIVTG
OrthoDB	EOG966X39
Gene expression databases
ArrayExpress	P50113.
Genevestigator	P50113.
GermOnline	YGL154C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008278. 4-PPantetheinyl_Trfase. [Graphical view]
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972108.

Entry information

Entry name

LYS5_YEAST

Accession

Primary (citable) accession number: P50113

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)