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Protein

L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase

Gene

LYS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a 4'-phosphopantetheine moiety from coenzyme A to a serine residue of acceptor proteins, such as alpha-aminoadipate reductase. Necessary for alpha-aminoadipate reductase activity.1 Publication

Catalytic activityi

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].

Kineticsi

  1. KM=1 µM for CoA1 Publication
  2. KM=1 µM for alpha-aminoadipate reductase PCP fragment1 Publication

    GO - Molecular functioni

    • holo-[acyl-carrier-protein] synthase activity Source: SGD
    • magnesium ion binding Source: InterPro

    GO - Biological processi

    • lysine biosynthetic process via aminoadipic acid Source: SGD
    • protein-cofactor linkage Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30645-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (EC:2.7.8.7)
    Short name:
    AASD-PPT
    Gene namesi
    Name:LYS5
    Ordered Locus Names:YGL154C
    ORF Names:G1867
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGL154C.
    SGDiS000003122. LYS5.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: SGD
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferasePRO_0000175742Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi33100. 8 interactions.
    DIPiDIP-1426N.
    IntActiP50113. 4 interactions.
    MINTiMINT-410918.

    Structurei

    3D structure databases

    ProteinModelPortaliP50113.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000113423.
    InParanoidiP50113.
    KOiK06133.
    OMAiIASTIDC.
    OrthoDBiEOG7KSXKG.

    Family and domain databases

    Gene3Di3.90.470.20. 2 hits.
    InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
    [Graphical view]
    PfamiPF01648. ACPS. 1 hit.
    [Graphical view]
    SUPFAMiSSF56214. SSF56214. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P50113-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVKTTEVVSE VSKVAGVRPW AGIFVVEIQE DILADEFTFE ALMRTLPLAS
    60 70 80 90 100
    QARILNKKSF HDRCSNLCSQ LLQLFGCSIV TGLNFQELKF DKGSFGKPFL
    110 120 130 140 150
    DNNRFLPFSM TIGEQYVAMF LVKCVSTDEY QDVGIDIASP CNYGGREELE
    160 170 180 190 200
    LFKEVFSERE FNGLLKASDP CTIFTYLWSL KESYTKFTGT GLNTDLSLID
    210 220 230 240 250
    FGAISFFPAE GASMCITLDE VPLIFHSQWF NNEIVTICMP KSISDKINTN
    260 270
    RPKLYNISLS TLIDYFIEND GL
    Length:272
    Mass (Da):30,683
    Last modified:October 1, 1996 - v1
    Checksum:i3722252E7EC97787
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U32586 Genomic DNA. Translation: AAC49449.1.
    Z48618 Genomic DNA. No translation available.
    Z72676 Genomic DNA. Translation: CAA96866.1.
    AY692861 Genomic DNA. Translation: AAT92880.1.
    BK006941 Genomic DNA. Translation: DAA07958.1.
    PIRiS59762.
    RefSeqiNP_011361.1. NM_001181019.1.

    Genome annotation databases

    EnsemblFungiiYGL154C; YGL154C; YGL154C.
    GeneIDi852723.
    KEGGisce:YGL154C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U32586 Genomic DNA. Translation: AAC49449.1.
    Z48618 Genomic DNA. No translation available.
    Z72676 Genomic DNA. Translation: CAA96866.1.
    AY692861 Genomic DNA. Translation: AAT92880.1.
    BK006941 Genomic DNA. Translation: DAA07958.1.
    PIRiS59762.
    RefSeqiNP_011361.1. NM_001181019.1.

    3D structure databases

    ProteinModelPortaliP50113.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi33100. 8 interactions.
    DIPiDIP-1426N.
    IntActiP50113. 4 interactions.
    MINTiMINT-410918.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYGL154C; YGL154C; YGL154C.
    GeneIDi852723.
    KEGGisce:YGL154C.

    Organism-specific databases

    EuPathDBiFungiDB:YGL154C.
    SGDiS000003122. LYS5.

    Phylogenomic databases

    HOGENOMiHOG000113423.
    InParanoidiP50113.
    KOiK06133.
    OMAiIASTIDC.
    OrthoDBiEOG7KSXKG.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30645-MONOMER.

    Miscellaneous databases

    NextBioi972108.
    PROiP50113.

    Family and domain databases

    Gene3Di3.90.470.20. 2 hits.
    InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
    [Graphical view]
    PfamiPF01648. ACPS. 1 hit.
    [Graphical view]
    SUPFAMiSSF56214. SSF56214. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The LYS5 gene of Saccharomyces cerevisiae."
      Miller K.G., Bhattacharjee J.K.
      Gene 172:167-168(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43."
      James C.M., Indge K.J., Oliver S.G.
      Yeast 11:1413-1419(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5."
      Ehmann D.E., Gehring A.M., Walsh C.T.
      Biochemistry 38:6171-6177(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLYS5_YEAST
    AccessioniPrimary (citable) accession number: P50113
    Secondary accession number(s): D6VTZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: May 11, 2016
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 623 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.