Reviewed, UniProtKB/Swiss-Prot P50110 (SAM37_YEAST)

Names and origin

Protein names

Recommended name:
    Sorting assembly machinery 37 kDa subunit
Alternative name(s):
    Mitochondrial 37 kDa outer membrane protein
    MAS37 protein

Gene names

Name:	SAM37
Synonyms:	MAS37, PET3027, TOM37
Ordered Locus Names:	YMR060C
ORF Names:	YM9796.13C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex. Ref.5
Subunit structure	Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50. SAM37 interacts with TOM70.
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1580 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial outer membrane translocase complex assembly Ref.5 Inferred from mutant phenotype. Source: SGD protein import into mitochondrial outer membrane Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial sorting and assembly machinery complex Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Sorting assembly machinery 37 kDa subunit		PRO_0000072630
Regions
	Transmembrane	17 – 33	17	Potential
	Transmembrane	247 – 265	19	Potential

Sequences

	Sequence		Length	Mass (Da)
	P50110-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D4ABA929CE5CAD16	FASTA	327	37,493
	10 20 30 40 50 60 MVKGSVHLWG KDGKASLISV DSIALVWFIK LCTSEEAKSM VAGLQIVFSN NTDLSSDGKL 70 80 90 100 110 120 PVLILDNGTK VSGYVNIVQF LHKNICTSKY EKGTDYEEDL AIVRKKDRLL EYSLLNYVDV 130 140 150 160 170 180 EISRLTDYQL FLNTKNYNEY TKKLFSKLLY FPMWYNTPLQ LRSQARENCE EIIGSLTLED 190 200 210 220 230 240 DEEFVESKAM ESASQLAQSK TFKIAHKNKI KGKQELQQVK YNLQFDNRLQ SCVSNWLAAR 250 260 270 280 290 300 KKLDDSVILS SDLLFLANLY VQLGLPDGNR IRSKLEQTFG SELLNSMSNK IDDFVHRPSN 310 320 NLEQRDPQFR EQGNVVMSLY NLACKYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mas37p, a novel receptor subunit for protein import into mitochondria." Gratzer S., Lithgow T., Bauer R.E., Lamping E., Paltauf F., Kohlwein S.D., Haucke V., Junne T., Schatz G., Horst M. J. Cell Biol. 129:25-34(1995) [PubMed: 7698990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane." Kozjak V., Wiedemann N., Milenkovic D., Lohaus C., Meyer H.E., Guiard B., Meisinger C., Pfanner N. J. Biol. Chem. 278:48520-48523(2003) [PubMed: 14570913] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX.
[5]	"Machinery for protein sorting and assembly in the mitochondrial outer membrane." Wiedemann N., Kozjak V., Chacinska A., Schoenfisch B., Rospert S., Ryan M.T., Pfanner N., Meisinger C. Nature 424:565-571(2003) [PubMed: 12891361] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX, FUNCTION OF THE SAM COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Assembly of the TOB complex of mitochondria." Habib S.J., Waizenegger T., Lech M., Neupert W., Rapaport D. J. Biol. Chem. 280:6434-6440(2005) [PubMed: 15590639] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62565 Genomic DNA. Translation: CAA44438.1. Z49703 Genomic DNA. Translation: CAA89770.1. AY557989 Genomic DNA. Translation: AAS56315.1.
PIR	S54560.
RefSeq	NP_013776.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P50110. 1 interaction.
STRING	P50110.
Protein family/group databases
TCDB	1.B.33.3.1. outer membrane protein insertion porin (OmpIP) family.
Genome annotation databases
Ensembl	YMR060C; YMR060C; YMR060C; Saccharomyces cerevisiae. [Genome view]
GeneID	855082.
GenomeReviews	Gene locus YMR060C in contig Z71257_GR.
KEGG	sce:YMR060C.
NMPDR	fig|4932.3.peg.4823.
Organism-specific databases
CYGD	YMR060c.
SGD	S000004664. SAM37.
Phylogenomic databases
HOGENOM	P50110.
OMA	LAQSKTF.
Gene expression databases
ArrayExpress	P50110.
Genevestigator	P50110.
GermOnline	YMR060C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019564. Mt-OM_transp_Tom37/Metaxin. [Graphical view]
Pfam	PF10568. Tom37. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978369.

Entry information

Entry name

SAM37_YEAST

Accession

Primary (citable) accession number: P50110

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)