P50108 (MNN10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-1,6-mannosyltransferase MNN10
EC=2.4.1.-

Alternative name(s):
Bud emergence delay protein 1
Mannan polymerase II complex MNN10 subunit
Short name=M-Pol II subunit MNN10

Gene names

Name:	MNN10
Synonyms:	BED1
Ordered Locus Names:	YDR245W
ORF Names:	YD8419.12

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for polarized growth and efficient budding. Ref.1 Ref.2 Ref.6 The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. Ref.1 Ref.2 Ref.6
Subunit structure	Component of the M-Pol II complex composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus › cis-Golgi network membrane; Single-pass type II membrane protein Ref.1 Ref.2.
Miscellaneous	Present with 6280 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Traceable author statement Ref.6. Source: SGD barrier septum assembly Inferred from mutant phenotype PubMed 16034811. Source: SGD cell budding Inferred from mutant phenotype Ref.6. Source: UniProtKB cell wall mannoprotein biosynthetic process Inferred from direct assay Ref.6. Source: UniProtKB substituted mannan metabolic process Traceable author statement Ref.6. Source: SGD
Cellular_component	alpha-1,6-mannosyltransferase complex Inferred from direct assay Ref.6. Source: UniProtKB endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-1,6-mannosyltransferase activity Inferred from direct assay PubMed 9430634. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANP1	P32629	3	EBI-11043,EBI-2595
HOC1	P47124	3	EBI-11043,EBI-8430
MNN11	P46985	5	EBI-11043,EBI-11052

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Probable alpha-1,6-mannosyltransferase MNN10		PRO_0000215164
Regions
	Topological domain	1 – 52	52	Cytoplasmic Potential
	Transmembrane	53 – 73	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	74 – 393	320	Lumenal Potential
Experimental info
	Sequence conflict	238	1	N → I in AAC49280. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50108 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FAF7DBE3122ECC8E	FASTA	393	46,748
	10 20 30 40 50 60 MSSVPYNSQL PISNHLEYDE DEKKSRGSKL GLKYKMIYWR KTLCSSLARW RKLILLISLA 70 80 90 100 110 120 LFLFIWISDS TISRNPSTTS FQGQNSNDNK LSNTGSSINS KRYVPPYSKR SRWSFWNQDP 130 140 150 160 170 180 RIVIILAANE GGGVLRWKNE QEWAIEGISI ENKKAYAKRH GYALTIKDLT TSKRYSHEYR 190 200 210 220 230 240 EGWQKVDILR QTFREFPNAE WFWWLDLDTM IMEPSKSLEE HIFDRLETLA DRELKSFNPL 250 260 270 280 290 300 NLRDDIPYVD YSEEMEFLIT QDCGGFNLGS FLIKNSEWSK LLLDMWWDPV LYEQKHMVWE 310 320 330 340 350 360 HREQDALEAL YENEPWIRSR IGFLPLRTIN AFPPGACSEY SGDSRYFYSE KDHDFVVNMA 370 380 390 GCNFGRDCWG EMQYYTTLME KLNRKWYTRF FFP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases." Dean N., Poster J.B. Glycobiology 6:73-81(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae." Mondesert G., Reed S.I. J. Cell Biol. 132:137-151(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: BF264-15DU.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex." Jungmann J., Rayner J.C., Munro S. J. Biol. Chem. 274:6579-6585(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42540 Genomic DNA. Translation: AAB48372.1. U31446 Genomic DNA. Translation: AAC49280.1. Z49701 Genomic DNA. Translation: CAA89731.1. AY557792 Genomic DNA. Translation: AAS56118.1. BK006938 Genomic DNA. Translation: DAA12085.1.
PIR	S54541.
RefSeq	NP_010531.1. NM_001180553.1.
3D structure databases
ProteinModelPortal	P50108.
SMR	P50108. Positions 152-212.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-892N.
IntAct	P50108. 20 interactions.
MINT	MINT-670082.
STRING	4932.YDR245W.
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Proteomic databases
PaxDb	P50108.
PeptideAtlas	P50108.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR245W; YDR245W; YDR245W.
GeneID	851832.
KEGG	sce:YDR245W.
Organism-specific databases
CYGD	YDR245w.
SGD	S000002653. MNN10.
Phylogenomic databases
eggNOG	NOG266323.
HOGENOM	HOG000165792.
KO	K05531.
OMA	HEYREGW.
OrthoDB	EOG441TKR.
Gene expression databases
Genevestigator	P50108.
GermOnline	YDR245W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969721.

Entry information

Entry name

MNN10_YEAST

Accession

Primary (citable) accession number: P50108
Secondary accession number(s): D6VSM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program