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P50108

- MNN10_YEAST

UniProt

P50108 - MNN10_YEAST

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Protein

Probable alpha-1,6-mannosyltransferase MNN10

Gene

MNN10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for polarized growth and efficient budding.
The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins.

GO - Molecular functioni

  1. alpha-1,6-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. barrier septum assembly Source: SGD
  2. cell budding Source: UniProtKB
  3. cell wall mannoprotein biosynthetic process Source: UniProtKB
  4. mannosylation Source: GOC
  5. protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-29818-MONOMER.

Protein family/group databases

CAZyiGT34. Glycosyltransferase Family 34.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-1,6-mannosyltransferase MNN10 (EC:2.4.1.-)
Alternative name(s):
Bud emergence delay protein 1
Mannan polymerase II complex MNN10 subunit
Short name:
M-Pol II subunit MNN10
Gene namesi
Name:MNN10
Synonyms:BED1
Ordered Locus Names:YDR245W
ORF Names:YD8419.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR245w.
SGDiS000002653. MNN10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei53 – 7321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini74 – 393320LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. alpha-1,6-mannosyltransferase complex Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Probable alpha-1,6-mannosyltransferase MNN10PRO_0000215164Add
BLAST

Proteomic databases

MaxQBiP50108.
PaxDbiP50108.
PeptideAtlasiP50108.

Expressioni

Gene expression databases

GenevestigatoriP50108.

Interactioni

Subunit structurei

Component of the M-Pol II complex composed of ANP1, MNN9, MNN10, MNN11 and HOC1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ANP1P326293EBI-11043,EBI-2595
HOC1P471243EBI-11043,EBI-8430
MNN11P469855EBI-11043,EBI-11052

Protein-protein interaction databases

BioGridi32296. 360 interactions.
DIPiDIP-892N.
IntActiP50108. 21 interactions.
MINTiMINT-670082.
STRINGi4932.YDR245W.

Structurei

3D structure databases

ProteinModelPortaliP50108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 34 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG266323.
HOGENOMiHOG000165792.
InParanoidiP50108.
KOiK05531.
OMAiHEYREGW.
OrthoDBiEOG7M3J8X.

Family and domain databases

InterProiIPR008630. Glyco_trans_34.
[Graphical view]
PfamiPF05637. Glyco_transf_34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50108-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSVPYNSQL PISNHLEYDE DEKKSRGSKL GLKYKMIYWR KTLCSSLARW
60 70 80 90 100
RKLILLISLA LFLFIWISDS TISRNPSTTS FQGQNSNDNK LSNTGSSINS
110 120 130 140 150
KRYVPPYSKR SRWSFWNQDP RIVIILAANE GGGVLRWKNE QEWAIEGISI
160 170 180 190 200
ENKKAYAKRH GYALTIKDLT TSKRYSHEYR EGWQKVDILR QTFREFPNAE
210 220 230 240 250
WFWWLDLDTM IMEPSKSLEE HIFDRLETLA DRELKSFNPL NLRDDIPYVD
260 270 280 290 300
YSEEMEFLIT QDCGGFNLGS FLIKNSEWSK LLLDMWWDPV LYEQKHMVWE
310 320 330 340 350
HREQDALEAL YENEPWIRSR IGFLPLRTIN AFPPGACSEY SGDSRYFYSE
360 370 380 390
KDHDFVVNMA GCNFGRDCWG EMQYYTTLME KLNRKWYTRF FFP
Length:393
Mass (Da):46,748
Last modified:October 1, 1996 - v1
Checksum:iFAF7DBE3122ECC8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381N → I in AAC49280. (PubMed:8567719)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42540 Genomic DNA. Translation: AAB48372.1.
U31446 Genomic DNA. Translation: AAC49280.1.
Z49701 Genomic DNA. Translation: CAA89731.1.
AY557792 Genomic DNA. Translation: AAS56118.1.
BK006938 Genomic DNA. Translation: DAA12085.1.
PIRiS54541.
RefSeqiNP_010531.1. NM_001180553.1.

Genome annotation databases

EnsemblFungiiYDR245W; YDR245W; YDR245W.
GeneIDi851832.
KEGGisce:YDR245W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42540 Genomic DNA. Translation: AAB48372.1 .
U31446 Genomic DNA. Translation: AAC49280.1 .
Z49701 Genomic DNA. Translation: CAA89731.1 .
AY557792 Genomic DNA. Translation: AAS56118.1 .
BK006938 Genomic DNA. Translation: DAA12085.1 .
PIRi S54541.
RefSeqi NP_010531.1. NM_001180553.1.

3D structure databases

ProteinModelPortali P50108.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32296. 360 interactions.
DIPi DIP-892N.
IntActi P50108. 21 interactions.
MINTi MINT-670082.
STRINGi 4932.YDR245W.

Protein family/group databases

CAZyi GT34. Glycosyltransferase Family 34.

Proteomic databases

MaxQBi P50108.
PaxDbi P50108.
PeptideAtlasi P50108.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR245W ; YDR245W ; YDR245W .
GeneIDi 851832.
KEGGi sce:YDR245W.

Organism-specific databases

CYGDi YDR245w.
SGDi S000002653. MNN10.

Phylogenomic databases

eggNOGi NOG266323.
HOGENOMi HOG000165792.
InParanoidi P50108.
KOi K05531.
OMAi HEYREGW.
OrthoDBi EOG7M3J8X.

Enzyme and pathway databases

BioCyci YEAST:G3O-29818-MONOMER.

Miscellaneous databases

NextBioi 969721.

Gene expression databases

Genevestigatori P50108.

Family and domain databases

InterProi IPR008630. Glyco_trans_34.
[Graphical view ]
Pfami PF05637. Glyco_transf_34. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases."
    Dean N., Poster J.B.
    Glycobiology 6:73-81(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae."
    Mondesert G., Reed S.I.
    J. Cell Biol. 132:137-151(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: BF264-15DU.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex."
    Jungmann J., Rayner J.C., Munro S.
    J. Biol. Chem. 274:6579-6585(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMNN10_YEAST
AccessioniPrimary (citable) accession number: P50108
Secondary accession number(s): D6VSM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3