Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P50108 (MNN10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alpha-1,6-mannosyltransferase MNN10

EC=2.4.1.-
Alternative name(s):
Bud emergence delay protein 1
Mannan polymerase II complex MNN10 subunit
Short name=M-Pol II subunit MNN10
Gene names
Name:MNN10
Synonyms:BED1
Ordered Locus Names:YDR245W
ORF Names:YD8419.12
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for polarized growth and efficient budding. Ref.1 Ref.2 Ref.6

The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. Ref.1 Ref.2 Ref.6

Subunit structure

Component of the M-Pol II complex composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatuscis-Golgi network membrane; Single-pass type II membrane protein Ref.1 Ref.2.

Miscellaneous

Present with 6280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase 34 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Probable alpha-1,6-mannosyltransferase MNN10
PRO_0000215164

Regions

Topological domain1 – 5252Cytoplasmic Potential
Transmembrane53 – 7321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain74 – 393320Lumenal Potential

Experimental info

Sequence conflict2381N → I in AAC49280. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P50108 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: FAF7DBE3122ECC8E

FASTA39346,748
        10         20         30         40         50         60 
MSSVPYNSQL PISNHLEYDE DEKKSRGSKL GLKYKMIYWR KTLCSSLARW RKLILLISLA 

        70         80         90        100        110        120 
LFLFIWISDS TISRNPSTTS FQGQNSNDNK LSNTGSSINS KRYVPPYSKR SRWSFWNQDP 

       130        140        150        160        170        180 
RIVIILAANE GGGVLRWKNE QEWAIEGISI ENKKAYAKRH GYALTIKDLT TSKRYSHEYR 

       190        200        210        220        230        240 
EGWQKVDILR QTFREFPNAE WFWWLDLDTM IMEPSKSLEE HIFDRLETLA DRELKSFNPL 

       250        260        270        280        290        300 
NLRDDIPYVD YSEEMEFLIT QDCGGFNLGS FLIKNSEWSK LLLDMWWDPV LYEQKHMVWE 

       310        320        330        340        350        360 
HREQDALEAL YENEPWIRSR IGFLPLRTIN AFPPGACSEY SGDSRYFYSE KDHDFVVNMA 

       370        380        390 
GCNFGRDCWG EMQYYTTLME KLNRKWYTRF FFP 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases."
Dean N., Poster J.B.
Glycobiology 6:73-81(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae."
Mondesert G., Reed S.I.
J. Cell Biol. 132:137-151(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: BF264-15DU.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex."
Jungmann J., Rayner J.C., Munro S.
J. Biol. Chem. 274:6579-6585(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42540 Genomic DNA. Translation: AAB48372.1.
U31446 Genomic DNA. Translation: AAC49280.1.
Z49701 Genomic DNA. Translation: CAA89731.1.
AY557792 Genomic DNA. Translation: AAS56118.1.
BK006938 Genomic DNA. Translation: DAA12085.1.
PIRS54541.
RefSeqNP_010531.1. NM_001180553.1.

3D structure databases

ProteinModelPortalP50108.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32296. 359 interactions.
DIPDIP-892N.
IntActP50108. 21 interactions.
MINTMINT-670082.
STRING4932.YDR245W.

Protein family/group databases

CAZyGT34. Glycosyltransferase Family 34.

Proteomic databases

PaxDbP50108.
PeptideAtlasP50108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR245W; YDR245W; YDR245W.
GeneID851832.
KEGGsce:YDR245W.

Organism-specific databases

CYGDYDR245w.
SGDS000002653. MNN10.

Phylogenomic databases

eggNOGNOG266323.
HOGENOMHOG000165792.
KOK05531.
OMAHEYREGW.
OrthoDBEOG7M3J8X.

Enzyme and pathway databases

BioCycYEAST:G3O-29818-MONOMER.

Gene expression databases

GenevestigatorP50108.

Family and domain databases

InterProIPR008630. Glyco_trans_34.
[Graphical view]
PfamPF05637. Glyco_transf_34. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969721.

Entry information

Entry nameMNN10_YEAST
AccessionPrimary (citable) accession number: P50108
Secondary accession number(s): D6VSM5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families