Reviewed, UniProtKB/Swiss-Prot P50108 (MNN10_YEAST)

Names and origin

Protein names

Recommended name:
    Probable alpha-1,6-mannosyltransferase MNN10
    EC=2.4.1.-
Alternative name(s):
    Mannan polymerase II complex MNN10 subunit
      Short name=M-Pol II subunit MNN10
    Bud emergence delay protein 1

Gene names

Name:	MNN10
Synonyms:	BED1
Ordered Locus Names:	YDR245W
ORF Names:	YD8419.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for polarized growth and efficient budding. Ref.1 Ref.2 Ref.5 The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. Ref.1 Ref.2 Ref.5
Subunit structure	Component of the M-Pol II complex composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus › cis-Golgi network membrane; Single-pass type II membrane protein. Ref.1 Ref.2
Miscellaneous	Present with 6280 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-glycan processing Ref.5 Traceable author statement. Source: SGD barrier septum formation Inferred from mutant phenotype. Source: SGD cell budding Ref.5 Inferred from mutant phenotype. Source: UniProtKB cell wall mannoprotein biosynthetic process Ref.5 Inferred from direct assay. Source: UniProtKB substituted mannan metabolic process Ref.5 Traceable author statement. Source: SGD
Cellular component	alpha-1,6-mannosyltransferase complex Ref.5 Inferred from direct assay. Source: UniProtKB endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosyltransferase activity Ref.5 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Probable alpha-1,6-mannosyltransferase MNN10		PRO_0000215164
Regions
	Topological domain	1 – 52	52	Cytoplasmic Potential
	Transmembrane	53 – 73	21	Signal-anchor for type II membrane protein Potential
	Topological domain	74 – 393	320	Lumenal Potential
Experimental info
	Sequence conflict	238	1	N → I in AAC49280. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50108-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FAF7DBE3122ECC8E	FASTA	393	46,748
	10 20 30 40 50 60 MSSVPYNSQL PISNHLEYDE DEKKSRGSKL GLKYKMIYWR KTLCSSLARW RKLILLISLA 70 80 90 100 110 120 LFLFIWISDS TISRNPSTTS FQGQNSNDNK LSNTGSSINS KRYVPPYSKR SRWSFWNQDP 130 140 150 160 170 180 RIVIILAANE GGGVLRWKNE QEWAIEGISI ENKKAYAKRH GYALTIKDLT TSKRYSHEYR 190 200 210 220 230 240 EGWQKVDILR QTFREFPNAE WFWWLDLDTM IMEPSKSLEE HIFDRLETLA DRELKSFNPL 250 260 270 280 290 300 NLRDDIPYVD YSEEMEFLIT QDCGGFNLGS FLIKNSEWSK LLLDMWWDPV LYEQKHMVWE 310 320 330 340 350 360 HREQDALEAL YENEPWIRSR IGFLPLRTIN AFPPGACSEY SGDSRYFYSE KDHDFVVNMA 370 380 390 GCNFGRDCWG EMQYYTTLME KLNRKWYTRF FFP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases." Dean N., Poster J.B. Glycobiology 6:73-81(1996) [PubMed: 8991513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae." Mondesert G., Reed S.I. J. Cell Biol. 132:137-151(1996) [PubMed: 8567719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: BF264-15DU.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex." Jungmann J., Rayner J.C., Munro S. J. Biol. Chem. 274:6579-6585(1999) [PubMed: 10037752] [Abstract] Cited for: FUNCTION, SUBUNIT.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42540 Genomic DNA. Translation: AAB48372.1. U31446 Genomic DNA. Translation: AAC49280.1. Z49701 Genomic DNA. Translation: CAA89731.1. AY557792 Genomic DNA. Translation: AAS56118.1.
PIR	S54541.
RefSeq	NP_010531.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:892N.
IntAct	P50108. 23 interactions.
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Proteomic databases
PeptideAtlas	P50108.
PRIDE	P50108.
Genome annotation databases
Ensembl	YDR245W. Saccharomyces cerevisiae. [Contig view]
GeneID	851832.
GenomeReviews	Gene locus YDR245W in contig Z71256_GR.
KEGG	sce:YDR245W.
NMPDR	fig|4932.3.peg.1288.
Organism-specific databases
CYGD	YDR245w.
SGD	S000002653. MNN10.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P50108.
OMA	P50108. HEYREGW.
Gene expression databases
GermOnline	YDR245W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969721.

Entry information

Entry name

MNN10_YEAST

Accession

Primary (citable) accession number: P50108

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)