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P50107

- LGUL_YEAST

UniProt

P50107 - LGUL_YEAST

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Protein

Lactoylglutathione lyase

Gene
GLO1, YML004C, YM9571.15C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.2 Publications

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi25 – 251Zinc; via tele nitrogen; shared with dimeric partner By similarity
Binding sitei29 – 291Substrate By similarity
Metal bindingi89 – 891Zinc; shared with dimeric partner By similarity
Binding sitei93 – 931Substrate By similarity
Binding sitei113 – 1131Substrate By similarity
Metal bindingi117 – 1171Zinc; via tele nitrogen By similarity
Binding sitei117 – 1171Substrate By similarity
Active sitei163 – 1631Proton donor/acceptor By similarity
Metal bindingi163 – 1631Zinc By similarity

GO - Molecular functioni

  1. lactoylglutathione lyase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glutathione metabolic process Source: SGD
  2. methylglyoxal catabolic process to D-lactate Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-624.
YEAST:YML004C-MONOMER.
SABIO-RKP50107.
UniPathwayiUPA00619; UER00675.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:GLO1
Ordered Locus Names:YML004C
ORF Names:YM9571.15C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML004c.
SGDiS000004463. GLO1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Lactoylglutathione lyasePRO_0000168087Add
BLAST

Proteomic databases

MaxQBiP50107.
PaxDbiP50107.
PeptideAtlasiP50107.

Expressioni

Gene expression databases

GenevestigatoriP50107.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi35167. 11 interactions.
MINTiMINT-4496565.

Structurei

3D structure databases

ProteinModelPortaliP50107.
SMRiP50107. Positions 18-165, 183-326.

Family & Domainsi

Sequence similaritiesi

Belongs to the glyoxalase I family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0346.
GeneTreeiENSGT00390000009312.
HOGENOMiHOG000215632.
KOiK01759.
OMAiTEGRMKN.
OrthoDBiEOG7HXD33.

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR025870. Glyoxalase-like_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
PF12681. Glyoxalase_2. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 2 hits.
TIGRFAMsiTIGR00068. glyox_I. 2 hits.
PROSITEiPS00934. GLYOXALASE_I_1. 2 hits.
PS00935. GLYOXALASE_I_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50107-1 [UniParc]FASTAAdd to Basket

« Hide

MSTDSTRYPI QIEKASNDPT LLLNHTCLRV KDPARTVKFY TEHFGMKLLS    50
RKDFEEAKFS LYFLSFPKDD IPKNKNGEPD VFSAHGVLEL THNWGTEKNP 100
DYKINNGNEE PHRGFGHICF SVSDINKTCE ELESQGVKFK KRLSEGRQKD 150
IAFALGPDGY WIELITYSRE GQEYPKGSVG NKFNHTMIRI KNPTRSLEFY 200
QNVLGMKLLR TSEHESAKFT LYFLGYGVPK TDSVFSCESV LELTHNWGTE 250
NDPNFHYHNG NSEPQGYGHI CISCDDAGAL CKEIEVKYGD KIQWSPKFNQ 300
GRMKNIAFLK DPDGYSIEVV PHGLIA 326
Length:326
Mass (Da):37,209
Last modified:October 1, 1996 - v1
Checksum:i9726A8253F53FB2B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361T → A in CAC16163. 1 Publication
Sequence conflicti156 – 1561G → D in CAC16163. 1 Publication
Sequence conflicti156 – 1561G → D AA sequence 1 Publication
Sequence conflicti322 – 3221H → Y in CAC16163. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99240 Genomic DNA. Translation: CAA67622.1.
AJ297938 Genomic DNA. Translation: CAC16163.1.
Z49810 Genomic DNA. Translation: CAA89948.1.
S80483 Genomic DNA. Translation: AAB21302.1.
BK006946 Genomic DNA. Translation: DAA09895.1.
PIRiS55115.
RefSeqiNP_013710.1. NM_001182359.1.

Genome annotation databases

EnsemblFungiiYML004C; YML004C; YML004C.
GeneIDi855009.
KEGGisce:YML004C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99240 Genomic DNA. Translation: CAA67622.1 .
AJ297938 Genomic DNA. Translation: CAC16163.1 .
Z49810 Genomic DNA. Translation: CAA89948.1 .
S80483 Genomic DNA. Translation: AAB21302.1 .
BK006946 Genomic DNA. Translation: DAA09895.1 .
PIRi S55115.
RefSeqi NP_013710.1. NM_001182359.1.

3D structure databases

ProteinModelPortali P50107.
SMRi P50107. Positions 18-165, 183-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35167. 11 interactions.
MINTi MINT-4496565.

Chemistry

BindingDBi P50107.
ChEMBLi CHEMBL6057.

Proteomic databases

MaxQBi P50107.
PaxDbi P50107.
PeptideAtlasi P50107.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML004C ; YML004C ; YML004C .
GeneIDi 855009.
KEGGi sce:YML004C.

Organism-specific databases

CYGDi YML004c.
SGDi S000004463. GLO1.

Phylogenomic databases

eggNOGi COG0346.
GeneTreei ENSGT00390000009312.
HOGENOMi HOG000215632.
KOi K01759.
OMAi TEGRMKN.
OrthoDBi EOG7HXD33.

Enzyme and pathway databases

UniPathwayi UPA00619 ; UER00675 .
BioCyci MetaCyc:MONOMER-624.
YEAST:YML004C-MONOMER.
SABIO-RK P50107.

Miscellaneous databases

NextBioi 978174.
PROi P50107.

Gene expression databases

Genevestigatori P50107.

Family and domain databases

Gene3Di 3.10.180.10. 2 hits.
InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR025870. Glyoxalase-like_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view ]
Pfami PF00903. Glyoxalase. 1 hit.
PF12681. Glyoxalase_2. 1 hit.
[Graphical view ]
SUPFAMi SSF54593. SSF54593. 2 hits.
TIGRFAMsi TIGR00068. glyox_I. 2 hits.
PROSITEi PS00934. GLYOXALASE_I_1. 2 hits.
PS00935. GLYOXALASE_I_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the structural gene for glyoxalase I from Saccharomyces cerevisiae."
    Inoue Y., Kimura A.
    J. Biol. Chem. 271:25958-25965(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "Yeast glyoxalase I is a monomeric enzyme with two active sites."
    Frickel E.M., Jemth P., Widersten M., Mannervik B.
    J. Biol. Chem. 276:1845-1849(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Positive effect of GAC gene product on the mRNA level of glyoxalase I gene in Saccharomyces cerevisiae."
    Inoue Y., Yano H., Ginya H., Tsuchiyama H., Murata K., Kimura A.
    Biotechnol. Appl. Biochem. 14:391-394(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 310-326, PROTEIN SEQUENCE OF 39-45; 150-165 AND 311-323.
    Strain: ATCC 26109 / X2180.
  6. "The primary structure of monomeric yeast glyoxalase I indicates a gene duplication resulting in two similar segments homologous with the subunit of dimeric human glyoxalase I."
    Ridderstroem M., Mannervik B.
    Biochem. J. 316:1005-1006(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLGUL_YEAST
AccessioniPrimary (citable) accession number: P50107
Secondary accession number(s): D6VZH1, Q9HFG0, Q9URB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2570 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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