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Protein

Lactoylglutathione lyase

Gene

GLO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.2 Publications

Miscellaneous

Present with 2570 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 1 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLO1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (GLO4), Hydroxyacylglutathione hydrolase, cytoplasmic isozyme (GLO2)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi25Zinc; via tele nitrogen; shared with dimeric partnerBy similarity1
Binding sitei29SubstrateBy similarity1
Metal bindingi89Zinc; shared with dimeric partnerBy similarity1
Binding sitei93SubstrateBy similarity1
Binding sitei113SubstrateBy similarity1
Metal bindingi117Zinc; via tele nitrogenBy similarity1
Binding sitei117SubstrateBy similarity1
Active sitei163Proton donor/acceptorBy similarity1
Metal bindingi163ZincBy similarity1

GO - Molecular functioni

  • lactoylglutathione lyase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • glutathione metabolic process Source: SGD
  • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: SGD

Keywordsi

Molecular functionLyase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YML004C-MONOMER
YEAST:YML004C-MONOMER
ReactomeiR-SCE-70268 Pyruvate metabolism
SABIO-RKiP50107
UniPathwayiUPA00619; UER00675

Names & Taxonomyi

Protein namesi
Recommended name:
Lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:GLO1
Ordered Locus Names:YML004C
ORF Names:YM9571.15C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML004C
SGDiS000004463 GLO1

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6057

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001680871 – 326Lactoylglutathione lyaseAdd BLAST326

Proteomic databases

MaxQBiP50107
PaxDbiP50107
PRIDEiP50107

PTM databases

iPTMnetiP50107

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi35167, 46 interactors
STRINGi4932.YML004C

Chemistry databases

BindingDBiP50107

Structurei

3D structure databases

ProteinModelPortaliP50107
SMRiP50107
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 167VOC 1PROSITE-ProRule annotationAdd BLAST146
Domaini182 – 322VOC 2PROSITE-ProRule annotationAdd BLAST141

Sequence similaritiesi

Belongs to the glyoxalase I family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000009312
HOGENOMiHOG000215632
InParanoidiP50107
KOiK01759
OMAiMGDAWGH
OrthoDBiEOG092C5AJ6

Family and domain databases

Gene3Di3.10.180.10, 2 hits
InterProiView protein in InterPro
IPR029068 Glyas_Bleomycin-R_OHBP_Dase
IPR004360 Glyas_Fos-R_dOase_dom
IPR004361 Glyoxalase_1
IPR018146 Glyoxalase_1_CS
IPR037523 VOC
PfamiView protein in Pfam
PF00903 Glyoxalase, 2 hits
SUPFAMiSSF54593 SSF54593, 2 hits
TIGRFAMsiTIGR00068 glyox_I, 2 hits
PROSITEiView protein in PROSITE
PS00934 GLYOXALASE_I_1, 2 hits
PS00935 GLYOXALASE_I_2, 2 hits
PS51819 VOC, 2 hits

Sequencei

Sequence statusi: Complete.

P50107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDSTRYPI QIEKASNDPT LLLNHTCLRV KDPARTVKFY TEHFGMKLLS
60 70 80 90 100
RKDFEEAKFS LYFLSFPKDD IPKNKNGEPD VFSAHGVLEL THNWGTEKNP
110 120 130 140 150
DYKINNGNEE PHRGFGHICF SVSDINKTCE ELESQGVKFK KRLSEGRQKD
160 170 180 190 200
IAFALGPDGY WIELITYSRE GQEYPKGSVG NKFNHTMIRI KNPTRSLEFY
210 220 230 240 250
QNVLGMKLLR TSEHESAKFT LYFLGYGVPK TDSVFSCESV LELTHNWGTE
260 270 280 290 300
NDPNFHYHNG NSEPQGYGHI CISCDDAGAL CKEIEVKYGD KIQWSPKFNQ
310 320
GRMKNIAFLK DPDGYSIEVV PHGLIA
Length:326
Mass (Da):37,209
Last modified:October 1, 1996 - v1
Checksum:i9726A8253F53FB2B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36T → A in CAC16163 (PubMed:11050082).Curated1
Sequence conflicti156G → D in CAC16163 (PubMed:11050082).Curated1
Sequence conflicti156G → D AA sequence (PubMed:1777125).Curated1
Sequence conflicti322H → Y in CAC16163 (PubMed:11050082).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99240 Genomic DNA Translation: CAA67622.1
AJ297938 Genomic DNA Translation: CAC16163.1
Z49810 Genomic DNA Translation: CAA89948.1
S80483 Genomic DNA Translation: AAB21302.1
BK006946 Genomic DNA Translation: DAA09895.1
PIRiS55115
RefSeqiNP_013710.1, NM_001182359.1

Genome annotation databases

EnsemblFungiiYML004C; YML004C; YML004C
GeneIDi855009
KEGGisce:YML004C

Similar proteinsi

Entry informationi

Entry nameiLGUL_YEAST
AccessioniPrimary (citable) accession number: P50107
Secondary accession number(s): D6VZH1, Q9HFG0, Q9URB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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