Reviewed, UniProtKB/Swiss-Prot P50107 (LGUL_YEAST)

Names and origin

Protein names

Recommended name:
    Lactoylglutathione lyase
    EC=4.4.1.5
Alternative name(s):
    Methylglyoxalase
    Aldoketomutase
    Glyoxalase I
      Short name=Glx I
    Ketone-aldehyde mutase
    S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	GLO1
Ordered Locus Names:	YML004C
ORF Names:	YM9571.15C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Ref.1 Ref.2
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Subunit structure	Monomer.
Miscellaneous	Present with 2570 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glutathione metabolic process Ref.1 Inferred from genetic interaction. Source: SGD methylglyoxal catabolic process to D-lactate Ref.1 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	lactoylglutathione lyase activity Ref.1 Inferred from genetic interaction. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Lactoylglutathione lyase		PRO_0000168087
Sites
	Metal binding	242	1	Zinc Potential
	Metal binding	245	1	Zinc Potential
	Metal binding	258	1	Zinc Potential
	Metal binding	269	1	Zinc Potential
Experimental info
	Sequence conflict	36	1	T → A in CAC16163. Ref.2
	Sequence conflict	156	1	G → D in CAC16163. Ref.2
	Sequence conflict	156	1	G → D AA sequence Ref.4
	Sequence conflict	322	1	H → Y in CAC16163. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50107-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9726A8253F53FB2B	FASTA	326	37,209
	10 20 30 40 50 60 MSTDSTRYPI QIEKASNDPT LLLNHTCLRV KDPARTVKFY TEHFGMKLLS RKDFEEAKFS 70 80 90 100 110 120 LYFLSFPKDD IPKNKNGEPD VFSAHGVLEL THNWGTEKNP DYKINNGNEE PHRGFGHICF 130 140 150 160 170 180 SVSDINKTCE ELESQGVKFK KRLSEGRQKD IAFALGPDGY WIELITYSRE GQEYPKGSVG 190 200 210 220 230 240 NKFNHTMIRI KNPTRSLEFY QNVLGMKLLR TSEHESAKFT LYFLGYGVPK TDSVFSCESV 250 260 270 280 290 300 LELTHNWGTE NDPNFHYHNG NSEPQGYGHI CISCDDAGAL CKEIEVKYGD KIQWSPKFNQ 310 320 GRMKNIAFLK DPDGYSIEVV PHGLIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the structural gene for glyoxalase I from Saccharomyces cerevisiae." Inoue Y., Kimura A. J. Biol. Chem. 271:25958-25965(1996) [PubMed: 8824231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204508 / S288c.
[2]	"Yeast glyoxalase I is a monomeric enzyme with two active sites." Frickel E.M., Jemth P., Widersten M., Mannervik B. J. Biol. Chem. 276:1845-1849(2001) [PubMed: 11050082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Positive effect of GAC gene product on the mRNA level of glyoxalase I gene in Saccharomyces cerevisiae." Inoue Y., Yano H., Ginya H., Tsuchiyama H., Murata K., Kimura A. Biotechnol. Appl. Biochem. 14:391-394(1991) [PubMed: 1777125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 310-326, PROTEIN SEQUENCE OF 39-45; 150-165 AND 311-323. Strain: ATCC 26109 / X2180.
[5]	"The primary structure of monomeric yeast glyoxalase I indicates a gene duplication resulting in two similar segments homologous with the subunit of dimeric human glyoxalase I." Ridderstroem M., Mannervik B. Biochem. J. 316:1005-1006(1996) [PubMed: 8670139] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99240 Genomic DNA. Translation: CAA67622.1. AJ297938 Genomic DNA. Translation: CAC16163.1. Z49810 Genomic DNA. Translation: CAA89948.1. S80483 Genomic DNA. Translation: AAB21302.1.
PIR	S55115.
RefSeq	NP_013710.1.
3D structure databases
HSSP	HSSP built from PDB template 1QIP based on UniProtKB Q04760.
ModBase	Search...
Protein-protein interaction databases
IntAct	P50107. 1 interaction.
STRING	P50107.
Proteomic databases
PeptideAtlas	P50107.
Genome annotation databases
Ensembl	YML004C; YML004C; YML004C; Saccharomyces cerevisiae. [Genome view]
GeneID	855009.
GenomeReviews	Gene locus YML004C in contig Z71257_GR.
KEGG	sce:YML004C.
NMPDR	fig|4932.3.peg.4753.
Organism-specific databases
CYGD	YML004c.
SGD	S000004463. GLO1.
Phylogenomic databases
HOGENOM	P50107.
OMA	PHRGFGH.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-624.
BRENDA	4.4.1.5. 250.
Gene expression databases
ArrayExpress	P50107.
Genevestigator	P50107.
GermOnline	YML004C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00068. glyox_I. 2 hits.
PROSITE	PS00934. GLYOXALASE_I_1. 2 hits. PS00935. GLYOXALASE_I_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978174.

Entry information

Entry name

LGUL_YEAST

Accession

Primary (citable) accession number: P50107
Secondary accession number(s): Q9HFG0, Q9URB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)