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P50107

- LGUL_YEAST

UniProt

P50107 - LGUL_YEAST

Protein

Lactoylglutathione lyase

Gene

GLO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.2 Publications

    Catalytic activityi

    (R)-S-lactoylglutathione = glutathione + methylglyoxal.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi25 – 251Zinc; via tele nitrogen; shared with dimeric partnerBy similarity
    Binding sitei29 – 291SubstrateBy similarity
    Metal bindingi89 – 891Zinc; shared with dimeric partnerBy similarity
    Binding sitei93 – 931SubstrateBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Metal bindingi117 – 1171Zinc; via tele nitrogenBy similarity
    Binding sitei117 – 1171SubstrateBy similarity
    Active sitei163 – 1631Proton donor/acceptorBy similarity
    Metal bindingi163 – 1631ZincBy similarity

    GO - Molecular functioni

    1. lactoylglutathione lyase activity Source: SGD
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutathione metabolic process Source: SGD
    2. methylglyoxal catabolic process to D-lactate Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-624.
    YEAST:YML004C-MONOMER.
    SABIO-RKP50107.
    UniPathwayiUPA00619; UER00675.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactoylglutathione lyase (EC:4.4.1.5)
    Alternative name(s):
    Aldoketomutase
    Glyoxalase I
    Short name:
    Glx I
    Ketone-aldehyde mutase
    Methylglyoxalase
    S-D-lactoylglutathione methylglyoxal lyase
    Gene namesi
    Name:GLO1
    Ordered Locus Names:YML004C
    ORF Names:YM9571.15C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML004c.
    SGDiS000004463. GLO1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Lactoylglutathione lyasePRO_0000168087Add
    BLAST

    Proteomic databases

    MaxQBiP50107.
    PaxDbiP50107.
    PeptideAtlasiP50107.

    Expressioni

    Gene expression databases

    GenevestigatoriP50107.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi35167. 11 interactions.
    MINTiMINT-4496565.

    Structurei

    3D structure databases

    ProteinModelPortaliP50107.
    SMRiP50107. Positions 18-165, 183-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glyoxalase I family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0346.
    GeneTreeiENSGT00390000009312.
    HOGENOMiHOG000215632.
    KOiK01759.
    OMAiTEGRMKN.
    OrthoDBiEOG7HXD33.

    Family and domain databases

    Gene3Di3.10.180.10. 2 hits.
    InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR004360. Glyas_Fos-R_dOase_dom.
    IPR025870. Glyoxalase-like_dom.
    IPR004361. Glyoxalase_1.
    IPR018146. Glyoxalase_1_CS.
    [Graphical view]
    PfamiPF00903. Glyoxalase. 1 hit.
    PF12681. Glyoxalase_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54593. SSF54593. 2 hits.
    TIGRFAMsiTIGR00068. glyox_I. 2 hits.
    PROSITEiPS00934. GLYOXALASE_I_1. 2 hits.
    PS00935. GLYOXALASE_I_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50107-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTDSTRYPI QIEKASNDPT LLLNHTCLRV KDPARTVKFY TEHFGMKLLS    50
    RKDFEEAKFS LYFLSFPKDD IPKNKNGEPD VFSAHGVLEL THNWGTEKNP 100
    DYKINNGNEE PHRGFGHICF SVSDINKTCE ELESQGVKFK KRLSEGRQKD 150
    IAFALGPDGY WIELITYSRE GQEYPKGSVG NKFNHTMIRI KNPTRSLEFY 200
    QNVLGMKLLR TSEHESAKFT LYFLGYGVPK TDSVFSCESV LELTHNWGTE 250
    NDPNFHYHNG NSEPQGYGHI CISCDDAGAL CKEIEVKYGD KIQWSPKFNQ 300
    GRMKNIAFLK DPDGYSIEVV PHGLIA 326
    Length:326
    Mass (Da):37,209
    Last modified:October 1, 1996 - v1
    Checksum:i9726A8253F53FB2B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361T → A in CAC16163. (PubMed:11050082)Curated
    Sequence conflicti156 – 1561G → D in CAC16163. (PubMed:11050082)Curated
    Sequence conflicti156 – 1561G → D AA sequence (PubMed:1777125)Curated
    Sequence conflicti322 – 3221H → Y in CAC16163. (PubMed:11050082)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99240 Genomic DNA. Translation: CAA67622.1.
    AJ297938 Genomic DNA. Translation: CAC16163.1.
    Z49810 Genomic DNA. Translation: CAA89948.1.
    S80483 Genomic DNA. Translation: AAB21302.1.
    BK006946 Genomic DNA. Translation: DAA09895.1.
    PIRiS55115.
    RefSeqiNP_013710.1. NM_001182359.1.

    Genome annotation databases

    EnsemblFungiiYML004C; YML004C; YML004C.
    GeneIDi855009.
    KEGGisce:YML004C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99240 Genomic DNA. Translation: CAA67622.1 .
    AJ297938 Genomic DNA. Translation: CAC16163.1 .
    Z49810 Genomic DNA. Translation: CAA89948.1 .
    S80483 Genomic DNA. Translation: AAB21302.1 .
    BK006946 Genomic DNA. Translation: DAA09895.1 .
    PIRi S55115.
    RefSeqi NP_013710.1. NM_001182359.1.

    3D structure databases

    ProteinModelPortali P50107.
    SMRi P50107. Positions 18-165, 183-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35167. 11 interactions.
    MINTi MINT-4496565.

    Chemistry

    BindingDBi P50107.
    ChEMBLi CHEMBL6057.

    Proteomic databases

    MaxQBi P50107.
    PaxDbi P50107.
    PeptideAtlasi P50107.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML004C ; YML004C ; YML004C .
    GeneIDi 855009.
    KEGGi sce:YML004C.

    Organism-specific databases

    CYGDi YML004c.
    SGDi S000004463. GLO1.

    Phylogenomic databases

    eggNOGi COG0346.
    GeneTreei ENSGT00390000009312.
    HOGENOMi HOG000215632.
    KOi K01759.
    OMAi TEGRMKN.
    OrthoDBi EOG7HXD33.

    Enzyme and pathway databases

    UniPathwayi UPA00619 ; UER00675 .
    BioCyci MetaCyc:MONOMER-624.
    YEAST:YML004C-MONOMER.
    SABIO-RK P50107.

    Miscellaneous databases

    NextBioi 978174.
    PROi P50107.

    Gene expression databases

    Genevestigatori P50107.

    Family and domain databases

    Gene3Di 3.10.180.10. 2 hits.
    InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR004360. Glyas_Fos-R_dOase_dom.
    IPR025870. Glyoxalase-like_dom.
    IPR004361. Glyoxalase_1.
    IPR018146. Glyoxalase_1_CS.
    [Graphical view ]
    Pfami PF00903. Glyoxalase. 1 hit.
    PF12681. Glyoxalase_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54593. SSF54593. 2 hits.
    TIGRFAMsi TIGR00068. glyox_I. 2 hits.
    PROSITEi PS00934. GLYOXALASE_I_1. 2 hits.
    PS00935. GLYOXALASE_I_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the structural gene for glyoxalase I from Saccharomyces cerevisiae."
      Inoue Y., Kimura A.
      J. Biol. Chem. 271:25958-25965(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. "Yeast glyoxalase I is a monomeric enzyme with two active sites."
      Frickel E.M., Jemth P., Widersten M., Mannervik B.
      J. Biol. Chem. 276:1845-1849(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Positive effect of GAC gene product on the mRNA level of glyoxalase I gene in Saccharomyces cerevisiae."
      Inoue Y., Yano H., Ginya H., Tsuchiyama H., Murata K., Kimura A.
      Biotechnol. Appl. Biochem. 14:391-394(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 310-326, PROTEIN SEQUENCE OF 39-45; 150-165 AND 311-323.
      Strain: ATCC 26109 / X2180.
    6. "The primary structure of monomeric yeast glyoxalase I indicates a gene duplication resulting in two similar segments homologous with the subunit of dimeric human glyoxalase I."
      Ridderstroem M., Mannervik B.
      Biochem. J. 316:1005-1006(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLGUL_YEAST
    AccessioniPrimary (citable) accession number: P50107
    Secondary accession number(s): D6VZH1, Q9HFG0, Q9URB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2570 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3