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P50105

- TAF4_YEAST

UniProt

P50105 - TAF4_YEAST

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Protein

Transcription initiation factor TFIID subunit 4

Gene

TAF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.7 Publications

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. DNA binding Source: UniProtKB
  3. RNA polymerase II activating transcription factor binding Source: SGD

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  3. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32716-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4
Alternative name(s):
MPT-1
TAF suppressor gene 2 protein
TAFII-48
TBP-associated factor 4
TBP-associated factor 48 kDa
Gene namesi
Name:TAF4
Synonyms:MPT1, TAF48, TSG2
Ordered Locus Names:YMR005W
ORF Names:YM8270.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR005w.
SGDiS000004607. TAF4.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Transcription initiation factor TFIID subunit 4PRO_0000118871Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Phosphoserine3 Publications
Modified residuei49 – 491Phosphoserine1 Publication
Modified residuei80 – 801Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50105.
PaxDbiP50105.
PeptideAtlasiP50105.

Expressioni

Gene expression databases

GenevestigatoriP50105.

Interactioni

Subunit structurei

TAF4 heterodimerizes with TAF12, forming ultimately an octamer consisting of a TAF6/TAF9 heterotetramer core flanked by TAF4/TAF12 dimers on either side, similar to the histone H2A/H2B/H3/H4 octamer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TAF1P466777EBI-11231,EBI-18855
TAF12Q037618EBI-11231,EBI-35097
TAF5P3812912EBI-11231,EBI-18868
TAF9Q0502710EBI-11231,EBI-27500

Protein-protein interaction databases

BioGridi35175. 81 interactions.
DIPiDIP-789N.
IntActiP50105. 33 interactions.
MINTiMINT-647326.
STRINGi4932.YMR005W.

Structurei

3D structure databases

ProteinModelPortaliP50105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini193 – 26169Histone-foldAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili279 – 29719Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TAF4 family.Curated
Contains 1 histone-fold domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG303189.
HOGENOMiHOG000154501.
InParanoidiP50105.
OMAiEEPGIVM.
OrthoDBiEOG7DFXNV.

Family and domain databases

InterProiIPR007900. TAF4.
[Graphical view]
PfamiPF05236. TAF4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50105-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANSPKKPSD GTGVSASDTP KYQHTVPETK PAFNLSPGKA SELSHSLPSP
60 70 80 90 100
SQIKSTAHVS STHNDAAGNT DDSVLPKNVS PTTNLRVESN GDTNNMFSSP
110 120 130 140 150
AGLALPKKDD KKKNKGTSKA DSKDGKASNS SGQNAQQQSD PNKMQDVLFS
160 170 180 190 200
AGIDVREEEA LLNSSINASK SQVQTNNVKI PNHLPFLHPE QVSNYMRKVG
210 220 230 240 250
KEQNFNLTPT KNPEILDMMS SACENYMRDI LTNAIVISRH RRKAVKINSG
260 270 280 290 300
RRSEVSAALR AIALIQKKEE ERRVKKRIAL GLEKEDYENK IDSEETLHRA
310 320 330 340 350
SNVTAGLRAG SKKQYGWLTS SVNKPTSLGA KSSGKVASDI TARGESGLKF
360 370 380
REAREEPGIV MRDLLFALEN RRNSVQTIIS KGYAKIRD
Length:388
Mass (Da):42,336
Last modified:October 1, 1996 - v1
Checksum:i7DFAC01C7AF07189
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79240 Genomic DNA. Translation: CAA55822.1.
Z48613 Genomic DNA. Translation: CAA88520.1.
BK006946 Genomic DNA. Translation: DAA09904.1.
PIRiS45013.
RefSeqiNP_013718.1. NM_001182501.1.

Genome annotation databases

EnsemblFungiiYMR005W; YMR005W; YMR005W.
GeneIDi855017.
KEGGisce:YMR005W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79240 Genomic DNA. Translation: CAA55822.1 .
Z48613 Genomic DNA. Translation: CAA88520.1 .
BK006946 Genomic DNA. Translation: DAA09904.1 .
PIRi S45013.
RefSeqi NP_013718.1. NM_001182501.1.

3D structure databases

ProteinModelPortali P50105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35175. 81 interactions.
DIPi DIP-789N.
IntActi P50105. 33 interactions.
MINTi MINT-647326.
STRINGi 4932.YMR005W.

Proteomic databases

MaxQBi P50105.
PaxDbi P50105.
PeptideAtlasi P50105.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR005W ; YMR005W ; YMR005W .
GeneIDi 855017.
KEGGi sce:YMR005W.

Organism-specific databases

CYGDi YMR005w.
SGDi S000004607. TAF4.

Phylogenomic databases

eggNOGi NOG303189.
HOGENOMi HOG000154501.
InParanoidi P50105.
OMAi EEPGIVM.
OrthoDBi EOG7DFXNV.

Enzyme and pathway databases

BioCyci YEAST:G3O-32716-MONOMER.

Miscellaneous databases

NextBioi 978198.

Gene expression databases

Genevestigatori P50105.

Family and domain databases

InterProi IPR007900. TAF4.
[Graphical view ]
Pfami PF05236. TAF4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Estey L.A., Douglas M.G.
    Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBY939.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
    Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
    Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
  5. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
    Sanders S.L., Weil P.A.
    J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  6. "Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
    Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
    Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
  7. "The histone fold is a key structural motif of transcription factor TFIID."
    Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
    Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
  8. "A histone fold TAF octamer within the yeast TFIID transcriptional coactivator."
    Selleck W., Howley R., Fang Q., Podolny V., Fried M.G., Buratowski S., Tan S.
    Nat. Struct. Biol. 8:695-700(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  9. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  10. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36; SER-49 AND SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAF4_YEAST
AccessioniPrimary (citable) accession number: P50105
Secondary accession number(s): D6VZI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3