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P50105

- TAF4_YEAST

UniProt

P50105 - TAF4_YEAST

Protein

Transcription initiation factor TFIID subunit 4

Gene

TAF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.7 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. DNA binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. RNA polymerase II activating transcription factor binding Source: SGD

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    3. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32716-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor TFIID subunit 4
    Alternative name(s):
    MPT-1
    TAF suppressor gene 2 protein
    TAFII-48
    TBP-associated factor 4
    TBP-associated factor 48 kDa
    Gene namesi
    Name:TAF4
    Synonyms:MPT1, TAF48, TSG2
    Ordered Locus Names:YMR005W
    ORF Names:YM8270.07
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR005w.
    SGDiS000004607. TAF4.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. transcription factor TFIID complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Transcription initiation factor TFIID subunit 4PRO_0000118871Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphoserine3 Publications
    Modified residuei49 – 491Phosphoserine1 Publication
    Modified residuei80 – 801Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP50105.
    PaxDbiP50105.
    PeptideAtlasiP50105.

    Expressioni

    Gene expression databases

    GenevestigatoriP50105.

    Interactioni

    Subunit structurei

    TAF4 heterodimerizes with TAF12, forming ultimately an octamer consisting of a TAF6/TAF9 heterotetramer core flanked by TAF4/TAF12 dimers on either side, similar to the histone H2A/H2B/H3/H4 octamer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TAF1P466777EBI-11231,EBI-18855
    TAF12Q037618EBI-11231,EBI-35097
    TAF5P3812912EBI-11231,EBI-18868
    TAF9Q0502710EBI-11231,EBI-27500

    Protein-protein interaction databases

    BioGridi35175. 81 interactions.
    DIPiDIP-789N.
    IntActiP50105. 33 interactions.
    MINTiMINT-647326.
    STRINGi4932.YMR005W.

    Structurei

    3D structure databases

    ProteinModelPortaliP50105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini193 – 26169Histone-foldAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili279 – 29719Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TAF4 family.Curated
    Contains 1 histone-fold domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG303189.
    HOGENOMiHOG000154501.
    OMAiEEPGIVM.
    OrthoDBiEOG7DFXNV.

    Family and domain databases

    InterProiIPR007900. TAF4.
    [Graphical view]
    PfamiPF05236. TAF4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50105-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANSPKKPSD GTGVSASDTP KYQHTVPETK PAFNLSPGKA SELSHSLPSP    50
    SQIKSTAHVS STHNDAAGNT DDSVLPKNVS PTTNLRVESN GDTNNMFSSP 100
    AGLALPKKDD KKKNKGTSKA DSKDGKASNS SGQNAQQQSD PNKMQDVLFS 150
    AGIDVREEEA LLNSSINASK SQVQTNNVKI PNHLPFLHPE QVSNYMRKVG 200
    KEQNFNLTPT KNPEILDMMS SACENYMRDI LTNAIVISRH RRKAVKINSG 250
    RRSEVSAALR AIALIQKKEE ERRVKKRIAL GLEKEDYENK IDSEETLHRA 300
    SNVTAGLRAG SKKQYGWLTS SVNKPTSLGA KSSGKVASDI TARGESGLKF 350
    REAREEPGIV MRDLLFALEN RRNSVQTIIS KGYAKIRD 388
    Length:388
    Mass (Da):42,336
    Last modified:October 1, 1996 - v1
    Checksum:i7DFAC01C7AF07189
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79240 Genomic DNA. Translation: CAA55822.1.
    Z48613 Genomic DNA. Translation: CAA88520.1.
    BK006946 Genomic DNA. Translation: DAA09904.1.
    PIRiS45013.
    RefSeqiNP_013718.1. NM_001182501.1.

    Genome annotation databases

    EnsemblFungiiYMR005W; YMR005W; YMR005W.
    GeneIDi855017.
    KEGGisce:YMR005W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79240 Genomic DNA. Translation: CAA55822.1 .
    Z48613 Genomic DNA. Translation: CAA88520.1 .
    BK006946 Genomic DNA. Translation: DAA09904.1 .
    PIRi S45013.
    RefSeqi NP_013718.1. NM_001182501.1.

    3D structure databases

    ProteinModelPortali P50105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35175. 81 interactions.
    DIPi DIP-789N.
    IntActi P50105. 33 interactions.
    MINTi MINT-647326.
    STRINGi 4932.YMR005W.

    Proteomic databases

    MaxQBi P50105.
    PaxDbi P50105.
    PeptideAtlasi P50105.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR005W ; YMR005W ; YMR005W .
    GeneIDi 855017.
    KEGGi sce:YMR005W.

    Organism-specific databases

    CYGDi YMR005w.
    SGDi S000004607. TAF4.

    Phylogenomic databases

    eggNOGi NOG303189.
    HOGENOMi HOG000154501.
    OMAi EEPGIVM.
    OrthoDBi EOG7DFXNV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32716-MONOMER.

    Miscellaneous databases

    NextBioi 978198.

    Gene expression databases

    Genevestigatori P50105.

    Family and domain databases

    InterProi IPR007900. TAF4.
    [Graphical view ]
    Pfami PF05236. TAF4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Estey L.A., Douglas M.G.
      Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DBY939.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
      Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
      Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
    5. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
      Sanders S.L., Weil P.A.
      J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    6. "Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
      Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
      Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
    7. "The histone fold is a key structural motif of transcription factor TFIID."
      Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
      Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
    8. "A histone fold TAF octamer within the yeast TFIID transcriptional coactivator."
      Selleck W., Howley R., Fang Q., Podolny V., Fried M.G., Buratowski S., Tan S.
      Nat. Struct. Biol. 8:695-700(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    9. "Molecular characterization of Saccharomyces cerevisiae TFIID."
      Sanders S.L., Garbett K.A., Weil P.A.
      Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TFIID STOICHIOMETRY.
    10. "Multi-protein complexes in eukaryotic gene transcription."
      Martinez E.
      Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
    14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    15. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36; SER-49 AND SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTAF4_YEAST
    AccessioniPrimary (citable) accession number: P50105
    Secondary accession number(s): D6VZI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3