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Protein

Transcription initiation factor TFIID subunit 4

Gene

TAF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.7 Publications

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA binding Source: UniProtKB
  • RNA polymerase II activating transcription factor binding Source: SGD
  • transcription coactivator activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32716-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4
Alternative name(s):
MPT-1
TAF suppressor gene 2 protein
TAFII-48
TBP-associated factor 4
TBP-associated factor 48 kDa
Gene namesi
Name:TAF4
Synonyms:MPT1, TAF48, TSG2
Ordered Locus Names:YMR005W
ORF Names:YM8270.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR005W
SGDiS000004607 TAF4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188711 – 388Transcription initiation factor TFIID subunit 4Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1
Modified residuei49PhosphoserineCombined sources1
Modified residuei80PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50105
PaxDbiP50105
PRIDEiP50105

PTM databases

iPTMnetiP50105

Interactioni

Subunit structurei

TAF4 heterodimerizes with TAF12, forming ultimately an octamer consisting of a TAF6/TAF9 heterotetramer core flanked by TAF4/TAF12 dimers on either side, similar to the histone H2A/H2B/H3/H4 octamer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi35175, 240 interactors
DIPiDIP-789N
IntActiP50105, 34 interactors
MINTiP50105
STRINGi4932.YMR005W

Structurei

3D structure databases

ProteinModelPortaliP50105
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 261Histone-foldAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili279 – 297Sequence analysisAdd BLAST19

Sequence similaritiesi

Belongs to the TAF4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000154501
InParanoidiP50105
OMAiEEPGIVM
OrthoDBiEOG092C3477

Family and domain databases

CDDicd08045 TAF4, 1 hit
InterProiView protein in InterPro
IPR007900 TAF4
PfamiView protein in Pfam
PF05236 TAF4, 1 hit

Sequencei

Sequence statusi: Complete.

P50105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSPKKPSD GTGVSASDTP KYQHTVPETK PAFNLSPGKA SELSHSLPSP
60 70 80 90 100
SQIKSTAHVS STHNDAAGNT DDSVLPKNVS PTTNLRVESN GDTNNMFSSP
110 120 130 140 150
AGLALPKKDD KKKNKGTSKA DSKDGKASNS SGQNAQQQSD PNKMQDVLFS
160 170 180 190 200
AGIDVREEEA LLNSSINASK SQVQTNNVKI PNHLPFLHPE QVSNYMRKVG
210 220 230 240 250
KEQNFNLTPT KNPEILDMMS SACENYMRDI LTNAIVISRH RRKAVKINSG
260 270 280 290 300
RRSEVSAALR AIALIQKKEE ERRVKKRIAL GLEKEDYENK IDSEETLHRA
310 320 330 340 350
SNVTAGLRAG SKKQYGWLTS SVNKPTSLGA KSSGKVASDI TARGESGLKF
360 370 380
REAREEPGIV MRDLLFALEN RRNSVQTIIS KGYAKIRD
Length:388
Mass (Da):42,336
Last modified:October 1, 1996 - v1
Checksum:i7DFAC01C7AF07189
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79240 Genomic DNA Translation: CAA55822.1
Z48613 Genomic DNA Translation: CAA88520.1
BK006946 Genomic DNA Translation: DAA09904.1
PIRiS45013
RefSeqiNP_013718.1, NM_001182501.1

Genome annotation databases

EnsemblFungiiYMR005W; YMR005W; YMR005W
GeneIDi855017
KEGGisce:YMR005W

Similar proteinsi

Entry informationi

Entry nameiTAF4_YEAST
AccessioniPrimary (citable) accession number: P50105
Secondary accession number(s): D6VZI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health