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P50103

- UCHL1_MONDO

UniProt

P50103 - UCHL1_MONDO

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Protein
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Gene
UCHL1
Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1 – 11Susceptible to oxidation By similarity
Sitei6 – 61Susceptible to oxidation By similarity
Sitei12 – 121Susceptible to oxidation By similarity
Active sitei90 – 901Nucleophile By similarity
Active sitei161 – 1611Proton donor By similarity
Sitei176 – 1761Important for enzyme activity By similarity
Sitei179 – 1791Susceptible to oxidation By similarity
Sitei220 – 2201Susceptible to oxidation By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: Ensembl
  2. ligase activity Source: UniProtKB-KW
  3. omega peptidase activity Source: Ensembl
  4. ubiquitin thiolesterase activity Source: Ensembl
  5. ubiquitin-specific protease activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. axon target recognition Source: Ensembl
  3. axon transport of mitochondrion Source: Ensembl
  4. cell proliferation Source: Ensembl
  5. eating behavior Source: Ensembl
  6. muscle fiber development Source: Ensembl
  7. negative regulation of MAP kinase activity Source: Ensembl
  8. neuromuscular process Source: Ensembl
  9. protein deubiquitination Source: Ensembl
  10. response to ischemia Source: Ensembl
  11. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
ProteomesiUP000002280: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. neuronal cell body Source: Ensembl
  5. nucleus Source: Ensembl
  6. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Ubiquitin carboxyl-terminal hydrolase isozyme L1
PRO_0000211057Add
BLAST
Propeptidei221 – 2233Removed in mature form By similarity
PRO_0000421570

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteine By similarity

Post-translational modificationi

O-glycosylated By similarity.

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Prenylation

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitin By similarity
Regioni151 – 1566Interaction with ubiquitin By similarity
Regioni211 – 2166Interaction with ubiquitin By similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

GeneTreeiENSGT00510000046640.
KOiK05611.
OMAiKSHENGH.
OrthoDBiEOG7S7SFK.
TreeFamiTF316166.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50103-1 [UniParc]FASTAAdd to Basket

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MQLKPMEINP EMLNKVLTRL GVGGDWKFVD VLGLEEDVLG TVPAPACALL    50
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTVGNSC GTIGLIHAVA 100
NNQDKLNFDD GSVLKQFISE TAKLSPEDRA KCFEKNEAIQ AAHDAVAQEG 150
QCRVDDEVNF HFILFNNVDG HLYELDGRMP FPINHGSNSD ESVLKGAAEI 200
CRQFTEREEG EVRFSAVALC KCA 223
Length:223
Mass (Da):24,767
Last modified:March 6, 2013 - v2
Checksum:i5EC27BD80CADF896
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32208 Genomic DNA. Translation: AAA89059.1.
RefSeqiXP_001365417.1. XM_001365380.3.

Genome annotation databases

EnsembliENSMODT00000026186; ENSMODP00000025726; ENSMODG00000020568.
GeneIDi100015629.
KEGGimdo:100015629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32208 Genomic DNA. Translation: AAA89059.1 .
RefSeqi XP_001365417.1. XM_001365380.3.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMODT00000026186 ; ENSMODP00000025726 ; ENSMODG00000020568 .
GeneIDi 100015629.
KEGGi mdo:100015629.

Organism-specific databases

CTDi 7345.

Phylogenomic databases

GeneTreei ENSGT00510000046640.
KOi K05611.
OMAi KSHENGH.
OrthoDBi EOG7S7SFK.
TreeFami TF316166.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of evolutionary conserved regulatory sequences in the 5' untranscribed region of the neural-specific ubiquitin C-terminal hydrolase (PGP9.5) gene."
    Mann D.A., Trowern A.R., Lavender F.L., Whittaker P.A., Thompson R.J.
    J. Neurochem. 66:35-46(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.

Entry informationi

Entry nameiUCHL1_MONDO
AccessioniPrimary (citable) accession number: P50103
Secondary accession number(s): F7APK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 6, 2013
Last modified: July 9, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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