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P50103

- UCHL1_MONDO

UniProt

P50103 - UCHL1_MONDO

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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophilePROSITE-ProRule annotation
Active sitei161 – 1611Proton donorPROSITE-ProRule annotation
Sitei176 – 1761Important for enzyme activityBy similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: Ensembl
  2. ligase activity Source: UniProtKB-KW
  3. omega peptidase activity Source: Ensembl
  4. ubiquitin-specific protease activity Source: InterPro
  5. ubiquitin thiolesterase activity Source: Ensembl

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. axon target recognition Source: Ensembl
  3. axon transport of mitochondrion Source: Ensembl
  4. cell proliferation Source: Ensembl
  5. eating behavior Source: Ensembl
  6. muscle fiber development Source: Ensembl
  7. negative regulation of MAP kinase activity Source: Ensembl
  8. neuromuscular process Source: Ensembl
  9. protein deubiquitination Source: Ensembl
  10. response to ischemia Source: Ensembl
  11. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
ProteomesiUP000002280: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: Ensembl
  5. neuronal cell body Source: Ensembl
  6. nucleus Source: Ensembl
  7. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211057Add
BLAST
Propeptidei221 – 2233Removed in mature formBy similarityPRO_0000421570

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteineBy similarity

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Prenylation

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitinBy similarity
Regioni151 – 1566Interaction with ubiquitinBy similarity
Regioni211 – 2166Interaction with ubiquitinBy similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000046640.
InParanoidiP50103.
KOiK05611.
OMAiKSHENGH.
OrthoDBiEOG7S7SFK.
TreeFamiTF316166.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50103-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQLKPMEINP EMLNKVLTRL GVGGDWKFVD VLGLEEDVLG TVPAPACALL
60 70 80 90 100
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTVGNSC GTIGLIHAVA
110 120 130 140 150
NNQDKLNFDD GSVLKQFISE TAKLSPEDRA KCFEKNEAIQ AAHDAVAQEG
160 170 180 190 200
QCRVDDEVNF HFILFNNVDG HLYELDGRMP FPINHGSNSD ESVLKGAAEI
210 220
CRQFTEREEG EVRFSAVALC KCA
Length:223
Mass (Da):24,767
Last modified:March 6, 2013 - v2
Checksum:i5EC27BD80CADF896
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32208 Genomic DNA. Translation: AAA89059.1.
RefSeqiXP_001365417.1. XM_001365380.3.

Genome annotation databases

EnsembliENSMODT00000026186; ENSMODP00000025726; ENSMODG00000020568.
GeneIDi100015629.
KEGGimdo:100015629.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32208 Genomic DNA. Translation: AAA89059.1 .
RefSeqi XP_001365417.1. XM_001365380.3.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C12.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMODT00000026186 ; ENSMODP00000025726 ; ENSMODG00000020568 .
GeneIDi 100015629.
KEGGi mdo:100015629.

Organism-specific databases

CTDi 7345.

Phylogenomic databases

GeneTreei ENSGT00510000046640.
InParanoidi P50103.
KOi K05611.
OMAi KSHENGH.
OrthoDBi EOG7S7SFK.
TreeFami TF316166.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of evolutionary conserved regulatory sequences in the 5' untranscribed region of the neural-specific ubiquitin C-terminal hydrolase (PGP9.5) gene."
    Mann D.A., Trowern A.R., Lavender F.L., Whittaker P.A., Thompson R.J.
    J. Neurochem. 66:35-46(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.

Entry informationi

Entry nameiUCHL1_MONDO
AccessioniPrimary (citable) accession number: P50103
Secondary accession number(s): F7APK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 6, 2013
Last modified: October 29, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3