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P50103

- UCHL1_MONDO

UniProt

P50103 - UCHL1_MONDO

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (06 Mar 2013)
      Previous versions | rss
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    Functioni

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1 – 11Susceptible to oxidationBy similarity
    Sitei6 – 61Susceptible to oxidationBy similarity
    Sitei12 – 121Susceptible to oxidationBy similarity
    Active sitei90 – 901NucleophilePROSITE-ProRule annotation
    Active sitei161 – 1611Proton donorPROSITE-ProRule annotation
    Sitei176 – 1761Important for enzyme activityBy similarity
    Sitei179 – 1791Susceptible to oxidationBy similarity
    Sitei220 – 2201Susceptible to oxidationBy similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: Ensembl
    2. ligase activity Source: UniProtKB-KW
    3. omega peptidase activity Source: Ensembl
    4. ubiquitin-specific protease activity Source: InterPro
    5. ubiquitin thiolesterase activity Source: Ensembl

    GO - Biological processi

    1. adult walking behavior Source: Ensembl
    2. axon target recognition Source: Ensembl
    3. axon transport of mitochondrion Source: Ensembl
    4. cell proliferation Source: Ensembl
    5. eating behavior Source: Ensembl
    6. muscle fiber development Source: Ensembl
    7. negative regulation of MAP kinase activity Source: Ensembl
    8. neuromuscular process Source: Ensembl
    9. protein deubiquitination Source: Ensembl
    10. response to ischemia Source: Ensembl
    11. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Ligase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
    Short name:
    UCH-L1
    Alternative name(s):
    Neuron cytoplasmic protein 9.5
    PGP 9.5
    Short name:
    PGP9.5
    Ubiquitin thioesterase L1
    Gene namesi
    Name:UCHL1
    OrganismiMonodelphis domestica (Gray short-tailed opossum)
    Taxonomic identifieri13616 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
    ProteomesiUP000002280: Chromosome 5

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. cytosol Source: Ensembl
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. neuronal cell body Source: Ensembl
    5. nucleus Source: Ensembl
    6. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 223223Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211057Add
    BLAST
    Propeptidei221 – 2233Removed in mature formBy similarityPRO_0000421570

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi220 – 2201S-farnesyl cysteineBy similarity

    Post-translational modificationi

    O-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Oxidation, Prenylation

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 106Interaction with ubiquitinBy similarity
    Regioni151 – 1566Interaction with ubiquitinBy similarity
    Regioni211 – 2166Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00510000046640.
    KOiK05611.
    OMAiKSHENGH.
    OrthoDBiEOG7S7SFK.
    TreeFamiTF316166.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.
    PROSITEiPS00140. UCH_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50103-1 [UniParc]FASTAAdd to Basket

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    MQLKPMEINP EMLNKVLTRL GVGGDWKFVD VLGLEEDVLG TVPAPACALL    50
    LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTVGNSC GTIGLIHAVA 100
    NNQDKLNFDD GSVLKQFISE TAKLSPEDRA KCFEKNEAIQ AAHDAVAQEG 150
    QCRVDDEVNF HFILFNNVDG HLYELDGRMP FPINHGSNSD ESVLKGAAEI 200
    CRQFTEREEG EVRFSAVALC KCA 223
    Length:223
    Mass (Da):24,767
    Last modified:March 6, 2013 - v2
    Checksum:i5EC27BD80CADF896
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32208 Genomic DNA. Translation: AAA89059.1.
    RefSeqiXP_001365417.1. XM_001365380.3.

    Genome annotation databases

    EnsembliENSMODT00000026186; ENSMODP00000025726; ENSMODG00000020568.
    GeneIDi100015629.
    KEGGimdo:100015629.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32208 Genomic DNA. Translation: AAA89059.1 .
    RefSeqi XP_001365417.1. XM_001365380.3.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMODT00000026186 ; ENSMODP00000025726 ; ENSMODG00000020568 .
    GeneIDi 100015629.
    KEGGi mdo:100015629.

    Organism-specific databases

    CTDi 7345.

    Phylogenomic databases

    GeneTreei ENSGT00510000046640.
    KOi K05611.
    OMAi KSHENGH.
    OrthoDBi EOG7S7SFK.
    TreeFami TF316166.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    PROSITEi PS00140. UCH_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of evolutionary conserved regulatory sequences in the 5' untranscribed region of the neural-specific ubiquitin C-terminal hydrolase (PGP9.5) gene."
      Mann D.A., Trowern A.R., Lavender F.L., Whittaker P.A., Thompson R.J.
      J. Neurochem. 66:35-46(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiUCHL1_MONDO
    AccessioniPrimary (citable) accession number: P50103
    Secondary accession number(s): F7APK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: March 6, 2013
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3