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Protein

Ubiquitin carboxyl-terminal hydrolase 15

Gene

UBP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei214 – 2141NucleophilePROSITE-ProRule annotation
Active sitei465 – 4651Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  1. protein deubiquitination Source: SGD
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32969-MONOMER.

Protein family/group databases

MEROPSiC19.099.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 15 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 15
Ubiquitin thioesterase 15
Ubiquitin-specific-processing protease 15
Gene namesi
Name:UBP15
Ordered Locus Names:YMR304W
ORF Names:YM9952.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR304w.
SGDiS000004920. UBP15.

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12301230Ubiquitin carboxyl-terminal hydrolase 15PRO_0000080600Add
BLAST

Proteomic databases

MaxQBiP50101.
PaxDbiP50101.
PeptideAtlasiP50101.

Expressioni

Gene expression databases

GenevestigatoriP50101.

Interactioni

Protein-protein interaction databases

BioGridi35484. 122 interactions.
DIPiDIP-6313N.
IntActiP50101. 33 interactions.
MINTiMINT-618224.
STRINGi4932.YMR304W.

Structurei

3D structure databases

ProteinModelPortaliP50101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 179141MATHPROSITE-ProRule annotationAdd
BLAST
Domaini205 – 536332USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 MATH domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119158.
HOGENOMiHOG000160240.
InParanoidiP50101.
KOiK11838.
OMAiVENDCAR.
OrthoDBiEOG7C2R8P.

Family and domain databases

InterProiIPR002083. MATH.
IPR001394. Peptidase_C19_UCH.
IPR008974. TRAF-like.
IPR024729. USP7_ICP0-binding_dom.
IPR029346. USP_C.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00917. MATH. 1 hit.
PF00443. UCH. 1 hit.
PF14533. USP7_C2. 1 hit.
PF12436. USP7_ICP0_bdg. 1 hit.
[Graphical view]
SMARTiSM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
PROSITEiPS50144. MATH. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEDELGSI GTVFPGSPID KSIGSILPQF DEEVETLLED SFTWNIPDWN
60 70 80 90 100
ELTNPKYNSP RFRIGDFEWD ILLFPQGNHN KGVAVYLEPH PEEKLDETTG
110 120 130 140 150
EMVPVDPDWY CCAQFAIGIS RPGNGDTINL INKSHHRFNA LDTDWGFANL
160 170 180 190 200
IDLNNLKHPS KGRPLSFLNE GTLNITAYVR ILKDPTGVLW HNFLNYDSKK
210 220 230 240 250
VTGYVGFRNQ GATCYLNSLL QSYFFTKYFR KLVYEIPTEH ESPNNSVPLA
260 270 280 290 300
LQRAFYQLQV SDIPLDTLEL TRSFGWDTAE SFTQHDVQEL NRILMDRLEN
310 320 330 340 350
NMKGTPVEGK LNEIFVGKMK SYIKCINVDY ESARVEDFWD LQLNVKNFKN
360 370 380 390 400
LQESFDNYIE MELMNGENQY AAQDYGLQDA QKGVIFESFP PVLHLQLKRF
410 420 430 440 450
EYDFNYDQMV KVNDKYEFPE TIDLSPFVDK DVLKKTLDSE NKDKNPYVYN
460 470 480 490 500
LHGVLVHSGD ISTGHYYTLI KPGVEDQWYR FDDERVWRVT KKQVFQENFG
510 520 530 540 550
CDRLPDEKVR TMTRGEYQNY IIQRHTSAYM LVYIRQEQEE DLLRPVLESD
560 570 580 590 600
VPKHVITRVR EEIKERETKE KEIREAHLYV TLRLHSIKEF IHYEGFDYFA
610 620 630 640 650
HDGFRLFAEE LNDSGLQQIN LKVLRTTKLS DIFASIKETM NIPQERDVKY
660 670 680 690 700
WKMDYRRNST LRLTQPINFE SVNITLQEAL KKEKKRTMQT QYGEEGVAST
710 720 730 740 750
EEDDKALLET VSFLDLFIEE PYLELQFLNK LKEASLISKA QLDDELISTI
760 770 780 790 800
RTNLPELTKG GIEPVFATDN KSNLLFVKSY DPHTQKLLGF GHFAVNQLQQ
810 820 830 840 850
LSDISAIIED SISSNEKLTF YEEVQPGTIN EIYMKETIYD ADIDTGDIVS
860 870 880 890 900
FEVPGAVLPD TFPVYATIKD FYSYLRYRVK LKFSKFDGSS EEYGVSNEIP
910 920 930 940 950
ESFEFWISAY APYDDLARMV SKYAHVKPEY LKIIALYSNG RFVLKSTSLL
960 970 980 990 1000
NDYLLKDFNC DQIPPFAFEV LSVPLKELER LRPIKLYWLK NSYIHYQCFE
1010 1020 1030 1040 1050
FEVANDYTES QFLEKVQHKI GFTDEEKENI LLWTNTNFQF QGLLSDQNTF
1060 1070 1080 1090 1100
KDVSKHSLLF GRILPEESKL FKELNRLENV QTSSLEDFMD DENATDRPMD
1110 1120 1130 1140 1150
DEQDLGMAIE HSEDMKGRIV VVQQYFKDLE NRHGISFLFN LIPDETFPKT
1160 1170 1180 1190 1200
KDRLHAKFGL GQKEFSKIKL SIGYSTEEGT VFRSLQGFSD EELDKVILYD
1210 1220 1230
IMSNLDYIYM DHPDRLRSHS SYDRPMIIKN
Length:1,230
Mass (Da):143,564
Last modified:September 30, 1996 - v1
Checksum:iBEB8F6E3DD71A0CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA. Translation: CAA89137.1.
BK006946 Genomic DNA. Translation: DAA10205.1.
PIRiS53974.
RefSeqiNP_014033.1. NM_001182814.1.

Genome annotation databases

EnsemblFungiiYMR304W; YMR304W; YMR304W.
GeneIDi855350.
KEGGisce:YMR304W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA. Translation: CAA89137.1.
BK006946 Genomic DNA. Translation: DAA10205.1.
PIRiS53974.
RefSeqiNP_014033.1. NM_001182814.1.

3D structure databases

ProteinModelPortaliP50101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35484. 122 interactions.
DIPiDIP-6313N.
IntActiP50101. 33 interactions.
MINTiMINT-618224.
STRINGi4932.YMR304W.

Protein family/group databases

MEROPSiC19.099.

Proteomic databases

MaxQBiP50101.
PaxDbiP50101.
PeptideAtlasiP50101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR304W; YMR304W; YMR304W.
GeneIDi855350.
KEGGisce:YMR304W.

Organism-specific databases

CYGDiYMR304w.
SGDiS000004920. UBP15.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00760000119158.
HOGENOMiHOG000160240.
InParanoidiP50101.
KOiK11838.
OMAiVENDCAR.
OrthoDBiEOG7C2R8P.

Enzyme and pathway databases

BioCyciYEAST:G3O-32969-MONOMER.

Miscellaneous databases

NextBioi979106.
PROiP50101.

Gene expression databases

GenevestigatoriP50101.

Family and domain databases

InterProiIPR002083. MATH.
IPR001394. Peptidase_C19_UCH.
IPR008974. TRAF-like.
IPR024729. USP7_ICP0-binding_dom.
IPR029346. USP_C.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00917. MATH. 1 hit.
PF00443. UCH. 1 hit.
PF14533. USP7_C2. 1 hit.
PF12436. USP7_ICP0_bdg. 1 hit.
[Graphical view]
SMARTiSM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
PROSITEiPS50144. MATH. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP15_YEAST
AccessioniPrimary (citable) accession number: P50101
Secondary accession number(s): D6W0D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: March 3, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.