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Protein

Ubiquitin carboxyl-terminal hydrolase 15

Gene

UBP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 2810 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei214NucleophilePROSITE-ProRule annotation1
Active sitei465Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • free ubiquitin chain depolymerization Source: SGD
  • protein deubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32969-MONOMER
ReactomeiR-SCE-1169408 ISG15 antiviral mechanism
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes

Protein family/group databases

MEROPSiC19.099

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 15 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 15
Ubiquitin thioesterase 15
Ubiquitin-specific-processing protease 15
Gene namesi
Name:UBP15
Ordered Locus Names:YMR304W
ORF Names:YM9952.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR304W
SGDiS000004920 UBP15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806001 – 1230Ubiquitin carboxyl-terminal hydrolase 15Add BLAST1230

Proteomic databases

MaxQBiP50101
PaxDbiP50101
PRIDEiP50101

PTM databases

iPTMnetiP50101

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35484, 180 interactors
DIPiDIP-6313N
IntActiP50101, 51 interactors
MINTiP50101
STRINGi4932.YMR304W

Structurei

3D structure databases

ProteinModelPortaliP50101
SMRiP50101
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 179MATHPROSITE-ProRule annotationAdd BLAST141
Domaini205 – 536USPAdd BLAST332

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119158
HOGENOMiHOG000160240
InParanoidiP50101
KOiK11838
OMAiPDKEWEN
OrthoDBiEOG092C06CQ

Family and domain databases

Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR002083 MATH/TRAF_dom
IPR001394 Peptidase_C19_UCH
IPR008974 TRAF-like
IPR024729 USP7_ICP0-binding_dom
IPR029346 USP_C
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PF14533 USP7_C2, 1 hit
PF12436 USP7_ICP0_bdg, 1 hit
SMARTiView protein in SMART
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50144 MATH, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

P50101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEDELGSI GTVFPGSPID KSIGSILPQF DEEVETLLED SFTWNIPDWN
60 70 80 90 100
ELTNPKYNSP RFRIGDFEWD ILLFPQGNHN KGVAVYLEPH PEEKLDETTG
110 120 130 140 150
EMVPVDPDWY CCAQFAIGIS RPGNGDTINL INKSHHRFNA LDTDWGFANL
160 170 180 190 200
IDLNNLKHPS KGRPLSFLNE GTLNITAYVR ILKDPTGVLW HNFLNYDSKK
210 220 230 240 250
VTGYVGFRNQ GATCYLNSLL QSYFFTKYFR KLVYEIPTEH ESPNNSVPLA
260 270 280 290 300
LQRAFYQLQV SDIPLDTLEL TRSFGWDTAE SFTQHDVQEL NRILMDRLEN
310 320 330 340 350
NMKGTPVEGK LNEIFVGKMK SYIKCINVDY ESARVEDFWD LQLNVKNFKN
360 370 380 390 400
LQESFDNYIE MELMNGENQY AAQDYGLQDA QKGVIFESFP PVLHLQLKRF
410 420 430 440 450
EYDFNYDQMV KVNDKYEFPE TIDLSPFVDK DVLKKTLDSE NKDKNPYVYN
460 470 480 490 500
LHGVLVHSGD ISTGHYYTLI KPGVEDQWYR FDDERVWRVT KKQVFQENFG
510 520 530 540 550
CDRLPDEKVR TMTRGEYQNY IIQRHTSAYM LVYIRQEQEE DLLRPVLESD
560 570 580 590 600
VPKHVITRVR EEIKERETKE KEIREAHLYV TLRLHSIKEF IHYEGFDYFA
610 620 630 640 650
HDGFRLFAEE LNDSGLQQIN LKVLRTTKLS DIFASIKETM NIPQERDVKY
660 670 680 690 700
WKMDYRRNST LRLTQPINFE SVNITLQEAL KKEKKRTMQT QYGEEGVAST
710 720 730 740 750
EEDDKALLET VSFLDLFIEE PYLELQFLNK LKEASLISKA QLDDELISTI
760 770 780 790 800
RTNLPELTKG GIEPVFATDN KSNLLFVKSY DPHTQKLLGF GHFAVNQLQQ
810 820 830 840 850
LSDISAIIED SISSNEKLTF YEEVQPGTIN EIYMKETIYD ADIDTGDIVS
860 870 880 890 900
FEVPGAVLPD TFPVYATIKD FYSYLRYRVK LKFSKFDGSS EEYGVSNEIP
910 920 930 940 950
ESFEFWISAY APYDDLARMV SKYAHVKPEY LKIIALYSNG RFVLKSTSLL
960 970 980 990 1000
NDYLLKDFNC DQIPPFAFEV LSVPLKELER LRPIKLYWLK NSYIHYQCFE
1010 1020 1030 1040 1050
FEVANDYTES QFLEKVQHKI GFTDEEKENI LLWTNTNFQF QGLLSDQNTF
1060 1070 1080 1090 1100
KDVSKHSLLF GRILPEESKL FKELNRLENV QTSSLEDFMD DENATDRPMD
1110 1120 1130 1140 1150
DEQDLGMAIE HSEDMKGRIV VVQQYFKDLE NRHGISFLFN LIPDETFPKT
1160 1170 1180 1190 1200
KDRLHAKFGL GQKEFSKIKL SIGYSTEEGT VFRSLQGFSD EELDKVILYD
1210 1220 1230
IMSNLDYIYM DHPDRLRSHS SYDRPMIIKN
Length:1,230
Mass (Da):143,564
Last modified:October 1, 1996 - v1
Checksum:iBEB8F6E3DD71A0CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49212 Genomic DNA Translation: CAA89137.1
BK006946 Genomic DNA Translation: DAA10205.1
PIRiS53974
RefSeqiNP_014033.1, NM_001182814.1

Genome annotation databases

EnsemblFungiiYMR304W; YMR304W; YMR304W
GeneIDi855350
KEGGisce:YMR304W

Similar proteinsi

Entry informationi

Entry nameiUBP15_YEAST
AccessioniPrimary (citable) accession number: P50101
Secondary accession number(s): D6W0D1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health