P50101 (UBP15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 15
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 15
Ubiquitin thioesterase 15
Ubiquitin-specific-processing protease 15

Gene names

Name:	UBP15
Ordered Locus Names:	YMR304W
ORF Names:	YM9952.06

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1230 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Miscellaneous	Present with 2810 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 MATH domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from mutant phenotype PubMed 21665945. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	peroxisome Inferred from direct assay PubMed 21665945. Source: SGD
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay PubMed 10527495PubMed 21710968. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1230	1230	Ubiquitin carboxyl-terminal hydrolase 15		PRO_0000080600
Regions
	Domain	39 – 179	141	MATH
Sites
	Active site	214	1	Nucleophile By similarity
	Active site	465	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50101 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BEB8F6E3DD71A0CC	FASTA	1,230	143,564
	10 20 30 40 50 60 MSSEDELGSI GTVFPGSPID KSIGSILPQF DEEVETLLED SFTWNIPDWN ELTNPKYNSP 70 80 90 100 110 120 RFRIGDFEWD ILLFPQGNHN KGVAVYLEPH PEEKLDETTG EMVPVDPDWY CCAQFAIGIS 130 140 150 160 170 180 RPGNGDTINL INKSHHRFNA LDTDWGFANL IDLNNLKHPS KGRPLSFLNE GTLNITAYVR 190 200 210 220 230 240 ILKDPTGVLW HNFLNYDSKK VTGYVGFRNQ GATCYLNSLL QSYFFTKYFR KLVYEIPTEH 250 260 270 280 290 300 ESPNNSVPLA LQRAFYQLQV SDIPLDTLEL TRSFGWDTAE SFTQHDVQEL NRILMDRLEN 310 320 330 340 350 360 NMKGTPVEGK LNEIFVGKMK SYIKCINVDY ESARVEDFWD LQLNVKNFKN LQESFDNYIE 370 380 390 400 410 420 MELMNGENQY AAQDYGLQDA QKGVIFESFP PVLHLQLKRF EYDFNYDQMV KVNDKYEFPE 430 440 450 460 470 480 TIDLSPFVDK DVLKKTLDSE NKDKNPYVYN LHGVLVHSGD ISTGHYYTLI KPGVEDQWYR 490 500 510 520 530 540 FDDERVWRVT KKQVFQENFG CDRLPDEKVR TMTRGEYQNY IIQRHTSAYM LVYIRQEQEE 550 560 570 580 590 600 DLLRPVLESD VPKHVITRVR EEIKERETKE KEIREAHLYV TLRLHSIKEF IHYEGFDYFA 610 620 630 640 650 660 HDGFRLFAEE LNDSGLQQIN LKVLRTTKLS DIFASIKETM NIPQERDVKY WKMDYRRNST 670 680 690 700 710 720 LRLTQPINFE SVNITLQEAL KKEKKRTMQT QYGEEGVAST EEDDKALLET VSFLDLFIEE 730 740 750 760 770 780 PYLELQFLNK LKEASLISKA QLDDELISTI RTNLPELTKG GIEPVFATDN KSNLLFVKSY 790 800 810 820 830 840 DPHTQKLLGF GHFAVNQLQQ LSDISAIIED SISSNEKLTF YEEVQPGTIN EIYMKETIYD 850 860 870 880 890 900 ADIDTGDIVS FEVPGAVLPD TFPVYATIKD FYSYLRYRVK LKFSKFDGSS EEYGVSNEIP 910 920 930 940 950 960 ESFEFWISAY APYDDLARMV SKYAHVKPEY LKIIALYSNG RFVLKSTSLL NDYLLKDFNC 970 980 990 1000 1010 1020 DQIPPFAFEV LSVPLKELER LRPIKLYWLK NSYIHYQCFE FEVANDYTES QFLEKVQHKI 1030 1040 1050 1060 1070 1080 GFTDEEKENI LLWTNTNFQF QGLLSDQNTF KDVSKHSLLF GRILPEESKL FKELNRLENV 1090 1100 1110 1120 1130 1140 QTSSLEDFMD DENATDRPMD DEQDLGMAIE HSEDMKGRIV VVQQYFKDLE NRHGISFLFN 1150 1160 1170 1180 1190 1200 LIPDETFPKT KDRLHAKFGL GQKEFSKIKL SIGYSTEEGT VFRSLQGFSD EELDKVILYD 1210 1220 1230 IMSNLDYIYM DHPDRLRSHS SYDRPMIIKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49212 Genomic DNA. Translation: CAA89137.1. BK006946 Genomic DNA. Translation: DAA10205.1.
PIR	S53974.
RefSeq	NP_014033.1. NM_001182814.1.
3D structure databases
ProteinModelPortal	P50101.
SMR	P50101. Positions 39-1172.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6313N.
IntAct	P50101. 39 interactions.
MINT	MINT-618224.
STRING	4932.YMR304W.
Protein family/group databases
MEROPS	C19.099.
Proteomic databases
PaxDb	P50101.
PeptideAtlas	P50101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR304W; YMR304W; YMR304W.
GeneID	855350.
KEGG	sce:YMR304W.
Organism-specific databases
CYGD	YMR304w.
SGD	S000004920. UBP15.
Phylogenomic databases
eggNOG	COG5077.
GeneTree	ENSGT00630000089581.
HOGENOM	HOG000160240.
KO	K11838.
OMA	GHYYAMI.
OrthoDB	EOG4ZW8K5.
Gene expression databases
Genevestigator	P50101.
GermOnline	YMR304W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002083. MATH. IPR024729. Pept_C19_dom. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR008974. TRAF-like. [Graphical view]
Pfam	PF00917. MATH. 1 hit. PF00443. UCH. 1 hit. PF12436. USP7. 1 hit. [Graphical view]
SMART	SM00061. MATH. 1 hit. [Graphical view]
SUPFAM	SSF49599. Traf_like. 1 hit.
PROSITE	PS50144. MATH. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979106.

Entry information

Entry name

UBP15_YEAST

Accession

Primary (citable) accession number: P50101
Secondary accession number(s): D6W0D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program