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P50101

- UBP15_YEAST

UniProt

P50101 - UBP15_YEAST

Protein

Ubiquitin carboxyl-terminal hydrolase 15

Gene

UBP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei214 – 2141NucleophilePROSITE-ProRule annotation
    Active sitei465 – 4651Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: SGD

    GO - Biological processi

    1. protein deubiquitination Source: SGD
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32969-MONOMER.

    Protein family/group databases

    MEROPSiC19.099.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 15 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 15
    Ubiquitin thioesterase 15
    Ubiquitin-specific-processing protease 15
    Gene namesi
    Name:UBP15
    Ordered Locus Names:YMR304W
    ORF Names:YM9952.06
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR304w.
    SGDiS000004920. UBP15.

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisome Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12301230Ubiquitin carboxyl-terminal hydrolase 15PRO_0000080600Add
    BLAST

    Proteomic databases

    MaxQBiP50101.
    PaxDbiP50101.
    PeptideAtlasiP50101.

    Expressioni

    Gene expression databases

    GenevestigatoriP50101.

    Interactioni

    Protein-protein interaction databases

    BioGridi35484. 118 interactions.
    DIPiDIP-6313N.
    IntActiP50101. 32 interactions.
    MINTiMINT-618224.
    STRINGi4932.YMR304W.

    Structurei

    3D structure databases

    ProteinModelPortaliP50101.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 179141MATHPROSITE-ProRule annotationAdd
    BLAST
    Domaini205 – 536332USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 MATH domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    GeneTreeiENSGT00740000115066.
    HOGENOMiHOG000160240.
    KOiK11838.
    OMAiFDDERVW.
    OrthoDBiEOG7C2R8P.

    Family and domain databases

    InterProiIPR002083. MATH.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR008974. TRAF-like.
    IPR028889. UCH/PAN2.
    IPR024729. USP7_ICP0-binding_dom.
    IPR029346. USP_C.
    [Graphical view]
    PfamiPF00917. MATH. 1 hit.
    PF00443. UCH. 1 hit.
    PF14533. USP7_C2. 1 hit.
    PF12436. USP7_ICP0_bdg. 1 hit.
    [Graphical view]
    SMARTiSM00061. MATH. 1 hit.
    [Graphical view]
    SUPFAMiSSF49599. SSF49599. 1 hit.
    PROSITEiPS50144. MATH. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50101-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSEDELGSI GTVFPGSPID KSIGSILPQF DEEVETLLED SFTWNIPDWN     50
    ELTNPKYNSP RFRIGDFEWD ILLFPQGNHN KGVAVYLEPH PEEKLDETTG 100
    EMVPVDPDWY CCAQFAIGIS RPGNGDTINL INKSHHRFNA LDTDWGFANL 150
    IDLNNLKHPS KGRPLSFLNE GTLNITAYVR ILKDPTGVLW HNFLNYDSKK 200
    VTGYVGFRNQ GATCYLNSLL QSYFFTKYFR KLVYEIPTEH ESPNNSVPLA 250
    LQRAFYQLQV SDIPLDTLEL TRSFGWDTAE SFTQHDVQEL NRILMDRLEN 300
    NMKGTPVEGK LNEIFVGKMK SYIKCINVDY ESARVEDFWD LQLNVKNFKN 350
    LQESFDNYIE MELMNGENQY AAQDYGLQDA QKGVIFESFP PVLHLQLKRF 400
    EYDFNYDQMV KVNDKYEFPE TIDLSPFVDK DVLKKTLDSE NKDKNPYVYN 450
    LHGVLVHSGD ISTGHYYTLI KPGVEDQWYR FDDERVWRVT KKQVFQENFG 500
    CDRLPDEKVR TMTRGEYQNY IIQRHTSAYM LVYIRQEQEE DLLRPVLESD 550
    VPKHVITRVR EEIKERETKE KEIREAHLYV TLRLHSIKEF IHYEGFDYFA 600
    HDGFRLFAEE LNDSGLQQIN LKVLRTTKLS DIFASIKETM NIPQERDVKY 650
    WKMDYRRNST LRLTQPINFE SVNITLQEAL KKEKKRTMQT QYGEEGVAST 700
    EEDDKALLET VSFLDLFIEE PYLELQFLNK LKEASLISKA QLDDELISTI 750
    RTNLPELTKG GIEPVFATDN KSNLLFVKSY DPHTQKLLGF GHFAVNQLQQ 800
    LSDISAIIED SISSNEKLTF YEEVQPGTIN EIYMKETIYD ADIDTGDIVS 850
    FEVPGAVLPD TFPVYATIKD FYSYLRYRVK LKFSKFDGSS EEYGVSNEIP 900
    ESFEFWISAY APYDDLARMV SKYAHVKPEY LKIIALYSNG RFVLKSTSLL 950
    NDYLLKDFNC DQIPPFAFEV LSVPLKELER LRPIKLYWLK NSYIHYQCFE 1000
    FEVANDYTES QFLEKVQHKI GFTDEEKENI LLWTNTNFQF QGLLSDQNTF 1050
    KDVSKHSLLF GRILPEESKL FKELNRLENV QTSSLEDFMD DENATDRPMD 1100
    DEQDLGMAIE HSEDMKGRIV VVQQYFKDLE NRHGISFLFN LIPDETFPKT 1150
    KDRLHAKFGL GQKEFSKIKL SIGYSTEEGT VFRSLQGFSD EELDKVILYD 1200
    IMSNLDYIYM DHPDRLRSHS SYDRPMIIKN 1230
    Length:1,230
    Mass (Da):143,564
    Last modified:October 1, 1996 - v1
    Checksum:iBEB8F6E3DD71A0CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49212 Genomic DNA. Translation: CAA89137.1.
    BK006946 Genomic DNA. Translation: DAA10205.1.
    PIRiS53974.
    RefSeqiNP_014033.1. NM_001182814.1.

    Genome annotation databases

    EnsemblFungiiYMR304W; YMR304W; YMR304W.
    GeneIDi855350.
    KEGGisce:YMR304W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49212 Genomic DNA. Translation: CAA89137.1 .
    BK006946 Genomic DNA. Translation: DAA10205.1 .
    PIRi S53974.
    RefSeqi NP_014033.1. NM_001182814.1.

    3D structure databases

    ProteinModelPortali P50101.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35484. 118 interactions.
    DIPi DIP-6313N.
    IntActi P50101. 32 interactions.
    MINTi MINT-618224.
    STRINGi 4932.YMR304W.

    Protein family/group databases

    MEROPSi C19.099.

    Proteomic databases

    MaxQBi P50101.
    PaxDbi P50101.
    PeptideAtlasi P50101.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR304W ; YMR304W ; YMR304W .
    GeneIDi 855350.
    KEGGi sce:YMR304W.

    Organism-specific databases

    CYGDi YMR304w.
    SGDi S000004920. UBP15.

    Phylogenomic databases

    eggNOGi COG5077.
    GeneTreei ENSGT00740000115066.
    HOGENOMi HOG000160240.
    KOi K11838.
    OMAi FDDERVW.
    OrthoDBi EOG7C2R8P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32969-MONOMER.

    Miscellaneous databases

    NextBioi 979106.
    PROi P50101.

    Gene expression databases

    Genevestigatori P50101.

    Family and domain databases

    InterProi IPR002083. MATH.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR008974. TRAF-like.
    IPR028889. UCH/PAN2.
    IPR024729. USP7_ICP0-binding_dom.
    IPR029346. USP_C.
    [Graphical view ]
    Pfami PF00917. MATH. 1 hit.
    PF00443. UCH. 1 hit.
    PF14533. USP7_C2. 1 hit.
    PF12436. USP7_ICP0_bdg. 1 hit.
    [Graphical view ]
    SMARTi SM00061. MATH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49599. SSF49599. 1 hit.
    PROSITEi PS50144. MATH. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    4. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBP15_YEAST
    AccessioniPrimary (citable) accession number: P50101
    Secondary accession number(s): D6W0D1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2810 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3