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P50101 (UBP15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 15

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 15
Ubiquitin thioesterase 15
Ubiquitin-specific-processing protease 15
Gene names
Name:UBP15
Ordered Locus Names:YMR304W
ORF Names:YM9952.06
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1230 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Miscellaneous

Present with 2810 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 MATH domain.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12301230Ubiquitin carboxyl-terminal hydrolase 15
PRO_0000080600

Regions

Domain39 – 179141MATH
Domain205 – 536332USP

Sites

Active site2141Nucleophile By similarity
Active site4651Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
P50101 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BEB8F6E3DD71A0CC

FASTA1,230143,564
        10         20         30         40         50         60 
MSSEDELGSI GTVFPGSPID KSIGSILPQF DEEVETLLED SFTWNIPDWN ELTNPKYNSP 

        70         80         90        100        110        120 
RFRIGDFEWD ILLFPQGNHN KGVAVYLEPH PEEKLDETTG EMVPVDPDWY CCAQFAIGIS 

       130        140        150        160        170        180 
RPGNGDTINL INKSHHRFNA LDTDWGFANL IDLNNLKHPS KGRPLSFLNE GTLNITAYVR 

       190        200        210        220        230        240 
ILKDPTGVLW HNFLNYDSKK VTGYVGFRNQ GATCYLNSLL QSYFFTKYFR KLVYEIPTEH 

       250        260        270        280        290        300 
ESPNNSVPLA LQRAFYQLQV SDIPLDTLEL TRSFGWDTAE SFTQHDVQEL NRILMDRLEN 

       310        320        330        340        350        360 
NMKGTPVEGK LNEIFVGKMK SYIKCINVDY ESARVEDFWD LQLNVKNFKN LQESFDNYIE 

       370        380        390        400        410        420 
MELMNGENQY AAQDYGLQDA QKGVIFESFP PVLHLQLKRF EYDFNYDQMV KVNDKYEFPE 

       430        440        450        460        470        480 
TIDLSPFVDK DVLKKTLDSE NKDKNPYVYN LHGVLVHSGD ISTGHYYTLI KPGVEDQWYR 

       490        500        510        520        530        540 
FDDERVWRVT KKQVFQENFG CDRLPDEKVR TMTRGEYQNY IIQRHTSAYM LVYIRQEQEE 

       550        560        570        580        590        600 
DLLRPVLESD VPKHVITRVR EEIKERETKE KEIREAHLYV TLRLHSIKEF IHYEGFDYFA 

       610        620        630        640        650        660 
HDGFRLFAEE LNDSGLQQIN LKVLRTTKLS DIFASIKETM NIPQERDVKY WKMDYRRNST 

       670        680        690        700        710        720 
LRLTQPINFE SVNITLQEAL KKEKKRTMQT QYGEEGVAST EEDDKALLET VSFLDLFIEE 

       730        740        750        760        770        780 
PYLELQFLNK LKEASLISKA QLDDELISTI RTNLPELTKG GIEPVFATDN KSNLLFVKSY 

       790        800        810        820        830        840 
DPHTQKLLGF GHFAVNQLQQ LSDISAIIED SISSNEKLTF YEEVQPGTIN EIYMKETIYD 

       850        860        870        880        890        900 
ADIDTGDIVS FEVPGAVLPD TFPVYATIKD FYSYLRYRVK LKFSKFDGSS EEYGVSNEIP 

       910        920        930        940        950        960 
ESFEFWISAY APYDDLARMV SKYAHVKPEY LKIIALYSNG RFVLKSTSLL NDYLLKDFNC 

       970        980        990       1000       1010       1020 
DQIPPFAFEV LSVPLKELER LRPIKLYWLK NSYIHYQCFE FEVANDYTES QFLEKVQHKI 

      1030       1040       1050       1060       1070       1080 
GFTDEEKENI LLWTNTNFQF QGLLSDQNTF KDVSKHSLLF GRILPEESKL FKELNRLENV 

      1090       1100       1110       1120       1130       1140 
QTSSLEDFMD DENATDRPMD DEQDLGMAIE HSEDMKGRIV VVQQYFKDLE NRHGISFLFN 

      1150       1160       1170       1180       1190       1200 
LIPDETFPKT KDRLHAKFGL GQKEFSKIKL SIGYSTEEGT VFRSLQGFSD EELDKVILYD 

      1210       1220       1230 
IMSNLDYIYM DHPDRLRSHS SYDRPMIIKN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49212 Genomic DNA. Translation: CAA89137.1.
BK006946 Genomic DNA. Translation: DAA10205.1.
PIRS53974.
RefSeqNP_014033.1. NM_001182814.1.

3D structure databases

ProteinModelPortalP50101.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35484. 115 interactions.
DIPDIP-6313N.
IntActP50101. 32 interactions.
MINTMINT-618224.
STRING4932.YMR304W.

Protein family/group databases

MEROPSC19.099.

Proteomic databases

PaxDbP50101.
PeptideAtlasP50101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR304W; YMR304W; YMR304W.
GeneID855350.
KEGGsce:YMR304W.

Organism-specific databases

CYGDYMR304w.
SGDS000004920. UBP15.

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00740000115066.
HOGENOMHOG000160240.
KOK11838.
OMAENFGANE.
OrthoDBEOG7C2R8P.

Enzyme and pathway databases

BioCycYEAST:G3O-32969-MONOMER.

Gene expression databases

GenevestigatorP50101.

Family and domain databases

InterProIPR002083. MATH.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR008974. TRAF-like.
IPR028889. UCH/PAN2.
IPR024729. USP7_ICP0-binding_dom.
[Graphical view]
PfamPF00917. MATH. 1 hit.
PF00443. UCH. 1 hit.
PF12436. USP7_ICP0_bdg. 1 hit.
[Graphical view]
SMARTSM00061. MATH. 1 hit.
[Graphical view]
SUPFAMSSF49599. SSF49599. 1 hit.
PROSITEPS50144. MATH. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979106.
PROP50101.

Entry information

Entry nameUBP15_YEAST
AccessionPrimary (citable) accession number: P50101
Secondary accession number(s): D6W0D1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries