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P50098

- IMDH_TRYBB

UniProt

P50098 - IMDH_TRYBB

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Protein

Inosine-5'-monophosphate dehydrogenase

Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth By similarity.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

Potassium By similarity.UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi320 – 3201Potassium; via carbonyl oxygen By similarity
Metal bindingi322 – 3221Potassium; via carbonyl oxygen By similarity
Binding sitei323 – 3231IMP By similarity
Active sitei325 – 3251Thioimidate intermediate By similarity
Metal bindingi325 – 3251Potassium; via carbonyl oxygen By similarity
Binding sitei435 – 4351IMP By similarity
Metal bindingi494 – 4941Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi495 – 4951Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2703NAD By similarity
Nucleotide bindingi318 – 3203NAD By similarity

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase (EC:1.1.1.205)
Short name:
IMP dehydrogenase
Short name:
IMPD
Short name:
IMPDH
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

Glycosome By similarity UniRule annotation

GO - Cellular componenti

  1. glycosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Inosine-5'-monophosphate dehydrogenaseUniRule annotationPRO_0000093677Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50098.
SMRiP50098. Positions 13-106.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini110 – 16960CBS 1Add
BLAST
Domaini173 – 23159CBS 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni358 – 3603IMP binding By similarity
Regioni381 – 3822IMP binding By similarity
Regioni405 – 4095IMP binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi510 – 5123Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50098-1 [UniParc]FASTAAdd to Basket

« Hide

MENTNLRTKT LRDGTTAEEL FSQDGLSFND FIILPGFIDF DSSKVNVSGQ    50
FTKNILLHLP LVSSPMDTVT ESSMARAMAL MGGIGVIHNN CTVEQQARMV 100
RSVKLYRNGF IMKPKSVSPD VPVSTIRNIK SEKGISGILV TEGGKYDGKL 150
LGIVCTKDID FVKDASAPVS QYMTRRENMT VERYPIKLEE AMDVLNRSRH 200
GYLPVLNDKD EVVCLCSRRD AVRARDYPNS SLDRNGHLLC AAATSTREAD 250
KGRVAALSEA GIDVLVLDSS QGNTIYQVSF IRWVKKTYPH LEVVAGNVVT 300
QDQAKNLIDA GADSLRIGMG SGSICITQEV LACGRPQATA IYKVARYAAS 350
RGVPCVADGG LRNVGDVCKA LAVGANVAML GSMIAGTSET PGEYFFKDGM 400
RLKGYRGMGS IDAMLQGRES GKRYLSENET LQVAQGVAGA VLDKGSVLKL 450
LAYIHKGLQQ SAQDIGEVSF DAIREKVYEG QVLFNRRTLT AQSEGAVHSL 500
HHYERKLFAS KL 512
Length:512
Mass (Da):55,709
Last modified:October 1, 1996 - v1
Checksum:i1A86C46AE6445045
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97794 Genomic DNA. Translation: AAB46420.1.
PIRiA55407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97794 Genomic DNA. Translation: AAB46420.1 .
PIRi A55407.

3D structure databases

ProteinModelPortali P50098.
SMRi P50098. Positions 13-106.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amplification of the inosinate dehydrogenase gene in Trypanosoma brucei gambiense due to an increase in chromosome copy number."
    Wilson K., Berens R.L., Sifri C.D., Ullman B.
    J. Biol. Chem. 269:28979-28987(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Eatro 164 / ISTat 1.7.

Entry informationi

Entry nameiIMDH_TRYBB
AccessioniPrimary (citable) accession number: P50098
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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