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P50094

- IMDH4_YEAST

UniProt

P50094 - IMDH4_YEAST

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Protein
Inosine-5'-monophosphate dehydrogenase 4
Gene
IMD4, YML056C, YM9958.06C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.2 Publications

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

Potassium By similarity.UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi331 – 3311Potassium; via carbonyl oxygen By similarity
Metal bindingi333 – 3331Potassium; via carbonyl oxygen By similarity
Binding sitei334 – 3341IMP By similarity
Active sitei336 – 3361Thioimidate intermediate By similarity
Metal bindingi336 – 3361Potassium; via carbonyl oxygen By similarity
Binding sitei450 – 4501IMP By similarity
Metal bindingi509 – 5091Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi510 – 5101Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi511 – 5111Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2813NAD By similarity
Nucleotide bindingi329 – 3313NAD By similarity

GO - Molecular functioni

  1. IMP dehydrogenase activity Source: SGD
  2. adenyl nucleotide binding Source: InterPro
  3. metal ion binding Source: UniProtKB-HAMAP
  4. protein binding Source: IntAct

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciYEAST:YML056C-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase 4 (EC:1.1.1.205)
Short name:
IMP dehydrogenase 4
Short name:
IMPD 4
Short name:
IMPDH 4
Gene namesi
Name:IMD4
Ordered Locus Names:YML056C
ORF Names:YM9958.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML056c.
SGDiS000004520. IMD4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Inosine-5'-monophosphate dehydrogenase 4UniRule annotation
PRO_0000093684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei125 – 1251Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50094.
PaxDbiP50094.
PeptideAtlasiP50094.

Expressioni

Gene expression databases

GenevestigatoriP50094.

Interactioni

Subunit structurei

Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IMD3P500956EBI-9195,EBI-9190

Protein-protein interaction databases

BioGridi35111. 85 interactions.
DIPiDIP-6458N.
IntActiP50094. 60 interactions.
MINTiMINT-675699.
STRINGi4932.YML056C.

Structurei

3D structure databases

ProteinModelPortaliP50094.
SMRiP50094. Positions 2-523.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 18362CBS 1
Add
BLAST
Domaini185 – 24157CBS 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni369 – 3713IMP binding By similarity
Regioni392 – 3932IMP binding By similarity
Regioni416 – 4205IMP binding By similarity

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
GeneTreeiENSGT00530000062923.
HOGENOMiHOG000165752.
KOiK00088.
OMAiYLIFLET.
OrthoDBiEOG793BHK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50094-1 [UniParc]FASTAAdd to Basket

« Hide

MSAAPLDYKK ALEHLKTYSS KDGLSVQELM DSTTRGGLTY NDFLVLPGLV    50
NFPSSAVSLQ TKLTKKITLN TPFVSSPMDT VTEADMAIYM ALLGGIGFIH 100
HNCTPKEQAS MVKKVKMFEN GFINSPIVIS PTTTVGEVKV MKRKFGFSGF 150
PVTEDGKCPG KLVGLVTSRD IQFLEDDSLV VSEVMTKNPV TGIKGITLKE 200
GNEILKQTKK GKLLIVDDNG NLVSMLSRAD LMKNQNYPLA SKSATTKQLL 250
CGAAIGTIEA DKERLRLLVE AGLDVVILDS SQGNSVFQLN MIKWIKETFP 300
DLEIIAGNVA TREQAANLIA AGADGLRIGM GSGSICITQE VMACGRPQGT 350
AVYNVCQFAN QFGVPCMADG GVQNIGHITK ALALGSSTVM MGGMLAGTTE 400
SPGEYFYKDG KRLKAYRGMG SIDAMQKTGN KGNASTSRYF SESDSVLVAQ 450
GVSGAVVDKG SIKKFIPYLY NGLQHSCQDI GCESLTSLKE NVQNGEVRFE 500
FRTASAQLEG GVHNLHSYEK RLYN 524
Length:524
Mass (Da):56,394
Last modified:October 1, 1996 - v1
Checksum:iA73D1E4EFE8AEAD9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46729 Genomic DNA. Translation: CAA86719.1.
AY899250 mRNA. Translation: AAX83935.1.
BK006946 Genomic DNA. Translation: DAA09842.1.
PIRiS50890.
RefSeqiNP_013656.1. NM_001182414.1.

Genome annotation databases

EnsemblFungiiYML056C; YML056C; YML056C.
GeneIDi854948.
KEGGisce:YML056C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46729 Genomic DNA. Translation: CAA86719.1 .
AY899250 mRNA. Translation: AAX83935.1 .
BK006946 Genomic DNA. Translation: DAA09842.1 .
PIRi S50890.
RefSeqi NP_013656.1. NM_001182414.1.

3D structure databases

ProteinModelPortali P50094.
SMRi P50094. Positions 2-523.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35111. 85 interactions.
DIPi DIP-6458N.
IntActi P50094. 60 interactions.
MINTi MINT-675699.
STRINGi 4932.YML056C.

Proteomic databases

MaxQBi P50094.
PaxDbi P50094.
PeptideAtlasi P50094.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML056C ; YML056C ; YML056C .
GeneIDi 854948.
KEGGi sce:YML056C.

Organism-specific databases

CYGDi YML056c.
SGDi S000004520. IMD4.

Phylogenomic databases

eggNOGi COG0517.
GeneTreei ENSGT00530000062923.
HOGENOMi HOG000165752.
KOi K00088.
OMAi YLIFLET.
OrthoDBi EOG793BHK.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci YEAST:YML056C-MONOMER.

Miscellaneous databases

NextBioi 978010.

Gene expression databases

Genevestigatori P50094.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
    Zhang Z., Dietrich F.S.
    Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-83.
    Strain: ATCC 208353 / W303-1A.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast."
    Hyle J.W., Shaw R.J., Reines D.
    J. Biol. Chem. 278:28470-28478(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Detection of the mycophenolate-inhibited form of IMP dehydrogenase in vivo."
    McPhillips C.C., Hyle J.W., Reines D.
    Proc. Natl. Acad. Sci. U.S.A. 101:12171-12176(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIMDH4_YEAST
AccessioniPrimary (citable) accession number: P50094
Secondary accession number(s): D6VZB8, Q2VQW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2970 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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