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P50091

- PEX21_YEAST

UniProt

P50091 - PEX21_YEAST

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Protein
Peroxisomal membrane protein PEX21
Gene
PEX21, YGR239C, G8593
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on oleate. Acts as an activator of the seryl-tRNA synthetase SES1 by increasing its binding to tRNA.4 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. positive regulation of protein binding Source: SGD
  2. protein import into peroxisome matrix Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30917-MONOMER.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal membrane protein PEX21
Alternative name(s):
Peroxin-21
Gene namesi
Name:PEX21
Ordered Locus Names:YGR239C
ORF Names:G8593
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR239c.
SGDiS000003471. PEX21.

Subcellular locationi

Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. peroxisomal membrane Source: UniProtKB-SubCell
  3. peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Peroxisomal membrane protein PEX21
PRO_0000202855Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP50091.

Interactioni

Subunit structurei

Interacts with PEX7, PEX13 and SES1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX7P391086EBI-23549,EBI-13183

Protein-protein interaction databases

BioGridi33491. 16 interactions.
DIPiDIP-1503N.
IntActiP50091. 6 interactions.
MINTiMINT-410398.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi198 – 20912
Helixi225 – 2328
Helixi235 – 24410
Beta strandi247 – 2504
Beta strandi262 – 2643
Turni265 – 2673
Beta strandi269 – 2724

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W15X-ray1.80B190-288[»]
ProteinModelPortaliP50091.
SMRiP50091. Positions 197-288.

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxin-21 family.

Phylogenomic databases

eggNOGiNOG70678.
OMAiEICIERT.
OrthoDBiEOG71GB7H.

Sequencei

Sequence statusi: Complete.

P50091-1 [UniParc]FASTAAdd to Basket

« Hide

MPSVCHTSPI EKIIQQGHRI QNDSLIPSKR TKLAHTELTA HYATEDSHVE    50
KHFLHNGSNF DGIDNVRYQN QPSPLTFITP NNTVDSSDWV PQFSSMKIDD 100
SLEFSSEYKR LYSNYESQQR LNSSRQHLPF KNCMIRKTSC TYPPQKTLRQ 150
QRQGNRDNPT DAFQFDAEFQ VLEREIQKER YEPITRRDEK WFDQDQSELQ 200
RIATDIVKCC TPPPSSASSS STLSSSVESK LSESKFIQLM RNISSGDVTL 250
KKNADGNSAS ELFSSNNGEL VGNRHIFVKD EIHKDILD 288
Length:288
Mass (Da):33,050
Last modified:October 1, 1996 - v1
Checksum:i0197257317ADE4FF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87941 Genomic DNA. Translation: CAA61191.1.
Z73024 Genomic DNA. Translation: CAA97267.1.
Z73025 Genomic DNA. Translation: CAA97269.1.
AY558277 Genomic DNA. Translation: AAS56603.1.
BK006941 Genomic DNA. Translation: DAA08330.1.
PIRiS57706.
RefSeqiNP_011755.3. NM_001181368.3.

Genome annotation databases

EnsemblFungiiYGR239C; YGR239C; YGR239C.
GeneIDi853154.
KEGGisce:YGR239C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87941 Genomic DNA. Translation: CAA61191.1 .
Z73024 Genomic DNA. Translation: CAA97267.1 .
Z73025 Genomic DNA. Translation: CAA97269.1 .
AY558277 Genomic DNA. Translation: AAS56603.1 .
BK006941 Genomic DNA. Translation: DAA08330.1 .
PIRi S57706.
RefSeqi NP_011755.3. NM_001181368.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3W15 X-ray 1.80 B 190-288 [» ]
ProteinModelPortali P50091.
SMRi P50091. Positions 197-288.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33491. 16 interactions.
DIPi DIP-1503N.
IntActi P50091. 6 interactions.
MINTi MINT-410398.

Protein family/group databases

TCDBi 3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR239C ; YGR239C ; YGR239C .
GeneIDi 853154.
KEGGi sce:YGR239C.

Organism-specific databases

CYGDi YGR239c.
SGDi S000003471. PEX21.

Phylogenomic databases

eggNOGi NOG70678.
OMAi EICIERT.
OrthoDBi EOG71GB7H.

Enzyme and pathway databases

BioCyci YEAST:G3O-30917-MONOMER.

Miscellaneous databases

NextBioi 973246.

Gene expression databases

Genevestigatori P50091.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
    van der Aart Q.J.M., Kleine K., Steensma H.Y.
    Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
    Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
    Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-288.
    Strain: ATCC 204508 / S288c.
  6. "Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway."
    Purdue P.E., Yang X., Lazarow P.B.
    J. Cell Biol. 143:1859-1869(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PEX7.
  7. "Identifying Pex21p as a protein that specifically interacts with yeast seryl-tRNA synthetase."
    Rocak S., Landeka I., Weygand-Durasevic I.
    FEMS Microbiol. Lett. 214:101-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SES1.
  8. "Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import."
    Stein K., Schell-Steven A., Erdmann R., Rottensteiner H.
    Mol. Cell. Biol. 22:6056-6069(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PEX7 AND PEX13.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNA(Ser)."
    Godinic V., Mocibob M., Rocak S., Ibba M., Weygand-Durasevic I.
    FEBS J. 274:2788-2799(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SES1.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPEX21_YEAST
AccessioniPrimary (citable) accession number: P50091
Secondary accession number(s): D6VV19, O11855, Q6Q597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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