Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50091

- PEX21_YEAST

UniProt

P50091 - PEX21_YEAST

Protein

Peroxisomal membrane protein PEX21

Gene

PEX21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on oleate. Acts as an activator of the seryl-tRNA synthetase SES1 by increasing its binding to tRNA.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. positive regulation of protein binding Source: SGD
    2. protein import into peroxisome matrix Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30917-MONOMER.

    Protein family/group databases

    TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal membrane protein PEX21
    Alternative name(s):
    Peroxin-21
    Gene namesi
    Name:PEX21
    Ordered Locus Names:YGR239C
    ORF Names:G8593
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR239c.
    SGDiS000003471. PEX21.

    Subcellular locationi

    Cytoplasm 1 Publication. Peroxisome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. peroxisomal membrane Source: UniProtKB-SubCell
    3. peroxisome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Peroxisomal membrane protein PEX21PRO_0000202855Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP50091.

    Interactioni

    Subunit structurei

    Interacts with PEX7, PEX13 and SES1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PEX7P391086EBI-23549,EBI-13183

    Protein-protein interaction databases

    BioGridi33491. 16 interactions.
    DIPiDIP-1503N.
    IntActiP50091. 6 interactions.
    MINTiMINT-410398.

    Structurei

    Secondary structure

    1
    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi198 – 20912
    Helixi225 – 2328
    Helixi235 – 24410
    Beta strandi247 – 2504
    Beta strandi262 – 2643
    Turni265 – 2673
    Beta strandi269 – 2724

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3W15X-ray1.80B190-288[»]
    ProteinModelPortaliP50091.
    SMRiP50091. Positions 197-288.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxin-21 family.Curated

    Phylogenomic databases

    eggNOGiNOG70678.
    OMAiEICIERT.
    OrthoDBiEOG71GB7H.

    Sequencei

    Sequence statusi: Complete.

    P50091-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSVCHTSPI EKIIQQGHRI QNDSLIPSKR TKLAHTELTA HYATEDSHVE    50
    KHFLHNGSNF DGIDNVRYQN QPSPLTFITP NNTVDSSDWV PQFSSMKIDD 100
    SLEFSSEYKR LYSNYESQQR LNSSRQHLPF KNCMIRKTSC TYPPQKTLRQ 150
    QRQGNRDNPT DAFQFDAEFQ VLEREIQKER YEPITRRDEK WFDQDQSELQ 200
    RIATDIVKCC TPPPSSASSS STLSSSVESK LSESKFIQLM RNISSGDVTL 250
    KKNADGNSAS ELFSSNNGEL VGNRHIFVKD EIHKDILD 288
    Length:288
    Mass (Da):33,050
    Last modified:October 1, 1996 - v1
    Checksum:i0197257317ADE4FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87941 Genomic DNA. Translation: CAA61191.1.
    Z73024 Genomic DNA. Translation: CAA97267.1.
    Z73025 Genomic DNA. Translation: CAA97269.1.
    AY558277 Genomic DNA. Translation: AAS56603.1.
    BK006941 Genomic DNA. Translation: DAA08330.1.
    PIRiS57706.
    RefSeqiNP_011755.3. NM_001181368.3.

    Genome annotation databases

    EnsemblFungiiYGR239C; YGR239C; YGR239C.
    GeneIDi853154.
    KEGGisce:YGR239C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87941 Genomic DNA. Translation: CAA61191.1 .
    Z73024 Genomic DNA. Translation: CAA97267.1 .
    Z73025 Genomic DNA. Translation: CAA97269.1 .
    AY558277 Genomic DNA. Translation: AAS56603.1 .
    BK006941 Genomic DNA. Translation: DAA08330.1 .
    PIRi S57706.
    RefSeqi NP_011755.3. NM_001181368.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3W15 X-ray 1.80 B 190-288 [» ]
    ProteinModelPortali P50091.
    SMRi P50091. Positions 197-288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33491. 16 interactions.
    DIPi DIP-1503N.
    IntActi P50091. 6 interactions.
    MINTi MINT-410398.

    Protein family/group databases

    TCDBi 3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR239C ; YGR239C ; YGR239C .
    GeneIDi 853154.
    KEGGi sce:YGR239C.

    Organism-specific databases

    CYGDi YGR239c.
    SGDi S000003471. PEX21.

    Phylogenomic databases

    eggNOGi NOG70678.
    OMAi EICIERT.
    OrthoDBi EOG71GB7H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30917-MONOMER.

    Miscellaneous databases

    NextBioi 973246.

    Gene expression databases

    Genevestigatori P50091.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
      van der Aart Q.J.M., Kleine K., Steensma H.Y.
      Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
      Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
      Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-288.
      Strain: ATCC 204508 / S288c.
    6. "Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway."
      Purdue P.E., Yang X., Lazarow P.B.
      J. Cell Biol. 143:1859-1869(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEX7.
    7. "Identifying Pex21p as a protein that specifically interacts with yeast seryl-tRNA synthetase."
      Rocak S., Landeka I., Weygand-Durasevic I.
      FEMS Microbiol. Lett. 214:101-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SES1.
    8. "Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import."
      Stein K., Schell-Steven A., Erdmann R., Rottensteiner H.
      Mol. Cell. Biol. 22:6056-6069(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEX7 AND PEX13.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNA(Ser)."
      Godinic V., Mocibob M., Rocak S., Ibba M., Weygand-Durasevic I.
      FEBS J. 274:2788-2799(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SES1.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPEX21_YEAST
    AccessioniPrimary (citable) accession number: P50091
    Secondary accession number(s): D6VV19, O11855, Q6Q597
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3