P50090 (KEL2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kelch repeat-containing protein 2

Gene names

Name:	KEL2
Ordered Locus Names:	YGR238C
ORF Names:	G8585

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with KEL1.
Miscellaneous	Present with 468 molecules/cell in log phase SD medium.
Sequence similarities	Contains 6 Kelch repeats.

Ontologies

Keywords
Domain	Coiled coil Kelch repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	conjugation with cellular fusion Inferred from genetic interaction Ref.5. Source: SGD negative regulation of exit from mitosis Inferred from physical interaction PubMed 12234925. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay Ref.5. Source: SGD cellular bud tip Inferred from direct assay Ref.5. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KEL1	P38853	4	EBI-9630,EBI-9619

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Kelch repeat-containing protein 2		PRO_0000119097
Regions
	Repeat	99 – 143	45	Kelch 1
	Repeat	153 – 207	55	Kelch 2
	Repeat	213 – 267	55	Kelch 3
	Repeat	268 – 317	50	Kelch 4
	Repeat	319 – 369	51	Kelch 5
	Repeat	371 – 417	47	Kelch 6
	Coiled coil	550 – 685	136	Potential
	Coiled coil	728 – 881	154	Potential
Amino acid modifications
	Modified residue	455	1	Phosphothreonine Ref.7
	Modified residue	465	1	Phosphoserine Ref.7
	Modified residue	509	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P50090 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 63B9772FF017E6D9	FASTA	882	99,975
	10 20 30 40 50 60 MVPFKLTNKV PTDTGPSLIS AQSVPRPIVF MDNRNNTRIV TPTLPPNQHR GISGASTALP 70 80 90 100 110 120 WSPESKNTGK YIWNRVKLKN SPFPRYRHSS SFIVTNDNRI FVTGGLHDQS VYGDVWQIAA 130 140 150 160 170 180 NADGTSFTSK RIDIDQNTPP PRVGHASTIC GNAYVVFGGD THKLNKNGLL DDDLYLFNIN 190 200 210 220 230 240 SYKWTIPQPI GRRPLGRYGH KISIIASNPM QTKLYLFGGQ VDETYFNDLV VFDLSSFRRP 250 260 270 280 290 300 NSHWEFLEPV GDLPPPLTNH TMVAYDNKLW VFGGETPKTI SNDTYRYDPA QSEWSKVKTT 310 320 330 340 350 360 GEKPPPIQEH ASVVYKHLMC VLGGKDTHNA YSNDVYFLNL LSLKWYKLPR MKEGIPQERS 370 380 390 400 410 420 GHSLTLMKNE KLLIMGGDKT DYASPNIHDL QTSETDQGEG TLLYTLDLSS LNELCPGIMC 430 440 450 460 470 480 ESLHAGESFS NSLSGGFTPS KSTESENQEI INILTPRLPD SKVLSYNDID EGAGSYSSAL 490 500 510 520 530 540 DDKAFERKSD REEKKPQSSK VDSSINKESP GTGIKVSKKN FPVLRGLTVD SEEYGSSSYK 550 560 570 580 590 600 DTSCQKGIPK NLFDDLNLNL QTLRLEAQQK ELETARHISQ LEKEVQRLMV IKEASKDSNF 610 620 630 640 650 660 QTARLKNLEI QKTFLESRIN DLKNLLMVKL SQASKLCDQI TIQNNGLKTC SEHVTIKRDI 670 680 690 700 710 720 IDLENKCDVL KRQNEILVNN MQKITPELHT YLNESSCYLG KLLKSYPTSA RPPSSEKDNQ 730 740 750 760 770 780 IYEKDSLNKI EKVINEMHET VRAKEKLHLE TQKLNDERDS LRANLLDNNN KLDALRKLSD 790 800 810 820 830 840 GSSKSMDLTK KAIHLSQSEL EKYRKNNDDL QKEIDRIKTE QAEQDDKQEQ RGAITHGNFD 850 860 870 880 AFHRMKINNL KAELYMSKEN RDSLKDELLA LKKKLYTLEQ KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Identification of Kel1p, a kelch domain-containing protein involved in cell fusion and morphology in Saccharomyces cerevisiae." Philips J., Herskowitz I. J. Cell Biol. 143:375-389(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-455; SER-465 AND SER-509, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87941 Genomic DNA. Translation: CAA61189.1. Z73023 Genomic DNA. Translation: CAA97266.1. AY693035 Genomic DNA. Translation: AAT93054.1. BK006941 Genomic DNA. Translation: DAA08329.1.
PIR	S57704.
RefSeq	NP_011754.3. NM_001181367.3.
3D structure databases
ProteinModelPortal	P50090.
SMR	P50090. Positions 845-876.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4229N.
IntAct	P50090. 9 interactions.
MINT	MINT-542042.
STRING	4932.YGR238C.
Proteomic databases
PaxDb	P50090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR238C; YGR238C; YGR238C.
GeneID	853153.
KEGG	sce:YGR238C. sce:YGR244C.
Organism-specific databases
CYGD	YGR238c.
SGD	S000003470. KEL2.
Phylogenomic databases
eggNOG	NOG145020.
GeneTree	ENSGT00700000104199.
HOGENOM	HOG000000942.
KO	K01900.
OMA	GHASTIC.
OrthoDB	EOG49W5PF.
Gene expression databases
Genevestigator	P50090.
GermOnline	YGR238C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.120.10.80. 2 hits.
InterPro	IPR015915. Kelch-typ_b-propeller. [Graphical view]
ProtoNet	Search...
Other
NextBio	973243.

Entry information

Entry name

KEL2_YEAST

Accession

Primary (citable) accession number: P50090
Secondary accession number(s): D6VV18

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program