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Protein

Probable 26S proteasome regulatory subunit p28

Gene

NAS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.3 Publications

GO - Biological processi

  • proteasome regulatory particle assembly Source: SGD
  • proteolysis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30910-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-382556. ABC-family proteins mediated transport.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 26S proteasome regulatory subunit p28
Alternative name(s):
Proteasome non-ATPase subunit 6
Gene namesi
Name:NAS6
Ordered Locus Names:YGR232W
ORF Names:G8564
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR232W.
SGDiS000003464. NAS6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670481 – 228Probable 26S proteasome regulatory subunit p28Add BLAST228

Proteomic databases

MaxQBiP50086.
PRIDEiP50086.

PTM databases

iPTMnetiP50086.

Interactioni

Subunit structurei

Interacts with RPT3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HSM3P383486EBI-14028,EBI-21152
RPN14P531964EBI-14028,EBI-23691
RPT3P332989EBI-14028,EBI-13905

Protein-protein interaction databases

BioGridi33484. 43 interactors.
DIPiDIP-1591N.
IntActiP50086. 26 interactors.
MINTiMINT-410562.

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 11Combined sources7
Helixi15 – 24Combined sources10
Helixi26 – 28Combined sources3
Helixi39 – 45Combined sources7
Helixi49 – 57Combined sources9
Helixi64 – 66Combined sources3
Helixi75 – 82Combined sources8
Helixi85 – 92Combined sources8
Beta strandi93 – 96Combined sources4
Helixi110 – 116Combined sources7
Helixi120 – 128Combined sources9
Helixi143 – 149Combined sources7
Helixi153 – 160Combined sources8
Turni161 – 163Combined sources3
Helixi177 – 183Combined sources7
Helixi187 – 197Combined sources11
Helixi211 – 214Combined sources4
Helixi220 – 225Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IXVX-ray2.30A1-228[»]
1WG0X-ray2.53A1-228[»]
2DZNX-ray2.20A/C/E1-228[»]
2DZOX-ray3.00A/C1-228[»]
ProteinModelPortaliP50086.
SMRiP50086.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50086.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 30ANK 1Add BLAST30
Repeati35 – 64ANK 2Add BLAST30
Repeati71 – 100ANK 3Add BLAST30
Repeati106 – 135ANK 4Add BLAST30
Repeati139 – 168ANK 5Add BLAST30
Repeati173 – 203ANK 6Add BLAST31

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00860000133720.
HOGENOMiHOG000158359.
InParanoidiP50086.
KOiK06694.
OMAiSCRVKDK.
OrthoDBiEOG092C5071.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYPLHQAC MENEFFKVQE LLHSKPSLLL QKDQDGRIPL HWSVSFQAHE
60 70 80 90 100
ITSFLLSKME NVNLDDYPDD SGWTPFHIAC SVGNLEVVKS LYDRPLKPDL
110 120 130 140 150
NKITNQGVTC LHLAVGKKWF EVSQFLIENG ASVRIKDKFN QIPLHRAASV
160 170 180 190 200
GSLKLIELLC GLGKSAVNWQ DKQGWTPLFH ALAEGHGDAA VLLVEKYGAE
210 220
YDLVDNKGAK AEDVALNEQV KKFFLNNV
Length:228
Mass (Da):25,616
Last modified:October 1, 1996 - v1
Checksum:i6B30C0DD034A6289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61182.1.
Z73017 Genomic DNA. Translation: CAA97260.1.
AY558275 Genomic DNA. Translation: AAS56601.1.
BK006941 Genomic DNA. Translation: DAA08323.1.
PIRiS57697.
RefSeqiNP_011748.3. NM_001181361.3.

Genome annotation databases

EnsemblFungiiYGR232W; YGR232W; YGR232W.
GeneIDi853147.
KEGGisce:YGR232W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61182.1.
Z73017 Genomic DNA. Translation: CAA97260.1.
AY558275 Genomic DNA. Translation: AAS56601.1.
BK006941 Genomic DNA. Translation: DAA08323.1.
PIRiS57697.
RefSeqiNP_011748.3. NM_001181361.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IXVX-ray2.30A1-228[»]
1WG0X-ray2.53A1-228[»]
2DZNX-ray2.20A/C/E1-228[»]
2DZOX-ray3.00A/C1-228[»]
ProteinModelPortaliP50086.
SMRiP50086.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33484. 43 interactors.
DIPiDIP-1591N.
IntActiP50086. 26 interactors.
MINTiMINT-410562.

PTM databases

iPTMnetiP50086.

Proteomic databases

MaxQBiP50086.
PRIDEiP50086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR232W; YGR232W; YGR232W.
GeneIDi853147.
KEGGisce:YGR232W.

Organism-specific databases

EuPathDBiFungiDB:YGR232W.
SGDiS000003464. NAS6.

Phylogenomic databases

GeneTreeiENSGT00860000133720.
HOGENOMiHOG000158359.
InParanoidiP50086.
KOiK06694.
OMAiSCRVKDK.
OrthoDBiEOG092C5071.

Enzyme and pathway databases

BioCyciYEAST:G3O-30910-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-382556. ABC-family proteins mediated transport.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiP50086.
PROiP50086.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSD10_YEAST
AccessioniPrimary (citable) accession number: P50086
Secondary accession number(s): D6VV12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.