P50086 (PSD10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 26S proteasome regulatory subunit p28

Alternative name(s):
Proteasome non-ATPase subunit 6

Gene names

Name:	NAS6
Ordered Locus Names:	YGR232W
ORF Names:	G8564

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14. Ref.5 Ref.7 Ref.8
Subunit structure	Interacts with RPT3. Ref.8
Miscellaneous	Present with 319 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 6 ANK repeats.

Ontologies

Keywords
Domain	ANK repeat Repeat
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteasome regulatory particle assembly Inferred from mutant phenotype Ref.7. Source: SGD proteolysis Inferred from physical interaction. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSM3	P38348	6	EBI-14028,EBI-21152
RPN14	P53196	5	EBI-14028,EBI-23691
RPT3	P33298	9	EBI-14028,EBI-13905

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 228

228

Probable 26S proteasome regulatory subunit p28

PRO_0000067048

Regions

Repeat

1 – 30

30

ANK 1

Repeat

35 – 64

30

ANK 2

Repeat

71 – 100

30

ANK 3

Repeat

106 – 135

30

ANK 4

Repeat

139 – 168

30

ANK 5

Repeat

173 – 203

31

ANK 6

Secondary structure

1

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228

Helix Strand Turn

Details...

Helix

5 – 12

8

Helix

15 – 24

10

Helix

26 – 28

3

Helix

39 – 45

7

Helix

49 – 56

8

Helix

64 – 66

3

Helix

75 – 82

8

Helix

85 – 92

8

Beta strand

93 – 96

4

Helix

110 – 116

7

Helix

120 – 128

9

Helix

143 – 150

8

Helix

153 – 160

8

Turn

161 – 163

3

Helix

177 – 183

7

Helix

187 – 197

11

Helix

212 – 214

3

Helix

218 – 227

10

Sequences

	Sequence		Length	Mass (Da)
	P50086 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6B30C0DD034A6289	FASTA	228	25,616
	10 20 30 40 50 60 MSNYPLHQAC MENEFFKVQE LLHSKPSLLL QKDQDGRIPL HWSVSFQAHE ITSFLLSKME 70 80 90 100 110 120 NVNLDDYPDD SGWTPFHIAC SVGNLEVVKS LYDRPLKPDL NKITNQGVTC LHLAVGKKWF 130 140 150 160 170 180 EVSQFLIENG ASVRIKDKFN QIPLHRAASV GSLKLIELLC GLGKSAVNWQ DKQGWTPLFH 190 200 210 220 ALAEGHGDAA VLLVEKYGAE YDLVDNKGAK AEDVALNEQV KKFFLNNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed: 8701610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome." Hori T., Kato S., Saeki M., DeMartino G.N., Slaughter C.A., Takeuchi J., Toh-e A., Tanaka K. Gene 216:113-122(1998) [PubMed: 9714768] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base." Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M. Cell 137:887-899(2009) [PubMed: 19446322] [Abstract] Cited for: FUNCTION AS PROTEASOME CHAPERONE.
[8]	"Chaperone-mediated pathway of proteasome regulatory particle assembly." Roelofs J., Park S., Haas W., Tian G., McAllister F.E., Huo Y., Lee B.H., Zhang F., Shi Y., Gygi S.P., Finley D. Nature 459:861-865(2009) [PubMed: 19412159] [Abstract] Cited for: FUNCTION AS PROTEASOME CHAPERONE, INTERACTION WITH RPT3.
[9]	"Crystal structure of the homolog of the oncoprotein gankyrin, an interactor of Rb and CDK4/6." Padmanabhan B., Adachi N., Kataoka K., Horikoshi M. J. Biol. Chem. 279:1546-1552(2004) [PubMed: 14583612] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[10]	"Structural comparison of NAS6p protein structures in two different crystal forms." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X87941 Genomic DNA. Translation: CAA61182.1.
Z73017 Genomic DNA. Translation: CAA97260.1.
AY558275 Genomic DNA. Translation: AAS56601.1.
BK006941 Genomic DNA. Translation: DAA08323.1.

PIR

S57697.

RefSeq

NP_011748.1. NM_001181361.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IXV	X-ray	2.30	A	1-228	[»]
1WG0	X-ray	2.53	A	1-228	[»]
2DZN	X-ray	2.20	A/C/E	1-228	[»]
2DZO	X-ray	3.00	A/C	1-228	[»]

ProteinModelPortal

P50086.

SMR

P50086. Positions 2-228.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1591N.

IntAct

P50086. 28 interactions.

MINT

MINT-410562.

STRING

P50086.

Proteomic databases

PeptideAtlas

P50086.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR232W; YGR232W; YGR232W.

GeneID

853147.

KEGG

sce:YGR232W.

NMPDR

fig|4932.3.peg.2876.

Organism-specific databases

CYGD

YGR232w.

SGD

S000003464. NAS6.

Phylogenomic databases

eggNOG

fuNOG09268.

GeneTree

EFGT00070000008731.

HOGENOM

HBG595165.

OMA

HRGADIG.

OrthoDB

EOG42Z808.

Gene expression databases

ArrayExpress

P50086.

Genevestigator

P50086.

GermOnline

YGR232W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]

Gene3D

G3DSA:1.25.40.20. ANK. 1 hit.

KO

K06694.

Pfam

PF00023. Ank. 2 hits.
[Graphical view]

PRINTS

PR01415. ANKYRIN.

SMART

SM00248. ANK. 6 hits.
[Graphical view]

SUPFAM

SSF48403. ANK. 1 hit.

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

973225.

Entry information

Entry name

PSD10_YEAST

Accession

Primary (citable) accession number: P50086
Secondary accession number(s): D6VV12

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program