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Protein

Probable 26S proteasome regulatory subunit p28

Gene

NAS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.3 Publications

GO - Biological processi

  • proteasome regulatory particle assembly Source: SGD
  • proteolysis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30910-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 26S proteasome regulatory subunit p28
Alternative name(s):
Proteasome non-ATPase subunit 6
Gene namesi
Name:NAS6
Ordered Locus Names:YGR232W
ORF Names:G8564
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR232W.
SGDiS000003464. NAS6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Probable 26S proteasome regulatory subunit p28PRO_0000067048Add
BLAST

Proteomic databases

MaxQBiP50086.
PeptideAtlasiP50086.

PTM databases

iPTMnetiP50086.

Interactioni

Subunit structurei

Interacts with RPT3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HSM3P383486EBI-14028,EBI-21152
RPN14P531964EBI-14028,EBI-23691
RPT3P332989EBI-14028,EBI-13905

Protein-protein interaction databases

BioGridi33484. 43 interactions.
DIPiDIP-1591N.
IntActiP50086. 26 interactions.
MINTiMINT-410562.

Structurei

Secondary structure

1
228
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 117Combined sources
Helixi15 – 2410Combined sources
Helixi26 – 283Combined sources
Helixi39 – 457Combined sources
Helixi49 – 579Combined sources
Helixi64 – 663Combined sources
Helixi75 – 828Combined sources
Helixi85 – 928Combined sources
Beta strandi93 – 964Combined sources
Helixi110 – 1167Combined sources
Helixi120 – 1289Combined sources
Helixi143 – 1497Combined sources
Helixi153 – 1608Combined sources
Turni161 – 1633Combined sources
Helixi177 – 1837Combined sources
Helixi187 – 19711Combined sources
Helixi211 – 2144Combined sources
Helixi220 – 2256Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IXVX-ray2.30A1-228[»]
1WG0X-ray2.53A1-228[»]
2DZNX-ray2.20A/C/E1-228[»]
2DZOX-ray3.00A/C1-228[»]
ProteinModelPortaliP50086.
SMRiP50086. Positions 2-228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50086.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 3030ANK 1Add
BLAST
Repeati35 – 6430ANK 2Add
BLAST
Repeati71 – 10030ANK 3Add
BLAST
Repeati106 – 13530ANK 4Add
BLAST
Repeati139 – 16830ANK 5Add
BLAST
Repeati173 – 20331ANK 6Add
BLAST

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00840000129743.
HOGENOMiHOG000158359.
InParanoidiP50086.
KOiK06694.
OMAiIQNKEKK.
OrthoDBiEOG7KSXM5.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYPLHQAC MENEFFKVQE LLHSKPSLLL QKDQDGRIPL HWSVSFQAHE
60 70 80 90 100
ITSFLLSKME NVNLDDYPDD SGWTPFHIAC SVGNLEVVKS LYDRPLKPDL
110 120 130 140 150
NKITNQGVTC LHLAVGKKWF EVSQFLIENG ASVRIKDKFN QIPLHRAASV
160 170 180 190 200
GSLKLIELLC GLGKSAVNWQ DKQGWTPLFH ALAEGHGDAA VLLVEKYGAE
210 220
YDLVDNKGAK AEDVALNEQV KKFFLNNV
Length:228
Mass (Da):25,616
Last modified:October 1, 1996 - v1
Checksum:i6B30C0DD034A6289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61182.1.
Z73017 Genomic DNA. Translation: CAA97260.1.
AY558275 Genomic DNA. Translation: AAS56601.1.
BK006941 Genomic DNA. Translation: DAA08323.1.
PIRiS57697.
RefSeqiNP_011748.3. NM_001181361.3.

Genome annotation databases

EnsemblFungiiYGR232W; YGR232W; YGR232W.
GeneIDi853147.
KEGGisce:YGR232W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61182.1.
Z73017 Genomic DNA. Translation: CAA97260.1.
AY558275 Genomic DNA. Translation: AAS56601.1.
BK006941 Genomic DNA. Translation: DAA08323.1.
PIRiS57697.
RefSeqiNP_011748.3. NM_001181361.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IXVX-ray2.30A1-228[»]
1WG0X-ray2.53A1-228[»]
2DZNX-ray2.20A/C/E1-228[»]
2DZOX-ray3.00A/C1-228[»]
ProteinModelPortaliP50086.
SMRiP50086. Positions 2-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33484. 43 interactions.
DIPiDIP-1591N.
IntActiP50086. 26 interactions.
MINTiMINT-410562.

PTM databases

iPTMnetiP50086.

Proteomic databases

MaxQBiP50086.
PeptideAtlasiP50086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR232W; YGR232W; YGR232W.
GeneIDi853147.
KEGGisce:YGR232W.

Organism-specific databases

EuPathDBiFungiDB:YGR232W.
SGDiS000003464. NAS6.

Phylogenomic databases

GeneTreeiENSGT00840000129743.
HOGENOMiHOG000158359.
InParanoidiP50086.
KOiK06694.
OMAiIQNKEKK.
OrthoDBiEOG7KSXM5.

Enzyme and pathway databases

BioCyciYEAST:G3O-30910-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiP50086.
PROiP50086.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
    van der Aart Q.J.M., Kleine K., Steensma H.Y.
    Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome."
    Hori T., Kato S., Saeki M., DeMartino G.N., Slaughter C.A., Takeuchi J., Toh-e A., Tanaka K.
    Gene 216:113-122(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base."
    Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M.
    Cell 137:887-899(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PROTEASOME CHAPERONE.
  8. "Chaperone-mediated pathway of proteasome regulatory particle assembly."
    Roelofs J., Park S., Haas W., Tian G., McAllister F.E., Huo Y., Lee B.H., Zhang F., Shi Y., Gygi S.P., Finley D.
    Nature 459:861-865(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PROTEASOME CHAPERONE, INTERACTION WITH RPT3.
  9. "Crystal structure of the homolog of the oncoprotein gankyrin, an interactor of Rb and CDK4/6."
    Padmanabhan B., Adachi N., Kataoka K., Horikoshi M.
    J. Biol. Chem. 279:1546-1552(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  10. "Structural comparison of NAS6p protein structures in two different crystal forms."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS).

Entry informationi

Entry nameiPSD10_YEAST
AccessioniPrimary (citable) accession number: P50086
Secondary accession number(s): D6VV12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.