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Protein

Probable 26S proteasome regulatory subunit p28

Gene

NAS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.3 Publications

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • proteasome regulatory particle assembly Source: SGD
  • proteolysis Source: SGD

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30910-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-187577 SCF(Skp2)-mediated degradation of p27/p21
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 26S proteasome regulatory subunit p28
Alternative name(s):
Proteasome non-ATPase subunit 6
Gene namesi
Name:NAS6
Ordered Locus Names:YGR232W
ORF Names:G8564
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR232W
SGDiS000003464 NAS6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670481 – 228Probable 26S proteasome regulatory subunit p28Add BLAST228

Proteomic databases

MaxQBiP50086
PaxDbiP50086
PRIDEiP50086

PTM databases

CarbonylDBiP50086
iPTMnetiP50086

Interactioni

Subunit structurei

Interacts with RPT3.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33484, 71 interactors
DIPiDIP-1591N
IntActiP50086, 29 interactors
MINTiP50086
STRINGi4932.YGR232W

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 11Combined sources7
Helixi15 – 24Combined sources10
Helixi26 – 28Combined sources3
Helixi39 – 45Combined sources7
Helixi49 – 57Combined sources9
Helixi64 – 66Combined sources3
Helixi75 – 82Combined sources8
Helixi85 – 92Combined sources8
Beta strandi93 – 96Combined sources4
Helixi110 – 116Combined sources7
Helixi120 – 128Combined sources9
Helixi143 – 149Combined sources7
Helixi153 – 160Combined sources8
Turni161 – 163Combined sources3
Helixi177 – 183Combined sources7
Helixi187 – 197Combined sources11
Helixi211 – 214Combined sources4
Helixi220 – 225Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IXVX-ray2.30A1-228[»]
1WG0X-ray2.53A1-228[»]
2DZNX-ray2.20A/C/E1-228[»]
2DZOX-ray3.00A/C1-228[»]
ProteinModelPortaliP50086
SMRiP50086
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50086

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 30ANK 1Add BLAST30
Repeati35 – 64ANK 2Add BLAST30
Repeati71 – 100ANK 3Add BLAST30
Repeati106 – 135ANK 4Add BLAST30
Repeati139 – 168ANK 5Add BLAST30
Repeati173 – 203ANK 6Add BLAST31

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00910000144039
HOGENOMiHOG000158359
InParanoidiP50086
KOiK06694
OMAiSVNQNGC
OrthoDBiEOG092C5071

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits

Sequencei

Sequence statusi: Complete.

P50086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYPLHQAC MENEFFKVQE LLHSKPSLLL QKDQDGRIPL HWSVSFQAHE
60 70 80 90 100
ITSFLLSKME NVNLDDYPDD SGWTPFHIAC SVGNLEVVKS LYDRPLKPDL
110 120 130 140 150
NKITNQGVTC LHLAVGKKWF EVSQFLIENG ASVRIKDKFN QIPLHRAASV
160 170 180 190 200
GSLKLIELLC GLGKSAVNWQ DKQGWTPLFH ALAEGHGDAA VLLVEKYGAE
210 220
YDLVDNKGAK AEDVALNEQV KKFFLNNV
Length:228
Mass (Da):25,616
Last modified:October 1, 1996 - v1
Checksum:i6B30C0DD034A6289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA Translation: CAA61182.1
Z73017 Genomic DNA Translation: CAA97260.1
AY558275 Genomic DNA Translation: AAS56601.1
BK006941 Genomic DNA Translation: DAA08323.1
PIRiS57697
RefSeqiNP_011748.3, NM_001181361.3

Genome annotation databases

EnsemblFungiiYGR232W; YGR232W; YGR232W
GeneIDi853147
KEGGisce:YGR232W

Similar proteinsi

Entry informationi

Entry nameiPSD10_YEAST
AccessioniPrimary (citable) accession number: P50086
Secondary accession number(s): D6VV12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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