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Protein

Prohibitin-2

Gene

PHB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins.1 Publication

Miscellaneous

Present with 2850 molecules/cell in log phase SD medium.1 Publication
Mitochondrial targeting of PHB2 is ensured by a bipartite non-cleavable presequence at the N-terminus.

GO - Biological processi

  • mitochondrion inheritance Source: SGD
  • negative regulation of proteolysis Source: SGD
  • protein folding Source: SGD
  • replicative cell aging Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30909-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-2
Gene namesi
Name:PHB2
Ordered Locus Names:YGR231C
ORF Names:G8561
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR231C.
SGDiS000003463. PHB2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei38 – 58Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138881 – 310Prohibitin-2Add BLAST310

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP50085.
PRIDEiP50085.

Interactioni

Subunit structurei

The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHB1P409613EBI-23530,EBI-13360

Protein-protein interaction databases

BioGridi33483. 419 interactors.
IntActiP50085. 43 interactors.
MINTiMINT-2734711.
STRINGi4932.YGR231C.

Structurei

3D structure databases

ProteinModelPortaliP50085.
SMRiP50085.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili212 – 2531 PublicationAdd BLAST42

Sequence similaritiesi

Belongs to the prohibitin family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075076.
HOGENOMiHOG000205692.
InParanoidiP50085.
KOiK17081.
OMAiELGTDYD.
OrthoDBiEOG092C43I2.

Family and domain databases

CDDicd03401. SPFH_prohibitin. 1 hit.
InterProiView protein in InterPro
IPR001107. Band_7.
IPR000163. Prohibitin.
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiView protein in Pfam
PF01145. Band_7. 1 hit.
PRINTSiPR00679. PROHIBITIN.
SMARTiView protein in SMART
SM00244. PHB. 1 hit.
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequencei

Sequence statusi: Complete.

P50085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRSPGEFQR YAKAFQKQLS KVQQTGGRGQ VPSPRGAFAG LGGLLLLGGG
60 70 80 90 100
ALFINNALFN VDGGHRAIVY SRIHGVSSRI FNEGTHFIFP WLDTPIIYDV
110 120 130 140 150
RAKPRNVASL TGTKDLQMVN ITCRVLSRPD VVQLPTIYRT LGQDYDERVL
160 170 180 190 200
PSIVNEVLKA VVAQFNASQL ITQREKVSRL IRENLVRRAS KFNILLDDVS
210 220 230 240 250
ITYMTFSPEF TNAVEAKQIA QQDAQRAAFV VDKARQEKQG MVVRAQGEAK
260 270 280 290 300
SAELIGEAIK KSRDYVELKR LDTARDIAKI LASSPNRVIL DNEALLLNTV
310
VDARIDGRGK
Length:310
Mass (Da):34,407
Last modified:October 3, 2006 - v2
Checksum:i3526D9690F39B493
GO

Sequence cautioni

The sequence CAA61181 differs from that shown. Reason: Frameshift at position 310.Curated
The sequence CAA97259 differs from that shown. Reason: Frameshift at position 310.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61181.1. Frameshift.
Z73016 Genomic DNA. Translation: CAA97259.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08322.1.
PIRiS57696.
RefSeqiNP_011747.2. NM_001181360.1.

Genome annotation databases

EnsemblFungiiYGR231C; YGR231C; YGR231C.
GeneIDi853146.
KEGGisce:YGR231C.

Similar proteinsi

Entry informationi

Entry nameiPHB2_YEAST
AccessioniPrimary (citable) accession number: P50085
Secondary accession number(s): D6VV11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: September 27, 2017
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names