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P50085 (PHB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prohibitin-2
Gene names
Name:PHB2
Ordered Locus Names:YGR231C
ORF Names:G8561
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Ref.4

Subunit structure

The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. Ref.4 Ref.5

Subcellular location

Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side Ref.7 Ref.9 Ref.10.

Post-translational modification

The N-terminus is blocked.

Miscellaneous

Present with 2850 molecules/cell in log phase SD medium.

Mitochondrial targeting of PHB2 is ensured by a bipartite non-cleavable presequence at the N-terminus.

Sequence similarities

Belongs to the prohibitin family.

Sequence caution

The sequence CAA61181.1 differs from that shown. Reason: Frameshift at position 310.

The sequence CAA97259.1 differs from that shown. Reason: Frameshift at position 310.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PHB1P409613EBI-23530,EBI-13360

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Prohibitin-2
PRO_0000213888

Regions

Transmembrane38 – 5821Helical; Signal-anchor for type II membrane protein; Potential
Coiled coil212 – 25342 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P50085 [UniParc].

Last modified October 3, 2006. Version 2.
Checksum: 3526D9690F39B493

FASTA31034,407
        10         20         30         40         50         60 
MNRSPGEFQR YAKAFQKQLS KVQQTGGRGQ VPSPRGAFAG LGGLLLLGGG ALFINNALFN 

        70         80         90        100        110        120 
VDGGHRAIVY SRIHGVSSRI FNEGTHFIFP WLDTPIIYDV RAKPRNVASL TGTKDLQMVN 

       130        140        150        160        170        180 
ITCRVLSRPD VVQLPTIYRT LGQDYDERVL PSIVNEVLKA VVAQFNASQL ITQREKVSRL 

       190        200        210        220        230        240 
IRENLVRRAS KFNILLDDVS ITYMTFSPEF TNAVEAKQIA QQDAQRAAFV VDKARQEKQG 

       250        260        270        280        290        300 
MVVRAQGEAK SAELIGEAIK KSRDYVELKR LDTARDIAKI LASSPNRVIL DNEALLLNTV 

       310 
VDARIDGRGK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
van der Aart Q.J.M., Kleine K., Steensma H.Y.
Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins."
Nijtmans L.G.J., de Jong L., Artal-Sanz M., Coates P.J., Berden J.A., Back J.W., Muijsers A.O., van der Spek H., Grivell L.A.
EMBO J. 19:2444-2451(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[5]"A structure for the yeast prohibitin complex: structure prediction and evidence from chemical crosslinking and mass spectrometry."
Back J.W., Artal-Sanz M., De Jong L., De Koning L.J., Nijtmans L.G.J., De Koster C.G., Grivell L.A., Van Der Spek H., Muijsers A.O.
Protein Sci. 11:2471-2478(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[6]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[10]"Formation of membrane-bound ring complexes by prohibitins in mitochondria."
Tatsuta T., Model K., Langer T.
Mol. Biol. Cell 16:248-259(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, SINGLE PARTICLE ELECTRON MICROSCOPY, COILED-COIL DOMAIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87941 Genomic DNA. Translation: CAA61181.1. Frameshift.
Z73016 Genomic DNA. Translation: CAA97259.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08322.1.
PIRS57696.
RefSeqNP_011747.2. NM_001181360.1.

3D structure databases

ProteinModelPortalP50085.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33483. 164 interactions.
IntActP50085. 41 interactions.
MINTMINT-2734711.
STRING4932.YGR231C.

Proteomic databases

PaxDbP50085.
PeptideAtlasP50085.
PRIDEP50085.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR231C; YGR231C; YGR231C.
GeneID853146.
KEGGsce:YGR231C.

Organism-specific databases

CYGDYGR231c.
SGDS000003463. PHB2.

Phylogenomic databases

eggNOGCOG0330.
GeneTreeENSGT00550000075076.
HOGENOMHOG000205692.
KOK17081.
OMATYMTFSP.
OrthoDBEOG7BS4MS.

Enzyme and pathway databases

BioCycYEAST:G3O-30909-MONOMER.

Gene expression databases

GenevestigatorP50085.

Family and domain databases

InterProIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERPTHR23222. PTHR23222. 1 hit.
PfamPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSPR00679. PROHIBITIN.
SMARTSM00244. PHB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973222.
PROP50085.

Entry information

Entry namePHB2_YEAST
AccessionPrimary (citable) accession number: P50085
Secondary accession number(s): D6VV11
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families