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Protein

Prohibitin-2

Gene

PHB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins.1 Publication

GO - Biological processi

  • mitochondrion inheritance Source: SGD
  • negative regulation of proteolysis Source: SGD
  • protein folding Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30909-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-2
Gene namesi
Name:PHB2
Ordered Locus Names:YGR231C
ORF Names:G8561
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR231c.
EuPathDBiFungiDB:YGR231C.
SGDiS000003463. PHB2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 5821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Prohibitin-2PRO_0000213888Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP50085.
PaxDbiP50085.
PeptideAtlasiP50085.
PRIDEiP50085.

Expressioni

Gene expression databases

GenevestigatoriP50085.

Interactioni

Subunit structurei

The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHB1P409613EBI-23530,EBI-13360

Protein-protein interaction databases

BioGridi33483. 165 interactions.
IntActiP50085. 42 interactions.
MINTiMINT-2734711.
STRINGi4932.YGR231C.

Structurei

3D structure databases

ProteinModelPortaliP50085.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili212 – 253421 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the prohibitin family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0330.
GeneTreeiENSGT00550000075076.
HOGENOMiHOG000205692.
InParanoidiP50085.
KOiK17081.
OMAiNIQDSGF.
OrthoDBiEOG7BS4MS.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRSPGEFQR YAKAFQKQLS KVQQTGGRGQ VPSPRGAFAG LGGLLLLGGG
60 70 80 90 100
ALFINNALFN VDGGHRAIVY SRIHGVSSRI FNEGTHFIFP WLDTPIIYDV
110 120 130 140 150
RAKPRNVASL TGTKDLQMVN ITCRVLSRPD VVQLPTIYRT LGQDYDERVL
160 170 180 190 200
PSIVNEVLKA VVAQFNASQL ITQREKVSRL IRENLVRRAS KFNILLDDVS
210 220 230 240 250
ITYMTFSPEF TNAVEAKQIA QQDAQRAAFV VDKARQEKQG MVVRAQGEAK
260 270 280 290 300
SAELIGEAIK KSRDYVELKR LDTARDIAKI LASSPNRVIL DNEALLLNTV
310
VDARIDGRGK
Length:310
Mass (Da):34,407
Last modified:October 3, 2006 - v2
Checksum:i3526D9690F39B493
GO

Sequence cautioni

The sequence CAA61181.1 differs from that shown. Reason: Frameshift at position 310. Curated
The sequence CAA97259.1 differs from that shown. Reason: Frameshift at position 310. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61181.1. Frameshift.
Z73016 Genomic DNA. Translation: CAA97259.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08322.1.
PIRiS57696.
RefSeqiNP_011747.2. NM_001181360.1.

Genome annotation databases

EnsemblFungiiYGR231C; YGR231C; YGR231C.
GeneIDi853146.
KEGGisce:YGR231C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61181.1. Frameshift.
Z73016 Genomic DNA. Translation: CAA97259.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08322.1.
PIRiS57696.
RefSeqiNP_011747.2. NM_001181360.1.

3D structure databases

ProteinModelPortaliP50085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33483. 165 interactions.
IntActiP50085. 42 interactions.
MINTiMINT-2734711.
STRINGi4932.YGR231C.

Proteomic databases

MaxQBiP50085.
PaxDbiP50085.
PeptideAtlasiP50085.
PRIDEiP50085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR231C; YGR231C; YGR231C.
GeneIDi853146.
KEGGisce:YGR231C.

Organism-specific databases

CYGDiYGR231c.
EuPathDBiFungiDB:YGR231C.
SGDiS000003463. PHB2.

Phylogenomic databases

eggNOGiCOG0330.
GeneTreeiENSGT00550000075076.
HOGENOMiHOG000205692.
InParanoidiP50085.
KOiK17081.
OMAiNIQDSGF.
OrthoDBiEOG7BS4MS.

Enzyme and pathway databases

BioCyciYEAST:G3O-30909-MONOMER.

Miscellaneous databases

NextBioi973222.
PROiP50085.

Gene expression databases

GenevestigatoriP50085.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
    van der Aart Q.J.M., Kleine K., Steensma H.Y.
    Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins."
    Nijtmans L.G.J., de Jong L., Artal-Sanz M., Coates P.J., Berden J.A., Back J.W., Muijsers A.O., van der Spek H., Grivell L.A.
    EMBO J. 19:2444-2451(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "A structure for the yeast prohibitin complex: structure prediction and evidence from chemical crosslinking and mass spectrometry."
    Back J.W., Artal-Sanz M., De Jong L., De Koning L.J., Nijtmans L.G.J., De Koster C.G., Grivell L.A., Van Der Spek H., Muijsers A.O.
    Protein Sci. 11:2471-2478(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  10. "Formation of membrane-bound ring complexes by prohibitins in mitochondria."
    Tatsuta T., Model K., Langer T.
    Mol. Biol. Cell 16:248-259(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SINGLE PARTICLE ELECTRON MICROSCOPY, COILED-COIL DOMAIN.

Entry informationi

Entry nameiPHB2_YEAST
AccessioniPrimary (citable) accession number: P50085
Secondary accession number(s): D6VV11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: May 27, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2850 molecules/cell in log phase SD medium.1 Publication
Mitochondrial targeting of PHB2 is ensured by a bipartite non-cleavable presequence at the N-terminus.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.