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P50085

- PHB2_YEAST

UniProt

P50085 - PHB2_YEAST

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Protein

Prohibitin-2

Gene
PHB2, YGR231C, G8561
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. mitochondrion inheritance Source: SGD
  2. negative regulation of proteolysis Source: SGD
  3. protein folding Source: SGD
  4. replicative cell aging Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30909-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-2
Gene namesi
Name:PHB2
Ordered Locus Names:YGR231C
ORF Names:G8561
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR231c.
SGDiS000003463. PHB2.

Subcellular locationi

Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side 3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei38 – 5821Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: SGD
  3. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Prohibitin-2PRO_0000213888Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP50085.
PaxDbiP50085.
PeptideAtlasiP50085.
PRIDEiP50085.

Expressioni

Gene expression databases

GenevestigatoriP50085.

Interactioni

Subunit structurei

The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHB1P409613EBI-23530,EBI-13360

Protein-protein interaction databases

BioGridi33483. 164 interactions.
IntActiP50085. 41 interactions.
MINTiMINT-2734711.
STRINGi4932.YGR231C.

Structurei

3D structure databases

ProteinModelPortaliP50085.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili212 – 253421 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the prohibitin family.

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0330.
GeneTreeiENSGT00550000075076.
HOGENOMiHOG000205692.
KOiK17081.
OMAiTYMTFSP.
OrthoDBiEOG7BS4MS.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50085-1 [UniParc]FASTAAdd to Basket

« Hide

MNRSPGEFQR YAKAFQKQLS KVQQTGGRGQ VPSPRGAFAG LGGLLLLGGG    50
ALFINNALFN VDGGHRAIVY SRIHGVSSRI FNEGTHFIFP WLDTPIIYDV 100
RAKPRNVASL TGTKDLQMVN ITCRVLSRPD VVQLPTIYRT LGQDYDERVL 150
PSIVNEVLKA VVAQFNASQL ITQREKVSRL IRENLVRRAS KFNILLDDVS 200
ITYMTFSPEF TNAVEAKQIA QQDAQRAAFV VDKARQEKQG MVVRAQGEAK 250
SAELIGEAIK KSRDYVELKR LDTARDIAKI LASSPNRVIL DNEALLLNTV 300
VDARIDGRGK 310
Length:310
Mass (Da):34,407
Last modified:October 3, 2006 - v2
Checksum:i3526D9690F39B493
GO

Sequence cautioni

The sequence CAA61181.1 differs from that shown. Reason: Frameshift at position 310.
The sequence CAA97259.1 differs from that shown. Reason: Frameshift at position 310.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87941 Genomic DNA. Translation: CAA61181.1. Frameshift.
Z73016 Genomic DNA. Translation: CAA97259.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08322.1.
PIRiS57696.
RefSeqiNP_011747.2. NM_001181360.1.

Genome annotation databases

EnsemblFungiiYGR231C; YGR231C; YGR231C.
GeneIDi853146.
KEGGisce:YGR231C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87941 Genomic DNA. Translation: CAA61181.1 . Frameshift.
Z73016 Genomic DNA. Translation: CAA97259.1 . Frameshift.
BK006941 Genomic DNA. Translation: DAA08322.1 .
PIRi S57696.
RefSeqi NP_011747.2. NM_001181360.1.

3D structure databases

ProteinModelPortali P50085.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33483. 164 interactions.
IntActi P50085. 41 interactions.
MINTi MINT-2734711.
STRINGi 4932.YGR231C.

Proteomic databases

MaxQBi P50085.
PaxDbi P50085.
PeptideAtlasi P50085.
PRIDEi P50085.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR231C ; YGR231C ; YGR231C .
GeneIDi 853146.
KEGGi sce:YGR231C.

Organism-specific databases

CYGDi YGR231c.
SGDi S000003463. PHB2.

Phylogenomic databases

eggNOGi COG0330.
GeneTreei ENSGT00550000075076.
HOGENOMi HOG000205692.
KOi K17081.
OMAi TYMTFSP.
OrthoDBi EOG7BS4MS.

Enzyme and pathway databases

BioCyci YEAST:G3O-30909-MONOMER.

Miscellaneous databases

NextBioi 973222.
PROi P50085.

Gene expression databases

Genevestigatori P50085.

Family and domain databases

InterProi IPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view ]
PANTHERi PTHR23222. PTHR23222. 1 hit.
Pfami PF01145. Band_7. 1 hit.
[Graphical view ]
PRINTSi PR00679. PROHIBITIN.
SMARTi SM00244. PHB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
    van der Aart Q.J.M., Kleine K., Steensma H.Y.
    Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins."
    Nijtmans L.G.J., de Jong L., Artal-Sanz M., Coates P.J., Berden J.A., Back J.W., Muijsers A.O., van der Spek H., Grivell L.A.
    EMBO J. 19:2444-2451(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "A structure for the yeast prohibitin complex: structure prediction and evidence from chemical crosslinking and mass spectrometry."
    Back J.W., Artal-Sanz M., De Jong L., De Koning L.J., Nijtmans L.G.J., De Koster C.G., Grivell L.A., Van Der Spek H., Muijsers A.O.
    Protein Sci. 11:2471-2478(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  10. "Formation of membrane-bound ring complexes by prohibitins in mitochondria."
    Tatsuta T., Model K., Langer T.
    Mol. Biol. Cell 16:248-259(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SINGLE PARTICLE ELECTRON MICROSCOPY, COILED-COIL DOMAIN.

Entry informationi

Entry nameiPHB2_YEAST
AccessioniPrimary (citable) accession number: P50085
Secondary accession number(s): D6VV11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2850 molecules/cell in log phase SD medium.
Mitochondrial targeting of PHB2 is ensured by a bipartite non-cleavable presequence at the N-terminus.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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