ID   AMA1_YEAST              Reviewed;         593 AA.
AC   P50082; D6VV07; Q96VQ3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 2.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=Meiosis-specific APC/C activator protein AMA1;
DE   AltName: Full=Activator of meiotic APC/C protein 1;
DE   AltName: Full=Sporulation-specific protein 70;
GN   Name=AMA1; Synonyms=SPO70; OrderedLocusNames=YGR225W; ORFNames=G8541;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INTERACTION WITH CDC16.
RX   PubMed=11114178; DOI=10.1073/pnas.250351297;
RA   Cooper K.F., Mallory M.J., Egeland D.B., Jarnik M., Strich R.;
RT   "Ama1p is a meiosis-specific regulator of the anaphase promoting
RT   complex/cyclosome in yeast.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:14548-14553(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8701610;
RX   DOI=10.1002/(sici)1097-0061(19960330)12:4<385::aid-yea910>3.0.co;2-g;
RA   van der Aart Q.J.M., Kleine K., Steensma H.Y.;
RT   "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1
RT   region from the right arm of Saccharomyces cerevisiae chromosome VII.";
RL   Yeast 12:385-390(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=9784122; DOI=10.1126/science.282.5389.699;
RA   Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P.O.,
RA   Herskowitz I.;
RT   "The transcriptional program of sporulation in budding yeast.";
RL   Science 282:699-705(1998).
RN   [6]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=10734188; DOI=10.1093/nar/28.8.1700;
RA   Davis C.A., Grate L., Spingola M., Ares M. Jr.;
RT   "Test of intron predictions reveals novel splice sites, alternatively
RT   spliced mRNAs and new introns in meiotically regulated genes of yeast.";
RL   Nucleic Acids Res. 28:1700-1706(2000).
CC   -!- FUNCTION: Activator protein that regulates the ubiquitin ligase
CC       activity and substrate specificity of the anaphase promoting
CC       complex/cyclosome (APC/C). Required for the ubiquitination and
CC       subsequent degradation of the B-type cyclin CLB1 by the APC/C complex
CC       during meiosis. Required for meiosis I, late meiotic gene expression
CC       and spore wall assembly. {ECO:0000269|PubMed:11114178,
CC       ECO:0000269|PubMed:9784122}.
CC   -!- SUBUNIT: Interacts with CDC16. {ECO:0000269|PubMed:11114178}.
CC   -!- DEVELOPMENTAL STAGE: Expressed and spliced only during meiosis
CC       dependent on the splicing factor MER1. {ECO:0000269|PubMed:10734188,
CC       ECO:0000269|PubMed:9784122}.
CC   -!- DOMAIN: The C-box is required for the association with the APC/C
CC       complex. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA61174.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAA61174.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=CAA97253.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY033394; AAK61800.1; -; Genomic_DNA.
DR   EMBL; X87941; CAA61174.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; Z73010; CAA97253.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BK006941; DAA08318.1; -; Genomic_DNA.
DR   PIR; S57689; S57689.
DR   RefSeq; NP_011741.5; NM_001181354.3.
DR   AlphaFoldDB; P50082; -.
DR   SMR; P50082; -.
DR   BioGRID; 33478; 80.
DR   ComplexPortal; CPX-762; Anaphase-Promoting complex AMA1 variant.
DR   DIP; DIP-7218N; -.
DR   ELM; P50082; -.
DR   IntAct; P50082; 3.
DR   MINT; P50082; -.
DR   STRING; 4932.YGR225W; -.
DR   PaxDb; 4932-YGR225W; -.
DR   PeptideAtlas; P50082; -.
DR   EnsemblFungi; YGR225W_mRNA; YGR225W; YGR225W.
DR   GeneID; 853140; -.
DR   KEGG; sce:YGR225W; -.
DR   AGR; SGD:S000003457; -.
DR   SGD; S000003457; AMA1.
DR   VEuPathDB; FungiDB:YGR225W; -.
DR   eggNOG; KOG0305; Eukaryota.
DR   GeneTree; ENSGT00950000183104; -.
DR   HOGENOM; CLU_014831_3_3_1; -.
DR   InParanoid; P50082; -.
DR   OMA; SICIATN; -.
DR   OrthoDB; 20041at2759; -.
DR   BioCyc; YEAST:G3O-30906-MONOMER; -.
DR   BioGRID-ORCS; 853140; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P50082; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P50082; Protein.
DR   GO; GO:0005680; C:anaphase-promoting complex; IPI:SGD.
DR   GO; GO:0010997; F:anaphase-promoting complex binding; IBA:GO_Central.
DR   GO; GO:1990757; F:ubiquitin ligase activator activity; IDA:SGD.
DR   GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR   GO; GO:0030437; P:ascospore formation; IMP:SGD.
DR   GO; GO:0030476; P:ascospore wall assembly; IMP:SGD.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007127; P:meiosis I; IMP:SGD.
DR   GO; GO:0045132; P:meiotic chromosome segregation; IMP:SGD.
DR   GO; GO:0044778; P:meiotic DNA integrity checkpoint signaling; IMP:SGD.
DR   GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IMP:SGD.
DR   GO; GO:1903024; P:positive regulation of ascospore-type prospore membrane formation; IGI:SGD.
DR   GO; GO:0045732; P:positive regulation of protein catabolic process; IMP:SGD.
DR   GO; GO:0031398; P:positive regulation of protein ubiquitination; IDA:SGD.
DR   GO; GO:0016567; P:protein ubiquitination; NAS:ComplexPortal.
DR   GO; GO:0051445; P:regulation of meiotic cell cycle; NAS:ComplexPortal.
DR   GO; GO:0007130; P:synaptonemal complex assembly; IMP:SGD.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR033010; Cdc20/Fizzy.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR19918; CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED; 1.
DR   PANTHER; PTHR19918:SF5; MEIOSIS-SPECIFIC APC_C ACTIVATOR PROTEIN AMA1; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 5.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Meiosis; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..593
FT                   /note="Meiosis-specific APC/C activator protein AMA1"
FT                   /id="PRO_0000050842"
FT   REPEAT          226..264
FT                   /note="WD 1"
FT   REPEAT          271..310
FT                   /note="WD 2"
FT   REPEAT          323..364
FT                   /note="WD 3"
FT   REPEAT          388..427
FT                   /note="WD 4"
FT   REPEAT          432..474
FT                   /note="WD 5"
FT   REPEAT          525..564
FT                   /note="WD 6"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          94..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           29..35
FT                   /note="C-box"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        8..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        94..115
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   593 AA;  67027 MW;  35685463E1A677CA CRC64;
     MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF DISYSELCEK
     SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS ESQVTRSGSA RASRNDYSKL
     TKEQKDHRKN IAHSLGFQLP DRVFTFETTS AEILEKNKAI KNCFGPGSCA EIRSTFDFST
     LSPDVARYYI ANSNARSASP QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT
     NNVLVGLGCS VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY
     DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN VSLFEIKSLH
     FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE HANLLAVGGN DNSCSLWDIS
     DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ
     VTSLIWSLRH KQIVATFGFG DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM
     AICVATNDET IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR
//