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P50082

- AMA1_YEAST

UniProt

P50082 - AMA1_YEAST

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Protein

Meiosis-specific APC/C activator protein AMA1

Gene
AMA1, SPO70, YGR225W, G8541
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly.2 Publications

GO - Molecular functioni

  1. enzyme activator activity Source: SGD

GO - Biological processi

  1. ascospore formation Source: SGD
  2. ascospore wall assembly Source: SGD
  3. meiosis I Source: SGD
  4. meiotic chromosome segregation Source: SGD
  5. meiotic DNA integrity checkpoint Source: SGD
  6. positive regulation of catalytic activity Source: GOC
  7. positive regulation of protein catabolic process Source: SGD
  8. proteolysis Source: SGD
  9. synaptonemal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30906-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific APC/C activator protein AMA1
Alternative name(s):
Activator of meiotic APC/C protein 1
Sporulation-specific protein 70
Gene namesi
Name:AMA1
Synonyms:SPO70
Ordered Locus Names:YGR225W
ORF Names:G8541
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR225w.
SGDiS000003457. AMA1.

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Meiosis-specific APC/C activator protein AMA1PRO_0000050842Add
BLAST

Proteomic databases

PeptideAtlasiP50082.

Expressioni

Developmental stagei

Expressed and spliced only during meiosis dependent on the splicing factor MER1.2 Publications

Gene expression databases

GenevestigatoriP50082.

Interactioni

Subunit structurei

Interacts with CDC16.1 Publication

Protein-protein interaction databases

BioGridi33478. 20 interactions.
DIPiDIP-8992N.
IntActiP50082. 3 interactions.
MINTiMINT-7266666.
STRINGi4932.YGR225W.

Structurei

3D structure databases

ProteinModelPortaliP50082.
SMRiP50082. Positions 215-554.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati226 – 26439WD 1Add
BLAST
Repeati271 – 31040WD 2Add
BLAST
Repeati323 – 36442WD 3Add
BLAST
Repeati388 – 42740WD 4Add
BLAST
Repeati432 – 47443WD 5Add
BLAST
Repeati525 – 56440WD 6Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi29 – 357C-box By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi94 – 1007Poly-Ser

Domaini

The C-box is required for the association with the APC/C complex By similarity.

Sequence similaritiesi

Contains 6 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00750000118716.
HOGENOMiHOG000033911.
KOiK12782.
OMAiLRNDFYS.
OrthoDBiEOG7CZKFT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50082-1 [UniParc]FASTAAdd to Basket

« Hide

MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF    50
DISYSELCEK SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS 100
ESQVTRSGSA RASRNDYSKL TKEQKDHRKN IAHSLGFQLP DRVFTFETTS 150
AEILEKNKAI KNCFGPGSCA EIRSTFDFST LSPDVARYYI ANSNARSASP 200
QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT NNVLVGLGCS 250
VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY 300
DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN 350
VSLFEIKSLH FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE 400
HANLLAVGGN DNSCSLWDIS DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL 450
ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ VTSLIWSLRH KQIVATFGFG 500
DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM AICVATNDET 550
IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR 593
Length:593
Mass (Da):67,027
Last modified:January 4, 2005 - v2
Checksum:i35685463E1A677CA
GO

Sequence cautioni

The sequence CAA61174.1 differs from that shown. Reason: Frameshift at position 554.
The sequence CAA61174.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA97253.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY033394 Genomic DNA. Translation: AAK61800.1.
X87941 Genomic DNA. Translation: CAA61174.1. Sequence problems.
Z73010 Genomic DNA. Translation: CAA97253.1. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08318.1.
PIRiS57689.
RefSeqiNP_011741.5. NM_001181354.3.

Genome annotation databases

EnsemblFungiiYGR225W; YGR225W; YGR225W.
GeneIDi853140.
KEGGisce:YGR225W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY033394 Genomic DNA. Translation: AAK61800.1 .
X87941 Genomic DNA. Translation: CAA61174.1 . Sequence problems.
Z73010 Genomic DNA. Translation: CAA97253.1 . Sequence problems.
BK006941 Genomic DNA. Translation: DAA08318.1 .
PIRi S57689.
RefSeqi NP_011741.5. NM_001181354.3.

3D structure databases

ProteinModelPortali P50082.
SMRi P50082. Positions 215-554.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33478. 20 interactions.
DIPi DIP-8992N.
IntActi P50082. 3 interactions.
MINTi MINT-7266666.
STRINGi 4932.YGR225W.

Proteomic databases

PeptideAtlasi P50082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR225W ; YGR225W ; YGR225W .
GeneIDi 853140.
KEGGi sce:YGR225W.

Organism-specific databases

CYGDi YGR225w.
SGDi S000003457. AMA1.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00750000118716.
HOGENOMi HOG000033911.
KOi K12782.
OMAi LRNDFYS.
OrthoDBi EOG7CZKFT.

Enzyme and pathway databases

BioCyci YEAST:G3O-30906-MONOMER.

Miscellaneous databases

NextBioi 973210.

Gene expression databases

Genevestigatori P50082.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 2 hits.
[Graphical view ]
SMARTi SM00320. WD40. 5 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast."
    Cooper K.F., Mallory M.J., Egeland D.B., Jarnik M., Strich R.
    Proc. Natl. Acad. Sci. U.S.A. 97:14548-14553(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH CDC16.
  2. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
    van der Aart Q.J.M., Kleine K., Steensma H.Y.
    Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The transcriptional program of sporulation in budding yeast."
    Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P.O., Herskowitz I.
    Science 282:699-705(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  6. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
    Davis C.A., Grate L., Spingola M., Ares M. Jr.
    Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiAMA1_YEAST
AccessioniPrimary (citable) accession number: P50082
Secondary accession number(s): D6VV07, Q96VQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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