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P50082 (AMA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Meiosis-specific APC/C activator protein AMA1
Alternative name(s):
Activator of meiotic APC/C protein 1
Sporulation-specific protein 70
Gene names
Name:AMA1
Synonyms:SPO70
Ordered Locus Names:YGR225W
ORF Names:G8541
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly. Ref.1 Ref.5

Subunit structure

Interacts with CDC16. Ref.1

Developmental stage

Expressed and spliced only during meiosis dependent on the splicing factor MER1. Ref.5 Ref.6

Domain

The C-box is required for the association with the APC/C complex By similarity.

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.

Contains 6 WD repeats.

Sequence caution

The sequence CAA61174.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA61174.1 differs from that shown. Reason: Frameshift at position 554.

The sequence CAA97253.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 593593Meiosis-specific APC/C activator protein AMA1
PRO_0000050842

Regions

Repeat226 – 26439WD 1
Repeat271 – 31040WD 2
Repeat323 – 36442WD 3
Repeat388 – 42740WD 4
Repeat432 – 47443WD 5
Repeat525 – 56440WD 6
Motif29 – 357C-box By similarity
Compositional bias94 – 1007Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
P50082 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 35685463E1A677CA

FASTA59367,027
        10         20         30         40         50         60 
MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF DISYSELCEK 

        70         80         90        100        110        120 
SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS ESQVTRSGSA RASRNDYSKL 

       130        140        150        160        170        180 
TKEQKDHRKN IAHSLGFQLP DRVFTFETTS AEILEKNKAI KNCFGPGSCA EIRSTFDFST 

       190        200        210        220        230        240 
LSPDVARYYI ANSNARSASP QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT 

       250        260        270        280        290        300 
NNVLVGLGCS VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY 

       310        320        330        340        350        360 
DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN VSLFEIKSLH 

       370        380        390        400        410        420 
FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE HANLLAVGGN DNSCSLWDIS 

       430        440        450        460        470        480 
DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ 

       490        500        510        520        530        540 
VTSLIWSLRH KQIVATFGFG DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM 

       550        560        570        580        590 
AICVATNDET IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR 

« Hide

References

« Hide 'large scale' references
[1]"Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast."
Cooper K.F., Mallory M.J., Egeland D.B., Jarnik M., Strich R.
Proc. Natl. Acad. Sci. U.S.A. 97:14548-14553(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH CDC16.
[2]"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
van der Aart Q.J.M., Kleine K., Steensma H.Y.
Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The transcriptional program of sporulation in budding yeast."
Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P.O., Herskowitz I.
Science 282:699-705(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
Davis C.A., Grate L., Spingola M., Ares M. Jr.
Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY033394 Genomic DNA. Translation: AAK61800.1.
X87941 Genomic DNA. Translation: CAA61174.1. Sequence problems.
Z73010 Genomic DNA. Translation: CAA97253.1. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08318.1.
PIRS57689.
RefSeqNP_011741.5. NM_001181354.3.

3D structure databases

ProteinModelPortalP50082.
SMRP50082. Positions 215-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33478. 20 interactions.
DIPDIP-8992N.
IntActP50082. 3 interactions.
MINTMINT-7266666.
STRING4932.YGR225W.

Proteomic databases

PeptideAtlasP50082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR225W; YGR225W; YGR225W.
GeneID853140.
KEGGsce:YGR225W.

Organism-specific databases

CYGDYGR225w.
SGDS000003457. AMA1.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00750000118716.
HOGENOMHOG000033911.
KOK12782.
OMALRNDFYS.
OrthoDBEOG7CZKFT.

Enzyme and pathway databases

BioCycYEAST:G3O-30906-MONOMER.

Gene expression databases

GenevestigatorP50082.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973210.

Entry information

Entry nameAMA1_YEAST
AccessionPrimary (citable) accession number: P50082
Secondary accession number(s): D6VV07, Q96VQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families