P50082 (AMA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Meiosis-specific APC/C activator protein AMA1

Alternative name(s):
Activator of meiotic APC/C protein 1
Sporulation-specific protein 70

Gene names

Name:	AMA1
Synonyms:	SPO70
Ordered Locus Names:	YGR225W
ORF Names:	G8541

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly. Ref.1 Ref.5
Subunit structure	Interacts with CDC16. Ref.1
Developmental stage	Expressed and spliced only during meiosis dependent on the splicing factor MER1. Ref.5 Ref.6
Domain	The C-box is required for the association with the APC/C complex By similarity.
Sequence similarities	Belongs to the WD repeat CDC20/Fizzy family. Contains 6 WD repeats.
Sequence caution	The sequence CAA61174.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA61174.1 differs from that shown. Reason: Frameshift at position 554. The sequence CAA97253.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ascospore wall assembly Inferred from mutant phenotype PubMed 15590821. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis I Inferred from mutant phenotype Ref.1. Source: SGD positive regulation of protein catabolic process Inferred from mutant phenotype Ref.1PubMed 21118994. Source: SGD
Cellular_component	anaphase-promoting complex Inferred from physical interaction Ref.1. Source: SGD
Molecular_function	enzyme activator activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Meiosis-specific APC/C activator protein AMA1		PRO_0000050842
Regions
	Repeat	226 – 264	39	WD 1
	Repeat	271 – 310	40	WD 2
	Repeat	323 – 364	42	WD 3
	Repeat	388 – 427	40	WD 4
	Repeat	432 – 474	43	WD 5
	Repeat	525 – 564	40	WD 6
	Motif	29 – 35	7	C-box By similarity
	Compositional bias	94 – 100	7	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	P50082 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 35685463E1A677CA	FASTA	593	67,027
	10 20 30 40 50 60 MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF DISYSELCEK 70 80 90 100 110 120 SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS ESQVTRSGSA RASRNDYSKL 130 140 150 160 170 180 TKEQKDHRKN IAHSLGFQLP DRVFTFETTS AEILEKNKAI KNCFGPGSCA EIRSTFDFST 190 200 210 220 230 240 LSPDVARYYI ANSNARSASP QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT 250 260 270 280 290 300 NNVLVGLGCS VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY 310 320 330 340 350 360 DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN VSLFEIKSLH 370 380 390 400 410 420 FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE HANLLAVGGN DNSCSLWDIS 430 440 450 460 470 480 DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ 490 500 510 520 530 540 VTSLIWSLRH KQIVATFGFG DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM 550 560 570 580 590 AICVATNDET IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast." Cooper K.F., Mallory M.J., Egeland D.B., Jarnik M., Strich R. Proc. Natl. Acad. Sci. U.S.A. 97:14548-14553(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH CDC16.
[2]	"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The transcriptional program of sporulation in budding yeast." Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P.O., Herskowitz I. Science 282:699-705(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]	"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast." Davis C.A., Grate L., Spingola M., Ares M. Jr. Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY033394 Genomic DNA. Translation: AAK61800.1. X87941 Genomic DNA. Translation: CAA61174.1. Sequence problems. Z73010 Genomic DNA. Translation: CAA97253.1. Sequence problems. BK006941 Genomic DNA. Translation: DAA08318.1.
PIR	S57689.
RefSeq	NP_011741.5. NM_001181354.3.
3D structure databases
ProteinModelPortal	P50082.
SMR	P50082. Positions 215-554.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8992N.
IntAct	P50082. 2 interactions.
MINT	MINT-7266666.
STRING	4932.YGR225W.
Proteomic databases
PeptideAtlas	P50082.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR225W; YGR225W; YGR225W.
GeneID	853140.
KEGG	sce:YGR225W.
Organism-specific databases
CYGD	YGR225w.
SGD	S000003457. AMA1.
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000033911.
KO	K12782.
OMA	LRNDFYS.
OrthoDB	EOG4936SQ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30906-MONOMER.
Gene expression databases
Genevestigator	P50082.
GermOnline	YGR225W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 2 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 1 hit. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973210.

Entry information

Entry name

AMA1_YEAST

Accession

Primary (citable) accession number: P50082
Secondary accession number(s): D6VV07, Q96VQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 4, 2005
Last modified:	May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program