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P50082

- AMA1_YEAST

UniProt

P50082 - AMA1_YEAST

Protein

Meiosis-specific APC/C activator protein AMA1

Gene

AMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly.2 Publications

    GO - Molecular functioni

    1. enzyme activator activity Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. ascospore wall assembly Source: SGD
    3. meiosis I Source: SGD
    4. meiotic chromosome segregation Source: SGD
    5. meiotic DNA integrity checkpoint Source: SGD
    6. positive regulation of catalytic activity Source: GOC
    7. positive regulation of protein catabolic process Source: SGD
    8. proteolysis Source: SGD
    9. synaptonemal complex assembly Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30906-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Meiosis-specific APC/C activator protein AMA1
    Alternative name(s):
    Activator of meiotic APC/C protein 1
    Sporulation-specific protein 70
    Gene namesi
    Name:AMA1
    Synonyms:SPO70
    Ordered Locus Names:YGR225W
    ORF Names:G8541
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR225w.
    SGDiS000003457. AMA1.

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 593593Meiosis-specific APC/C activator protein AMA1PRO_0000050842Add
    BLAST

    Proteomic databases

    PeptideAtlasiP50082.

    Expressioni

    Developmental stagei

    Expressed and spliced only during meiosis dependent on the splicing factor MER1.2 Publications

    Gene expression databases

    GenevestigatoriP50082.

    Interactioni

    Subunit structurei

    Interacts with CDC16.1 Publication

    Protein-protein interaction databases

    BioGridi33478. 20 interactions.
    DIPiDIP-8992N.
    IntActiP50082. 3 interactions.
    MINTiMINT-7266666.
    STRINGi4932.YGR225W.

    Structurei

    3D structure databases

    ProteinModelPortaliP50082.
    SMRiP50082. Positions 215-554.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati226 – 26439WD 1Add
    BLAST
    Repeati271 – 31040WD 2Add
    BLAST
    Repeati323 – 36442WD 3Add
    BLAST
    Repeati388 – 42740WD 4Add
    BLAST
    Repeati432 – 47443WD 5Add
    BLAST
    Repeati525 – 56440WD 6Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi29 – 357C-boxBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi94 – 1007Poly-Ser

    Domaini

    The C-box is required for the association with the APC/C complex.By similarity

    Sequence similaritiesi

    Belongs to the WD repeat CDC20/Fizzy family.Curated
    Contains 6 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00750000118716.
    HOGENOMiHOG000033911.
    KOiK12782.
    OMAiLRNDFYS.
    OrthoDBiEOG7CZKFT.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50082-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF    50
    DISYSELCEK SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS 100
    ESQVTRSGSA RASRNDYSKL TKEQKDHRKN IAHSLGFQLP DRVFTFETTS 150
    AEILEKNKAI KNCFGPGSCA EIRSTFDFST LSPDVARYYI ANSNARSASP 200
    QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT NNVLVGLGCS 250
    VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY 300
    DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN 350
    VSLFEIKSLH FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE 400
    HANLLAVGGN DNSCSLWDIS DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL 450
    ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ VTSLIWSLRH KQIVATFGFG 500
    DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM AICVATNDET 550
    IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR 593
    Length:593
    Mass (Da):67,027
    Last modified:January 4, 2005 - v2
    Checksum:i35685463E1A677CA
    GO

    Sequence cautioni

    The sequence CAA61174.1 differs from that shown. Reason: Frameshift at position 554.
    The sequence CAA61174.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA97253.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY033394 Genomic DNA. Translation: AAK61800.1.
    X87941 Genomic DNA. Translation: CAA61174.1. Sequence problems.
    Z73010 Genomic DNA. Translation: CAA97253.1. Sequence problems.
    BK006941 Genomic DNA. Translation: DAA08318.1.
    PIRiS57689.
    RefSeqiNP_011741.5. NM_001181354.3.

    Genome annotation databases

    EnsemblFungiiYGR225W; YGR225W; YGR225W.
    GeneIDi853140.
    KEGGisce:YGR225W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY033394 Genomic DNA. Translation: AAK61800.1 .
    X87941 Genomic DNA. Translation: CAA61174.1 . Sequence problems.
    Z73010 Genomic DNA. Translation: CAA97253.1 . Sequence problems.
    BK006941 Genomic DNA. Translation: DAA08318.1 .
    PIRi S57689.
    RefSeqi NP_011741.5. NM_001181354.3.

    3D structure databases

    ProteinModelPortali P50082.
    SMRi P50082. Positions 215-554.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33478. 20 interactions.
    DIPi DIP-8992N.
    IntActi P50082. 3 interactions.
    MINTi MINT-7266666.
    STRINGi 4932.YGR225W.

    Proteomic databases

    PeptideAtlasi P50082.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR225W ; YGR225W ; YGR225W .
    GeneIDi 853140.
    KEGGi sce:YGR225W.

    Organism-specific databases

    CYGDi YGR225w.
    SGDi S000003457. AMA1.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00750000118716.
    HOGENOMi HOG000033911.
    KOi K12782.
    OMAi LRNDFYS.
    OrthoDBi EOG7CZKFT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30906-MONOMER.

    Miscellaneous databases

    NextBioi 973210.

    Gene expression databases

    Genevestigatori P50082.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast."
      Cooper K.F., Mallory M.J., Egeland D.B., Jarnik M., Strich R.
      Proc. Natl. Acad. Sci. U.S.A. 97:14548-14553(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH CDC16.
    2. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
      van der Aart Q.J.M., Kleine K., Steensma H.Y.
      Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The transcriptional program of sporulation in budding yeast."
      Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P.O., Herskowitz I.
      Science 282:699-705(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    6. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
      Davis C.A., Grate L., Spingola M., Ares M. Jr.
      Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiAMA1_YEAST
    AccessioniPrimary (citable) accession number: P50082
    Secondary accession number(s): D6VV07, Q96VQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3