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Protein

Meiosis-specific APC/C activator protein AMA1

Gene

AMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly.2 Publications

GO - Molecular functioni

  • enzyme activator activity Source: SGD
  • ubiquitin-protein transferase activator activity Source: InterPro

GO - Biological processi

  • ascospore formation Source: SGD
  • ascospore wall assembly Source: SGD
  • cell division Source: UniProtKB-KW
  • meiosis I Source: SGD
  • meiotic chromosome segregation Source: SGD
  • meiotic DNA integrity checkpoint Source: SGD
  • positive regulation of protein catabolic process Source: SGD
  • positive regulation of ubiquitin protein ligase activity Source: InterPro
  • proteolysis Source: SGD
  • synaptonemal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30906-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific APC/C activator protein AMA1
Alternative name(s):
Activator of meiotic APC/C protein 1
Sporulation-specific protein 70
Gene namesi
Name:AMA1
Synonyms:SPO70
Ordered Locus Names:YGR225W
ORF Names:G8541
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR225W.
SGDiS000003457. AMA1.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508421 – 593Meiosis-specific APC/C activator protein AMA1Add BLAST593

Proteomic databases

PRIDEiP50082.

Expressioni

Developmental stagei

Expressed and spliced only during meiosis dependent on the splicing factor MER1.2 Publications

Interactioni

Subunit structurei

Interacts with CDC16.1 Publication

Protein-protein interaction databases

BioGridi33478. 19 interactors.
DIPiDIP-8992N.
IntActiP50082. 3 interactors.
MINTiMINT-7266666.

Structurei

3D structure databases

ProteinModelPortaliP50082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati226 – 264WD 1Add BLAST39
Repeati271 – 310WD 2Add BLAST40
Repeati323 – 364WD 3Add BLAST42
Repeati388 – 427WD 4Add BLAST40
Repeati432 – 474WD 5Add BLAST43
Repeati525 – 564WD 6Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 35C-boxBy similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi94 – 100Poly-Ser7

Domaini

The C-box is required for the association with the APC/C complex.By similarity

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000033911.
InParanoidiP50082.
KOiK12782.
OMAiRVLDAPC.
OrthoDBiEOG092C2LDD.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 4 hits.
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF
60 70 80 90 100
DISYSELCEK SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS
110 120 130 140 150
ESQVTRSGSA RASRNDYSKL TKEQKDHRKN IAHSLGFQLP DRVFTFETTS
160 170 180 190 200
AEILEKNKAI KNCFGPGSCA EIRSTFDFST LSPDVARYYI ANSNARSASP
210 220 230 240 250
QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT NNVLVGLGCS
260 270 280 290 300
VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY
310 320 330 340 350
DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN
360 370 380 390 400
VSLFEIKSLH FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE
410 420 430 440 450
HANLLAVGGN DNSCSLWDIS DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL
460 470 480 490 500
ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ VTSLIWSLRH KQIVATFGFG
510 520 530 540 550
DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM AICVATNDET
560 570 580 590
IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR
Length:593
Mass (Da):67,027
Last modified:January 4, 2005 - v2
Checksum:i35685463E1A677CA
GO

Sequence cautioni

The sequence CAA61174 differs from that shown. Reason: Frameshift at position 554.Curated
The sequence CAA61174 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA97253 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033394 Genomic DNA. Translation: AAK61800.1.
X87941 Genomic DNA. Translation: CAA61174.1. Sequence problems.
Z73010 Genomic DNA. Translation: CAA97253.1. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08318.1.
PIRiS57689.
RefSeqiNP_011741.5. NM_001181354.3.

Genome annotation databases

EnsemblFungiiYGR225W; YGR225W; YGR225W.
GeneIDi853140.
KEGGisce:YGR225W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033394 Genomic DNA. Translation: AAK61800.1.
X87941 Genomic DNA. Translation: CAA61174.1. Sequence problems.
Z73010 Genomic DNA. Translation: CAA97253.1. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08318.1.
PIRiS57689.
RefSeqiNP_011741.5. NM_001181354.3.

3D structure databases

ProteinModelPortaliP50082.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33478. 19 interactors.
DIPiDIP-8992N.
IntActiP50082. 3 interactors.
MINTiMINT-7266666.

Proteomic databases

PRIDEiP50082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR225W; YGR225W; YGR225W.
GeneIDi853140.
KEGGisce:YGR225W.

Organism-specific databases

EuPathDBiFungiDB:YGR225W.
SGDiS000003457. AMA1.

Phylogenomic databases

HOGENOMiHOG000033911.
InParanoidiP50082.
KOiK12782.
OMAiRVLDAPC.
OrthoDBiEOG092C2LDD.

Enzyme and pathway databases

BioCyciYEAST:G3O-30906-MONOMER.

Miscellaneous databases

PROiP50082.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 4 hits.
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMA1_YEAST
AccessioniPrimary (citable) accession number: P50082
Secondary accession number(s): D6VV07, Q96VQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 4, 2005
Last modified: November 30, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.