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Protein

Meiosis-specific APC/C activator protein AMA1

Gene

AMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). Required for the ubiquitination and subsequent degradation of the B-type cyclin CLB1 by the APC/C complex during meiosis. Required for meiosis I, late meiotic gene expression and spore wall assembly.2 Publications

GO - Molecular functioni

  • anaphase-promoting complex binding Source: InterPro
  • enzyme activator activity Source: SGD
  • ubiquitin-protein transferase activator activity Source: InterPro

GO - Biological processi

  • ascospore formation Source: SGD
  • ascospore wall assembly Source: SGD
  • cell division Source: UniProtKB-KW
  • meiosis I Source: SGD
  • meiotic chromosome segregation Source: SGD
  • meiotic DNA integrity checkpoint Source: SGD
  • positive regulation of protein catabolic process Source: SGD
  • positive regulation of ubiquitin protein ligase activity Source: InterPro
  • proteolysis Source: SGD
  • synaptonemal complex assembly Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30906-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific APC/C activator protein AMA1
Alternative name(s):
Activator of meiotic APC/C protein 1
Sporulation-specific protein 70
Gene namesi
Name:AMA1
Synonyms:SPO70
Ordered Locus Names:YGR225W
ORF Names:G8541
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR225W
SGDiS000003457 AMA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508421 – 593Meiosis-specific APC/C activator protein AMA1Add BLAST593

Proteomic databases

PaxDbiP50082
PRIDEiP50082

Expressioni

Developmental stagei

Expressed and spliced only during meiosis dependent on the splicing factor MER1.2 Publications

Interactioni

Subunit structurei

Interacts with CDC16.1 Publication

Protein-protein interaction databases

BioGridi33478, 61 interactors
ComplexPortaliCPX-762 Anaphase-Promoting Complex variant 4
DIPiDIP-7218N
ELMiP50082
IntActiP50082, 3 interactors
MINTiP50082
STRINGi4932.YGR225W

Structurei

3D structure databases

ProteinModelPortaliP50082
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati226 – 264WD 1Add BLAST39
Repeati271 – 310WD 2Add BLAST40
Repeati323 – 364WD 3Add BLAST42
Repeati388 – 427WD 4Add BLAST40
Repeati432 – 474WD 5Add BLAST43
Repeati525 – 564WD 6Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 35C-boxBy similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi94 – 100Poly-Ser7

Domaini

The C-box is required for the association with the APC/C complex.By similarity

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00870000137661
HOGENOMiHOG000033911
InParanoidiP50082
KOiK12782
OMAiRVLDAPC
OrthoDBiEOG092C2LDD

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR033010 Cdc20/Fizzy
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19918 PTHR19918, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P50082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF
60 70 80 90 100
DISYSELCEK SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS
110 120 130 140 150
ESQVTRSGSA RASRNDYSKL TKEQKDHRKN IAHSLGFQLP DRVFTFETTS
160 170 180 190 200
AEILEKNKAI KNCFGPGSCA EIRSTFDFST LSPDVARYYI ANSNARSASP
210 220 230 240 250
QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT NNVLVGLGCS
260 270 280 290 300
VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY
310 320 330 340 350
DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN
360 370 380 390 400
VSLFEIKSLH FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE
410 420 430 440 450
HANLLAVGGN DNSCSLWDIS DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL
460 470 480 490 500
ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ VTSLIWSLRH KQIVATFGFG
510 520 530 540 550
DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM AICVATNDET
560 570 580 590
IRFYELWNDK EEIINEIQES GIYGSNIIEY MEGIETTHNK RIR
Length:593
Mass (Da):67,027
Last modified:January 4, 2005 - v2
Checksum:i35685463E1A677CA
GO

Sequence cautioni

The sequence CAA61174 differs from that shown. Reason: Frameshift at position 554.Curated
The sequence CAA61174 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA97253 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033394 Genomic DNA Translation: AAK61800.1
X87941 Genomic DNA Translation: CAA61174.1 Sequence problems.
Z73010 Genomic DNA Translation: CAA97253.1 Sequence problems.
BK006941 Genomic DNA Translation: DAA08318.1
PIRiS57689
RefSeqiNP_011741.5, NM_001181354.3

Genome annotation databases

EnsemblFungiiYGR225W; YGR225W; YGR225W
GeneIDi853140
KEGGisce:YGR225W

Similar proteinsi

Entry informationi

Entry nameiAMA1_YEAST
AccessioniPrimary (citable) accession number: P50082
Secondary accession number(s): D6VV07, Q96VQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 4, 2005
Last modified: June 20, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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