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Protein

Azole resistance protein 1

Gene

AZR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter protein required for adaptation to high stress imposed by low-chain organic acids, in particular by acetic acid, and for resistance to azoles, especially to ketoconazole and fluconazole.1 Publication

GO - Molecular functioni

  • azole transporter activity Source: SGD

GO - Biological processi

  • azole transport Source: SGD
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30905-MONOMER.

Protein family/group databases

TCDBi2.A.1.3.53. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Azole resistance protein 1
Gene namesi
Name:AZR1
Ordered Locus Names:YGR224W
ORF Names:G8537
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR224W.
SGDiS000003456. AZR1.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7070ExtracellularSequence analysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence analysisAdd
BLAST
Topological domaini92 – 10211CytoplasmicSequence analysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Topological domaini124 – 13411ExtracellularSequence analysisAdd
BLAST
Transmembranei135 – 15521HelicalSequence analysisAdd
BLAST
Topological domaini156 – 1638CytoplasmicSequence analysis
Transmembranei164 – 18421HelicalSequence analysisAdd
BLAST
Topological domaini185 – 1895ExtracellularSequence analysis
Transmembranei190 – 21021HelicalSequence analysisAdd
BLAST
Topological domaini211 – 22111CytoplasmicSequence analysisAdd
BLAST
Transmembranei222 – 24221HelicalSequence analysisAdd
BLAST
Topological domaini243 – 29856ExtracellularSequence analysisAdd
BLAST
Transmembranei299 – 31921HelicalSequence analysisAdd
BLAST
Topological domaini320 – 32910CytoplasmicSequence analysis
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Topological domaini351 – 37525ExtracellularSequence analysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence analysisAdd
BLAST
Topological domaini397 – 41418CytoplasmicSequence analysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence analysisAdd
BLAST
Topological domaini436 – 4438ExtracellularSequence analysis
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST
Topological domaini465 – 4728CytoplasmicSequence analysis
Transmembranei473 – 49321HelicalSequence analysisAdd
BLAST
Topological domaini494 – 58188ExtracellularSequence analysisAdd
BLAST
Transmembranei582 – 60221HelicalSequence analysisAdd
BLAST
Topological domaini603 – 61311CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 613613Azole resistance protein 1PRO_0000173426Add
BLAST

Expressioni

Inductioni

Transcriptionally regulated by PDR8.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi33477. 15 interactions.
DIPiDIP-7543N.
IntActiP50080. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP50080.
SMRiP50080. Positions 94-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000247960.
InParanoidiP50080.
OMAiSAATNIM.
OrthoDBiEOG769ZTJ.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGEPKTYSM SDLSYYGEKA QQQNEKQQKQ YVVRRNSTQS TSKQNVSVVL
60 70 80 90 100
EDNASESNEL PKGFILYASL IALALSLFLA ALDIMIVSTI IEEVAKQFGS
110 120 130 140 150
YSEIGWLFTG YSLPNALLAL IWGRIATPIG FKETMLFAIV IFEIGSLISA
160 170 180 190 200
LANSMSMLIG GRVIAGVGGC GIQSLSFVIG STLVEESQRG ILIAVLSCSF
210 220 230 240 250
AIASVVGPFL GGVFTSSVTW RWCFYVNLPI GGLAFFLFLF FYNPGLSTFQ
260 270 280 290 300
ETMDNIRKFP SQFIEIVRNV AYHLLKIKGF SKLNGWRKPF MELIFMYDII
310 320 330 340 350
EFVFCSAGFT CILLAFTFGG NRYAWNSASI IILFIIGIVL VVLAGIYDFL
360 370 380 390 400
VFPKFNIVKA TPHYQPLMSW TNIKKPGIFT VNIALFLTCA GYISQFTYIV
410 420 430 440 450
QYFQLIYNDS AWRAAVHLVA CIISTVVTAI LCGAITDKTR QIKPIIVISS
460 470 480 490 500
IFGVVGAGIL TLLNNNANNS AHIGLLILPG VAFGGLAQSS MLASQIQLDK
510 520 530 540 550
KSPTFRSDFV SITTFNTFCK NLGQALGGVI SNTVFSAAAI KKLTKANIQL
560 570 580 590 600
PDGTTVDNLV IYRQTNFDGS HSKLGNIISE SLTDVFYMAL GFYALSLIFA
610
VFASNKKVTA SLR
Length:613
Mass (Da):67,169
Last modified:October 1, 1996 - v1
Checksum:iCF65C211E81D3FFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61172.1.
Z73009 Genomic DNA. Translation: CAA97252.1.
BK006941 Genomic DNA. Translation: DAA08317.1.
PIRiS57687.
RefSeqiNP_011740.3. NM_001181353.3.

Genome annotation databases

EnsemblFungiiYGR224W; YGR224W; YGR224W.
GeneIDi853139.
KEGGisce:YGR224W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87941 Genomic DNA. Translation: CAA61172.1.
Z73009 Genomic DNA. Translation: CAA97252.1.
BK006941 Genomic DNA. Translation: DAA08317.1.
PIRiS57687.
RefSeqiNP_011740.3. NM_001181353.3.

3D structure databases

ProteinModelPortaliP50080.
SMRiP50080. Positions 94-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33477. 15 interactions.
DIPiDIP-7543N.
IntActiP50080. 2 interactions.

Protein family/group databases

TCDBi2.A.1.3.53. the major facilitator superfamily (mfs).

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR224W; YGR224W; YGR224W.
GeneIDi853139.
KEGGisce:YGR224W.

Organism-specific databases

EuPathDBiFungiDB:YGR224W.
SGDiS000003456. AZR1.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000247960.
InParanoidiP50080.
OMAiSAATNIM.
OrthoDBiEOG769ZTJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30905-MONOMER.

Miscellaneous databases

PROiP50080.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII."
    van der Aart Q.J.M., Kleine K., Steensma H.Y.
    Yeast 12:385-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Expression of the AZR1 gene (ORF YGR224w), encoding a plasma membrane transporter of the major facilitator superfamily, is required for adaptation to acetic acid and resistance to azoles in Saccharomyces cerevisiae."
    Tenreiro S., Rosa P.C., Viegas C.A., Sa-Correia I.
    Yeast 16:1469-1481(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast."
    Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.
    J. Biol. Chem. 278:11427-11432(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiAZR1_YEAST
AccessioniPrimary (citable) accession number: P50080
Secondary accession number(s): D6VV06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.