Reviewed, UniProtKB/Swiss-Prot P50079 (HSV2_YEAST)

Names and origin

Protein names

Recommended name:
    SVP1-like protein 2

Gene names

Name:	HSV2
Ordered Locus Names:	YGR223C
ORF Names:	G8530

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in piecemeal microautophagy of the nucleus (micronucleophagy).
Subcellular location	Endosome membrane; Peripheral membrane protein. Prevacuolar compartment membrane; Peripheral membrane protein. Note: Recruitment to endosomes depends on binding to phosphatidylinositol 3-phosphate.
Domain	Contains a beta-propeller domain involved in specific binding of phosphatidylinositol 3,5-biphosphate.
Post-translational modification	N-glycosylated.
Sequence similarities	Belongs to the WD repeat SVP1 family. Contains 2 WD repeats.

Ontologies

Keywords
Cellular component	Endosome Membrane
Domain	Repeat WD repeat
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	piecemeal microautophagy of nucleus Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Ref.5 Inferred from direct assay. Source: SGD fungal-type vacuole Inferred from direct assay. Source: SGD peripheral to membrane of membrane fraction Ref.5 Inferred from direct assay. Source: SGD
Molecular function	phosphoinositide binding Ref.4 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAP2	P13517	1	EBI-23505,EBI-4013
ERG10	P41338	1	EBI-23505,EBI-19240
GLO2	Q05584	1	EBI-23505,EBI-7672
VAM7	P32912	1	EBI-23505,EBI-20232
VPS21	P36017	1	EBI-23505,EBI-29399

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	SVP1-like protein 2		PRO_0000051034
Regions
	Repeat	222 – 262	41	WD 1
	Repeat	267 – 306	40	WD 2
Amino acid modifications
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P50079-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A4287FAB80AA68DF	FASTA	448	51,235
	10 20 30 40 50 60 MDVRRPIREA VNNRRKPKFL SVSFNQDDSC FSVALENGFR IFNTDPLTSK LSKTFKESAT 70 80 90 100 110 120 NQSRGTGIGY TRMLYRTNYI ALVGGGKRPR HALNKLIIWD DLLQKETITL KFMSSIKDVF 130 140 150 160 170 180 LSRIHIVVVL ENTIEIFQFQ TNPQRICPIL DIPPNGSVDY VVCSSKHLQS QASQSQSKIL 190 200 210 220 230 240 EIIAFPSNKC VGQIQVADLS QIKYNSQNPK ESALLPTSII KAHKNPIKLV RLNRQGTMVA 250 260 270 280 290 300 TCSVQGTLIR IFSTHNGTLI KEFRRGVDKA DIYEMSFSPN GSKLAVLSNK QTLHIFQIFE 310 320 330 340 350 360 TTNTETNTPD HSRANGSSHP LKNYIPKGLW RPKYLDSVWS ICNAHLKNPI FDAHRNDNSG 370 380 390 400 410 420 DVTHDNEFYK DRCRIGWCQD SNNREQDDSL VLVWQNSGIW EKFVILEKEQ QDSSKTHYSL 430 440 NESLRNEDTK SAGEPTRWEL VRESWREL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed: 8701610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Functional analysis of the Saccharomyces cerevisiae YFR021w/YGR223c/YPL100w ORF family suggests relations to mitochondrial/peroxisomal functions and amino acid signalling pathways." Georgakopoulos T., Koutroubas G., Vakonakis I., Tzermia M., Prokova V., Voutsina A., Alexandraki D. Yeast 18:1155-1171(2001) [PubMed: 11536337] [Abstract] Cited for: FUNCTION.
[4]	"Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors." Dove S.K., Piper R.C., McEwen R.K., Yu J.W., King M.C., Hughes D.C., Thuring J., Holmes A.B., Cooke F.T., Michell R.H., Parker P.J., Lemmon M.A. EMBO J. 23:1922-1933(2004) [PubMed: 15103325] [Abstract] Cited for: INTERACTION WITH PIP2, SUBCELLULAR LOCATION.
[5]	"Dissecting the localization and function of Atg18, Atg21 and Ygr223c." Krick R., Henke S., Tolstrup J., Thumm M. Autophagy 4:896-910(2008) [PubMed: 18769150] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed: 19756047] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X87941 Genomic DNA. Translation: CAA61171.1. Z73008 Genomic DNA. Translation: CAA97251.1.
PIR	S57686.
RefSeq	NP_011739.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5556N.
IntAct	P50079. 7 interactions.
STRING	P50079.
Proteomic databases
PeptideAtlas	P50079.
PRIDE	P50079.
Genome annotation databases
Ensembl	YGR223C; YGR223C; YGR223C; Saccharomyces cerevisiae. [Genome view]
GeneID	853138.
KEGG	sce:YGR223C.
NMPDR	fig|4932.3.peg.2867.
Organism-specific databases
CYGD	YGR223c.
SGD	S000003455. HSV2.
Phylogenomic databases
HOGENOM	P50079.
OMA	MLYRTNY
OrthoDB	EOG976MH7
Gene expression databases
ArrayExpress	P50079.
Genevestigator	P50079.
GermOnline	YGR223C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
SMART	SM00320. WD40. 2 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973204.

Entry information

Entry name

HSV2_YEAST

Accession

Primary (citable) accession number: P50079

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)