Reviewed, UniProtKB/Swiss-Prot P50076 (ALG10_YEAST)

Names and origin

Protein names

Recommended name:
    Alpha-1,2 glucosyltransferase ALG10
    EC=2.4.1.-
Alternative name(s):
    Alpha-2-glucosyltransferase ALG10
    Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10
    Asparagine-linked glycosylation protein 10

Gene names

Name:	DIE2
Synonyms:	ALG10
Ordered Locus Names:	YGR227W
ORF Names:	G8547

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. Ref.4
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ALG10 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dolichol-linked oligosaccharide biosynthetic process Traceable author statement. Source: SGD
Cellular component	endoplasmic reticulum membrane Ref.4 Inferred from mutant phenotype. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Alpha-1,2 glucosyltransferase ALG10		PRO_0000215463
Regions
	Transmembrane	36 – 56	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	128 – 148	21	Potential
	Transmembrane	151 – 171	21	Potential
	Transmembrane	188 – 208	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	257 – 277	21	Potential
	Transmembrane	295 – 315	21	Potential
	Transmembrane	334 – 354	21	Potential
	Transmembrane	376 – 396	21	Potential
	Transmembrane	424 – 444	21	Potential
	Transmembrane	448 – 468	21	Potential
	Transmembrane	493 – 513	21	Potential
Amino acid modifications
	Glycosylation	31	1	N-linked (GlcNAc...) Potential
	Glycosylation	278	1	N-linked (GlcNAc...) Potential
	Glycosylation	372	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	266	1	K → I in BAA07239. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50076-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 40451CE29F44AB6F	FASTA	525	61,808
	10 20 30 40 50 60 MDAKKNTGEA NNDVLEEEAA IQLIAPGIAR NLTQEVITGI FCNVVIYPLL LIYFVLTFRY 70 80 90 100 110 120 MTTNIVPYEF IDEKFHVGQT LTYLKGKWTQ WDPKITTPPG IYILGLINYY CIKPIFKSWS 130 140 150 160 170 180 TLTILRLVNL LGGIIVFPIL VLRPIFLFNA LGFWPVSLMS FPLMTTYYYL FYTDVWSTIL 190 200 210 220 230 240 ILQSLSCVLT LPFGPVKSIW LSAFFAGVSC LFRQTNIIWT GFIMILAVER PAILQKQFNT 250 260 270 280 290 300 HTFNNYLKLF IHAIDDFSNL VLPYMKNFVL FFIYLIWNRS ITLGDKSSHS AGLHIVQIFY 310 320 330 340 350 360 CFTFITVFSL PIWISRNFMK LYKLRIKRKP VQTFFEFIGI MLIIRYFTKV HPFLLADNRH 370 380 390 400 410 420 YTFYLFRRLI GNKSRLIKYF FMTPIYHFST FAYLEVMRPN QLTFHPITPL PIKEPVHLPI 430 440 450 460 470 480 QLTHVSWTAL ITCTMVTIVP SPLFEPRYYI LPYFFWRIFI TCSCEPLIKD LKPAKEGENP 490 500 510 520 ITISSTKRLF MEFLWFMLFN VVTLVIFSKV SFPWTTEPYL QRIIW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae." Nikawa J., Hosaka K. Mol. Microbiol. 16:301-308(1995) [PubMed: 7565092] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed: 8701610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation." Burda P., Aebi M. Glycobiology 8:455-462(1998) [PubMed: 9597543] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38049 Genomic DNA. Translation: BAA07239.1. X87941 Genomic DNA. Translation: CAA61176.1. Z73012 Genomic DNA. Translation: CAA97255.1.
PIR	S57691.
RefSeq	NP_011743.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P50076. 2 interactions.
Protein family/group databases
CAZy	GT59. Glycosyltransferase Family 59.
Genome annotation databases
Ensembl	YGR227W. Saccharomyces cerevisiae. [Contig view]
GeneID	853142.
GenomeReviews	Gene locus YGR227W in contig Y13135_GR.
KEGG	sce:YGR227W.
NMPDR	fig|4932.3.peg.2871.
Organism-specific databases
CYGD	YGR227w.
SGD	S000003459. DIE2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P50076.
OMA	P50076. WKFTYVH.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-7193.
Gene expression databases
GermOnline	YGR227W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016900. Alpha1_2_glucosyltferase_Alg10. IPR007006. Glycosyltransferase_ALG10. [Graphical view]
PANTHER	PTHR12989. DIE2_ALG10. 1 hit.
Pfam	PF04922. DIE2_ALG10. 1 hit. [Graphical view]
PIRSF	PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	973213.

Entry information

Entry name

ALG10_YEAST

Accession

Primary (citable) accession number: P50076

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)