Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

DIE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc2-PP-Dol.1 Publication

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: SGD
  • protein N-linked glycosylation Source: SGD

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YGR227W-MONOMER
YEAST:YGR227W-MONOMER
BRENDAi2.4.1.256 984
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT59 Glycosyltransferase Family 59
TCDBi9.B.231.1.1 the die2/alg10 glycosyl transferase (die2/alg10) family

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC:2.4.1.256)
Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10
Asparagine-linked glycosylation protein 10
Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10
Gene namesi
Name:DIE2
Synonyms:ALG10
Ordered Locus Names:YGR227W
ORF Names:G8547
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR227W
SGDiS000003459 DIE2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Transmembranei295 – 315HelicalSequence analysisAdd BLAST21
Transmembranei334 – 354HelicalSequence analysisAdd BLAST21
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Transmembranei493 – 513HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002154631 – 525Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi31N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi278N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi372N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP50076
PRIDEiP50076

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CREB3L1Q96BA83EBI-7301533,EBI-6942903From Homo sapiens.

Protein-protein interaction databases

BioGridi33480, 140 interactors
IntActiP50076, 7 interactors
MINTiP50076
STRINGi4932.YGR227W

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG10 glucosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000012906
HOGENOMiHOG000240902
InParanoidiP50076
KOiK03850
OMAiWWFRYAM
OrthoDBiEOG092C321K

Family and domain databases

InterProiView protein in InterPro
IPR016900 Alg10
PANTHERiPTHR12989 PTHR12989, 1 hit
PfamiView protein in Pfam
PF04922 DIE2_ALG10, 1 hit
PIRSFiPIRSF028810 Alpha1_2_glucosyltferase_Alg10, 1 hit

Sequencei

Sequence statusi: Complete.

P50076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAKKNTGEA NNDVLEEEAA IQLIAPGIAR NLTQEVITGI FCNVVIYPLL
60 70 80 90 100
LIYFVLTFRY MTTNIVPYEF IDEKFHVGQT LTYLKGKWTQ WDPKITTPPG
110 120 130 140 150
IYILGLINYY CIKPIFKSWS TLTILRLVNL LGGIIVFPIL VLRPIFLFNA
160 170 180 190 200
LGFWPVSLMS FPLMTTYYYL FYTDVWSTIL ILQSLSCVLT LPFGPVKSIW
210 220 230 240 250
LSAFFAGVSC LFRQTNIIWT GFIMILAVER PAILQKQFNT HTFNNYLKLF
260 270 280 290 300
IHAIDDFSNL VLPYMINFVL FFIYLIWNRS ITLGDKSSHS AGLHIVQIFY
310 320 330 340 350
CFTFITVFSL PIWISRNFMK LYKLRIKRKP VQTFFEFIGI MLIIRYFTKV
360 370 380 390 400
HPFLLADNRH YTFYLFRRLI GNKSRLIKYF FMTPIYHFST FAYLEVMRPN
410 420 430 440 450
QLTFHPITPL PIKEPVHLPI QLTHVSWTAL ITCTMVTIVP SPLFEPRYYI
460 470 480 490 500
LPYFFWRIFI TCSCEPLIKD LKPAKEGENP ITISSTKRLF MEFLWFMLFN
510 520
VVTLVIFSKV SFPWTTEPYL QRIIW
Length:525
Mass (Da):61,793
Last modified:September 21, 2011 - v2
Checksum:iCCA51CE249A4AB6E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266I → K in CAA61176 (PubMed:8701610).Curated1
Sequence conflicti266I → K in CAA97255 (PubMed:9169869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38049 Genomic DNA Translation: BAA07239.1
X87941 Genomic DNA Translation: CAA61176.1
Z73012 Genomic DNA Translation: CAA97255.1
BK006941 Genomic DNA Translation: DAA08319.2
PIRiS57691
RefSeqiNP_011743.2, NM_001181356.2

Genome annotation databases

EnsemblFungiiYGR227W; YGR227W; YGR227W
GeneIDi853142
KEGGisce:YGR227W

Similar proteinsi

Entry informationi

Entry nameiALG10_YEAST
AccessioniPrimary (citable) accession number: P50076
Secondary accession number(s): D6VV08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health