Reviewed, UniProtKB/Swiss-Prot P50075 (GYRBS_STRSH)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B, novobiocin-sensitive
    EC=5.99.1.3

Gene names

Name:

gyrBS

Organism

Streptomyces sphaeroides (Streptomyces caeruleus)

Taxonomic identifier

195949 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Miscellaneous	There are two genes for gyrB in this organism. One which is novobiocin-sensitive (gyrBS) and one which is resistant (gyrBR).
Sequence similarities	Belongs to the type II topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	DNA gyrase subunit B, novobiocin-sensitive		PRO_0000145354
Regions
	Domain	463 – 574	112	Toprim
	Region	154 – 302	149	Novobiocin-binding

Sequences

	Sequence		Length	Mass (Da)
	P50075-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: DBE5D8E546C68886	FASTA	684	75,246
	10 20 30 40 50 60 MADSGNPNEN TPSVATGENG EVTGSYNASA ITVLEGLDAV RKRPGMYIGS TGERGLHHLV 70 80 90 100 110 120 TEVVDNSVDE ALAGHADTID VTILADGGVR VVDNGRGIPV GIVPSEGKPA VEVVLTVLHA 130 140 150 160 170 180 GGKFGGGGYS VSGGLHGVGV SVVNALSTKV AVEVKTDGYR WTQDYKLGVP TRRCAQNEAT 190 200 210 220 230 240 DETGTTVTFW ADPDVFETTE YSFETLSRRF QEMAFLNKGL TLKLTDERES AKAVVGADVA 250 260 270 280 290 300 GTDSAETPGE EPVRSVTYYY EGGIVDFVKY LNSRKGDLIH PTVIDIDAED KERMLSVEIA 310 320 330 340 350 360 MQWNSQYSEG VYSFANTIHT HEGGTHEEGF RAAMTGLVNR YAREKKFLRE KDDNLAGEDI 370 380 390 400 410 420 REGLTAIISV KLGEPQFEGQ TKTKLGNTEA KTFVQKIVHE HLTDWFDRHP NEAADIIRKS 430 440 450 460 470 480 IQAATARVAA RKARDLTRRK GLLESASLPG KLSDCQSNDP SKCEIFIVEG DSAGGSAKSG 490 500 510 520 530 540 RNPQYQAILP IRGKILNVEK ARIDKILQNT EVQALISAFG TGVHEDFDIE KLRYHKIILM 550 560 570 580 590 600 ADADVDGQHI NTLLLTFLFR FMRPLVEAGH VYLSRPPLYK IKWGRDDFEY AYSDRERDAL 610 620 630 640 650 660 VELGKQNGKR IKEDSIQRFK GLGEMNAEEL RITTMDVDHR VLGQVTLDDA AQADDLFSVL 670 680 MGEDVEARRS FIQRNAKDVR FLDI

References

[1]

"Expression and analysis of two gyrB genes from the novobiocin producer, Streptomyces sphaeroides." Thiara A.S., Cundliffe E. Mol. Microbiol. 8:495-506(1993) [PubMed: 8392138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23965 / DSM 40292 / IFO 12917 / JCM 4252 / NCIMB 11891.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z17305 Genomic DNA. Translation: CAA78952.1.
PIR	S29683.
3D structure databases
SMR	P50075. Positions 30-436, 451-641.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 2349.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01059. gyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRBS_STRSH

Accession

Primary (citable) accession number: P50075

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)