P50071 (GYRA_STRSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Streptomyces spheroides (Streptomyces caeruleus)

Taxonomic identifier

195949 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	21 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›21	›21	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145268
Experimental info
	Non-terminal residue	21	1

Sequences

	Sequence		Length	Mass (Da)
	P50071 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5E2F9DEA8DB83697	FASTA	21	2,202
	10 20 MADENTPVMP EEVPAVEGVG M

References

[1]

"Expression and analysis of two gyrB genes from the novobiocin producer, Streptomyces sphaeroides." Thiara A.S., Cundliffe E. Mol. Microbiol. 8:495-506(1993) [PubMed: 8392138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL 2449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z17305 Genomic DNA. Translation: CAA78953.1.
PIR	S32883.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
ProtoNet	Search...

Entry information

Entry name

GYRA_STRSH

Accession

Primary (citable) accession number: P50071

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 27, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program