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P50049 (UREE_BACPA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Urease accessory protein UreE
Gene names
Name:ureE
OrganismBacillus pasteurii
Taxonomic identifier1474 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Planococcaceae › Sporosarcina

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in urease metallocenter assembly. Binds nickel. Probably functions as a nickel donor during metallocenter assembly. HAMAP MF_00822

Cofactor

Binds 1 Zn2+ or 1 Ni2+ ion per dimer or tetramer. Ref.2

Subunit structure

Homodimer in diluted solutions, homotetramer in concentrated solutions and when bound to Ni2+ or Zn2+. The homodimer is thought to be the active form. Ref.2

Subcellular location

Cytoplasm By similarity HAMAP MF_00822.

Sequence similarities

Belongs to the UreE family.

Sequence caution

The sequence AAA73987.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNickel insertion
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
Zinc
   Molecular functionChaperone
   Technical term3D-structure
Gene Ontology (GO)
   Biological processenzyme active site formation

Inferred from electronic annotation. Source: InterPro

protein complex assembly

Inferred from electronic annotation. Source: InterPro

protein folding

Inferred from electronic annotation. Source: InterPro

urea metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147Urease accessory protein UreE HAMAP MF_00822
PRO_0000067629

Sites

Metal binding1001Nickel

Secondary structure

1 ........................... 147
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50049 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 8E07578B67230DD3

FASTA14717,402
        10         20         30         40         50         60 
MLITKIVGHI DDYESSDKKV DWLEVEWEDL NKRILRKETE NGTDIAIKLE NSGTLRYGDV 

        70         80         90        100        110        120 
LYESDDTLIA IRTKLEKVYV IKPQTMQEMG KMAFEIGNRH TMCIIEDDEI LVRYDKTLEK 

       130        140 
LIDEVGVSYE QSERRFKEPF KYRGHQH

« Hide

References

[1]"Genetic organization and nucleotide sequence of the ure gene cluster in Bacillus pasteurii."
You J.-H., Kim J.-G., Song B.-H., Lee M.-H., Kim S.-D.
Mol. Cells 5:359-369(1995)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822.
[2]"Molecular characterization of Bacillus pasteurii UreE, a metal-binding chaperone for the assembly of the urease active site."
Ciurli S., Safarov N., Miletti S., Dikiy A., Christensen S.K., Kornetzky K., Bryant D.A., Vandenberghe I., Devreese B., Samyn B., Remaut H., Van Beeumen J.
J. Biol. Inorg. Chem. 7:623-631(2002) [PubMed: 12072968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], COFACTOR, SUBUNIT, MASS SPECTROMETRY.
Strain: ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822.
[3]"Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii."
Remaut H., Safarov N., Ciurli S., Van Beeumen J.
J. Biol. Chem. 276:49365-49370(2001) [PubMed: 11602602] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U29368 Genomic DNA. Translation: AAA73987.1. Different initiation.
AF361945 Genomic DNA. Translation: AAD55059.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EARX-ray1.70A3-147[»]
1EB0X-ray1.85A3-147[»]
ProteinModelPortalP50049.
SMRP50049. Positions 1-143.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_00822. UreE.
[Tree]
InterProIPR012406. UreE.
IPR007864. UreE_C.
IPR004029. UreE_N.
[Graphical view]
Gene3DG3DSA:3.30.70.790. UreE_C. 1 hit.
PfamPF05194. UreE_C. 1 hit.
PF02814. UreE_N. 1 hit.
[Graphical view]
PIRSFPIRSF036402. Ureas_acces_UreE. 1 hit.
SMARTSM00988. UreE_N. 1 hit.
[Graphical view]
SUPFAMSSF69737. UreE_C. 1 hit.
SSF69287. UreE_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameUREE_BACPA
AccessionPrimary (citable) accession number: P50049
Secondary accession number(s): Q9RP20
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: January 25, 2012
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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