P50045 (URE1_HELMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit beta
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit beta

Gene names

Name:

ureB

Organism

Helicobacter mustelae

Taxonomic identifier

217 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	308 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity. HAMAP MF_01953
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01953.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity. HAMAP MF_01953
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.
Caution	The orthologous protein is known as the alpha subunit (UreC) in most other bacteria.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›308	›308	Urease subunit beta HAMAP MF_01953		PRO_0000067532
Regions
	Domain	131 – ›308	›178	Urease
Sites
	Metal binding	136	1	Nickel 2 By similarity
	Metal binding	138	1	Nickel 2 By similarity
	Metal binding	219	1	Nickel 1; via carbamate group By similarity
	Metal binding	219	1	Nickel 2; via carbamate group By similarity
	Metal binding	248	1	Nickel 1 By similarity
	Metal binding	274	1	Nickel 1 By similarity
Amino acid modifications
	Modified residue	219	1	N6-carboxylysine By similarity
Experimental info
	Non-terminal residue	308	1

Sequences

	Sequence		Length	Mass (Da)
	P50045 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1F11F29A5B3C84B1	FASTA	308	32,118
	10 20 30 40 50 60 MIKISRKEYV SMYGPTTGDK VRLGDTELIA EIEKDYTVYG EEIKFGGGKT IRDGMSQSVS 70 80 90 100 110 120 PDVNELDAVI TNAMIIDYTG IYKADIGIKD GKIAGIGKAG NRDTQDGVGM DLVVGASTEA 130 140 150 160 170 180 IAGEGLIVTA GGIDTHIHFI SPTQIPTALY SGVTTMIGGG TGPAAGTFAT TISPGEWNIK 190 200 210 220 230 240 QMIRAAEEYT MNLGFFGKGN TSNVKALEDQ IKAGALGFKV HEDCGSTPAV INHSLDIAEK 250 260 270 280 290 300 YDVQVAIHTD TLNEGGAVED TLAAIGGRTI HTFHTEGAGG GHAPDIIKAA GEANILPAST NPTIPFTK

References

[1]	"Construction and characterization of an isogenic urease-negative mutant of Helicobacter mustelae." Solnick J.V., Josenhans C., Tompkins L.S., Labigne A. Infect. Immun. 63:3718-3721(1995) [PubMed: 7642313] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 12032.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33462 Genomic DNA. Translation: AAC41484.1.
3D structure databases
ProteinModelPortal	P50045.
SMR	P50045. Positions 1-307.
ModBase	Search...
Protein family/group databases
MEROPS	M38.982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01953. Urease_alpha. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011059. Metal-dep_hydrolase_composite. IPR011612. Urease_alpha_N. IPR017951. Urease_asu_c. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. Partial match. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_HELMU

Accession

Primary (citable) accession number: P50045

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program