P50019 (DNAK_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein DnaK

Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70

Gene names

Name:	dnaK
Ordered Locus Names:	Atu0122
ORF Names:	AGR_C_195

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a chaperone By similarity. HAMAP-Rule MF_00332
Induction	By heat shock. HAMAP-Rule MF_00332
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	Chaperone protein DnaK HAMAP-Rule MF_00332		PRO_0000078405
Amino acid modifications
	Modified residue	198	1	Phosphothreonine; by autocatalysis By similarity
Experimental info
	Sequence conflict	293	1	L → P in CAA60592. Ref.1
	Sequence conflict	345	1	Q → R in CAA60592. Ref.1
	Sequence conflict	463	1	G → A in CAA60592. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50019 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: D53CC7E6EB3B50E3	FASTA	633	68,080
	10 20 30 40 50 60 MAKVIGIDLG TTNSCVAVMD GKDTKVIENA EGARTTPSMV AFSDDGERLV GQPAKRQAVT 70 80 90 100 110 120 NPTNTLFAVK RLIGRRYEDP TVEKDKALVP FEIVKGDNGD AWVKAQDKNY SPSQISAMIL 130 140 150 160 170 180 QKMKETAESY LGEKVEKAVI TVPAYFNDAQ RQATKDAGRI AGLDVLRIIN EPTAAALAYG 190 200 210 220 230 240 LDKKEGKTIA VYDLGGGTFD ISVLEIGDGV FEVKSTNGDT FLGGEDFDMR LVEYLAGEFK 250 260 270 280 290 300 KDQGIDLKND KLALQRLKEA AEKAKIELSS SQQTEINLPF ITADASGPKH LTLKLTRAKF 310 320 330 340 350 360 ESLVDDLVQR TVAPCKAALK DAGVTAAEID EVVLVGGMSR MPKVQEVVKQ LFGKEPHKGV 370 380 390 400 410 420 NPDEVVAMGA AIQAGVLQGD VKDVLLLDVT PLSLGIETLG GVFTRLIDRN TTIPTKKSQT 430 440 450 460 470 480 FSTAEDNQSA VTIRVSQGER EMAQDNKLLG QFDLVGLPPS PRGVPQIEVT FDIDANGIVQ 490 500 510 520 530 540 VSAKDKGTGK EQQIRIQASG GLSDADIEKM VKDAEANAEA DKKRRAGVEA KNQAESLIHS 550 560 570 580 590 600 TEKSVKEYGD KVSETDRKAI EDAIASLKTA VEAAEPDADD IQAKTQTLME VSMKLGQAIY 610 620 630 EAQQAEAGDA SAEGKDDVVD ADYEEIKDDK KSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The dnaKJ operon of Agrobacterium tumefaciens: transcriptional analysis and evidence for a new heat shock promoter." Segal G., Ron E.Z. J. Bacteriol. 177:5952-5958(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[3]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87113 Genomic DNA. Translation: CAA60592.1. AE007869 Genomic DNA. Translation: AAK85942.1.
PIR	AE2591. E97373. I39585.
RefSeq	NP_353157.1. NC_003062.2.
3D structure databases
ProteinModelPortal	P50019.
SMR	P50019. Positions 1-595.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu0122.
Proteomic databases
PRIDE	P50019.
ProMEX	P50019.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1132160.
KEGG	atu:Atu0122.
PATRIC	20809924. VBIAgrTum91616_0114.
Phylogenomic databases
eggNOG	COG0443.
HOGENOM	HOG000228136.
KO	K04043.
OMA	NSCLAIS.
ProtClustDB	PRK00290.
Family and domain databases
HAMAP	MF_00332. DnaK.
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAK_AGRT5

Accession

Primary (citable) accession number: P50019

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 27, 2002
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program