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P50019 (DNAK_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chaperone protein DnaK
Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70
Gene names
Name:dnaK
Ordered Locus Names:Atu0122
ORF Names:AGR_C_195
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length633 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a chaperone By similarity. HAMAP-Rule MF_00332

Induction

By heat shock. HAMAP-Rule MF_00332

Sequence similarities

Belongs to the heat shock protein 70 family.

Ontologies

Keywords
   Biological processStress response
   LigandATP-binding
Nucleotide-binding
   Molecular functionChaperone
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 633633Chaperone protein DnaK HAMAP-Rule MF_00332
PRO_0000078405

Amino acid modifications

Modified residue1981Phosphothreonine; by autocatalysis By similarity

Experimental info

Sequence conflict2931L → P in CAA60592. Ref.1
Sequence conflict3451Q → R in CAA60592. Ref.1
Sequence conflict4631G → A in CAA60592. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P50019 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: D53CC7E6EB3B50E3

FASTA63368,080
        10         20         30         40         50         60 
MAKVIGIDLG TTNSCVAVMD GKDTKVIENA EGARTTPSMV AFSDDGERLV GQPAKRQAVT 

        70         80         90        100        110        120 
NPTNTLFAVK RLIGRRYEDP TVEKDKALVP FEIVKGDNGD AWVKAQDKNY SPSQISAMIL 

       130        140        150        160        170        180 
QKMKETAESY LGEKVEKAVI TVPAYFNDAQ RQATKDAGRI AGLDVLRIIN EPTAAALAYG 

       190        200        210        220        230        240 
LDKKEGKTIA VYDLGGGTFD ISVLEIGDGV FEVKSTNGDT FLGGEDFDMR LVEYLAGEFK 

       250        260        270        280        290        300 
KDQGIDLKND KLALQRLKEA AEKAKIELSS SQQTEINLPF ITADASGPKH LTLKLTRAKF 

       310        320        330        340        350        360 
ESLVDDLVQR TVAPCKAALK DAGVTAAEID EVVLVGGMSR MPKVQEVVKQ LFGKEPHKGV 

       370        380        390        400        410        420 
NPDEVVAMGA AIQAGVLQGD VKDVLLLDVT PLSLGIETLG GVFTRLIDRN TTIPTKKSQT 

       430        440        450        460        470        480 
FSTAEDNQSA VTIRVSQGER EMAQDNKLLG QFDLVGLPPS PRGVPQIEVT FDIDANGIVQ 

       490        500        510        520        530        540 
VSAKDKGTGK EQQIRIQASG GLSDADIEKM VKDAEANAEA DKKRRAGVEA KNQAESLIHS 

       550        560        570        580        590        600 
TEKSVKEYGD KVSETDRKAI EDAIASLKTA VEAAEPDADD IQAKTQTLME VSMKLGQAIY 

       610        620        630 
EAQQAEAGDA SAEGKDDVVD ADYEEIKDDK KSA 

« Hide

References

« Hide 'large scale' references
[1]"The dnaKJ operon of Agrobacterium tumefaciens: transcriptional analysis and evidence for a new heat shock promoter."
Segal G., Ron E.Z.
J. Bacteriol. 177:5952-5958(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome of the natural genetic engineer Agrobacterium tumefaciens C58."
Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. expand/collapse author list , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W.
Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C58 / ATCC 33970.
[3]"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58."
Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. expand/collapse author list , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.
Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C58 / ATCC 33970.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87113 Genomic DNA. Translation: CAA60592.1.
AE007869 Genomic DNA. Translation: AAK85942.1.
PIRAE2591.
E97373.
I39585.
RefSeqNP_353157.1. NC_003062.2.

3D structure databases

ProteinModelPortalP50019.
SMRP50019. Positions 1-595.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu0122.

Proteomic databases

PRIDEP50019.
ProMEXP50019.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK85942; AAK85942; Atu0122.
GeneID1132160.
KEGGatu:Atu0122.
PATRIC20809924. VBIAgrTum91616_0114.

Phylogenomic databases

eggNOGCOG0443.
HOGENOMHOG000228136.
KOK04043.
OMAKIQANSG.
OrthoDBEOG6JMMSV.

Enzyme and pathway databases

BioCycAGRO:ATU0122-MONOMER.

Family and domain databases

Gene3D1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPMF_00332. DnaK.
InterProIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
SUPFAMSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsTIGR02350. prok_dnaK. 1 hit.
PROSITEPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAK_AGRT5
AccessionPrimary (citable) accession number: P50019
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 27, 2002
Last modified: June 11, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families