P49997 (RECF_AZOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication and repair protein recF

Gene names

Name:

recF

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. HAMAP MF_00365
Subcellular location	Cytoplasm Potential HAMAP MF_00365.
Sequence similarities	Belongs to the recF family.
Caution	This entry seems to be produced by an incorrect sequence that contains at least 14 frameshifts. Do not use it for any phylogenetic purpose.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW single-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	DNA replication and repair protein recF HAMAP MF_00365		PRO_0000196393
Regions
	Nucleotide binding	43 – 50	8	ATP Potential
	Compositional bias	234 – 259	26	Arg-rich (basic) HAMAP MF_00365
	Compositional bias	241 – 244	4	Poly-Arg HAMAP MF_00365

Sequences

	Sequence		Length	Mass (Da)
	P49997 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BFF65FDB63AC8407	FASTA	364	42,016
	10 20 30 40 50 60 MYGFRLCRPC RCGCKPATPP RCPSAVTVTR CVTSARVNQY ISGPNGSGKR PAYWRRSTCS 70 80 90 100 110 120 AWQLSSFRSQ RLSPVIQHEQ PACTVFGQVL WNDGRVRNLG VARNRLGELQ IRIDGQNVRS 130 140 150 160 170 180 AAQLAESLPL QLINPDSFRL LEGAPKVRRQ FLDWGVFHVE QRFLPAWDRL QTALRQRNSW 190 200 210 220 230 240 LRHGRIDPVS QAAWDRELCL RRSDSELLRA GRERCSARFL LLPFRAISNW GIPRRPQRAD 250 260 270 280 290 300 RRRRLARTMP RTCCRAVTRS WWSVRLKIAQ GHLVDRARRE CIYLVDDLPS ELDEQHRRAL 310 320 330 340 350 360 CRLLEELHCQ VFITCVDLEA LREGWRTDTP VALFHVNRAL ITQPTIGSEG NDRKPTYDSS SIKS

References

[1]	"Molecular characterization of the Azotobacter vinelandii recF gene." Badran H., Venkatesh T.V., Kunnimalaiyaan M., Sharma N., Das H.K. Gene 162:47-51(1995) [PubMed: 7557415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86404 Genomic DNA. Translation: CAA60158.1.
PIR	JC4249.
3D structure databases
ProteinModelPortal	P49997.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00365. RecF. Divergent sequence. [Tree]
InterPro	IPR001238. DNA-binding_RecF. IPR018078. DNA-binding_RecF_CS. IPR003395. RecF/RecN/SMC. [Graphical view]
Pfam	PF02463. SMC_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00611. Recf. 1 hit.
PROSITE	PS00617. RECF_1. 1 hit. PS00618. RECF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECF_AZOVI

Accession

Primary (citable) accession number: P49997

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program