P49995 (DNAA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	sll0848

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP-Rule MF_00377
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Inferred from electronic annotation. Source: HAMAP regulation of DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA replication origin binding Inferred from electronic annotation. Source: HAMAP nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Chromosomal replication initiator protein DnaA HAMAP-Rule MF_00377		PRO_0000114285
Regions
	Nucleotide binding	147 – 154	8	ATP Potential
Experimental info
	Sequence conflict	118 – 125	8	VGPTNRMA → WAHQSHG in AAA85913. Ref.1
	Sequence conflict	297	1	I → M in AAA85913. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49995 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8ACA68D6CC38D3BF	FASTA	447	50,050
	10 20 30 40 50 60 MVSCENLWQQ ALAILATQLT KPAFDTWIKA SVLISLGDGV ATIQVENGFV LNHLQKSYGP 70 80 90 100 110 120 LLMEVLTDLT GQEITVKLIT DGLEPHSLIG QESSLPMETT PKNATALNGK YTFSRFVVGP 130 140 150 160 170 180 TNRMAHAASL AVAESPGREF NPLFLCGGVG LGKTHLMQAI AHYRLEMYPN AKVYYVSTER 190 200 210 220 230 240 FTNDLITAIR QDNMEDFRSY YRSADFLLID DIQFIKGKEY TQEEFFHTFN SLHEAGKQVV 250 260 270 280 290 300 VASDRAPQRI PGLQDRLISR FSMGLIADIQ VPDLETRMAI LQKKAEYDRI RLPKEVIEYI 310 320 330 340 350 360 ASHYTSNIRE LEGALIRAIA YTSLSNVAMT VENIAPVLNP PVEKVAAAPE TIITIVAQHY 370 380 390 400 410 420 QLKVEELLSN SRRREVSLAR QVGMYLMRQH TDLSLPRIGE AFGGKDHTTV MYSCDKITQL 430 440 QQKDWETSQT LTSLSHRINI AGQAPES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic structure of the dnaA region of the cyanobacterium Synechocystis sp. strain PCC6803." Richter S., Messer W. J. Bacteriol. 177:4245-4251(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36958 Genomic DNA. Translation: AAA85913.1. BA000022 Genomic DNA. Translation: BAA17801.1.
PIR	S74840.
RefSeq	NP_441121.1. NC_000911.1. YP_005651178.1. NC_017277.1. YP_007451003.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P49995.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49995. 2 interactions.
STRING	1148.sll0848.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17801; BAA17801; BAA17801.
GeneID	12255505. 14616658. 954435.
KEGG	syn:sll0848. syy:SYNGTS_1225.
PATRIC	23839547. VBISynSp132158_1336.
Phylogenomic databases
eggNOG	COG0593.
HOGENOM	HOG000235658.
KO	K02313.
OMA	CHTLNAL.
ProtClustDB	PRK00149.
Family and domain databases
Gene3D	1.10.1750.10. 1 hit.
HAMAP	MF_00377. DnaA_bact.
InterPro	IPR003593. AAA+_ATPase. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR024633. DnaA_N_dom. IPR027417. P-loop_NTPase. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. PF11638. DnaA_N. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_SYNY3

Accession

Primary (citable) accession number: P49995
Secondary accession number(s): P73750

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program