Chromosomal replication initiator protein DnaA

Contribute

Send feedback

Read comments (?) or add your own

P49993 (DNAA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	Rv0001, MT0001
ORF Names:	MTV029.01

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro protein homooligomerization Inferred from physical interaction. Source: MTBBASE regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from direct assay. Source: MTBBASE cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	ATP binding Inferred from direct assay. Source: MTBBASE ATPase activity Inferred from direct assay. Source: MTBBASE DNA replication origin binding Inferred from direct assay. Source: MTBBASE protein binding Inferred from physical interaction. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 507

507

Chromosomal replication initiator protein DnaA HAMAP MF_00377

PRO_0000114218

Regions

Nucleotide binding

208 – 215

ATP Potential

Experimental info

Sequence conflict

363

S → R in AAA85543. Ref.5

Sequence conflict

371

E → V in AAA85543. Ref.5

Sequence conflict

381

A → P in AAA85543. Ref.5

Sequence conflict

429 – 430

EE → GR in AAA85543. Ref.5

Sequence conflict

448

M → V in AAB38528. Ref.1

Sequence conflict

458

S → P in AAA85543. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P49993 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: C4F252150D453A00
Show »

FASTA

507

56,567

        10         20         30         40         50         60 
MTDDPGSGFT TVWNAVVSEL NGDPKVDDGP SSDANLSAPL TPQQRAWLNL VQPLTIVEGF 

        70         80         90        100        110        120 
ALLSVPSSFV QNEIERHLRA PITDALSRRL GHQIQLGVRI APPATDEADD TTVPPSENPA 

       130        140        150        160        170        180 
TTSPDTTTDN DEIDDSAAAR GDNQHSWPSY FTERPHNTDS ATAGVTSLNR RYTFDTFVIG 

       190        200        210        220        230        240 
ASNRFAHAAA LAIAEAPARA YNPLFIWGES GLGKTHLLHA AGNYAQRLFP GMRVKYVSTE 

       250        260        270        280        290        300 
EFTNDFINSL RDDRKVAFKR SYRDVDVLLV DDIQFIEGKE GIQEEFFHTF NTLHNANKQI 

       310        320        330        340        350        360 
VISSDRPPKQ LATLEDRLRT RFEWGLITDV QPPELETRIA ILRKKAQMER LAVPDDVLEL 

       370        380        390        400        410        420 
IASSIERNIR ELEGALIRVT AFASLNKTPI DKALAEIVLR DLIADANTMQ ISAATIMAAT 

       430        440        450        460        470        480 
AEYFDTTVEE LRGPGKTRAL AQSRQIAMYL CRELTDLSLP KIGQAFGRDH TTVMYAQRKI 

       490        500 
LSEMAERREV FDHVKELTTR IRQRSKR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The dnaA gene region of M. tuberculosis." Qin M.H., Madiraju M.V., Rajagopalan M. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis." Salazar L., Fsihi H., De Rossi E., Riccardi G., Rios C., Cole S.T., Takiff H.E. Mol. Microbiol. 20:283-293(1996) [PubMed: 8733228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[4]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[5]	"Amplification and cloning of the Mycobacterium tuberculosis dnaA gene." Rajagopalan M., Qin M.H., Steingrube V.A., Nash D.R., Wallace R.J. Jr., Madiraju M.V.V.S. Gene 163:75-79(1995) [PubMed: 7557482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 213-472.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38891 Genomic DNA. Translation: AAB38528.2. X92504 Genomic DNA. Translation: CAA63257.1. BX842572 Genomic DNA. Translation: CAA16238.1. AE000516 Genomic DNA. Translation: AAK44224.1. U19184 Genomic DNA. Translation: AAA85543.1.
PIR	S70982.
RefSeq	NP_214515.1. NC_000962.2. NP_334410.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P49993.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001037; EBMYCP00000001037; EBMYCG00000001037. EBMYCT00000072864; EBMYCP00000070923; EBMYCG00000072859.
GeneID	885041. 922429.
GenomeReviews	Gene locus MT0001 in contig AE000516_GR. Gene locus Rv0001 in contig AL123456_GR.
KEGG	mtc:MT0001. mtu:Rv0001.
PATRIC	18121726. VBIMycTub22151_0001.
TIGR	MT0001.
Organism-specific databases
TubercuList	Rv0001.
Phylogenomic databases
GeneTree	EBGT00050000017320.
HOGENOM	HBG695666.
OMA	ASVHESW.
PhylomeDB	P49993.
ProtClustDB	PRK00149.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_MYCTU

Accession

Primary (citable) accession number: P49993
Secondary accession number(s): P95309, Q59585

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1998
Last modified:	January 25, 2012
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents