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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi386 – 40520H-T-H motifSequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • DNA-templated transcription, initiation Source: InterPro
  • negative regulation of single-species biofilm formation Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Sigma factor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Ordered Locus Names:PA4462
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4462.

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497RNA polymerase sigma-54 factorPRO_0000205534Add
BLAST

Interactioni

Protein-protein interaction databases

MINTiMINT-8407298.
STRINGi208964.PA4462.

Structurei

3D structure databases

ProteinModelPortaliP49988.
SMRiP49988. Positions 417-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi474 – 4829RPON box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 3525Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiCOG1508.
HOGENOMiHOG000071905.
InParanoidiP49988.
KOiK03092.
OMAiKVTRCIV.
OrthoDBiEOG60CWN7.
PhylomeDBiP49988.

Family and domain databases

InterProiIPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
[Graphical view]
PfamiPF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
[Graphical view]
PIRSFiPIRSF000774. RpoN. 1 hit.
PRINTSiPR00045. SIGMA54FCT.
TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
PROSITEiPS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ
60 70 80 90 100
EDGDDFDNSD PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS
110 120 130 140 150
ELPVDTAWED IYQTSASSLP SNDDDEWDFT ARTSSGESLH SHLLWQVNLA
160 170 180 190 200
PMSDTDRMIA VTIIDSINND GYLEESLEEI LAAIDPELDV ELDEVEVVLR
210 220 230 240 250
RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV SDYLDLLGGR
260 270 280 290 300
DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK
310 320 330 340 350
DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI
360 370 380 390 400
KSLQSRNETL MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST
410 420 430 440 450
ISRVTTQKYM HTPRGIFELK YFFSSHVSTA EGGECSSTAI RAIIKKLVAA
460 470 480 490
ENAKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLV
Length:497
Mass (Da):56,054
Last modified:December 8, 2000 - v2
Checksum:iCE1994F03FD58405
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521S → T in AAA19793 (PubMed:8113171).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26916 Unassigned DNA. Translation: AAA19793.1.
AE004091 Genomic DNA. Translation: AAG07850.1.
PIRiA53373.
F83087.
RefSeqiNP_253152.1. NC_002516.2.
WP_003094357.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07850; AAG07850; PA4462.
GeneIDi881022.
KEGGipae:PA4462.
PATRICi19843651. VBIPseAer58763_4672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26916 Unassigned DNA. Translation: AAA19793.1.
AE004091 Genomic DNA. Translation: AAG07850.1.
PIRiA53373.
F83087.
RefSeqiNP_253152.1. NC_002516.2.
WP_003094357.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP49988.
SMRiP49988. Positions 417-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8407298.
STRINGi208964.PA4462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07850; AAG07850; PA4462.
GeneIDi881022.
KEGGipae:PA4462.
PATRICi19843651. VBIPseAer58763_4672.

Organism-specific databases

PseudoCAPiPA4462.

Phylogenomic databases

eggNOGiCOG1508.
HOGENOMiHOG000071905.
InParanoidiP49988.
KOiK03092.
OMAiKVTRCIV.
OrthoDBiEOG60CWN7.
PhylomeDBiP49988.

Family and domain databases

InterProiIPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
[Graphical view]
PfamiPF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
[Graphical view]
PIRSFiPIRSF000774. RpoN. 1 hit.
PRINTSiPR00045. SIGMA54FCT.
TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
PROSITEiPS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa."
    Jin S., Ishimoto K., Lory S.
    J. Bacteriol. 176:1316-1322(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PAK.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRP54_PSEAE
AccessioniPrimary (citable) accession number: P49988
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: May 27, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.