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Protein

Signal recognition particle 54 kDa protein 2

Gene

SRP-54B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 11710GTPBy similarity
Nucleotide bindingi192 – 1965GTPBy similarity
Nucleotide bindingi250 – 2534GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-1799339. SRP-dependent cotranslational protein targeting to membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein 2
Short name:
SRP54
Gene namesi
Name:SRP-54B
Ordered Locus Names:At5g49500
ORF Names:K6M13.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G49500.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Signal recognition particle 54 kDa protein 2PRO_0000101205Add
BLAST

Proteomic databases

PaxDbiP49966.
PRIDEiP49966.

Expressioni

Gene expression databases

GenevisibleiP49966. AT.

Interactioni

Subunit structurei

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.

Protein-protein interaction databases

BioGridi20256. 2 interactions.
IntActiP49966. 1 interaction.
STRINGi3702.AT5G49500.1.

Structurei

3D structure databases

ProteinModelPortaliP49966.
SMRiP49966. Positions 1-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 297297G-domainAdd
BLAST
Regioni298 – 497200M-domainAdd
BLAST

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0780. Eukaryota.
COG0541. LUCA.
HOGENOMiHOG000036165.
InParanoidiP49966.
KOiK03106.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLAELGGRI MSAIQKMNNV TIIDEKALND CLNEITRALL QSDVSFPLVK
60 70 80 90 100
EMQTNIKKIV NLEDLAAGHN KRRIIEQAIF SELCKMLDPG KSAFAPKKAK
110 120 130 140 150
PSVVMFVGLQ GEVLEKPQLV PSMLIIIRRK GYKPALVCAD TFRAGAFDQL
160 170 180 190 200
KQNATKSKIP YYGSYTGSDP VKIAVEGVDR FKKENCDLII VDTSGRHKQQ
210 220 230 240 250
ASLFEEMRQI SEATKPDLVI FVMDSSIGQT AFEQARAFKQ TVAVGAVIIT
260 270 280 290 300
KMDGHAKGGG TLSAVAATKS PVIFIGTGEH MDEFEVFDAK PFVSRLLGNG
310 320 330 340 350
DMSGFVNKLQ EVVPKDQQPE LLEMLSHGKF TLRIMYDQFQ NMLNMGPLKE
360 370 380 390 400
VFSMLPGMRA EMMPEGHEKE SQAKIKRYMT MMDSMTNEEL DSSNPKVFNE
410 420 430 440 450
SRIMRIARGS GRIVREVMEM LEEYKRLTTM WGKVKGLKNL EKGNMSALSK
460 470 480 490
NKNSQQLSKV LPAQMLKQIG GMSGLQSLMK QMGSGKDLMG MFGGRDE
Length:497
Mass (Da):55,263
Last modified:July 27, 2011 - v2
Checksum:iA84627610B7B75A2
GO

Sequence cautioni

The sequence AAA66199.1 differs from that shown.Sequencing errors.Curated
The sequence AAA66199.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12126 Genomic DNA. Translation: AAA66199.1. Sequence problems.
AB023033 Genomic DNA. Translation: BAB10763.1.
CP002688 Genomic DNA. Translation: AED95821.1.
RefSeqiNP_199761.1. NM_124327.1.
UniGeneiAt.29781.

Genome annotation databases

EnsemblPlantsiAT5G49500.1; AT5G49500.1; AT5G49500.
GeneIDi835010.
GrameneiAT5G49500.1; AT5G49500.1; AT5G49500.
KEGGiath:AT5G49500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12126 Genomic DNA. Translation: AAA66199.1. Sequence problems.
AB023033 Genomic DNA. Translation: BAB10763.1.
CP002688 Genomic DNA. Translation: AED95821.1.
RefSeqiNP_199761.1. NM_124327.1.
UniGeneiAt.29781.

3D structure databases

ProteinModelPortaliP49966.
SMRiP49966. Positions 1-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20256. 2 interactions.
IntActiP49966. 1 interaction.
STRINGi3702.AT5G49500.1.

Proteomic databases

PaxDbiP49966.
PRIDEiP49966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G49500.1; AT5G49500.1; AT5G49500.
GeneIDi835010.
GrameneiAT5G49500.1; AT5G49500.1; AT5G49500.
KEGGiath:AT5G49500.

Organism-specific databases

TAIRiAT5G49500.

Phylogenomic databases

eggNOGiKOG0780. Eukaryota.
COG0541. LUCA.
HOGENOMiHOG000036165.
InParanoidiP49966.
KOiK03106.

Enzyme and pathway databases

ReactomeiR-ATH-1799339. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

PROiP49966.

Gene expression databases

GenevisibleiP49966. AT.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana expresses three divergent Srp54 genes."
    Chu B., Lindstrom J.T., Belanger F.C.
    Plant Physiol. 106:1157-1162(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiSR542_ARATH
AccessioniPrimary (citable) accession number: P49966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: February 17, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.