ID   PRP24_YEAST             Reviewed;         444 AA.
AC   P49960; D6W094; E9P966;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 174.
DE   RecName: Full=U4/U6 snRNA-associated-splicing factor PRP24;
DE            Short=U4/U6 snRNP protein;
GN   Name=PRP24; OrderedLocusNames=YMR268C; ORFNames=YM8156.10C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-288.
RX   PubMed=1827420; DOI=10.1101/gad.5.5.773;
RA   Shannon K.W., Guthrie C.;
RT   "Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with
RT   RNA-binding motifs.";
RL   Genes Dev. 5:773-785(1991).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=7882985; DOI=10.1002/j.1460-2075.1995.tb07060.x;
RA   Jandrositz A., Guthrie C.;
RT   "Evidence for a Prp24 binding site in U6 snRNA and in a putative
RT   intermediate in the annealing of U6 and U4 snRNAs.";
RL   EMBO J. 14:820-832(1995).
RN   [6]
RP   CHARACTERIZATION.
RX   PubMed=7585243;
RA   Ghetti A., Company M., Abelson J.;
RT   "Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic
RT   interaction of U4 and U6 snRNAs.";
RL   RNA 1:132-145(1995).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Binds preferentially to the U4/U6 hybrid snRNAs. Can
CC       stimulate the annealing of U4 and U6. Could participate in both the
CC       formation and disassembly of the U4/U6 hybrid during splicing.
CC   -!- INTERACTION:
CC       P49960; P47093: LSM8; NbExp=3; IntAct=EBI-212, EBI-313;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 672 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z49260; CAA89251.1; -; Genomic_DNA.
DR   EMBL; AY723858; AAU09775.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10168.1; -; Genomic_DNA.
DR   PIR; S54480; S54480.
DR   RefSeq; NP_013995.1; NM_001182775.1.
DR   PDB; 2GHP; X-ray; 2.70 A; A/B/C/D/E/F/G/H=1-291.
DR   PDB; 2GO9; NMR; -; A=38-197.
DR   PDB; 2KH9; NMR; -; A=115-197.
DR   PDB; 2L9W; NMR; -; A=292-400.
DR   PDB; 4N0T; X-ray; 1.70 A; A=34-400.
DR   PDB; 5TF6; X-ray; 2.30 A; A/C=34-400.
DR   PDB; 5VSU; X-ray; 3.10 A; A=1-444.
DR   PDB; 6ASO; X-ray; 2.71 A; A=28-444.
DR   PDBsum; 2GHP; -.
DR   PDBsum; 2GO9; -.
DR   PDBsum; 2KH9; -.
DR   PDBsum; 2L9W; -.
DR   PDBsum; 4N0T; -.
DR   PDBsum; 5TF6; -.
DR   PDBsum; 5VSU; -.
DR   PDBsum; 6ASO; -.
DR   AlphaFoldDB; P49960; -.
DR   BMRB; P49960; -.
DR   SMR; P49960; -.
DR   BioGRID; 35446; 574.
DR   ComplexPortal; CPX-24; U6 small nuclear ribonucleoprotein complex.
DR   DIP; DIP-2570N; -.
DR   IntAct; P49960; 9.
DR   MINT; P49960; -.
DR   STRING; 4932.YMR268C; -.
DR   iPTMnet; P49960; -.
DR   MaxQB; P49960; -.
DR   PaxDb; 4932-YMR268C; -.
DR   PeptideAtlas; P49960; -.
DR   EnsemblFungi; YMR268C_mRNA; YMR268C; YMR268C.
DR   GeneID; 855310; -.
DR   KEGG; sce:YMR268C; -.
DR   AGR; SGD:S000004881; -.
DR   SGD; S000004881; PRP24.
DR   VEuPathDB; FungiDB:YMR268C; -.
DR   eggNOG; KOG0128; Eukaryota.
DR   HOGENOM; CLU_621223_0_0_1; -.
DR   InParanoid; P49960; -.
DR   OMA; LPSLRYN; -.
DR   OrthoDB; 3035286at2759; -.
DR   BioCyc; YEAST:G3O-32941-MONOMER; -.
DR   BioGRID-ORCS; 855310; 0 hits in 10 CRISPR screens.
DR   EvolutionaryTrace; P49960; -.
DR   PRO; PR:P49960; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; P49960; Protein.
DR   GO; GO:0005634; C:nucleus; NAS:ComplexPortal.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; NAS:ComplexPortal.
DR   GO; GO:0005688; C:U6 snRNP; IDA:SGD.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0017069; F:snRNA binding; IPI:SGD.
DR   GO; GO:0017070; F:U6 snRNA binding; IDA:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; NAS:ComplexPortal.
DR   GO; GO:0000245; P:spliceosomal complex assembly; IMP:SGD.
DR   GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IDA:SGD.
DR   CDD; cd12296; RRM1_Prp24; 1.
DR   CDD; cd12297; RRM2_Prp24; 1.
DR   CDD; cd12298; RRM3_Prp24; 1.
DR   CDD; cd12299; RRM4_Prp24; 1.
DR   Gene3D; 3.30.70.330; -; 4.
DR   InterPro; IPR008669; LSM_interact.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR034397; Prp24_RRM1.
DR   InterPro; IPR034398; Prp24_RRM2.
DR   InterPro; IPR034540; Prp24_RRM3.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR031766; RRM_occluded.
DR   PANTHER; PTHR23003; RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN; 1.
DR   Pfam; PF05391; Lsm_interact; 1.
DR   Pfam; PF00076; RRM_1; 3.
DR   Pfam; PF16842; RRM_occluded; 1.
DR   SMART; SM00360; RRM; 4.
DR   SUPFAM; SSF54928; RNA-binding domain, RBD; 2.
DR   PROSITE; PS50102; RRM; 3.
PE   1: Evidence at protein level;
KW   3D-structure; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW   Reference proteome; Repeat; RNA-binding.
FT   CHAIN           1..444
FT                   /note="U4/U6 snRNA-associated-splicing factor PRP24"
FT                   /id="PRO_0000081736"
FT   DOMAIN          41..116
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          117..195
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          210..289
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         19
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
FT   CONFLICT        197
FT                   /note="E -> G (in Ref. 3; AAU09775)"
FT                   /evidence="ECO:0000305"
FT   HELIX           39..41
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          42..48
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           50..52
FT                   /evidence="ECO:0007829|PDB:2GO9"
FT   HELIX           54..61
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           62..64
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          67..74
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          78..89
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           90..97
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   TURN            98..101
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          110..113
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          118..122
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           130..139
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   TURN            140..142
FT                   /evidence="ECO:0007829|PDB:2KH9"
FT   STRAND          144..148
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           152..154
FT                   /evidence="ECO:0007829|PDB:2GHP"
FT   STRAND          160..166
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           168..178
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          189..192
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           195..197
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           204..207
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   TURN            208..210
FT                   /evidence="ECO:0007829|PDB:2GHP"
FT   STRAND          211..217
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           219..221
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           224..231
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           232..234
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          237..241
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   TURN            244..250
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          252..262
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           263..269
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           270..272
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          275..277
FT                   /evidence="ECO:0007829|PDB:5TF6"
FT   STRAND          283..286
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           290..302
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           307..310
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          313..318
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           325..334
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           340..342
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          343..349
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           350..352
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          354..361
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           362..372
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   STRAND          375..377
FT                   /evidence="ECO:0007829|PDB:5VSU"
FT   STRAND          383..385
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           387..397
FT                   /evidence="ECO:0007829|PDB:4N0T"
FT   HELIX           434..444
FT                   /evidence="ECO:0007829|PDB:5VSU"
SQ   SEQUENCE   444 AA;  50849 MW;  6BCCE7A9AAC51CE0 CRC64;
     MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK SYNQNKVYKY
     FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT HKVVGQNEII VSHLTECTLW
     MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP SLRFNTSRRF AYIDVTSKED ARYCVEKLNG
     LKIEGYTLVT KVSNPLEKSK RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI
     NIPAGQKEHS FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR
     LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS DFNGAIIIFR
     DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH SMKHVKPSCI NMMEKGPNLQ
     VKKKIPDKQE QMSNDDFRKM FLGE
//