Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P49960

- PRP24_YEAST

UniProt

P49960 - PRP24_YEAST

Protein

U4/U6 snRNA-associated-splicing factor PRP24

Gene

PRP24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. snRNA binding Source: SGD
    4. U6 snRNA binding Source: SGD

    GO - Biological processi

    1. spliceosomal complex assembly Source: SGD
    2. spliceosomal tri-snRNP complex assembly Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32941-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U4/U6 snRNA-associated-splicing factor PRP24
    Short name:
    U4/U6 snRNP protein
    Gene namesi
    Name:PRP24
    Ordered Locus Names:YMR268C
    ORF Names:YM8156.10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR268c.
    SGDiS000004881. PRP24.

    Subcellular locationi

    GO - Cellular componenti

    1. U6 snRNP Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444U4/U6 snRNA-associated-splicing factor PRP24PRO_0000081736Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP49960.
    PaxDbiP49960.
    PeptideAtlasiP49960.

    Expressioni

    Gene expression databases

    GenevestigatoriP49960.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LSM7P539053EBI-212,EBI-141
    LSM8P470933EBI-212,EBI-313

    Protein-protein interaction databases

    BioGridi35446. 36 interactions.
    DIPiDIP-2570N.
    IntActiP49960. 8 interactions.
    MINTiMINT-422430.
    STRINGi4932.YMR268C.

    Structurei

    Secondary structure

    1
    444
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi39 – 413
    Beta strandi42 – 487
    Helixi50 – 523
    Helixi54 – 618
    Helixi62 – 643
    Beta strandi67 – 748
    Beta strandi78 – 8912
    Helixi90 – 978
    Turni98 – 1014
    Beta strandi110 – 1134
    Beta strandi118 – 1225
    Helixi130 – 13910
    Turni140 – 1423
    Beta strandi144 – 1485
    Beta strandi152 – 1554
    Beta strandi160 – 1667
    Helixi168 – 17811
    Beta strandi189 – 1924
    Helixi195 – 1973
    Helixi204 – 2074
    Turni208 – 2103
    Beta strandi211 – 2177
    Helixi219 – 2213
    Helixi224 – 2318
    Helixi232 – 2343
    Beta strandi237 – 2415
    Turni244 – 2507
    Beta strandi252 – 26211
    Helixi263 – 2697
    Helixi270 – 2723
    Beta strandi275 – 2773
    Beta strandi283 – 2864
    Helixi290 – 30213
    Helixi307 – 3104
    Beta strandi313 – 3186
    Helixi325 – 33410
    Helixi340 – 3423
    Beta strandi343 – 3497
    Helixi350 – 3523
    Beta strandi354 – 3618
    Helixi362 – 37211
    Beta strandi373 – 3775
    Beta strandi383 – 3853
    Helixi387 – 39711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GHPX-ray2.70A/B/C/D/E/F/G/H1-291[»]
    2GO9NMR-A38-197[»]
    2KH9NMR-A115-197[»]
    2L9WNMR-A292-400[»]
    4N0TX-ray1.70A34-400[»]
    ProteinModelPortaliP49960.
    SMRiP49960. Positions 38-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49960.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 11676RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini117 – 19579RRM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini210 – 28980RRM 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG286439.
    HOGENOMiHOG000066054.
    OMAiWVTNFPP.
    OrthoDBiEOG706125.

    Family and domain databases

    Gene3Di3.30.70.330. 3 hits.
    InterProiIPR008669. LSM_interact.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF05391. Lsm_interact. 1 hit.
    PF00076. RRM_1. 3 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 3 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49960-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK    50
    SYNQNKVYKY FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT 100
    HKVVGQNEII VSHLTECTLW MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP 150
    SLRFNTSRRF AYIDVTSKED ARYCVEKLNG LKIEGYTLVT KVSNPLEKSK 200
    RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI NIPAGQKEHS 250
    FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR 300
    LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS 350
    DFNGAIIIFR DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH 400
    SMKHVKPSCI NMMEKGPNLQ VKKKIPDKQE QMSNDDFRKM FLGE 444
    Length:444
    Mass (Da):50,849
    Last modified:October 1, 1996 - v1
    Checksum:i6BCCE7A9AAC51CE0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti197 – 1971E → G in AAU09775. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49260 Genomic DNA. Translation: CAA89251.1.
    AY723858 Genomic DNA. Translation: AAU09775.1.
    BK006946 Genomic DNA. Translation: DAA10168.1.
    PIRiS54480.
    RefSeqiNP_013995.1. NM_001182775.1.

    Genome annotation databases

    EnsemblFungiiYMR268C; YMR268C; YMR268C.
    GeneIDi855310.
    KEGGisce:YMR268C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49260 Genomic DNA. Translation: CAA89251.1 .
    AY723858 Genomic DNA. Translation: AAU09775.1 .
    BK006946 Genomic DNA. Translation: DAA10168.1 .
    PIRi S54480.
    RefSeqi NP_013995.1. NM_001182775.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GHP X-ray 2.70 A/B/C/D/E/F/G/H 1-291 [» ]
    2GO9 NMR - A 38-197 [» ]
    2KH9 NMR - A 115-197 [» ]
    2L9W NMR - A 292-400 [» ]
    4N0T X-ray 1.70 A 34-400 [» ]
    ProteinModelPortali P49960.
    SMRi P49960. Positions 38-399.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35446. 36 interactions.
    DIPi DIP-2570N.
    IntActi P49960. 8 interactions.
    MINTi MINT-422430.
    STRINGi 4932.YMR268C.

    Proteomic databases

    MaxQBi P49960.
    PaxDbi P49960.
    PeptideAtlasi P49960.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR268C ; YMR268C ; YMR268C .
    GeneIDi 855310.
    KEGGi sce:YMR268C.

    Organism-specific databases

    CYGDi YMR268c.
    SGDi S000004881. PRP24.

    Phylogenomic databases

    eggNOGi NOG286439.
    HOGENOMi HOG000066054.
    OMAi WVTNFPP.
    OrthoDBi EOG706125.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32941-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P49960.
    NextBioi 978994.
    PROi P49960.

    Gene expression databases

    Genevestigatori P49960.

    Family and domain databases

    Gene3Di 3.30.70.330. 3 hits.
    InterProi IPR008669. LSM_interact.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF05391. Lsm_interact. 1 hit.
    PF00076. RRM_1. 3 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 3 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs."
      Shannon K.W., Guthrie C.
      Genes Dev. 5:773-785(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-288.
    5. "Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs."
      Jandrositz A., Guthrie C.
      EMBO J. 14:820-832(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs."
      Ghetti A., Company M., Abelson J.
      RNA 1:132-145(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRP24_YEAST
    AccessioniPrimary (citable) accession number: P49960
    Secondary accession number(s): D6W094, E9P966
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 672 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3