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P49960 (PRP24_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U4/U6 snRNA-associated-splicing factor PRP24

Short name=U4/U6 snRNP protein
Gene names
Name:PRP24
Ordered Locus Names:YMR268C
ORF Names:YM8156.10C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

Subcellular location

Nucleus.

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Sequence similarities

Contains 3 RRM (RNA recognition motif) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LSM7P539053EBI-212,EBI-141
LSM8P470933EBI-212,EBI-313

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444U4/U6 snRNA-associated-splicing factor PRP24
PRO_0000081736

Regions

Domain41 – 11676RRM 1
Domain117 – 19579RRM 2
Domain210 – 28980RRM 3

Amino acid modifications

Modified residue191Phosphoserine Ref.8 Ref.9

Experimental info

Sequence conflict1971E → G in AAU09775. Ref.3

Secondary structure

...................................................................... 444
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49960 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6BCCE7A9AAC51CE0

FASTA44450,849
        10         20         30         40         50         60 
MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK SYNQNKVYKY 

        70         80         90        100        110        120 
FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT HKVVGQNEII VSHLTECTLW 

       130        140        150        160        170        180 
MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP SLRFNTSRRF AYIDVTSKED ARYCVEKLNG 

       190        200        210        220        230        240 
LKIEGYTLVT KVSNPLEKSK RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI 

       250        260        270        280        290        300 
NIPAGQKEHS FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR 

       310        320        330        340        350        360 
LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS DFNGAIIIFR 

       370        380        390        400        410        420 
DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH SMKHVKPSCI NMMEKGPNLQ 

       430        440 
VKKKIPDKQE QMSNDDFRKM FLGE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs."
Shannon K.W., Guthrie C.
Genes Dev. 5:773-785(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-288.
[5]"Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs."
Jandrositz A., Guthrie C.
EMBO J. 14:820-832(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs."
Ghetti A., Company M., Abelson J.
RNA 1:132-145(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49260 Genomic DNA. Translation: CAA89251.1.
AY723858 Genomic DNA. Translation: AAU09775.1.
BK006946 Genomic DNA. Translation: DAA10168.1.
PIRS54480.
RefSeqNP_013995.1. NM_001182775.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2GHPX-ray2.70A/B/C/D/E/F/G/H1-291[»]
2GO9NMR-A38-197[»]
2KH9NMR-A115-197[»]
2L9WNMR-A292-400[»]
4N0TX-ray1.70A34-400[»]
ProteinModelPortalP49960.
SMRP49960. Positions 38-399.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35446. 36 interactions.
DIPDIP-2570N.
IntActP49960. 8 interactions.
MINTMINT-422430.
STRING4932.YMR268C.

Proteomic databases

MaxQBP49960.
PaxDbP49960.
PeptideAtlasP49960.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR268C; YMR268C; YMR268C.
GeneID855310.
KEGGsce:YMR268C.

Organism-specific databases

CYGDYMR268c.
SGDS000004881. PRP24.

Phylogenomic databases

eggNOGNOG286439.
HOGENOMHOG000066054.
OMAWVTNFPP.
OrthoDBEOG706125.

Enzyme and pathway databases

BioCycYEAST:G3O-32941-MONOMER.

Gene expression databases

GenevestigatorP49960.

Family and domain databases

Gene3D3.30.70.330. 3 hits.
InterProIPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTSM00360. RRM. 3 hits.
[Graphical view]
PROSITEPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP49960.
NextBio978994.
PROP49960.

Entry information

Entry namePRP24_YEAST
AccessionPrimary (citable) accession number: P49960
Secondary accession number(s): D6W094, E9P966
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references