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Protein

U4/U6 snRNA-associated-splicing factor PRP24

Gene

PRP24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • snRNA binding Source: SGD
  • U6 snRNA binding Source: SGD

GO - Biological processi

  • spliceosomal complex assembly Source: SGD
  • spliceosomal tri-snRNP complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32941-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 snRNA-associated-splicing factor PRP24
Short name:
U4/U6 snRNP protein
Gene namesi
Name:PRP24
Ordered Locus Names:YMR268C
ORF Names:YM8156.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR268C.
SGDiS000004881. PRP24.

Subcellular locationi

GO - Cellular componenti

  • U6 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817361 – 444U4/U6 snRNA-associated-splicing factor PRP24Add BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49960.
PRIDEiP49960.

PTM databases

iPTMnetiP49960.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM7P539053EBI-212,EBI-141
LSM8P470933EBI-212,EBI-313

Protein-protein interaction databases

BioGridi35446. 32 interactors.
DIPiDIP-2570N.
IntActiP49960. 9 interactors.
MINTiMINT-422430.

Structurei

Secondary structure

1444
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi39 – 41Combined sources3
Beta strandi42 – 48Combined sources7
Helixi50 – 52Combined sources3
Helixi54 – 61Combined sources8
Helixi62 – 64Combined sources3
Beta strandi67 – 74Combined sources8
Beta strandi78 – 89Combined sources12
Helixi90 – 97Combined sources8
Turni98 – 101Combined sources4
Beta strandi110 – 113Combined sources4
Beta strandi118 – 122Combined sources5
Helixi130 – 139Combined sources10
Turni140 – 142Combined sources3
Beta strandi144 – 148Combined sources5
Helixi152 – 154Combined sources3
Beta strandi160 – 166Combined sources7
Helixi168 – 178Combined sources11
Beta strandi189 – 192Combined sources4
Helixi195 – 197Combined sources3
Helixi204 – 207Combined sources4
Turni208 – 210Combined sources3
Beta strandi211 – 217Combined sources7
Helixi219 – 221Combined sources3
Helixi224 – 231Combined sources8
Helixi232 – 234Combined sources3
Beta strandi237 – 241Combined sources5
Turni244 – 250Combined sources7
Beta strandi252 – 262Combined sources11
Helixi263 – 269Combined sources7
Helixi270 – 272Combined sources3
Beta strandi275 – 277Combined sources3
Beta strandi283 – 286Combined sources4
Helixi290 – 302Combined sources13
Helixi307 – 310Combined sources4
Beta strandi313 – 318Combined sources6
Helixi325 – 334Combined sources10
Helixi340 – 342Combined sources3
Beta strandi343 – 349Combined sources7
Helixi350 – 352Combined sources3
Beta strandi354 – 361Combined sources8
Helixi362 – 372Combined sources11
Beta strandi373 – 377Combined sources5
Beta strandi383 – 385Combined sources3
Helixi387 – 397Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GHPX-ray2.70A/B/C/D/E/F/G/H1-291[»]
2GO9NMR-A38-197[»]
2KH9NMR-A115-197[»]
2L9WNMR-A292-400[»]
4N0TX-ray1.70A34-400[»]
ProteinModelPortaliP49960.
SMRiP49960.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49960.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 116RRM 1PROSITE-ProRule annotationAdd BLAST76
Domaini117 – 195RRM 2PROSITE-ProRule annotationAdd BLAST79
Domaini210 – 289RRM 3PROSITE-ProRule annotationAdd BLAST80

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000066054.
InParanoidiP49960.
OMAiTRNRELT.
OrthoDBiEOG092C5H62.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR031766. RRM_occluded.
[Graphical view]
PfamiPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
PF16842. RRM_occluded. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK
60 70 80 90 100
SYNQNKVYKY FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT
110 120 130 140 150
HKVVGQNEII VSHLTECTLW MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP
160 170 180 190 200
SLRFNTSRRF AYIDVTSKED ARYCVEKLNG LKIEGYTLVT KVSNPLEKSK
210 220 230 240 250
RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI NIPAGQKEHS
260 270 280 290 300
FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR
310 320 330 340 350
LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS
360 370 380 390 400
DFNGAIIIFR DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH
410 420 430 440
SMKHVKPSCI NMMEKGPNLQ VKKKIPDKQE QMSNDDFRKM FLGE
Length:444
Mass (Da):50,849
Last modified:October 1, 1996 - v1
Checksum:i6BCCE7A9AAC51CE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197E → G in AAU09775 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA. Translation: CAA89251.1.
AY723858 Genomic DNA. Translation: AAU09775.1.
BK006946 Genomic DNA. Translation: DAA10168.1.
PIRiS54480.
RefSeqiNP_013995.1. NM_001182775.1.

Genome annotation databases

EnsemblFungiiYMR268C; YMR268C; YMR268C.
GeneIDi855310.
KEGGisce:YMR268C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA. Translation: CAA89251.1.
AY723858 Genomic DNA. Translation: AAU09775.1.
BK006946 Genomic DNA. Translation: DAA10168.1.
PIRiS54480.
RefSeqiNP_013995.1. NM_001182775.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GHPX-ray2.70A/B/C/D/E/F/G/H1-291[»]
2GO9NMR-A38-197[»]
2KH9NMR-A115-197[»]
2L9WNMR-A292-400[»]
4N0TX-ray1.70A34-400[»]
ProteinModelPortaliP49960.
SMRiP49960.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35446. 32 interactors.
DIPiDIP-2570N.
IntActiP49960. 9 interactors.
MINTiMINT-422430.

PTM databases

iPTMnetiP49960.

Proteomic databases

MaxQBiP49960.
PRIDEiP49960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR268C; YMR268C; YMR268C.
GeneIDi855310.
KEGGisce:YMR268C.

Organism-specific databases

EuPathDBiFungiDB:YMR268C.
SGDiS000004881. PRP24.

Phylogenomic databases

HOGENOMiHOG000066054.
InParanoidiP49960.
OMAiTRNRELT.
OrthoDBiEOG092C5H62.

Enzyme and pathway databases

BioCyciYEAST:G3O-32941-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP49960.
PROiP49960.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR031766. RRM_occluded.
[Graphical view]
PfamiPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
PF16842. RRM_occluded. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP24_YEAST
AccessioniPrimary (citable) accession number: P49960
Secondary accession number(s): D6W094, E9P966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.