U4/U6 snRNA-associated-splicing factor PRP24 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P49960 (PRP24_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
U4/U6 snRNA-associated-splicing factor PRP24
Short name=U4/U6 snRNP protein

Gene names

Name:	PRP24
Ordered Locus Names:	YMR268C
ORF Names:	YM8156.10C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.
Subcellular location	Nucleus.
Miscellaneous	Present with 672 molecules/cell in log phase SD medium.
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	assembly of spliceosomal tri-snRNP Inferred from direct assay PubMed 9452384. Source: SGD spliceosomal complex assembly Inferred from mutant phenotype PubMed 10022888. Source: SGD
Cellular_component	U6 snRNP Inferred from direct assay PubMed 11720284. Source: SGD
Molecular_function	U6 snRNA binding Inferred from direct assay PubMed 20181740. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 444

444

U4/U6 snRNA-associated-splicing factor PRP24

PRO_0000081736

Regions

Domain

41 – 116

RRM 1

Domain

117 – 195

RRM 2

Domain

210 – 289

RRM 3

Amino acid modifications

Modified residue

Phosphoserine Ref.8 Ref.9

Experimental info

Sequence conflict

197

E → G in AAU09775. Ref.3

Secondary structure

444

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P49960 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6BCCE7A9AAC51CE0
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FASTA

444

50,849

        10         20         30         40         50         60 
MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK SYNQNKVYKY 

        70         80         90        100        110        120 
FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT HKVVGQNEII VSHLTECTLW 

       130        140        150        160        170        180 
MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP SLRFNTSRRF AYIDVTSKED ARYCVEKLNG 

       190        200        210        220        230        240 
LKIEGYTLVT KVSNPLEKSK RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI 

       250        260        270        280        290        300 
NIPAGQKEHS FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR 

       310        320        330        340        350        360 
LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS DFNGAIIIFR 

       370        380        390        400        410        420 
DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH SMKHVKPSCI NMMEKGPNLQ 

       430        440 
VKKKIPDKQE QMSNDDFRKM FLGE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs." Shannon K.W., Guthrie C. Genes Dev. 5:773-785(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-288.
[5]	"Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs." Jandrositz A., Guthrie C. EMBO J. 14:820-832(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs." Ghetti A., Company M., Abelson J. RNA 1:132-145(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49260 Genomic DNA. Translation: CAA89251.1.
AY723858 Genomic DNA. Translation: AAU09775.1.
BK006946 Genomic DNA. Translation: DAA10168.1.

PIR

S54480.

RefSeq

NP_013995.1. NM_001182775.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GHP	X-ray	2.70	A/B/C/D/E/F/G/H	1-291	[»]
2GO9	NMR	-	A	38-197	[»]
2KH9	NMR	-	A	115-197	[»]
2L9W	NMR	-	A	292-400	[»]

ProteinModelPortal

P49960.

SMR

P49960. Positions 38-399.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2570N.

IntAct

P49960. 6 interactions.

MINT

MINT-422430.

STRING

4932.YMR268C.

Proteomic databases

PaxDb

P49960.

PeptideAtlas

P49960.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YMR268C; YMR268C; YMR268C.

GeneID

855310.

KEGG

sce:YMR268C.

Organism-specific databases

CYGD

YMR268c.

SGD

S000004881. PRP24.

Phylogenomic databases

eggNOG

NOG286439.

HOGENOM

HOG000066054.

OMA

LLVSDHE.

OrthoDB

EOG486CNQ.

Gene expression databases

Genevestigator

P49960.

GermOnline

YMR268C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.70.330. 3 hits.

InterPro

IPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]

SMART

SM00360. RRM. 3 hits.
[Graphical view]

PROSITE

PS50102. RRM. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P49960.

NextBio

978994.

Entry information

Entry name

PRP24_YEAST

Accession

Primary (citable) accession number: P49960
Secondary accession number(s): D6W094, E9P966

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents