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P49960

- PRP24_YEAST

UniProt

P49960 - PRP24_YEAST

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Protein
U4/U6 snRNA-associated-splicing factor PRP24
Gene
PRP24, YMR268C, YM8156.10C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

GO - Molecular functioni

  1. U6 snRNA binding Source: SGD
  2. nucleotide binding Source: InterPro
  3. protein binding Source: IntAct
  4. snRNA binding Source: SGD

GO - Biological processi

  1. spliceosomal complex assembly Source: SGD
  2. spliceosomal tri-snRNP complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32941-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 snRNA-associated-splicing factor PRP24
Short name:
U4/U6 snRNP protein
Gene namesi
Name:PRP24
Ordered Locus Names:YMR268C
ORF Names:YM8156.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR268c.
SGDiS000004881. PRP24.

Subcellular locationi

GO - Cellular componenti

  1. U6 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444U4/U6 snRNA-associated-splicing factor PRP24
PRO_0000081736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49960.
PaxDbiP49960.
PeptideAtlasiP49960.

Expressioni

Gene expression databases

GenevestigatoriP49960.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM7P539053EBI-212,EBI-141
LSM8P470933EBI-212,EBI-313

Protein-protein interaction databases

BioGridi35446. 36 interactions.
DIPiDIP-2570N.
IntActiP49960. 8 interactions.
MINTiMINT-422430.
STRINGi4932.YMR268C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 413
Beta strandi42 – 487
Helixi50 – 523
Helixi54 – 618
Helixi62 – 643
Beta strandi67 – 748
Beta strandi78 – 8912
Helixi90 – 978
Turni98 – 1014
Beta strandi110 – 1134
Beta strandi118 – 1225
Helixi130 – 13910
Turni140 – 1423
Beta strandi144 – 1485
Beta strandi152 – 1554
Beta strandi160 – 1667
Helixi168 – 17811
Beta strandi189 – 1924
Helixi195 – 1973
Helixi204 – 2074
Turni208 – 2103
Beta strandi211 – 2177
Helixi219 – 2213
Helixi224 – 2318
Helixi232 – 2343
Beta strandi237 – 2415
Turni244 – 2507
Beta strandi252 – 26211
Helixi263 – 2697
Helixi270 – 2723
Beta strandi275 – 2773
Beta strandi283 – 2864
Helixi290 – 30213
Helixi307 – 3104
Beta strandi313 – 3186
Helixi325 – 33410
Helixi340 – 3423
Beta strandi343 – 3497
Helixi350 – 3523
Beta strandi354 – 3618
Helixi362 – 37211
Beta strandi373 – 3775
Beta strandi383 – 3853
Helixi387 – 39711

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GHPX-ray2.70A/B/C/D/E/F/G/H1-291[»]
2GO9NMR-A38-197[»]
2KH9NMR-A115-197[»]
2L9WNMR-A292-400[»]
4N0TX-ray1.70A34-400[»]
ProteinModelPortaliP49960.
SMRiP49960. Positions 38-399.

Miscellaneous databases

EvolutionaryTraceiP49960.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 11676RRM 1
Add
BLAST
Domaini117 – 19579RRM 2
Add
BLAST
Domaini210 – 28980RRM 3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG286439.
HOGENOMiHOG000066054.
OMAiWVTNFPP.
OrthoDBiEOG706125.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49960-1 [UniParc]FASTAAdd to Basket

« Hide

MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK    50
SYNQNKVYKY FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT 100
HKVVGQNEII VSHLTECTLW MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP 150
SLRFNTSRRF AYIDVTSKED ARYCVEKLNG LKIEGYTLVT KVSNPLEKSK 200
RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI NIPAGQKEHS 250
FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR 300
LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS 350
DFNGAIIIFR DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH 400
SMKHVKPSCI NMMEKGPNLQ VKKKIPDKQE QMSNDDFRKM FLGE 444
Length:444
Mass (Da):50,849
Last modified:October 1, 1996 - v1
Checksum:i6BCCE7A9AAC51CE0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971E → G in AAU09775. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49260 Genomic DNA. Translation: CAA89251.1.
AY723858 Genomic DNA. Translation: AAU09775.1.
BK006946 Genomic DNA. Translation: DAA10168.1.
PIRiS54480.
RefSeqiNP_013995.1. NM_001182775.1.

Genome annotation databases

EnsemblFungiiYMR268C; YMR268C; YMR268C.
GeneIDi855310.
KEGGisce:YMR268C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49260 Genomic DNA. Translation: CAA89251.1 .
AY723858 Genomic DNA. Translation: AAU09775.1 .
BK006946 Genomic DNA. Translation: DAA10168.1 .
PIRi S54480.
RefSeqi NP_013995.1. NM_001182775.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GHP X-ray 2.70 A/B/C/D/E/F/G/H 1-291 [» ]
2GO9 NMR - A 38-197 [» ]
2KH9 NMR - A 115-197 [» ]
2L9W NMR - A 292-400 [» ]
4N0T X-ray 1.70 A 34-400 [» ]
ProteinModelPortali P49960.
SMRi P49960. Positions 38-399.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35446. 36 interactions.
DIPi DIP-2570N.
IntActi P49960. 8 interactions.
MINTi MINT-422430.
STRINGi 4932.YMR268C.

Proteomic databases

MaxQBi P49960.
PaxDbi P49960.
PeptideAtlasi P49960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR268C ; YMR268C ; YMR268C .
GeneIDi 855310.
KEGGi sce:YMR268C.

Organism-specific databases

CYGDi YMR268c.
SGDi S000004881. PRP24.

Phylogenomic databases

eggNOGi NOG286439.
HOGENOMi HOG000066054.
OMAi WVTNFPP.
OrthoDBi EOG706125.

Enzyme and pathway databases

BioCyci YEAST:G3O-32941-MONOMER.

Miscellaneous databases

EvolutionaryTracei P49960.
NextBioi 978994.
PROi P49960.

Gene expression databases

Genevestigatori P49960.

Family and domain databases

Gene3Di 3.30.70.330. 3 hits.
InterProi IPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view ]
SMARTi SM00360. RRM. 3 hits.
[Graphical view ]
PROSITEi PS50102. RRM. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs."
    Shannon K.W., Guthrie C.
    Genes Dev. 5:773-785(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-288.
  5. "Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs."
    Jandrositz A., Guthrie C.
    EMBO J. 14:820-832(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs."
    Ghetti A., Company M., Abelson J.
    RNA 1:132-145(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP24_YEAST
AccessioniPrimary (citable) accession number: P49960
Secondary accession number(s): D6W094, E9P966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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