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P49960

- PRP24_YEAST

UniProt

P49960 - PRP24_YEAST

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Protein

U4/U6 snRNA-associated-splicing factor PRP24

Gene

PRP24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. snRNA binding Source: SGD
  3. U6 snRNA binding Source: SGD

GO - Biological processi

  1. spliceosomal complex assembly Source: SGD
  2. spliceosomal tri-snRNP complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32941-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 snRNA-associated-splicing factor PRP24
Short name:
U4/U6 snRNP protein
Gene namesi
Name:PRP24
Ordered Locus Names:YMR268C
ORF Names:YM8156.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR268c.
SGDiS000004881. PRP24.

Subcellular locationi

GO - Cellular componenti

  1. U6 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444U4/U6 snRNA-associated-splicing factor PRP24PRO_0000081736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49960.
PaxDbiP49960.
PeptideAtlasiP49960.

Expressioni

Gene expression databases

GenevestigatoriP49960.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM7P539053EBI-212,EBI-141
LSM8P470933EBI-212,EBI-313

Protein-protein interaction databases

BioGridi35446. 36 interactions.
DIPiDIP-2570N.
IntActiP49960. 8 interactions.
MINTiMINT-422430.
STRINGi4932.YMR268C.

Structurei

Secondary structure

1
444
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 413Combined sources
Beta strandi42 – 487Combined sources
Helixi50 – 523Combined sources
Helixi54 – 618Combined sources
Helixi62 – 643Combined sources
Beta strandi67 – 748Combined sources
Beta strandi78 – 8912Combined sources
Helixi90 – 978Combined sources
Turni98 – 1014Combined sources
Beta strandi110 – 1134Combined sources
Beta strandi118 – 1225Combined sources
Helixi130 – 13910Combined sources
Turni140 – 1423Combined sources
Beta strandi144 – 1485Combined sources
Beta strandi152 – 1554Combined sources
Beta strandi160 – 1667Combined sources
Helixi168 – 17811Combined sources
Beta strandi189 – 1924Combined sources
Helixi195 – 1973Combined sources
Helixi204 – 2074Combined sources
Turni208 – 2103Combined sources
Beta strandi211 – 2177Combined sources
Helixi219 – 2213Combined sources
Helixi224 – 2318Combined sources
Helixi232 – 2343Combined sources
Beta strandi237 – 2415Combined sources
Turni244 – 2507Combined sources
Beta strandi252 – 26211Combined sources
Helixi263 – 2697Combined sources
Helixi270 – 2723Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi283 – 2864Combined sources
Helixi290 – 30213Combined sources
Helixi307 – 3104Combined sources
Beta strandi313 – 3186Combined sources
Helixi325 – 33410Combined sources
Helixi340 – 3423Combined sources
Beta strandi343 – 3497Combined sources
Helixi350 – 3523Combined sources
Beta strandi354 – 3618Combined sources
Helixi362 – 37211Combined sources
Beta strandi373 – 3775Combined sources
Beta strandi383 – 3853Combined sources
Helixi387 – 39711Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GHPX-ray2.70A/B/C/D/E/F/G/H1-291[»]
2GO9NMR-A38-197[»]
2KH9NMR-A115-197[»]
2L9WNMR-A292-400[»]
4N0TX-ray1.70A34-400[»]
ProteinModelPortaliP49960.
SMRiP49960. Positions 36-398.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49960.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 11676RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini117 – 19579RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini210 – 28980RRM 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG286439.
HOGENOMiHOG000066054.
InParanoidiP49960.
OMAiWVTNFPP.
OrthoDBiEOG706125.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49960-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEYGHHARPD SKRPLDEGSP AAAGLTSKKA NEALTRNREL TTVLVKNLPK
60 70 80 90 100
SYNQNKVYKY FKHCGPIIHV DVADSLKKNF RFARIEFARY DGALAAITKT
110 120 130 140 150
HKVVGQNEII VSHLTECTLW MTNFPPSYTQ RNIRDLLQDI NVVALSIRLP
160 170 180 190 200
SLRFNTSRRF AYIDVTSKED ARYCVEKLNG LKIEGYTLVT KVSNPLEKSK
210 220 230 240 250
RTDSATLEGR EIMIRNLSTE LLDENLLRES FEGFGSIEKI NIPAGQKEHS
260 270 280 290 300
FNNCCAFMVF ENKDSAERAL QMNRSLLGNR EISVSLADKK PFLERNEVKR
310 320 330 340 350
LLASRNSKEL ETLICLFPLS DKVSPSLICQ FLQEEIHINE KDIRKILLVS
360 370 380 390 400
DFNGAIIIFR DSKFAAKMLM ILNGSQFQGK VIRSGTINDM KRYYNNQQNH
410 420 430 440
SMKHVKPSCI NMMEKGPNLQ VKKKIPDKQE QMSNDDFRKM FLGE
Length:444
Mass (Da):50,849
Last modified:October 1, 1996 - v1
Checksum:i6BCCE7A9AAC51CE0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971E → G in AAU09775. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49260 Genomic DNA. Translation: CAA89251.1.
AY723858 Genomic DNA. Translation: AAU09775.1.
BK006946 Genomic DNA. Translation: DAA10168.1.
PIRiS54480.
RefSeqiNP_013995.1. NM_001182775.1.

Genome annotation databases

EnsemblFungiiYMR268C; YMR268C; YMR268C.
GeneIDi855310.
KEGGisce:YMR268C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49260 Genomic DNA. Translation: CAA89251.1 .
AY723858 Genomic DNA. Translation: AAU09775.1 .
BK006946 Genomic DNA. Translation: DAA10168.1 .
PIRi S54480.
RefSeqi NP_013995.1. NM_001182775.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GHP X-ray 2.70 A/B/C/D/E/F/G/H 1-291 [» ]
2GO9 NMR - A 38-197 [» ]
2KH9 NMR - A 115-197 [» ]
2L9W NMR - A 292-400 [» ]
4N0T X-ray 1.70 A 34-400 [» ]
ProteinModelPortali P49960.
SMRi P49960. Positions 36-398.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35446. 36 interactions.
DIPi DIP-2570N.
IntActi P49960. 8 interactions.
MINTi MINT-422430.
STRINGi 4932.YMR268C.

Proteomic databases

MaxQBi P49960.
PaxDbi P49960.
PeptideAtlasi P49960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR268C ; YMR268C ; YMR268C .
GeneIDi 855310.
KEGGi sce:YMR268C.

Organism-specific databases

CYGDi YMR268c.
SGDi S000004881. PRP24.

Phylogenomic databases

eggNOGi NOG286439.
HOGENOMi HOG000066054.
InParanoidi P49960.
OMAi WVTNFPP.
OrthoDBi EOG706125.

Enzyme and pathway databases

BioCyci YEAST:G3O-32941-MONOMER.

Miscellaneous databases

EvolutionaryTracei P49960.
NextBioi 978994.
PROi P49960.

Gene expression databases

Genevestigatori P49960.

Family and domain databases

Gene3Di 3.30.70.330. 3 hits.
InterProi IPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view ]
SMARTi SM00360. RRM. 3 hits.
[Graphical view ]
PROSITEi PS50102. RRM. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs."
    Shannon K.W., Guthrie C.
    Genes Dev. 5:773-785(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-288.
  5. "Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs."
    Jandrositz A., Guthrie C.
    EMBO J. 14:820-832(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs."
    Ghetti A., Company M., Abelson J.
    RNA 1:132-145(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP24_YEAST
AccessioniPrimary (citable) accession number: P49960
Secondary accession number(s): D6W094, E9P966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3