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Protein

tRNA (carboxymethyluridine(34)-5-O)-methyltransferase

Gene

TRM9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the methylation of the wobble bases at position 34 in tRNA. Appears to have a role in stress-response.1 Publication

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + carboxymethyluridine(34) in tRNA = S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to drug Source: SGD
  • chronological cell aging Source: SGD
  • regulation of translational elongation Source: SGD
  • tRNA methylation Source: SGD
  • tRNA modification Source: Reactome
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32618-MONOMER
YEAST:G3O-32618-MONOMER
BRENDAi2.1.1.229 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase (EC:2.1.1.229)
Alternative name(s):
tRNA (uracil-5-)-methyltransferase TRM9
tRNA [Um34] methyltransferase
tRNA methylase 9
Gene namesi
Name:TRM9
Ordered Locus Names:YML014W
ORF Names:YM9571.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML014W
SGDiS000004476 TRM9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002032621 – 279tRNA (carboxymethyluridine(34)-5-O)-methyltransferaseAdd BLAST279

Proteomic databases

MaxQBiP49957
PaxDbiP49957
PRIDEiP49957

PTM databases

iPTMnetiP49957

Interactioni

Subunit structurei

Interacts with TRM112.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TRM112P537383EBI-27735,EBI-28520

Protein-protein interaction databases

BioGridi35155, 109 interactors
DIPiDIP-4549N
IntActiP49957, 5 interactors
MINTiP49957
STRINGi4932.YML014W

Structurei

3D structure databases

ProteinModelPortaliP49957
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00530000063536
HOGENOMiHOG000209191
InParanoidiP49957
KOiK15444
OMAiTREYAWP
OrthoDBiEOG092C4H6Z

Family and domain databases

InterProiView protein in InterPro
IPR013216 Methyltransf_11
IPR029063 SAM-dependent_MTases
IPR032873 Trm9
PANTHERiPTHR13069:SF21 PTHR13069:SF21, 1 hit
PfamiView protein in Pfam
PF08241 Methyltransf_11, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P49957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEINQAAEKE QEYVHKVYNE IAPHFSQTRY KPWPIVTQFL KTRPMGSIGI
60 70 80 90 100
DVGCGNGKYL GVNPDIYIIG SDRSDGLIEC ARGINPSYNL LVADGLNLPH
110 120 130 140 150
KNETFDFAIS IAVVHHWSTR ERRVEVIRHV LSKLRQGGQA LIYCWALEQG
160 170 180 190 200
SSRRGYHEGM EQDVFVPWVL PKSKSKPKTK STPPAKVKTR PKPNLMNIPP
210 220 230 240 250
KERSEYLQRW KEEQQRSKSL DDNDEKQQQD QEQEREEVKY RYYHLYREGE
260 270
LAEDCRQAGA AVHSEGFERD NWWVVAQKR
Length:279
Mass (Da):32,439
Last modified:October 1, 1996 - v1
Checksum:i2F8795BB3C640D3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA Translation: CAA89938.1
BK006946 Genomic DNA Translation: DAA09884.1
PIRiS55105
RefSeqiNP_013698.1, NM_001182372.1

Genome annotation databases

EnsemblFungiiYML014W; YML014W; YML014W
GeneIDi854994
KEGGisce:YML014W

Similar proteinsi

Entry informationi

Entry nameiTRM9_YEAST
AccessioniPrimary (citable) accession number: P49957
Secondary accession number(s): D6VZG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health