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Protein

tRNA (carboxymethyluridine(34)-5-O)-methyltransferase

Gene

TRM9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the methylation of the wobble bases at position 34 in tRNA. Appears to have a role in stress-response.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + carboxymethyluridine(34) in tRNA = S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to drug Source: SGD
  • chronological cell aging Source: SGD
  • tRNA methylation Source: SGD
  • tRNA modification Source: Reactome
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16700.
YEAST:G3O-32618-MONOMER.
BRENDAi2.1.1.229. 984.
ReactomeiR-SCE-6782315. tRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase (EC:2.1.1.229)
Alternative name(s):
tRNA (uracil-5-)-methyltransferase TRM9
tRNA [Um34] methyltransferase
tRNA methylase 9
Gene namesi
Name:TRM9
Ordered Locus Names:YML014W
ORF Names:YM9571.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML014W.
SGDiS000004476. TRM9.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279tRNA (carboxymethyluridine(34)-5-O)-methyltransferasePRO_0000203262Add
BLAST

Proteomic databases

MaxQBiP49957.

PTM databases

iPTMnetiP49957.

Interactioni

Subunit structurei

Interacts with TRM112.1 Publication

Protein-protein interaction databases

BioGridi35155. 67 interactions.
DIPiDIP-4549N.
IntActiP49957. 3 interactions.
MINTiMINT-561829.

Structurei

3D structure databases

ProteinModelPortaliP49957.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00530000063536.
HOGENOMiHOG000209191.
InParanoidiP49957.
KOiK15444.
OMAiEGMEQDV.
OrthoDBiEOG7J70SV.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P49957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEINQAAEKE QEYVHKVYNE IAPHFSQTRY KPWPIVTQFL KTRPMGSIGI
60 70 80 90 100
DVGCGNGKYL GVNPDIYIIG SDRSDGLIEC ARGINPSYNL LVADGLNLPH
110 120 130 140 150
KNETFDFAIS IAVVHHWSTR ERRVEVIRHV LSKLRQGGQA LIYCWALEQG
160 170 180 190 200
SSRRGYHEGM EQDVFVPWVL PKSKSKPKTK STPPAKVKTR PKPNLMNIPP
210 220 230 240 250
KERSEYLQRW KEEQQRSKSL DDNDEKQQQD QEQEREEVKY RYYHLYREGE
260 270
LAEDCRQAGA AVHSEGFERD NWWVVAQKR
Length:279
Mass (Da):32,439
Last modified:October 1, 1996 - v1
Checksum:i2F8795BB3C640D3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89938.1.
BK006946 Genomic DNA. Translation: DAA09884.1.
PIRiS55105.
RefSeqiNP_013698.1. NM_001182372.1.

Genome annotation databases

EnsemblFungiiYML014W; YML014W; YML014W.
GeneIDi854994.
KEGGisce:YML014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89938.1.
BK006946 Genomic DNA. Translation: DAA09884.1.
PIRiS55105.
RefSeqiNP_013698.1. NM_001182372.1.

3D structure databases

ProteinModelPortaliP49957.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35155. 67 interactions.
DIPiDIP-4549N.
IntActiP49957. 3 interactions.
MINTiMINT-561829.

PTM databases

iPTMnetiP49957.

Proteomic databases

MaxQBiP49957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML014W; YML014W; YML014W.
GeneIDi854994.
KEGGisce:YML014W.

Organism-specific databases

EuPathDBiFungiDB:YML014W.
SGDiS000004476. TRM9.

Phylogenomic databases

GeneTreeiENSGT00530000063536.
HOGENOMiHOG000209191.
InParanoidiP49957.
KOiK15444.
OMAiEGMEQDV.
OrthoDBiEOG7J70SV.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16700.
YEAST:G3O-32618-MONOMER.
BRENDAi2.1.1.229. 984.
ReactomeiR-SCE-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

PROiP49957.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Novel methyltransferase for modified uridine residues at the wobble position of tRNA."
    Kalhor H.R., Clarke S.
    Mol. Cell. Biol. 23:9283-9292(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA."
    Purushothaman S.K., Bujnicki J.M., Grosjean H., Lapeyre B.
    Mol. Cell. Biol. 25:4359-4370(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRM112.

Entry informationi

Entry nameiTRM9_YEAST
AccessioniPrimary (citable) accession number: P49957
Secondary accession number(s): D6VZG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.