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Protein

Chromosome transmission fidelity protein 18

Gene

CTF18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 190ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA replication initiation Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • maintenance of DNA trinucleotide repeats Source: SGD
  • mitotic sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 18
Gene namesi
Name:CTF18
Synonyms:CHL12
Ordered Locus Names:YMR078C
ORF Names:YM9582.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR078C.
SGDiS000004683. CTF18.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189K → E: Fails to unload PCNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896451 – 741Chromosome transmission fidelity protein 18Add BLAST741

Proteomic databases

MaxQBiP49956.
PRIDEiP49956.

PTM databases

iPTMnetiP49956.

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. CTF18 interacts with ECO1.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35254. 508 interactors.
DIPiDIP-5868N.
IntActiP49956. 15 interactors.
MINTiMINT-626774.
STRINGi4932.YMR078C.

Structurei

Secondary structure

1741
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi719 – 722Combined sources4
Helixi736 – 739Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MSMX-ray2.29C/F666-741[»]
ProteinModelPortaliP49956.
SMRiP49956.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075029.
HOGENOMiHOG000066044.
InParanoidiP49956.
KOiK11269.
OMAiCNDVYHP.
OrthoDBiEOG092C2PM6.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P49956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDTAPYIGS LGRSSLFDTG DIEQAPGNNA IGINEEDIHA FVSSTGETVQ
60 70 80 90 100
LKKKPAKLAT GNISLYTNPD TVWRSDDTYG ININYLLDKI EASGDDRTNA
110 120 130 140 150
QKTSPITGKI GSDTLWVEKW RPKKFLDLVG NEKTNRRMLG WLRQWTPAVF
160 170 180 190 200
KEQLPKLPTE KEVSDMELDP LKRPPKKILL LHGPPGIGKT SVAHVIAKQS
210 220 230 240 250
GFSVSEINAS DERAGPMVKE KIYNLLFNHT FDTNPVCLVA DEIDGSIESG
260 270 280 290 300
FIRILVDIMQ SDIKATNKLL YGQPDKKDKK RKKKRSKLLT RPIICICNNL
310 320 330 340 350
YAPSLEKLKP FCEIIAVKRP SDTTLLERLN LICHKENMNI PIKAINDLID
360 370 380 390 400
LAQGDVRNCI NNLQFLASNV DSRDSSASDK PACAKNTWAS SNKDSPISWF
410 420 430 440 450
KIVNQLFRKD PHRDIKEQFY ELLNQVELNG NSDRILQGCF NIFPYVKYSD
460 470 480 490 500
NGIRKPANIS DWLFFHDLMY QSMYAHNGEL LRYSALVPLV FFQTFGDIAN
510 520 530 540 550
KDDIRMKNSE YEQRELKRAN SDIVSLIMRH ISVQSPLMAS FTDRKSLIFE
560 570 580 590 600
ILPYLDSMIS SDFNKIRNLK LKQAIMEELV QLLKSFQLNL IQNRSEGFDV
610 620 630 640 650
RGGLTIDPPI DEVVLLNPKH INEVQHKRAN NLSSLLAKIE ENRAKKRHID
660 670 680 690 700
QVTEDRLQSQ EMHSKKVKTG LNSSSSTIDF FKNQYGLLKQ TQELEETQKT
710 720 730 740
IGSDETNQAD DCNQTVKIWV KYNEGFSNAV RKNVTWNNLW E
Length:741
Mass (Da):84,373
Last modified:October 1, 1996 - v1
Checksum:iDC054EA712BD1BB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24514 Genomic DNA. No translation available.
Z49259 Genomic DNA. Translation: CAA89224.1.
BK006946 Genomic DNA. Translation: DAA09976.1.
PIRiS50340.
RefSeqiNP_013795.1. NM_001182577.1.

Genome annotation databases

EnsemblFungiiYMR078C; YMR078C; YMR078C.
GeneIDi855102.
KEGGisce:YMR078C.

Similar proteinsi

Entry informationi

Entry nameiCTF18_YEAST
AccessioniPrimary (citable) accession number: P49956
Secondary accession number(s): D6VZQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names