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P49955

- SF3B1_YEAST

UniProt

P49955 - SF3B1_YEAST

Protein

U2 snRNP component HSH155

Gene

HSH155

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Contacts pre-mRNA on both sides of the branch site early in spliceosome assembly.By similarity

    GO - Molecular functioni

    1. mRNA binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD
    2. spliceosomal complex assembly Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32958-MONOMER.
    ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U2 snRNP component HSH155
    Gene namesi
    Name:HSH155
    Ordered Locus Names:YMR288W
    ORF Names:YM8021.14
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR288w.
    SGDiS000004901. HSH155.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. U2 snRNP Source: SGD
    2. U2-type prespliceosome Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 971971U2 snRNP component HSH155PRO_0000174327Add
    BLAST

    Proteomic databases

    MaxQBiP49955.
    PaxDbiP49955.
    PeptideAtlasiP49955.

    Expressioni

    Gene expression databases

    GenevestigatoriP49955.

    Interactioni

    Subunit structurei

    Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSE1Q046935EBI-664,EBI-519
    YSF3P0C0743EBI-664,EBI-970846

    Protein-protein interaction databases

    BioGridi35468. 60 interactions.
    DIPiDIP-2628N.
    IntActiP49955. 27 interactions.
    MINTiMINT-426520.
    STRINGi4932.YMR288W.

    Structurei

    3D structure databases

    ProteinModelPortaliP49955.
    SMRiP49955. Positions 579-610, 647-711.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati199 – 23739HEAT 1Add
    BLAST
    Repeati273 – 31038HEAT 2Add
    BLAST
    Repeati350 – 38738HEAT 3Add
    BLAST
    Repeati513 – 55038HEAT 4Add
    BLAST
    Repeati596 – 63338HEAT 5Add
    BLAST
    Repeati680 – 71738HEAT 6Add
    BLAST
    Repeati722 – 75938HEAT 7Add
    BLAST
    Repeati792 – 82938HEAT 8Add
    BLAST
    Repeati832 – 87039HEAT 9Add
    BLAST

    Sequence similaritiesi

    Belongs to the SF3B1 family.Curated
    Contains 9 HEAT repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5181.
    GeneTreeiENSGT00390000018393.
    HOGENOMiHOG000166737.
    KOiK12828.
    OMAiAFWVRRM.
    OrthoDBiEOG754HXV.

    Family and domain databases

    Gene3Di1.25.10.10. 3 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P49955-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE    50
    KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR 100
    WDVKGYEPPD ESSTAVKENS DSALVNVEGI HDLMFFKPSD HKYFADVISK 150
    KPIDELNKDE KKERTLSMLL LKIKNGNTAS RRTSMRILTD KAVTFGPEMI 200
    FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV HKILVVAAPL 250
    LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA 300
    AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN 350
    HLTGLMSCIK DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL 400
    WKGIRSHRGK VLSSFLKAVG SMIPLMDPEY AGYYTTEAMR IIRREFDSPD 450
    DEMKKTILLV LQKCSAVESI TPKFLREEIA PEFFQKFWVR RVALDRPLNK 500
    VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA VTRTVNLLGT 550
    ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP 600
    IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES 650
    LGEVYPEVLG SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV 700
    EVNTIKFVGL IGKLAPTYAP PKEWMRICFE LLELLKSTNK EIRRSANATF 750
    GFIAEAIGPH DVLVALLNNL KVQERQLRVC TAVAIGIVAK VCGPYNVLPV 800
    IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD 850
    LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL 900
    EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV 950
    PFYPVTPDNN EEYIEELDLV L 971
    Length:971
    Mass (Da):110,028
    Last modified:October 1, 1996 - v1
    Checksum:i27D26E4252A788E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49704 Genomic DNA. Translation: CAA89786.1.
    BK006946 Genomic DNA. Translation: DAA10189.1.
    PIRiS54595.
    RefSeqiNP_014015.1. NM_001182795.1.

    Genome annotation databases

    EnsemblFungiiYMR288W; YMR288W; YMR288W.
    GeneIDi855332.
    KEGGisce:YMR288W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49704 Genomic DNA. Translation: CAA89786.1 .
    BK006946 Genomic DNA. Translation: DAA10189.1 .
    PIRi S54595.
    RefSeqi NP_014015.1. NM_001182795.1.

    3D structure databases

    ProteinModelPortali P49955.
    SMRi P49955. Positions 579-610, 647-711.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35468. 60 interactions.
    DIPi DIP-2628N.
    IntActi P49955. 27 interactions.
    MINTi MINT-426520.
    STRINGi 4932.YMR288W.

    Proteomic databases

    MaxQBi P49955.
    PaxDbi P49955.
    PeptideAtlasi P49955.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR288W ; YMR288W ; YMR288W .
    GeneIDi 855332.
    KEGGi sce:YMR288W.

    Organism-specific databases

    CYGDi YMR288w.
    SGDi S000004901. HSH155.

    Phylogenomic databases

    eggNOGi COG5181.
    GeneTreei ENSGT00390000018393.
    HOGENOMi HOG000166737.
    KOi K12828.
    OMAi AFWVRRM.
    OrthoDBi EOG754HXV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32958-MONOMER.
    Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

    Miscellaneous databases

    NextBioi 979058.
    PROi P49955.

    Gene expression databases

    Genevestigatori P49955.

    Family and domain databases

    Gene3Di 1.25.10.10. 3 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
      Wang Q., Rymond B.C.
      Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RDS3.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSF3B1_YEAST
    AccessioniPrimary (citable) accession number: P49955
    Secondary accession number(s): D6W0B5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 521 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3