Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P49955

- SF3B1_YEAST

UniProt

P49955 - SF3B1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

U2 snRNP component HSH155

Gene

HSH155

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Contacts pre-mRNA on both sides of the branch site early in spliceosome assembly.By similarity

GO - Molecular functioni

  1. mRNA binding Source: SGD

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
  2. spliceosomal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-32958-MONOMER.
ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 snRNP component HSH155
Gene namesi
Name:HSH155
Ordered Locus Names:YMR288W
ORF Names:YM8021.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR288w.
SGDiS000004901. HSH155.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. U2 snRNP Source: SGD
  2. U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971U2 snRNP component HSH155PRO_0000174327Add
BLAST

Proteomic databases

MaxQBiP49955.
PaxDbiP49955.
PeptideAtlasiP49955.

Expressioni

Gene expression databases

GenevestigatoriP49955.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RSE1Q046935EBI-664,EBI-519
YSF3P0C0743EBI-664,EBI-970846

Protein-protein interaction databases

BioGridi35468. 60 interactions.
DIPiDIP-2628N.
IntActiP49955. 27 interactions.
MINTiMINT-426520.
STRINGi4932.YMR288W.

Structurei

3D structure databases

ProteinModelPortaliP49955.
SMRiP49955. Positions 647-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati199 – 23739HEAT 1Add
BLAST
Repeati273 – 31038HEAT 2Add
BLAST
Repeati350 – 38738HEAT 3Add
BLAST
Repeati513 – 55038HEAT 4Add
BLAST
Repeati596 – 63338HEAT 5Add
BLAST
Repeati680 – 71738HEAT 6Add
BLAST
Repeati722 – 75938HEAT 7Add
BLAST
Repeati792 – 82938HEAT 8Add
BLAST
Repeati832 – 87039HEAT 9Add
BLAST

Sequence similaritiesi

Belongs to the SF3B1 family.Curated
Contains 9 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5181.
GeneTreeiENSGT00390000018393.
HOGENOMiHOG000166737.
InParanoidiP49955.
KOiK12828.
OMAiAFWVRRM.
OrthoDBiEOG754HXV.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

P49955 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE
60 70 80 90 100
KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR
110 120 130 140 150
WDVKGYEPPD ESSTAVKENS DSALVNVEGI HDLMFFKPSD HKYFADVISK
160 170 180 190 200
KPIDELNKDE KKERTLSMLL LKIKNGNTAS RRTSMRILTD KAVTFGPEMI
210 220 230 240 250
FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV HKILVVAAPL
260 270 280 290 300
LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA
310 320 330 340 350
AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN
360 370 380 390 400
HLTGLMSCIK DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL
410 420 430 440 450
WKGIRSHRGK VLSSFLKAVG SMIPLMDPEY AGYYTTEAMR IIRREFDSPD
460 470 480 490 500
DEMKKTILLV LQKCSAVESI TPKFLREEIA PEFFQKFWVR RVALDRPLNK
510 520 530 540 550
VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA VTRTVNLLGT
560 570 580 590 600
ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP
610 620 630 640 650
IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES
660 670 680 690 700
LGEVYPEVLG SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV
710 720 730 740 750
EVNTIKFVGL IGKLAPTYAP PKEWMRICFE LLELLKSTNK EIRRSANATF
760 770 780 790 800
GFIAEAIGPH DVLVALLNNL KVQERQLRVC TAVAIGIVAK VCGPYNVLPV
810 820 830 840 850
IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD
860 870 880 890 900
LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL
910 920 930 940 950
EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV
960 970
PFYPVTPDNN EEYIEELDLV L
Length:971
Mass (Da):110,028
Last modified:October 1, 1996 - v1
Checksum:i27D26E4252A788E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49704 Genomic DNA. Translation: CAA89786.1.
BK006946 Genomic DNA. Translation: DAA10189.1.
PIRiS54595.
RefSeqiNP_014015.1. NM_001182795.1.

Genome annotation databases

EnsemblFungiiYMR288W; YMR288W; YMR288W.
GeneIDi855332.
KEGGisce:YMR288W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49704 Genomic DNA. Translation: CAA89786.1 .
BK006946 Genomic DNA. Translation: DAA10189.1 .
PIRi S54595.
RefSeqi NP_014015.1. NM_001182795.1.

3D structure databases

ProteinModelPortali P49955.
SMRi P49955. Positions 647-711.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35468. 60 interactions.
DIPi DIP-2628N.
IntActi P49955. 27 interactions.
MINTi MINT-426520.
STRINGi 4932.YMR288W.

Proteomic databases

MaxQBi P49955.
PaxDbi P49955.
PeptideAtlasi P49955.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR288W ; YMR288W ; YMR288W .
GeneIDi 855332.
KEGGi sce:YMR288W.

Organism-specific databases

CYGDi YMR288w.
SGDi S000004901. HSH155.

Phylogenomic databases

eggNOGi COG5181.
GeneTreei ENSGT00390000018393.
HOGENOMi HOG000166737.
InParanoidi P49955.
KOi K12828.
OMAi AFWVRRM.
OrthoDBi EOG754HXV.

Enzyme and pathway databases

BioCyci YEAST:G3O-32958-MONOMER.
Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi 979058.
PROi P49955.

Gene expression databases

Genevestigatori P49955.

Family and domain databases

Gene3Di 1.25.10.10. 3 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
    Wang Q., Rymond B.C.
    Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RDS3.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSF3B1_YEAST
AccessioniPrimary (citable) accession number: P49955
Secondary accession number(s): D6W0B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3