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P49955

- SF3B1_YEAST

UniProt

P49955 - SF3B1_YEAST

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Protein
U2 snRNP component HSH155
Gene
HSH155, YMR288W, YM8021.14
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Contacts pre-mRNA on both sides of the branch site early in spliceosome assembly By similarity.

GO - Molecular functioni

  1. mRNA binding Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
  2. spliceosomal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-32958-MONOMER.
ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 snRNP component HSH155
Gene namesi
Name:HSH155
Ordered Locus Names:YMR288W
ORF Names:YM8021.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR288w.
SGDiS000004901. HSH155.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. U2 snRNP Source: SGD
  2. U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971U2 snRNP component HSH155
PRO_0000174327Add
BLAST

Proteomic databases

MaxQBiP49955.
PaxDbiP49955.
PeptideAtlasiP49955.

Expressioni

Gene expression databases

GenevestigatoriP49955.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RSE1Q046935EBI-664,EBI-519
YSF3P0C0743EBI-664,EBI-970846

Protein-protein interaction databases

BioGridi35468. 60 interactions.
DIPiDIP-2628N.
IntActiP49955. 27 interactions.
MINTiMINT-426520.
STRINGi4932.YMR288W.

Structurei

3D structure databases

ProteinModelPortaliP49955.
SMRiP49955. Positions 579-610, 647-711.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati199 – 23739HEAT 1
Add
BLAST
Repeati273 – 31038HEAT 2
Add
BLAST
Repeati350 – 38738HEAT 3
Add
BLAST
Repeati513 – 55038HEAT 4
Add
BLAST
Repeati596 – 63338HEAT 5
Add
BLAST
Repeati680 – 71738HEAT 6
Add
BLAST
Repeati722 – 75938HEAT 7
Add
BLAST
Repeati792 – 82938HEAT 8
Add
BLAST
Repeati832 – 87039HEAT 9
Add
BLAST

Sequence similaritiesi

Belongs to the SF3B1 family.
Contains 9 HEAT repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5181.
GeneTreeiENSGT00390000018393.
HOGENOMiHOG000166737.
KOiK12828.
OMAiAFWVRRM.
OrthoDBiEOG754HXV.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

P49955-1 [UniParc]FASTAAdd to Basket

« Hide

MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE    50
KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR 100
WDVKGYEPPD ESSTAVKENS DSALVNVEGI HDLMFFKPSD HKYFADVISK 150
KPIDELNKDE KKERTLSMLL LKIKNGNTAS RRTSMRILTD KAVTFGPEMI 200
FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV HKILVVAAPL 250
LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA 300
AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN 350
HLTGLMSCIK DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL 400
WKGIRSHRGK VLSSFLKAVG SMIPLMDPEY AGYYTTEAMR IIRREFDSPD 450
DEMKKTILLV LQKCSAVESI TPKFLREEIA PEFFQKFWVR RVALDRPLNK 500
VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA VTRTVNLLGT 550
ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP 600
IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES 650
LGEVYPEVLG SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV 700
EVNTIKFVGL IGKLAPTYAP PKEWMRICFE LLELLKSTNK EIRRSANATF 750
GFIAEAIGPH DVLVALLNNL KVQERQLRVC TAVAIGIVAK VCGPYNVLPV 800
IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD 850
LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL 900
EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV 950
PFYPVTPDNN EEYIEELDLV L 971
Length:971
Mass (Da):110,028
Last modified:October 1, 1996 - v1
Checksum:i27D26E4252A788E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49704 Genomic DNA. Translation: CAA89786.1.
BK006946 Genomic DNA. Translation: DAA10189.1.
PIRiS54595.
RefSeqiNP_014015.1. NM_001182795.1.

Genome annotation databases

EnsemblFungiiYMR288W; YMR288W; YMR288W.
GeneIDi855332.
KEGGisce:YMR288W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49704 Genomic DNA. Translation: CAA89786.1 .
BK006946 Genomic DNA. Translation: DAA10189.1 .
PIRi S54595.
RefSeqi NP_014015.1. NM_001182795.1.

3D structure databases

ProteinModelPortali P49955.
SMRi P49955. Positions 579-610, 647-711.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35468. 60 interactions.
DIPi DIP-2628N.
IntActi P49955. 27 interactions.
MINTi MINT-426520.
STRINGi 4932.YMR288W.

Proteomic databases

MaxQBi P49955.
PaxDbi P49955.
PeptideAtlasi P49955.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR288W ; YMR288W ; YMR288W .
GeneIDi 855332.
KEGGi sce:YMR288W.

Organism-specific databases

CYGDi YMR288w.
SGDi S000004901. HSH155.

Phylogenomic databases

eggNOGi COG5181.
GeneTreei ENSGT00390000018393.
HOGENOMi HOG000166737.
KOi K12828.
OMAi AFWVRRM.
OrthoDBi EOG754HXV.

Enzyme and pathway databases

BioCyci YEAST:G3O-32958-MONOMER.
Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi 979058.
PROi P49955.

Gene expression databases

Genevestigatori P49955.

Family and domain databases

Gene3Di 1.25.10.10. 3 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
    Wang Q., Rymond B.C.
    Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RDS3.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSF3B1_YEAST
AccessioniPrimary (citable) accession number: P49955
Secondary accession number(s): D6W0B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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