U2 snRNP component HSH155 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P49955 (SF3B1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
U2 snRNP component HSH155

Gene names

Name:	HSH155
Ordered Locus Names:	YMR288W
ORF Names:	YM8021.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	971 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contacts pre-mRNA on both sides of the branch site early in spliceosome assembly By similarity.
Subunit structure	Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3. Ref.3 Ref.4
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 521 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SF3B1 family. Contains 9 HEAT repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	spliceosomal complex assembly Inferred from physical interaction PubMed 10688664. Source: SGD
Cellular_component	U2 snRNP Inferred from direct assay PubMed 15565172 PubMed 16314500. Source: SGD U2-type prespliceosome Inferred from direct assay PubMed 16618970. Source: SGD
Molecular_function	mRNA binding Inferred from direct assay PubMed 12773561. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSE1	Q04693	5	EBI-664,EBI-519
YSF3	P0C074	3	EBI-664,EBI-970846

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 971

971

U2 snRNP component HSH155

PRO_0000174327

Regions

Repeat

199 – 237

HEAT 1

Repeat

273 – 310

HEAT 2

Repeat

350 – 387

HEAT 3

Repeat

513 – 550

HEAT 4

Repeat

596 – 633

HEAT 5

Repeat

680 – 717

HEAT 6

Repeat

722 – 759

HEAT 7

Repeat

792 – 829

HEAT 8

Repeat

832 – 870

HEAT 9

Amino acid modifications

Modified residue

Phosphothreonine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P49955 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 27D26E4252A788E2
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FASTA

971

110,028

        10         20         30         40         50         60 
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR 

        70         80         90        100        110        120 
EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR WDVKGYEPPD ESSTAVKENS 

       130        140        150        160        170        180 
DSALVNVEGI HDLMFFKPSD HKYFADVISK KPIDELNKDE KKERTLSMLL LKIKNGNTAS 

       190        200        210        220        230        240 
RRTSMRILTD KAVTFGPEMI FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV 

       250        260        270        280        290        300 
HKILVVAAPL LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA 

       310        320        330        340        350        360 
AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN HLTGLMSCIK 

       370        380        390        400        410        420 
DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL WKGIRSHRGK VLSSFLKAVG 

       430        440        450        460        470        480 
SMIPLMDPEY AGYYTTEAMR IIRREFDSPD DEMKKTILLV LQKCSAVESI TPKFLREEIA 

       490        500        510        520        530        540 
PEFFQKFWVR RVALDRPLNK VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA 

       550        560        570        580        590        600 
VTRTVNLLGT ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP 

       610        620        630        640        650        660 
IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES LGEVYPEVLG 

       670        680        690        700        710        720 
SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV EVNTIKFVGL IGKLAPTYAP 

       730        740        750        760        770        780 
PKEWMRICFE LLELLKSTNK EIRRSANATF GFIAEAIGPH DVLVALLNNL KVQERQLRVC 

       790        800        810        820        830        840 
TAVAIGIVAK VCGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP 

       850        860        870        880        890        900 
LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL 

       910        920        930        940        950        960 
EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV PFYPVTPDNN 

       970 
EEYIEELDLV L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[4]	"Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus." Wang Q., Rymond B.C. Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RDS3.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49704 Genomic DNA. Translation: CAA89786.1. BK006946 Genomic DNA. Translation: DAA10189.1.
PIR	S54595.
RefSeq	NP_014015.1. NM_001182795.1.
3D structure databases
ProteinModelPortal	P49955.
SMR	P49955. Positions 642-789.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2628N.
IntAct	P49955. 26 interactions.
MINT	MINT-426520.
STRING	4932.YMR288W.
Proteomic databases
PaxDb	P49955.
PeptideAtlas	P49955.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR288W; YMR288W; YMR288W.
GeneID	855332.
KEGG	sce:YMR288W.
Organism-specific databases
CYGD	YMR288w.
SGD	S000004901. HSH155.
Phylogenomic databases
eggNOG	COG5181.
GeneTree	ENSGT00390000018393.
HOGENOM	HOG000166737.
KO	K12828.
OMA	PKEWMRI.
OrthoDB	EOG4350F5.
Gene expression databases
Genevestigator	P49955.
GermOnline	YMR288W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 3 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979058.

Entry information

Entry name

SF3B1_YEAST

Accession

Primary (citable) accession number: P49955
Secondary accession number(s): D6W0B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents