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P49955 (SF3B1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U2 snRNP component HSH155
Gene names
Name:HSH155
Ordered Locus Names:YMR288W
ORF Names:YM8021.14
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length971 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contacts pre-mRNA on both sides of the branch site early in spliceosome assembly By similarity.

Subunit structure

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3. Ref.3 Ref.4

Subcellular location

Nucleus By similarity.

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SF3B1 family.

Contains 9 HEAT repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSE1Q046935EBI-664,EBI-519
YSF3P0C0743EBI-664,EBI-970846

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 971971U2 snRNP component HSH155
PRO_0000174327

Regions

Repeat199 – 23739HEAT 1
Repeat273 – 31038HEAT 2
Repeat350 – 38738HEAT 3
Repeat513 – 55038HEAT 4
Repeat596 – 63338HEAT 5
Repeat680 – 71738HEAT 6
Repeat722 – 75938HEAT 7
Repeat792 – 82938HEAT 8
Repeat832 – 87039HEAT 9

Sequences

Sequence LengthMass (Da)Tools
P49955 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 27D26E4252A788E2

FASTA971110,028
        10         20         30         40         50         60 
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR 

        70         80         90        100        110        120 
EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR WDVKGYEPPD ESSTAVKENS 

       130        140        150        160        170        180 
DSALVNVEGI HDLMFFKPSD HKYFADVISK KPIDELNKDE KKERTLSMLL LKIKNGNTAS 

       190        200        210        220        230        240 
RRTSMRILTD KAVTFGPEMI FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV 

       250        260        270        280        290        300 
HKILVVAAPL LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA 

       310        320        330        340        350        360 
AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN HLTGLMSCIK 

       370        380        390        400        410        420 
DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL WKGIRSHRGK VLSSFLKAVG 

       430        440        450        460        470        480 
SMIPLMDPEY AGYYTTEAMR IIRREFDSPD DEMKKTILLV LQKCSAVESI TPKFLREEIA 

       490        500        510        520        530        540 
PEFFQKFWVR RVALDRPLNK VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA 

       550        560        570        580        590        600 
VTRTVNLLGT ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP 

       610        620        630        640        650        660 
IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES LGEVYPEVLG 

       670        680        690        700        710        720 
SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV EVNTIKFVGL IGKLAPTYAP 

       730        740        750        760        770        780 
PKEWMRICFE LLELLKSTNK EIRRSANATF GFIAEAIGPH DVLVALLNNL KVQERQLRVC 

       790        800        810        820        830        840 
TAVAIGIVAK VCGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP 

       850        860        870        880        890        900 
LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL 

       910        920        930        940        950        960 
EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV PFYPVTPDNN 

       970 
EEYIEELDLV L 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
Wang Q., Rymond B.C.
Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RDS3.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49704 Genomic DNA. Translation: CAA89786.1.
BK006946 Genomic DNA. Translation: DAA10189.1.
PIRS54595.
RefSeqNP_014015.1. NM_001182795.1.

3D structure databases

ProteinModelPortalP49955.
SMRP49955. Positions 579-610, 647-711.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35468. 60 interactions.
DIPDIP-2628N.
IntActP49955. 27 interactions.
MINTMINT-426520.
STRING4932.YMR288W.

Proteomic databases

MaxQBP49955.
PaxDbP49955.
PeptideAtlasP49955.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR288W; YMR288W; YMR288W.
GeneID855332.
KEGGsce:YMR288W.

Organism-specific databases

CYGDYMR288w.
SGDS000004901. HSH155.

Phylogenomic databases

eggNOGCOG5181.
GeneTreeENSGT00390000018393.
HOGENOMHOG000166737.
KOK12828.
OMAAFWVRRM.
OrthoDBEOG754HXV.

Enzyme and pathway databases

BioCycYEAST:G3O-32958-MONOMER.

Gene expression databases

GenevestigatorP49955.

Family and domain databases

Gene3D1.25.10.10. 3 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
ProtoNetSearch...

Other

NextBio979058.
PROP49955.

Entry information

Entry nameSF3B1_YEAST
AccessionPrimary (citable) accession number: P49955
Secondary accession number(s): D6W0B5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families