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Protein

Probable hydrolase NIT3

Gene

NIT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei53Proton acceptorPROSITE-ProRule annotation1
Active sitei128Proton donorPROSITE-ProRule annotation1
Active sitei169NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YLR351C-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable hydrolase NIT3 (EC:3.5.-.-)
Gene namesi
Name:NIT3
Ordered Locus Names:YLR351C
ORF Names:L9638.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR351C.
SGDiS000004343. NIT3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002132561 – 291Probable hydrolase NIT3Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49954.
PRIDEiP49954.

PTM databases

iPTMnetiP49954.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31613. 8 interactors.
DIPiDIP-4666N.
IntActiP49954. 1 interactor.
MINTiMINT-477446.

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Beta strandi11 – 17Combined sources7
Helixi25 – 42Combined sources18
Beta strandi46 – 50Combined sources5
Turni53 – 56Combined sources4
Helixi61 – 67Combined sources7
Beta strandi73 – 75Combined sources3
Helixi78 – 89Combined sources12
Beta strandi93 – 95Combined sources3
Beta strandi99 – 102Combined sources4
Turni104 – 106Combined sources3
Beta strandi109 – 116Combined sources8
Beta strandi122 – 127Combined sources6
Helixi143 – 146Combined sources4
Beta strandi155 – 159Combined sources5
Beta strandi162 – 166Combined sources5
Helixi169 – 173Combined sources5
Helixi175 – 183Combined sources9
Beta strandi186 – 192Combined sources7
Helixi199 – 215Combined sources17
Beta strandi217 – 222Combined sources6
Beta strandi230 – 232Combined sources3
Beta strandi239 – 241Combined sources3
Beta strandi247 – 250Combined sources4
Beta strandi253 – 262Combined sources10
Helixi264 – 273Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F89X-ray2.40A/B1-291[»]
ProteinModelPortaliP49954.
SMRiP49954.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49954.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 286CN hydrolasePROSITE-ProRule annotationAdd BLAST276

Sequence similaritiesi

Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
InParanoidiP49954.
OMAiTISCLQF.
OrthoDBiEOG092C3EQE.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASKILSQK IKVALVQLSG SSPDKMANLQ RAATFIERAM KEQPDTKLVV
60 70 80 90 100
LPECFNSPYS TDQFRKYSEV INPKEPSTSV QFLSNLANKF KIILVGGTIP
110 120 130 140 150
ELDPKTDKIY NTSIIFNEDG KLIDKHRKVH LFDVDIPNGI SFHESETLSP
160 170 180 190 200
GEKSTTIDTK YGKFGVGICY DMRFPELAML SARKGAFAMI YPSAFNTVTG
210 220 230 240 250
PLHWHLLARS RAVDNQVYVM LCSPARNLQS SYHAYGHSIV VDPRGKIVAE
260 270 280 290
AGEGEEIIYA ELDPEVIESF RQAVPLTKQR RFDVYSDVNA H
Length:291
Mass (Da):32,549
Last modified:October 1, 1996 - v1
Checksum:iA813744120088827
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284572 Genomic DNA. Translation: AAF87101.1.
U19102 Genomic DNA. Translation: AAB67751.1.
BK006945 Genomic DNA. Translation: DAA09655.1.
PIRiS51459.
RefSeqiNP_013455.1. NM_001182240.1.

Genome annotation databases

EnsemblFungiiYLR351C; YLR351C; YLR351C.
GeneIDi851065.
KEGGisce:YLR351C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284572 Genomic DNA. Translation: AAF87101.1.
U19102 Genomic DNA. Translation: AAB67751.1.
BK006945 Genomic DNA. Translation: DAA09655.1.
PIRiS51459.
RefSeqiNP_013455.1. NM_001182240.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F89X-ray2.40A/B1-291[»]
ProteinModelPortaliP49954.
SMRiP49954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31613. 8 interactors.
DIPiDIP-4666N.
IntActiP49954. 1 interactor.
MINTiMINT-477446.

PTM databases

iPTMnetiP49954.

Proteomic databases

MaxQBiP49954.
PRIDEiP49954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR351C; YLR351C; YLR351C.
GeneIDi851065.
KEGGisce:YLR351C.

Organism-specific databases

EuPathDBiFungiDB:YLR351C.
SGDiS000004343. NIT3.

Phylogenomic databases

GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
InParanoidiP49954.
OMAiTISCLQF.
OrthoDBiEOG092C3EQE.

Enzyme and pathway databases

BioCyciYEAST:YLR351C-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP49954.
PROiP49954.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIT3_YEAST
AccessioniPrimary (citable) accession number: P49954
Secondary accession number(s): D6VYY9, Q71SP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.