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Protein

Probable hydrolase NIT3

Gene

NIT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei53 – 531Proton acceptorPROSITE-ProRule annotation
Active sitei128 – 1281Proton donorPROSITE-ProRule annotation
Active sitei169 – 1691NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YLR351C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable hydrolase NIT3 (EC:3.5.-.-)
Gene namesi
Name:NIT3
Ordered Locus Names:YLR351C
ORF Names:L9638.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR351C.
SGDiS000004343. NIT3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Probable hydrolase NIT3PRO_0000213256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49954.

PTM databases

iPTMnetiP49954.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31613. 8 interactions.
DIPiDIP-4666N.
IntActiP49954. 1 interaction.
MINTiMINT-477446.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Beta strandi11 – 177Combined sources
Helixi25 – 4218Combined sources
Beta strandi46 – 505Combined sources
Turni53 – 564Combined sources
Helixi61 – 677Combined sources
Beta strandi73 – 753Combined sources
Helixi78 – 8912Combined sources
Beta strandi93 – 953Combined sources
Beta strandi99 – 1024Combined sources
Turni104 – 1063Combined sources
Beta strandi109 – 1168Combined sources
Beta strandi122 – 1276Combined sources
Helixi143 – 1464Combined sources
Beta strandi155 – 1595Combined sources
Beta strandi162 – 1665Combined sources
Helixi169 – 1735Combined sources
Helixi175 – 1839Combined sources
Beta strandi186 – 1927Combined sources
Helixi199 – 21517Combined sources
Beta strandi217 – 2226Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi239 – 2413Combined sources
Beta strandi247 – 2504Combined sources
Beta strandi253 – 26210Combined sources
Helixi264 – 27310Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F89X-ray2.40A/B1-291[»]
ProteinModelPortaliP49954.
SMRiP49954. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49954.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 286276CN hydrolasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
InParanoidiP49954.
OMAiTISCLQF.
OrthoDBiEOG092C3EQE.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASKILSQK IKVALVQLSG SSPDKMANLQ RAATFIERAM KEQPDTKLVV
60 70 80 90 100
LPECFNSPYS TDQFRKYSEV INPKEPSTSV QFLSNLANKF KIILVGGTIP
110 120 130 140 150
ELDPKTDKIY NTSIIFNEDG KLIDKHRKVH LFDVDIPNGI SFHESETLSP
160 170 180 190 200
GEKSTTIDTK YGKFGVGICY DMRFPELAML SARKGAFAMI YPSAFNTVTG
210 220 230 240 250
PLHWHLLARS RAVDNQVYVM LCSPARNLQS SYHAYGHSIV VDPRGKIVAE
260 270 280 290
AGEGEEIIYA ELDPEVIESF RQAVPLTKQR RFDVYSDVNA H
Length:291
Mass (Da):32,549
Last modified:October 1, 1996 - v1
Checksum:iA813744120088827
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284572 Genomic DNA. Translation: AAF87101.1.
U19102 Genomic DNA. Translation: AAB67751.1.
BK006945 Genomic DNA. Translation: DAA09655.1.
PIRiS51459.
RefSeqiNP_013455.1. NM_001182240.1.

Genome annotation databases

EnsemblFungiiYLR351C; YLR351C; YLR351C.
GeneIDi851065.
KEGGisce:YLR351C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284572 Genomic DNA. Translation: AAF87101.1.
U19102 Genomic DNA. Translation: AAB67751.1.
BK006945 Genomic DNA. Translation: DAA09655.1.
PIRiS51459.
RefSeqiNP_013455.1. NM_001182240.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F89X-ray2.40A/B1-291[»]
ProteinModelPortaliP49954.
SMRiP49954. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31613. 8 interactions.
DIPiDIP-4666N.
IntActiP49954. 1 interaction.
MINTiMINT-477446.

PTM databases

iPTMnetiP49954.

Proteomic databases

MaxQBiP49954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR351C; YLR351C; YLR351C.
GeneIDi851065.
KEGGisce:YLR351C.

Organism-specific databases

EuPathDBiFungiDB:YLR351C.
SGDiS000004343. NIT3.

Phylogenomic databases

GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
InParanoidiP49954.
OMAiTISCLQF.
OrthoDBiEOG092C3EQE.

Enzyme and pathway databases

BioCyciYEAST:YLR351C-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP49954.
PROiP49954.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIT3_YEAST
AccessioniPrimary (citable) accession number: P49954
Secondary accession number(s): D6VYY9, Q71SP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.