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Protein

Wilms tumor protein homolog

Gene

Wt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei423 – 4231Important for interaction with target DNABy similarity
Sitei429 – 4291Important for interaction with target DNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri322 – 34625C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri352 – 37625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri382 – 40423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri413 – 43725C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Wilms tumor protein homolog
Gene namesi
Name:Wt1
Synonyms:Wt-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3974. Wt1.

Subcellular locationi

Isoform 1 :
Isoform 4 :

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nuclear speck Source: UniProtKB
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Wilms tumor protein homologPRO_0000047134Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki72 – 72Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki176 – 176Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP49952.
PRIDEiP49952.

Expressioni

Tissue specificityi

Kidney.

Developmental stagei

Expressed during kidney development.

Interactioni

Subunit structurei

Interacts with ZNF224 via the zinc-finger region. Interacts with WTAP, AMER1 and SRY. Homodimer. Interacts with WTIP. Interacts with actively translating polysomes. Detected in nuclear ribonucleoprotein (mRNP) particles. Interacts with U2AF2. Interacts with HNRNPU via the zinc-finger region. Interacts with CITED2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246993. 1 interaction.
STRINGi10116.ENSRNOP00000060038.

Structurei

3D structure databases

ProteinModelPortaliP49952.
SMRiP49952. Positions 317-437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni366 – 38015Important for interaction with target DNABy similarityAdd
BLAST
Regioni392 – 4009Important for interaction with target DNABy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi407 – 4093KTS motifBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi27 – 8256Pro-richAdd
BLAST

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri322 – 34625C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri352 – 37625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri382 – 40423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri413 – 43725C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000230937.
HOVERGENiHBG006960.
InParanoidiP49952.
KOiK09234.
PhylomeDBiP49952.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017987. Wilms_tumour.
IPR000976. Wilms_tumour_N.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02165. WT1. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
PRINTSiPR00049. WILMSTUMOUR.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49952-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSDVRDLNA LLPAVSSLGG GGGCGLPVSG ARQWAPVLDF APPGASAYGS
60 70 80 90 100
LGGPAPPPAP PPPPPPPHSF IKQEPSWGGA EPHEEQCLSA FTLHFSGQFT
110 120 130 140 150
GTAGACRYGP FGPPPPSQAS SGQARMFPNA PYLPSCLESQ PSIRNQGYST
160 170 180 190 200
VTFDGAPSYG HTPSHHAAQF PNHSFKHEDP MGQQGSLGEQ QYSVPPPVYG
210 220 230 240 250
CHTPTDSCTG SQALLLRTPY SSDNLYQMTS QLECMTWNQM NLGATLKGMA
260 270 280 290 300
AGSSSSVKWT EGQSNHGTGY ESENHTTPIL CGAQYRIHTH GVFRGIQDVR
310 320 330 340 350
RVSGVAPTLV RSASETSEKR PFMCAYPGCN KRYFKLSHLQ MHSRKHTGEK
360 370 380 390 400
PYQCDFKDCE RRFSRSDQLK RHQRRHTGVK PFQCKTCQRK FSRSDHLKTH
410 420 430 440
TRTHTGKTSE KPFSCRWHSC QKKFARSDEL VRHHNMHQRN MTKLHVAL
Length:448
Mass (Da):49,193
Last modified:October 1, 1996 - v1
Checksum:i329AC9AC1FF73F76
GO
Isoform 2 (identifier: P49952-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-265: Missing.
     407-409: Missing.

Show »
Length:428
Mass (Da):47,168
Checksum:i8C19A661FD632978
GO
Isoform 3 (identifier: P49952-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-265: Missing.

Show »
Length:431
Mass (Da):47,484
Checksum:iEAAB9CF4061F0894
GO
Isoform 4 (identifier: P49952-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     407-409: Missing.

Show »
Length:445
Mass (Da):48,877
Checksum:iF4004E561FE30C46
GO

RNA editingi

Edited at position 280.1 Publication
Partially edited.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti280 – 2801L → P in RNA edited version.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei249 – 26517Missing in isoform 2 and isoform 3. 1 PublicationVSP_006872Add
BLAST
Alternative sequencei407 – 4093Missing in isoform 2 and isoform 4. 1 PublicationVSP_006873

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69716 mRNA. Translation: CAA49373.1.
PIRiS33926.
RefSeqiNP_113722.2. NM_031534.2.
UniGeneiRn.92531.

Genome annotation databases

GeneIDi24883.
KEGGirno:24883.
UCSCiRGD:3974. rat. [P49952-1]

Keywords - Coding sequence diversityi

Alternative splicing, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69716 mRNA. Translation: CAA49373.1.
PIRiS33926.
RefSeqiNP_113722.2. NM_031534.2.
UniGeneiRn.92531.

3D structure databases

ProteinModelPortaliP49952.
SMRiP49952. Positions 317-437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246993. 1 interaction.
STRINGi10116.ENSRNOP00000060038.

Proteomic databases

PaxDbiP49952.
PRIDEiP49952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24883.
KEGGirno:24883.
UCSCiRGD:3974. rat. [P49952-1]

Organism-specific databases

CTDi7490.
RGDi3974. Wt1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000230937.
HOVERGENiHBG006960.
InParanoidiP49952.
KOiK09234.
PhylomeDBiP49952.

Miscellaneous databases

PROiP49952.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017987. Wilms_tumour.
IPR000976. Wilms_tumour_N.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02165. WT1. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
PRINTSiPR00049. WILMSTUMOUR.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWT1_RAT
AccessioniPrimary (citable) accession number: P49952
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Presence of the KTS motif hinders interactions between DNA and zinc-finger 4.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.