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Protein

Bacterioferritin

Gene

bfr

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex.By similarity

Catalytic activityi

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.By similarity
  • Fe cationBy similarityNote: Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Iron 1PROSITE-ProRule annotation
Metal bindingi51 – 511Iron 1PROSITE-ProRule annotation
Metal bindingi51 – 511Iron 2PROSITE-ProRule annotation
Metal bindingi52 – 521Iron (heme axial ligand); shared with dimeric partnerPROSITE-ProRule annotation
Metal bindingi54 – 541Iron 1PROSITE-ProRule annotation
Metal bindingi94 – 941Iron 2PROSITE-ProRule annotation
Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
Metal bindingi127 – 1271Iron 2PROSITE-ProRule annotation
Metal bindingi130 – 1301Iron 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Iron storage

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4193-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacterioferritin (EC:1.16.3.1)
Short name:
BFR
Gene namesi
Name:bfr
Ordered Locus Names:BMEII0704
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161BacterioferritinPRO_0000192589Add
BLAST

Interactioni

Subunit structurei

Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.By similarity

Protein-protein interaction databases

STRINGi224914.BAWG_1871.

Structurei

3D structure databases

ProteinModelPortaliP49944.
SMRiP49944. Positions 1-160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 145145Ferritin-like diironPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bacterioferritin family.Curated
Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000262383.
KOiK03594.
OMAiEMKHADQ.
OrthoDBiEOG6WDSKP.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
PRINTSiPR00601. BACFERRITIN.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR00754. bfr. 1 hit.
PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGEPKVIER LNDALFLELG AVNQYWLHYR LLNDWGYTRL AKKEREESIE
60 70 80 90 100
EMHHADKLIN RIIFFEGFPN LQTVSPLRIG QNVKEVLEAD LKGEYDARAS
110 120 130 140 150
YKESREICDK LGDYVSKQLF DELLADEEGH IDFLETQLDL LAKIGEERYG
160
QLNAAPADEA E
Length:161
Mass (Da):18,659
Last modified:October 1, 1996 - v1
Checksum:i5A92C1759B68BD1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19760 Genomic DNA. Translation: AAC43322.1.
AE008918 Genomic DNA. Translation: AAL53946.1.
PIRiAG3597.
S68771.
RefSeqiWP_004686876.1. NZ_GG703779.1.

Genome annotation databases

EnsemblBacteriaiAAL53946; AAL53946; BMEII0704.
KEGGibme:BMEII0704.
PATRICi17799658. VBIBruMel146950_2166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19760 Genomic DNA. Translation: AAC43322.1.
AE008918 Genomic DNA. Translation: AAL53946.1.
PIRiAG3597.
S68771.
RefSeqiWP_004686876.1. NZ_GG703779.1.

3D structure databases

ProteinModelPortaliP49944.
SMRiP49944. Positions 1-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_1871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL53946; AAL53946; BMEII0704.
KEGGibme:BMEII0704.
PATRICi17799658. VBIBruMel146950_2166.

Phylogenomic databases

HOGENOMiHOG000262383.
KOiK03594.
OMAiEMKHADQ.
OrthoDBiEOG6WDSKP.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4193-MONOMER.

Miscellaneous databases

PROiP49944.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
PRINTSiPR00601. BACFERRITIN.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR00754. bfr. 1 hit.
PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain."
    Denoel P.A., Zygmunt M.S., Weynants V.E., Tibor A., Lichtfouse B., Briffeuil P., Limet J.N., Letesson J.J.
    FEBS Lett. 361:238-242(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiBFR_BRUME
AccessioniPrimary (citable) accession number: P49944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.