P49944 (BFR_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bacterioferritin
Short name=BFR
EC=1.16.3.1

Gene names

Name:	bfr
Ordered Locus Names:	BMEII0704

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex By similarity.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center By similarity.
Subunit structure	Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited By similarity.
Sequence similarities	Belongs to the bacterioferritin family. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Bacterioferritin		PRO_0000192589
Regions
	Domain	1 – 145	145	Ferritin-like diiron
Sites
	Metal binding	18	1	Iron 1 By similarity
	Metal binding	51	1	Iron 1 By similarity
	Metal binding	51	1	Iron 2 By similarity
	Metal binding	52	1	Iron (heme axial ligand); shared with dimeric partner By similarity
	Metal binding	54	1	Iron 1 By similarity
	Metal binding	94	1	Iron 2 By similarity
	Metal binding	127	1	Iron 1 By similarity
	Metal binding	127	1	Iron 2 By similarity
	Metal binding	130	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49944 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5A92C1759B68BD1B	FASTA	161	18,659
	10 20 30 40 50 60 MKGEPKVIER LNDALFLELG AVNQYWLHYR LLNDWGYTRL AKKEREESIE EMHHADKLIN 70 80 90 100 110 120 RIIFFEGFPN LQTVSPLRIG QNVKEVLEAD LKGEYDARAS YKESREICDK LGDYVSKQLF 130 140 150 160 DELLADEEGH IDFLETQLDL LAKIGEERYG QLNAAPADEA E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain." Denoel P.A., Zygmunt M.S., Weynants V.E., Tibor A., Lichtfouse B., Briffeuil P., Limet J.N., Letesson J.J. FEBS Lett. 361:238-242(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19760 Genomic DNA. Translation: AAC43322.1. AE008918 Genomic DNA. Translation: AAL53946.1.
PIR	AG3597. S68771.
RefSeq	NP_541682.1. NC_003318.1.
3D structure databases
ProteinModelPortal	P49944.
SMR	P49944. Positions 1-160.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEII0704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL53946; AAL53946; BMEII0704.
GeneID	1198476.
KEGG	bme:BMEII0704.
PATRIC	17799658. VBIBruMel146950_2166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000262383.
KO	K03594.
OMA	HHIDFLE.
ProtClustDB	CLSK864320.
Family and domain databases
Gene3D	1.20.1260.10. 1 hit.
InterPro	IPR002024. Bacterioferritin. IPR009040. Ferritin-like_diiron. IPR009078. Ferritin-like_SF. IPR012347. Ferritin-rel. IPR008331. Ferritin_DPS_dom. [Graphical view]
Pfam	PF00210. Ferritin. 1 hit. [Graphical view]
PIRSF	PIRSF002560. Bacterioferritin. 1 hit.
PRINTS	PR00601. BACFERRITIN.
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR00754. bfr. 1 hit.
PROSITE	PS00549. BACTERIOFERRITIN. 1 hit. PS50905. FERRITIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BFR_BRUME

Accession

Primary (citable) accession number: P49944

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program