Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pro-cathepsin H

Gene

Ctsh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for the overall degradation of proteins in lysosomes.

Catalytic activityi

Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139By similarity1
Active sitei279By similarity1
Active sitei299By similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • cysteine-type endopeptidase activator activity involved in apoptotic process Source: UniProtKB
  • cysteine-type endopeptidase activity Source: UniProtKB
  • cysteine-type peptidase activity Source: UniProtKB
  • endopeptidase activity Source: UniProtKB
  • HLA-A specific activating MHC class I receptor activity Source: UniProtKB
  • peptidase activator activity involved in apoptotic process Source: MGI
  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: UniProtKB
  • thyroid hormone binding Source: UniProtKB

GO - Biological processi

  • bradykinin catabolic process Source: UniProtKB
  • cellular response to thyroid hormone stimulus Source: Ensembl
  • dichotomous subdivision of terminal units involved in lung branching Source: UniProtKB
  • ERK1 and ERK2 cascade Source: UniProtKB
  • immune response-regulating signaling pathway Source: UniProtKB
  • membrane protein proteolysis Source: UniProtKB
  • metanephros development Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • neuropeptide catabolic process Source: UniProtKB
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of apoptotic signaling pathway Source: MGI
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of epithelial cell migration Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of peptidase activity Source: UniProtKB
  • protein destabilization Source: UniProtKB
  • proteolysis Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • response to retinoic acid Source: UniProtKB
  • surfactant homeostasis Source: UniProtKB
  • T cell mediated cytotoxicity Source: UniProtKB
  • zymogen activation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiR-MMU-5683826. Surfactant metabolism.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-cathepsin H
Alternative name(s):
Cathepsin B3
Cathepsin BA
Cleaved into the following 4 chains:
Gene namesi
Name:Ctsh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:107285. Ctsh.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Disruption phenotypei

Mice exhibit markedly abnormal posterior chamber of eyeball with a configuration suggestive of increased axial lengthening, compared to the rounded appearance in wild-type littermates.1 Publication

Chemistry databases

ChEMBLiCHEMBL1949491.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002621221 – 95Activation peptideAdd BLAST75
ChainiPRO_000002621396 – 103Cathepsin H mini chainBy similarity8
PropeptideiPRO_0000026214104 – 113Sequence analysis10
ChainiPRO_0000026215114 – 333Cathepsin HBy similarityAdd BLAST220
ChainiPRO_0000026216114 – 290Cathepsin H heavy chainBy similarityAdd BLAST177
ChainiPRO_0000026217291 – 333Cathepsin H light chainBy similarityAdd BLAST43

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi100 ↔ 325By similarity
Disulfide bondi136 ↔ 179By similarity
Disulfide bondi170 ↔ 212By similarity
Glycosylationi228N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi270 ↔ 320By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP49935.
PaxDbiP49935.
PeptideAtlasiP49935.
PRIDEiP49935.

PTM databases

PhosphoSitePlusiP49935.

Expressioni

Tissue specificityi

Widely expressed with highest expression found in non-skeletal tissues. Low levels found in skeletal tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000032359.
CleanExiMM_CTSH.
ExpressionAtlasiP49935. baseline and differential.
GenevisibleiP49935. MM.

Interactioni

Subunit structurei

Composed of a mini chain and a large chain. The large chain may be split into heavy and light chain. All chains are held together by disulfide bonds.

Protein-protein interaction databases

IntActiP49935. 2 interactors.
MINTiMINT-4089943.
STRINGi10090.ENSMUSP00000034915.

Structurei

3D structure databases

ProteinModelPortaliP49935.
SMRiP49935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiP49935.
KOiK01366.
OMAiYSTEEYH.
OrthoDBiEOG091G0AKT.
TreeFamiTF328985.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAALPLLCA GAWLLSTGAT AELTVNAIEK FHFKSWMKQH QKTYSSVEYN
60 70 80 90 100
HRLQMFANNW RKIQAHNQRN HTFKMALNQF SDMSFAEIKH KFLWSEPQNC
110 120 130 140 150
SATKSNYLRG TGPYPSSMDW RKKGNVVSPV KNQGACGSCW TFSTTGALES
160 170 180 190 200
AVAIASGKML SLAEQQLVDC AQAFNNHGCK GGLPSQAFEY ILYNKGIMEE
210 220 230 240 250
DSYPYIGKDS SCRFNPQKAV AFVKNVVNIT LNDEAAMVEA VALYNPVSFA
260 270 280 290 300
FEVTEDFLMY KSGVYSSKSC HKTPDKVNHA VLAVGYGEQN GLLYWIVKNS
310 320 330
WGSQWGENGY FLIERGKNMC GLAACASYPI PQV
Length:333
Mass (Da):37,170
Last modified:July 27, 2011 - v2
Checksum:i96688394A87CCA36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137G → A in AAA82966 (PubMed:7722423).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06119 mRNA. Translation: AAA82966.1.
AK149949 mRNA. Translation: BAE29188.1.
AK150583 mRNA. Translation: BAE29677.1.
AK157376 mRNA. Translation: BAE34071.1.
AK160026 mRNA. Translation: BAE35569.1.
CH466560 Genomic DNA. Translation: EDL20901.1.
Y18464 mRNA. Translation: CAA77182.1.
CCDSiCCDS23399.1.
RefSeqiNP_001299578.1. NM_001312649.1.
NP_031827.2. NM_007801.3.
UniGeneiMm.2277.

Genome annotation databases

EnsembliENSMUST00000034915; ENSMUSP00000034915; ENSMUSG00000032359.
GeneIDi13036.
KEGGimmu:13036.
UCSCiuc009qzv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06119 mRNA. Translation: AAA82966.1.
AK149949 mRNA. Translation: BAE29188.1.
AK150583 mRNA. Translation: BAE29677.1.
AK157376 mRNA. Translation: BAE34071.1.
AK160026 mRNA. Translation: BAE35569.1.
CH466560 Genomic DNA. Translation: EDL20901.1.
Y18464 mRNA. Translation: CAA77182.1.
CCDSiCCDS23399.1.
RefSeqiNP_001299578.1. NM_001312649.1.
NP_031827.2. NM_007801.3.
UniGeneiMm.2277.

3D structure databases

ProteinModelPortaliP49935.
SMRiP49935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49935. 2 interactors.
MINTiMINT-4089943.
STRINGi10090.ENSMUSP00000034915.

Chemistry databases

ChEMBLiCHEMBL1949491.

Protein family/group databases

MEROPSiI29.003.

PTM databases

PhosphoSitePlusiP49935.

Proteomic databases

MaxQBiP49935.
PaxDbiP49935.
PeptideAtlasiP49935.
PRIDEiP49935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034915; ENSMUSP00000034915; ENSMUSG00000032359.
GeneIDi13036.
KEGGimmu:13036.
UCSCiuc009qzv.2. mouse.

Organism-specific databases

CTDi1512.
MGIiMGI:107285. Ctsh.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiP49935.
KOiK01366.
OMAiYSTEEYH.
OrthoDBiEOG091G0AKT.
TreeFamiTF328985.

Enzyme and pathway databases

ReactomeiR-MMU-5683826. Surfactant metabolism.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP49935.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032359.
CleanExiMM_CTSH.
ExpressionAtlasiP49935. baseline and differential.
GenevisibleiP49935. MM.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATH_MOUSE
AccessioniPrimary (citable) accession number: P49935
Secondary accession number(s): Q3UCD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.