Reviewed, UniProtKB/Swiss-Prot P49918 (CDN1C_HUMAN)

Names and origin

Protein names

Recommended name:
    Cyclin-dependent kinase inhibitor 1C
Alternative name(s):
    Cyclin-dependent kinase inhibitor p57
    p57KIP2

Gene names

Name:	CDKN1C
Synonyms:	KIP2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
Subcellular location	NucleusBy similarity.
Tissue specificity	Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
Involvement in disease	Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors. Defects in CDKN1C are involved in tumor formation.
Sequence similarities	Belongs to the CDI family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Disease mutation
Domain	Repeat
Molecular function	Anti-oncogene Protein kinase inhibitor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	G1 phase of mitotic cell cycle Ref.1 Traceable author statement. Source: ProtInc cell cycle arrest Ref.1 Traceable author statement. Source: ProtInc negative regulation of cell cycle Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of epithelial cell proliferation Inferred from mutant phenotype. Source: UniProtKB positive regulation of transforming growth factor beta receptor signaling pathway Inferred from mutant phenotype. Source: UniProtKB regulation of cyclin-dependent protein kinase activity Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: InterPro
Molecular function	cyclin-dependent protein kinase inhibitor activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK4	P11802	1	EBI-519256,EBI-295644

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P49918-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P49918-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Cyclin-dependent kinase inhibitor 1C		PRO_0000190087
Regions
	Repeat	156 – 159	4	1
	Repeat	160 – 163	4	2
	Repeat	180 – 183	4	3
	Repeat	184 – 187	4	4
	Repeat	188 – 191	4	5
	Repeat	198 – 201	4	6
	Repeat	202 – 205	4	7
	Repeat	206 – 209	4	8
	Repeat	210 – 213	4	9
	Region	156 – 213	58	9 X 4 AA repeats of P-A-P-A
	Motif	278 – 281	4	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	268	1	Phosphoserine
Natural variations
	Alternative sequence	1 – 11	11	Missing in isoform Short.		VSP_000867
	Natural variant	171 – 174	4	Missing in several cancers.		VAR_001404
	Natural variant	181 – 184	4	Missing in hepatocellular carcinomas.		VAR_001405
	Natural variant	200 – 203	4	Missing in a bladder cancer.		VAR_001406
	Natural variant	206 – 209	4	Missing in a breast cancer.		VAR_001407

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A94CCBF09F8CB3E4	FASTA	316	32,177
	10 20 30 40 50 60 MSDASLRSTS TMERLVARGT FPVLVRTSAC RSLFGPVDHE ELSRELQARL AELNAEDQNR 70 80 90 100 110 120 WDYDFQQDMP LRGPGRLQWT EVDSDSVPAF YRETVQVGRC RLLLAPRPVA VAVAVSPPLE 130 140 150 160 170 180 PAAESLDGLE EAPEQLPSVP VPAPASTPPP VPVLAPAPAP APAPVAAPVA APVAVAVLAP 190 200 210 220 230 240 APAPAPAPAP APAPVAAPAP APAPAPAPAP APAPAPDAAP QESAEQGANQ GQRGQEPLAD 250 260 270 280 290 300 QLHSGISGRP AAGTAAASAN GAAIKKLSGP LISDFFAKRK RSAPEKSSGD VPAPCPSPSA 310 APGVGSVEQT PRKRLR
	Isoform Short [UniParc]. Checksum: C4C82AF45384A1CC		305	31,040
	10 20 30 40 50 60 MERLVARGTF PVLVRTSACR SLFGPVDHEE LSRELQARLA ELNAEDQNRW DYDFQQDMPL 70 80 90 100 110 120 RGPGRLQWTE VDSDSVPAFY RETVQVGRCR LLLAPRPVAV AVAVSPPLEP AAESLDGLEE 130 140 150 160 170 180 APEQLPSVPV PAPASTPPPV PVLAPAPAPA PAPVAAPVAA PVAVAVLAPA PAPAPAPAPA 190 200 210 220 230 240 PAPVAAPAPA PAPAPAPAPA PAPAPDAAPQ ESAEQGANQG QRGQEPLADQ LHSGISGRPA 250 260 270 280 290 300 AGTAAASANG AAIKKLSGPL ISDFFAKRKR SAPEKSSGDV PAPCPSPSAA PGVGSVEQTP RKRLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"p57KIP2, a structurally distinct member of the p21CIP1 Cdk inhibitor family, is a candidate tumor suppressor gene." Matsuoka S., Edwards M.C., Bai C., Parker S., Zhang P., Baldini A., Harper J.W., Elledge S.J. Genes Dev. 9:650-662(1995) [PubMed: 7729684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Genomic organization of the human p57KIP2 gene and its analysis in the G401 Wilms' tumor assay." Reid L.H., Crider-Miller S.J., West A., Lee M.H., Massague J., Weissman B.E. Cancer Res. 56:1214-1218(1996) [PubMed: 8640800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Placenta.
[4]	"Characterization of the human p57KIP2 gene: alternative splicing, insertion/deletion polymorphisms in VNTR sequences in the coding region, and mutational analysis." Tokino T., Urano T., Furuhata T., Matsushima M., Miyatsu T., Sasaki S., Nakamura Y. Hum. Genet. 97:625-631(1996) [PubMed: 8655143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-316, VARIANTS.
[5]	"Analysis of germline CDKN1C (p57-KIP2) mutations in familial and sporadic Beckwith-Wiedemann syndrome (BWS) provides a novel genotype-phenotype correlation." Lam W.W.K., Hatada I., Ohishi S., Mukai T., Joyce J.A., Cole T.R.P., Donnai D., Reik W., Schofield P.N., Maher E.R. J. Med. Genet. 36:518-523(1999) [PubMed: 10424811] [Abstract] Cited for: INVOLVEMENT IN BWS.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

GeneReviews

Cross-references

Sequence databases
EMBLGenBankDDBJ	U22398 mRNA. Translation: AAA85095.1. U48869 Genomic DNA. Translation: AAB05896.1. BC067842 mRNA. Translation: AAH67842.1. D64137 Genomic DNA. Translation: BAA11014.1. D64137 Genomic DNA. Translation: BAA11015.1.
PIR	G02424.
RefSeq	NP_000067.1. NP_001116102.1. NP_001116103.1.
UniGene	Hs.106070
3D structure databases
DisProt	DP00017.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49918.
PTM databases
PhosphoSite	P49918.
Genome annotation databases
Ensembl	ENSG00000129757. Homo sapiens. [Contig view]
GeneID	1028.
KEGG	hsa:1028.
Organism-specific databases
H-InvDB	HIX0035977.
HGNC	HGNC:1786. CDKN1C.
HPA	HPA002924.
MIM	130650. phenotype. 600856. gene.
Orphanet	116. Beckwith-Wiedemann syndrome.
PharmGKB	PA26320.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P49918.
HOVERGEN	P49918.
Gene expression databases
ArrayExpress	P49918.
CleanEx	HS_CDKN1C.
GermOnline	ENSG00000129757. Homo sapiens.
Family and domain databases
InterPro	IPR003175. CDI. [Graphical view]
PANTHER	PTHR10265. CDI. 1 hit.
Pfam	PF02234. CDI. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4319.
SOURCE	Search...

Entry information

Entry name

CDN1C_HUMAN

Accession

Primary (citable) accession number: P49918

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)