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Protein

DNA ligase 3

Gene

LIG3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).2 Publications

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation2 Publications

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei506ATPCombined sourcesCurated1
Active sitei508N6-AMP-lysine intermediatePROSITE-ProRule annotation1 Publication1
Binding sitei513ATPCombined sourcesCurated1
Binding sitei528ATPBy similarity1
Metal bindingi560Magnesium 1By similarity1
Metal bindingi655Magnesium 2By similarity1
Binding sitei660ATPCombined sourcesCurated1
Binding sitei671ATPBy similarity1
Binding sitei675ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri93 – 185PARP-typePROSITE-ProRule annotation1 PublicationAdd BLAST93

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • DNA ligase (ATP) activity Source: BHF-UCL
  • DNA ligase activity Source: BHF-UCL
  • zinc ion binding Source: InterPro

GO - Biological processi

  • base-excision repair, DNA ligation Source: BHF-UCL
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • DNA biosynthetic process Source: InterPro
  • DNA ligation Source: BHF-UCL
  • DNA replication Source: UniProtKB-KW
  • double-strand break repair Source: BHF-UCL
  • double-strand break repair via alternative nonhomologous end joining Source: BHF-UCL
  • double-strand break repair via homologous recombination Source: Reactome
  • mitochondrial DNA repair Source: BHF-UCL
  • mitochondrion organization Source: BHF-UCL
  • negative regulation of mitochondrial DNA replication Source: CACAO
  • nucleotide-excision repair, DNA gap filling Source: Reactome
  • transcription-coupled nucleotide-excision repair Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS00130-MONOMER.
BRENDAi6.5.1.1. 2681.
ReactomeiR-HSA-110381. Resolution of AP sites via the single-nucleotide replacement pathway.
R-HSA-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.
R-HSA-5685939. HDR through MMEJ (alt-NHEJ).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
SIGNORiP49916.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 3 (EC:6.5.1.12 Publications)
Alternative name(s):
DNA ligase III
Polydeoxyribonucleotide synthase [ATP] 3
Gene namesi
Name:LIG3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:6600. LIG3.

Subcellular locationi

Isoform 1 :
Isoform 2 :
  • Mitochondrion 1 Publication

  • Note: Contains an N-terminal mitochondrial transit peptide.1 Publication
Isoform 3 :
  • Nucleus 1 Publication

  • Note: Lacks the N-terminal mitochondrial transit peptide.1 Publication
Isoform 4 :
  • Nucleus 1 Publication

  • Note: Lacks the N-terminal mitochondrial transit peptide.1 Publication

GO - Cellular componenti

  • DNA ligase III-XRCC1 complex Source: BHF-UCL
  • mitochondrion Source: BHF-UCL
  • nucleoplasm Source: Reactome
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi410K → E: Nearly abolishes ligase activity with blunt-ended DNA, but not with nicked DNA. 1 Publication1
Mutagenesisi414R → E: Abolishes ligase activity with blunt-ended DNA, but not with nicked DNA. 1 Publication1

Organism-specific databases

DisGeNETi3980.
OpenTargetsiENSG00000005156.
PharmGKBiPA30374.

Chemistry databases

DrugBankiDB00290. Bleomycin.

Polymorphism and mutation databases

BioMutaiLIG3.
DMDMi251757259.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000005957443 – 1009DNA ligase 3Add BLAST967

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210PhosphoserineCombined sources1
Modified residuei216PhosphoserineCombined sources1
Modified residuei227PhosphoserineCombined sources1
Modified residuei242PhosphoserineCombined sources1
Modified residuei913PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP49916.
MaxQBiP49916.
PaxDbiP49916.
PeptideAtlasiP49916.
PRIDEiP49916.

PTM databases

iPTMnetiP49916.
PhosphoSitePlusiP49916.

Expressioni

Tissue specificityi

Testis, thymus, prostate and heart.

Gene expression databases

BgeeiENSG00000005156.
CleanExiHS_LIG3.
ExpressionAtlasiP49916. baseline and differential.
GenevisibleiP49916. HS.

Organism-specific databases

HPAiHPA006723.

Interactioni

Subunit structurei

Isoform 3 interacts (via BRCT domain) with the nuclear DNA-repair protein XRCC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MAXP612442EBI-1753381,EBI-751711

Protein-protein interaction databases

BioGridi110168. 61 interactors.
IntActiP49916. 27 interactors.
MINTiMINT-4531178.
STRINGi9606.ENSP00000367787.

Structurei

Secondary structure

11009
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi92 – 97Combined sources6
Beta strandi117 – 124Combined sources8
Helixi140 – 149Combined sources10
Beta strandi152 – 154Combined sources3
Beta strandi161 – 165Combined sources5
Turni166 – 168Combined sources3
Helixi171 – 185Combined sources15
Beta strandi188 – 190Combined sources3
Helixi259 – 261Combined sources3
Helixi263 – 274Combined sources12
Helixi279 – 291Combined sources13
Helixi303 – 310Combined sources8
Turni312 – 314Combined sources3
Helixi323 – 334Combined sources12
Helixi338 – 344Combined sources7
Helixi345 – 347Combined sources3
Helixi350 – 359Combined sources10
Beta strandi362 – 364Combined sources3
Helixi374 – 385Combined sources12
Helixi390 – 401Combined sources12
Helixi407 – 416Combined sources10
Beta strandi421 – 423Combined sources3
Helixi426 – 431Combined sources6
Helixi437 – 443Combined sources7
Helixi447 – 459Combined sources13
Beta strandi485 – 488Combined sources4
Helixi492 – 498Combined sources7
Beta strandi503 – 507Combined sources5
Beta strandi511 – 519Combined sources9
Beta strandi522 – 526Combined sources5
Helixi535 – 537Combined sources3
Turni538 – 540Combined sources3
Helixi541 – 543Combined sources3
Helixi545 – 548Combined sources4
Beta strandi553 – 564Combined sources12
Turni566 – 568Combined sources3
Helixi574 – 577Combined sources4
Helixi579 – 584Combined sources6
Beta strandi590 – 600Combined sources11
Helixi610 – 620Combined sources11
Turni625 – 627Combined sources3
Beta strandi628 – 630Combined sources3
Beta strandi633 – 636Combined sources4
Helixi639 – 651Combined sources13
Beta strandi657 – 663Combined sources7
Beta strandi671 – 676Combined sources6
Turni678 – 680Combined sources3
Beta strandi688 – 698Combined sources11
Beta strandi710 – 716Combined sources7
Turni718 – 720Combined sources3
Beta strandi721 – 729Combined sources9
Helixi735 – 740Combined sources6
Turni741 – 743Combined sources3
Beta strandi780 – 787Combined sources8
Beta strandi789 – 791Combined sources3
Beta strandi795 – 797Combined sources3
Beta strandi800 – 804Combined sources5
Beta strandi816 – 819Combined sources4
Helixi822 – 830Combined sources9
Helixi924 – 927Combined sources4
Turni930 – 932Combined sources3
Beta strandi936 – 938Combined sources3
Beta strandi942 – 944Combined sources3
Helixi952 – 961Combined sources10
Helixi969 – 974Combined sources6
Beta strandi976 – 980Combined sources5
Beta strandi982 – 984Combined sources3
Beta strandi988 – 991Combined sources4
Helixi993 – 1002Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IMONMR-A924-1009[»]
1IN1NMR-A924-1009[»]
1UW0NMR-A88-204[»]
3L2PX-ray3.00A257-833[»]
3PC7X-ray1.65A/B924-1009[»]
3PC8X-ray2.31C/D924-1008[»]
3QVGX-ray2.26A/C924-1008[»]
ProteinModelPortaliP49916.
SMRiP49916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini933 – 1009BRCTPROSITE-ProRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 280Interaction with DNA1 Publication4
Regioni318 – 323Interaction with DNA1 Publication6
Regioni388 – 391Interaction with DNA1 Publication4
Regioni421 – 427Interaction with DNA1 Publication7

Domaini

The PARP-type zinc finger is required for DNA ligase activity.1 Publication

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 PARP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri93 – 185PARP-typePROSITE-ProRule annotation1 PublicationAdd BLAST93

Keywords - Domaini

Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG4437. Eukaryota.
COG1793. LUCA.
GeneTreeiENSGT00860000133792.
HOGENOMiHOG000007653.
HOVERGENiHBG005515.
InParanoidiP49916.
KOiK10776.
OMAiDCIYFNG.
OrthoDBiEOG091G0XBU.
PhylomeDBiP49916.
TreeFamiTF316220.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di1.10.3260.10. 1 hit.
3.30.1740.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR031916. LIG3_BRCT.
IPR012340. NA-bd_OB-fold.
IPR001510. Znf_PARP.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF16759. LIG3_BRCT. 1 hit.
PF00645. zf-PARP. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM01336. zf-PARP. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
PS00347. PARP_ZN_FINGER_1. 1 hit.
PS50064. PARP_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P49916-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLAFKIFFP QTLRALSRKE LCLFRKHHWR DVRQFSQWSE TDLLHGHPLF
60 70 80 90 100
LRRKPVLSFQ GSHLRSRATY LVFLPGLHVG LCSGPCEMAE QRFCVDYAKR
110 120 130 140 150
GTAGCKKCKE KIVKGVCRIG KVVPNPFSES GGDMKEWYHI KCMFEKLERA
160 170 180 190 200
RATTKKIEDL TELEGWEELE DNEKEQITQH IADLSSKAAG TPKKKAVVQA
210 220 230 240 250
KLTTTGQVTS PVKGASFVTS TNPRKFSGFS AKPNNSGEAP SSPTPKRSLS
260 270 280 290 300
SSKCDPRHKD CLLREFRKLC AMVADNPSYN TKTQIIQDFL RKGSAGDGFH
310 320 330 340 350
GDVYLTVKLL LPGVIKTVYN LNDKQIVKLF SRIFNCNPDD MARDLEQGDV
360 370 380 390 400
SETIRVFFEQ SKSFPPAAKS LLTIQEVDEF LLRLSKLTKE DEQQQALQDI
410 420 430 440 450
ASRCTANDLK CIIRLIKHDL KMNSGAKHVL DALDPNAYEA FKASRNLQDV
460 470 480 490 500
VERVLHNAQE VEKEPGQRRA LSVQASLMTP VQPMLAEACK SVEYAMKKCP
510 520 530 540 550
NGMFSEIKYD GERVQVHKNG DHFSYFSRSL KPVLPHKVAH FKDYIPQAFP
560 570 580 590 600
GGHSMILDSE VLLIDNKTGK PLPFGTLGVH KKAAFQDANV CLFVFDCIYF
610 620 630 640 650
NDVSLMDRPL CERRKFLHDN MVEIPNRIMF SEMKRVTKAL DLADMITRVI
660 670 680 690 700
QEGLEGLVLK DVKGTYEPGK RHWLKVKKDY LNEGAMADTA DLVVLGAFYG
710 720 730 740 750
QGSKGGMMSI FLMGCYDPGS QKWCTVTKCA GGHDDATLAR LQNELDMVKI
760 770 780 790 800
SKDPSKIPSW LKVNKIYYPD FIVPDPKKAA VWEITGAEFS KSEAHTADGI
810 820 830 840 850
SIRFPRCTRI RDDKDWKSAT NLPQLKELYQ LSKEKADFTV VAGDEGSSTT
860 870 880 890 900
GGSSEENKGP SGSAVSRKAP SKPSASTKKA EGKLSNSNSK DGNMQTAKPS
910 920 930 940 950
AMKVGEKLAT KSSPVKVGEK RKAADETLCQ TKVLLDIFTG VRLYLPPSTP
960 970 980 990 1000
DFSRLRRYFV AFDGDLVQEF DMTSATHVLG SRDKNPAAQQ VSPEWIWACI

RKRRLVAPC
Note: Produced by alternative splicing.
Length:1,009
Mass (Da):112,907
Last modified:July 7, 2009 - v2
Checksum:i0E4057E33C3F19A6
GO
Isoform 2 (identifier: P49916-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     933-1009: VLLDIFTGVR...IRKRRLVAPC → RRPASEQRGRTVPAGRR

Note: Produced by alternative splicing.
Show »
Length:949
Mass (Da):106,018
Checksum:i0C10E40E0686DB77
GO
Isoform 3 (identifier: P49916-3) [UniParc]FASTAAdd to basket
Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.

Note: Produced by alternative initiation of isoform 1.
Show »
Length:922
Mass (Da):102,691
Checksum:iFC3C60F988868808
GO
Isoform 4 (identifier: P49916-4) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.
     933-1009: VLLDIFTGVR...IRKRRLVAPC → RRPASEQRGRTVPAGRR

Note: Produced by alternative initiation of isoform 2.
Show »
Length:862
Mass (Da):95,802
Checksum:iF6A3C6B1422FD895
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020196224R → W.Corresponds to variant rs3744356dbSNPEnsembl.1
Natural variantiVAR_036513717D → N in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs757797167dbSNPEnsembl.1
Natural variantiVAR_072387768Y → H.1 PublicationCorresponds to variant rs200981995dbSNPEnsembl.1
Natural variantiVAR_018807867R → H.1 PublicationCorresponds to variant rs3136025dbSNPEnsembl.1
Natural variantiVAR_021938898K → T.Corresponds to variant rs4986974dbSNPEnsembl.1
Natural variantiVAR_020197986P → S.Corresponds to variant rs4986973dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0574641 – 87Missing in isoform 3 and isoform 4. CuratedAdd BLAST87
Alternative sequenceiVSP_001302933 – 1009VLLDI…LVAPC → RRPASEQRGRTVPAGRR in isoform 2 and isoform 4. 1 PublicationCuratedAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84740 mRNA. Translation: CAA59230.1.
U40671 mRNA. Translation: AAA85022.1.
HQ204826 Genomic DNA. Translation: ADP89974.1.
HQ204826 Genomic DNA. Translation: ADP89975.1.
HQ204827 Genomic DNA. Translation: ADP89976.1.
HQ204827 Genomic DNA. Translation: ADP89977.1.
HQ204828 Genomic DNA. Translation: ADP89978.1.
HQ204828 Genomic DNA. Translation: ADP89979.1.
HQ204829 Genomic DNA. Translation: ADP89980.1.
HQ204829 Genomic DNA. Translation: ADP89981.1.
HQ204830 Genomic DNA. Translation: ADP89982.1.
HQ204830 Genomic DNA. Translation: ADP89983.1.
HQ204831 Genomic DNA. Translation: ADP89984.1.
HQ204831 Genomic DNA. Translation: ADP89985.1.
HQ204832 Genomic DNA. Translation: ADP89986.1.
HQ204832 Genomic DNA. Translation: ADP89987.1.
HQ204833 Genomic DNA. Translation: ADP89988.1.
HQ204833 Genomic DNA. Translation: ADP89989.1.
HQ204834 Genomic DNA. Translation: ADP89990.1.
HQ204834 Genomic DNA. Translation: ADP89991.1.
HQ204835 Genomic DNA. Translation: ADP89992.1.
HQ204835 Genomic DNA. Translation: ADP89993.1.
HQ204836 Genomic DNA. Translation: ADP89994.1.
HQ204836 Genomic DNA. Translation: ADP89995.1.
HQ204837 Genomic DNA. Translation: ADP89996.1.
HQ204837 Genomic DNA. Translation: ADP89997.1.
HQ204838 Genomic DNA. Translation: ADP89998.1.
HQ204838 Genomic DNA. Translation: ADP89999.1.
HQ204839 Genomic DNA. Translation: ADP90000.1.
HQ204839 Genomic DNA. Translation: ADP90001.1.
HQ204840 Genomic DNA. Translation: ADP90002.1.
HQ204840 Genomic DNA. Translation: ADP90003.1.
HQ204841 Genomic DNA. Translation: ADP90004.1.
HQ204841 Genomic DNA. Translation: ADP90005.1.
HQ204842 Genomic DNA. Translation: ADP90006.1.
HQ204842 Genomic DNA. Translation: ADP90007.1.
HQ204843 Genomic DNA. Translation: ADP90008.1.
HQ204843 Genomic DNA. Translation: ADP90009.1.
HQ204844 Genomic DNA. Translation: ADP90010.1.
HQ204844 Genomic DNA. Translation: ADP90011.1.
HQ204845 Genomic DNA. Translation: ADP90012.1.
HQ204845 Genomic DNA. Translation: ADP90013.1.
HQ204846 Genomic DNA. Translation: ADP90014.1.
HQ204846 Genomic DNA. Translation: ADP90015.1.
HQ204847 Genomic DNA. Translation: ADP90016.1.
HQ204847 Genomic DNA. Translation: ADP90017.1.
HQ204848 Genomic DNA. Translation: ADP90018.1.
HQ204848 Genomic DNA. Translation: ADP90019.1.
HQ204849 Genomic DNA. Translation: ADP90020.1.
HQ204849 Genomic DNA. Translation: ADP90021.1.
HQ204850 Genomic DNA. Translation: ADP90022.1.
HQ204850 Genomic DNA. Translation: ADP90023.1.
HQ204851 Genomic DNA. Translation: ADP90024.1.
HQ204851 Genomic DNA. Translation: ADP90025.1.
HQ204852 Genomic DNA. Translation: ADP90026.1.
HQ204852 Genomic DNA. Translation: ADP90027.1.
HQ204853 Genomic DNA. Translation: ADP90028.1.
HQ204853 Genomic DNA. Translation: ADP90029.1.
HQ204854 Genomic DNA. Translation: ADP90030.1.
HQ204854 Genomic DNA. Translation: ADP90031.1.
HQ204855 Genomic DNA. Translation: ADP90032.1.
HQ204855 Genomic DNA. Translation: ADP90033.1.
HQ204856 Genomic DNA. Translation: ADP90034.1.
HQ204856 Genomic DNA. Translation: ADP90035.1.
HQ204857 Genomic DNA. Translation: ADP90036.1.
HQ204857 Genomic DNA. Translation: ADP90037.1.
HQ204858 Genomic DNA. Translation: ADP90038.1.
HQ204858 Genomic DNA. Translation: ADP90039.1.
HQ204859 Genomic DNA. Translation: ADP90040.1.
HQ204859 Genomic DNA. Translation: ADP90041.1.
HQ204860 Genomic DNA. Translation: ADP90042.1.
HQ204860 Genomic DNA. Translation: ADP90043.1.
HQ204861 Genomic DNA. Translation: ADP90044.1.
HQ204861 Genomic DNA. Translation: ADP90045.1.
HQ204862 Genomic DNA. Translation: ADP90046.1.
HQ204862 Genomic DNA. Translation: ADP90047.1.
HQ204863 Genomic DNA. Translation: ADP90048.1.
HQ204863 Genomic DNA. Translation: ADP90049.1.
HQ204864 Genomic DNA. Translation: ADP90050.1.
HQ204864 Genomic DNA. Translation: ADP90051.1.
HQ204865 Genomic DNA. Translation: ADP90052.1.
HQ204865 Genomic DNA. Translation: ADP90053.1.
AF491645 Genomic DNA. Translation: AAL91592.1.
AC004223 Genomic DNA. No translation available.
AC022903 Genomic DNA. No translation available.
CH471147 Genomic DNA. Translation: EAW80197.1.
CH471147 Genomic DNA. Translation: EAW80199.1.
BC068005 mRNA. Translation: AAH68005.1.
CCDSiCCDS11284.2. [P49916-1]
CCDS11285.2. [P49916-2]
PIRiI37292.
RefSeqiNP_002302.2. NM_002311.4. [P49916-2]
NP_039269.2. NM_013975.3. [P49916-1]
XP_016880113.1. XM_017024624.1. [P49916-1]
UniGeneiHs.100299.
Hs.556108.

Genome annotation databases

EnsembliENST00000262327; ENSP00000262327; ENSG00000005156. [P49916-2]
ENST00000378526; ENSP00000367787; ENSG00000005156. [P49916-1]
GeneIDi3980.
KEGGihsa:3980.
UCSCiuc002hij.4. human. [P49916-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

DNA ligase entry

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84740 mRNA. Translation: CAA59230.1.
U40671 mRNA. Translation: AAA85022.1.
HQ204826 Genomic DNA. Translation: ADP89974.1.
HQ204826 Genomic DNA. Translation: ADP89975.1.
HQ204827 Genomic DNA. Translation: ADP89976.1.
HQ204827 Genomic DNA. Translation: ADP89977.1.
HQ204828 Genomic DNA. Translation: ADP89978.1.
HQ204828 Genomic DNA. Translation: ADP89979.1.
HQ204829 Genomic DNA. Translation: ADP89980.1.
HQ204829 Genomic DNA. Translation: ADP89981.1.
HQ204830 Genomic DNA. Translation: ADP89982.1.
HQ204830 Genomic DNA. Translation: ADP89983.1.
HQ204831 Genomic DNA. Translation: ADP89984.1.
HQ204831 Genomic DNA. Translation: ADP89985.1.
HQ204832 Genomic DNA. Translation: ADP89986.1.
HQ204832 Genomic DNA. Translation: ADP89987.1.
HQ204833 Genomic DNA. Translation: ADP89988.1.
HQ204833 Genomic DNA. Translation: ADP89989.1.
HQ204834 Genomic DNA. Translation: ADP89990.1.
HQ204834 Genomic DNA. Translation: ADP89991.1.
HQ204835 Genomic DNA. Translation: ADP89992.1.
HQ204835 Genomic DNA. Translation: ADP89993.1.
HQ204836 Genomic DNA. Translation: ADP89994.1.
HQ204836 Genomic DNA. Translation: ADP89995.1.
HQ204837 Genomic DNA. Translation: ADP89996.1.
HQ204837 Genomic DNA. Translation: ADP89997.1.
HQ204838 Genomic DNA. Translation: ADP89998.1.
HQ204838 Genomic DNA. Translation: ADP89999.1.
HQ204839 Genomic DNA. Translation: ADP90000.1.
HQ204839 Genomic DNA. Translation: ADP90001.1.
HQ204840 Genomic DNA. Translation: ADP90002.1.
HQ204840 Genomic DNA. Translation: ADP90003.1.
HQ204841 Genomic DNA. Translation: ADP90004.1.
HQ204841 Genomic DNA. Translation: ADP90005.1.
HQ204842 Genomic DNA. Translation: ADP90006.1.
HQ204842 Genomic DNA. Translation: ADP90007.1.
HQ204843 Genomic DNA. Translation: ADP90008.1.
HQ204843 Genomic DNA. Translation: ADP90009.1.
HQ204844 Genomic DNA. Translation: ADP90010.1.
HQ204844 Genomic DNA. Translation: ADP90011.1.
HQ204845 Genomic DNA. Translation: ADP90012.1.
HQ204845 Genomic DNA. Translation: ADP90013.1.
HQ204846 Genomic DNA. Translation: ADP90014.1.
HQ204846 Genomic DNA. Translation: ADP90015.1.
HQ204847 Genomic DNA. Translation: ADP90016.1.
HQ204847 Genomic DNA. Translation: ADP90017.1.
HQ204848 Genomic DNA. Translation: ADP90018.1.
HQ204848 Genomic DNA. Translation: ADP90019.1.
HQ204849 Genomic DNA. Translation: ADP90020.1.
HQ204849 Genomic DNA. Translation: ADP90021.1.
HQ204850 Genomic DNA. Translation: ADP90022.1.
HQ204850 Genomic DNA. Translation: ADP90023.1.
HQ204851 Genomic DNA. Translation: ADP90024.1.
HQ204851 Genomic DNA. Translation: ADP90025.1.
HQ204852 Genomic DNA. Translation: ADP90026.1.
HQ204852 Genomic DNA. Translation: ADP90027.1.
HQ204853 Genomic DNA. Translation: ADP90028.1.
HQ204853 Genomic DNA. Translation: ADP90029.1.
HQ204854 Genomic DNA. Translation: ADP90030.1.
HQ204854 Genomic DNA. Translation: ADP90031.1.
HQ204855 Genomic DNA. Translation: ADP90032.1.
HQ204855 Genomic DNA. Translation: ADP90033.1.
HQ204856 Genomic DNA. Translation: ADP90034.1.
HQ204856 Genomic DNA. Translation: ADP90035.1.
HQ204857 Genomic DNA. Translation: ADP90036.1.
HQ204857 Genomic DNA. Translation: ADP90037.1.
HQ204858 Genomic DNA. Translation: ADP90038.1.
HQ204858 Genomic DNA. Translation: ADP90039.1.
HQ204859 Genomic DNA. Translation: ADP90040.1.
HQ204859 Genomic DNA. Translation: ADP90041.1.
HQ204860 Genomic DNA. Translation: ADP90042.1.
HQ204860 Genomic DNA. Translation: ADP90043.1.
HQ204861 Genomic DNA. Translation: ADP90044.1.
HQ204861 Genomic DNA. Translation: ADP90045.1.
HQ204862 Genomic DNA. Translation: ADP90046.1.
HQ204862 Genomic DNA. Translation: ADP90047.1.
HQ204863 Genomic DNA. Translation: ADP90048.1.
HQ204863 Genomic DNA. Translation: ADP90049.1.
HQ204864 Genomic DNA. Translation: ADP90050.1.
HQ204864 Genomic DNA. Translation: ADP90051.1.
HQ204865 Genomic DNA. Translation: ADP90052.1.
HQ204865 Genomic DNA. Translation: ADP90053.1.
AF491645 Genomic DNA. Translation: AAL91592.1.
AC004223 Genomic DNA. No translation available.
AC022903 Genomic DNA. No translation available.
CH471147 Genomic DNA. Translation: EAW80197.1.
CH471147 Genomic DNA. Translation: EAW80199.1.
BC068005 mRNA. Translation: AAH68005.1.
CCDSiCCDS11284.2. [P49916-1]
CCDS11285.2. [P49916-2]
PIRiI37292.
RefSeqiNP_002302.2. NM_002311.4. [P49916-2]
NP_039269.2. NM_013975.3. [P49916-1]
XP_016880113.1. XM_017024624.1. [P49916-1]
UniGeneiHs.100299.
Hs.556108.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IMONMR-A924-1009[»]
1IN1NMR-A924-1009[»]
1UW0NMR-A88-204[»]
3L2PX-ray3.00A257-833[»]
3PC7X-ray1.65A/B924-1009[»]
3PC8X-ray2.31C/D924-1008[»]
3QVGX-ray2.26A/C924-1008[»]
ProteinModelPortaliP49916.
SMRiP49916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110168. 61 interactors.
IntActiP49916. 27 interactors.
MINTiMINT-4531178.
STRINGi9606.ENSP00000367787.

Chemistry databases

DrugBankiDB00290. Bleomycin.

PTM databases

iPTMnetiP49916.
PhosphoSitePlusiP49916.

Polymorphism and mutation databases

BioMutaiLIG3.
DMDMi251757259.

Proteomic databases

EPDiP49916.
MaxQBiP49916.
PaxDbiP49916.
PeptideAtlasiP49916.
PRIDEiP49916.

Protocols and materials databases

DNASUi3980.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262327; ENSP00000262327; ENSG00000005156. [P49916-2]
ENST00000378526; ENSP00000367787; ENSG00000005156. [P49916-1]
GeneIDi3980.
KEGGihsa:3980.
UCSCiuc002hij.4. human. [P49916-1]

Organism-specific databases

CTDi3980.
DisGeNETi3980.
GeneCardsiLIG3.
HGNCiHGNC:6600. LIG3.
HPAiHPA006723.
MIMi600940. gene.
neXtProtiNX_P49916.
OpenTargetsiENSG00000005156.
PharmGKBiPA30374.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4437. Eukaryota.
COG1793. LUCA.
GeneTreeiENSGT00860000133792.
HOGENOMiHOG000007653.
HOVERGENiHBG005515.
InParanoidiP49916.
KOiK10776.
OMAiDCIYFNG.
OrthoDBiEOG091G0XBU.
PhylomeDBiP49916.
TreeFamiTF316220.

Enzyme and pathway databases

BioCyciZFISH:HS00130-MONOMER.
BRENDAi6.5.1.1. 2681.
ReactomeiR-HSA-110381. Resolution of AP sites via the single-nucleotide replacement pathway.
R-HSA-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.
R-HSA-5685939. HDR through MMEJ (alt-NHEJ).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
SIGNORiP49916.

Miscellaneous databases

ChiTaRSiLIG3. human.
EvolutionaryTraceiP49916.
GeneWikiiLIG3.
GenomeRNAii3980.
PROiP49916.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005156.
CleanExiHS_LIG3.
ExpressionAtlasiP49916. baseline and differential.
GenevisibleiP49916. HS.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di1.10.3260.10. 1 hit.
3.30.1740.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR031916. LIG3_BRCT.
IPR012340. NA-bd_OB-fold.
IPR001510. Znf_PARP.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF16759. LIG3_BRCT. 1 hit.
PF00645. zf-PARP. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM01336. zf-PARP. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
PS00347. PARP_ZN_FINGER_1. 1 hit.
PS50064. PARP_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI3_HUMAN
AccessioniPrimary (citable) accession number: P49916
Secondary accession number(s): E5KLB5
, E5KLB6, Q16714, Q6NVK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: November 30, 2016
This is version 185 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.